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The map label for this gene is braC [H]

Identifier: 91786926

GI number: 91786926

Start: 1053873

End: 1055027

Strand: Direct

Name: braC [H]

Synonym: Bpro_1027

Alternate gene names: 91786926

Gene position: 1053873-1055027 (Clockwise)

Preceding gene: 91786924

Following gene: 91786930

Centisome position: 20.27

GC content: 61.9

Gene sequence:

>1155_bases
ATGACCTTCAAGCGCAGAGCCATGATCGCCACGGCAGCGGCCTCGGCCCTTTTTCTCAGCCCTCAACTGTGGGCGCAGGC
CCAGCCCATCAAAATCTTCAACGTGGTCGAGCTCTCCGGCACCGGGGCAACGGCAGGCACCATGTTCAAGTCCGGCATTG
AACTGGCGGTCAAGGACGTCAATGCCTCCGGCGGCATCCTGGGGCGCAAGCTTGAGGTGACCAACATGGACACCCAGACC
AATCCCGGCATTGCCAAGGCGCTGGTGGTACGCGCCATTGATGACGGCGCGTTTGCCATCTTCGGCCCTGTCTTCAGCGG
CTCCATCCTGATCAGCCAGACCGAGACGCGGCGTGCCGAGGTGCCCAATTTCACGGCCGCTGCCGGGGCCCTGGTGACCC
AGCAGGGCAGCCCCTATATCTTTCGCACCGGGCTGACCCAGGTCAACAGCATGCCCAAGGCCGCACGCTACATCGCCCGA
AACCTGAAGATCAAGAAAATCGCCGTGCTGTTCGTCAACAACGACTTCGGCAAACCCGGGCGCGACGCCATCGTCGGTGC
CCTGAAAACCTTTGGTGTGGAGGTGGTGGCGGACATTTCCACCGAAGCCGGCCAGCTCGATTTCTCCGCGCCCATCATCA
AGGTGAAGCAGGCCGGCGCCGAGGCGGTGTTTGTCTATCTGAACGAAGAAGAGTCGGCACGGGCGGTGCGCGAACTGCGC
AAGCAAGGTTGGGACAAGCCCATCGTGGGCGACGTGACGCTGCTGAGCCAGAAGGTGATCGATTTGGCGGGCGACGCGGC
CAACGGCGTGGTCGGGCACTCCGAAATGTCGGCCGACGCGCCGATTGCCGGCGTGAAAGTGGTGCGCGACAAGTTCTTCA
AGGAATACAACTTCCTGCCCGACCACAACGGCATCAAGGGCTACCAGGGTGTTTACATGCTCAAGGCGGCGATCCAGAAA
GTCGGCAAGTTCGACCGCGTGGCGGTGGCCAAACAGTTGCACGGCATGACCATCAAGGTGGCGGACGAACCCGGCGTGCT
GATGGATGTCACCTTCGATGACAAGGGCGACATCGACCGCGAGAGCTTCCTCATCGAGATCAAGGGCGGCAAACCCGTGG
TCAAGGAAGTCCTGCCGCCTCTGGGCAAGAACTGA

Upstream 100 bases:

>100_bases
CCGAGTACTGTACACTATTGTCGCGAGGCCGGTATAAGCTTTCATTTCAGCCTTCATTTCCGGCACTCCGATAACTTTCA
GACCCTCAATGGAGACAAGC

Downstream 100 bases:

>100_bases
AAATTTGTAACTACTCAGTCCTTCCTGTCTTTGGGGCTGAGGCCCGCGGCTCCGAGTCCGCCTGCGCGGACTTGGACGGG
GCGAAGAGTCGCAGTGTCTC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTFKRRAMIATAAASALFLSPQLWAQAQPIKIFNVVELSGTGATAGTMFKSGIELAVKDVNASGGILGRKLEVTNMDTQT
NPGIAKALVVRAIDDGAFAIFGPVFSGSILISQTETRRAEVPNFTAAAGALVTQQGSPYIFRTGLTQVNSMPKAARYIAR
NLKIKKIAVLFVNNDFGKPGRDAIVGALKTFGVEVVADISTEAGQLDFSAPIIKVKQAGAEAVFVYLNEEESARAVRELR
KQGWDKPIVGDVTLLSQKVIDLAGDAANGVVGHSEMSADAPIAGVKVVRDKFFKEYNFLPDHNGIKGYQGVYMLKAAIQK
VGKFDRVAVAKQLHGMTIKVADEPGVLMDVTFDDKGDIDRESFLIEIKGGKPVVKEVLPPLGKN

Sequences:

>Translated_384_residues
MTFKRRAMIATAAASALFLSPQLWAQAQPIKIFNVVELSGTGATAGTMFKSGIELAVKDVNASGGILGRKLEVTNMDTQT
NPGIAKALVVRAIDDGAFAIFGPVFSGSILISQTETRRAEVPNFTAAAGALVTQQGSPYIFRTGLTQVNSMPKAARYIAR
NLKIKKIAVLFVNNDFGKPGRDAIVGALKTFGVEVVADISTEAGQLDFSAPIIKVKQAGAEAVFVYLNEEESARAVRELR
KQGWDKPIVGDVTLLSQKVIDLAGDAANGVVGHSEMSADAPIAGVKVVRDKFFKEYNFLPDHNGIKGYQGVYMLKAAIQK
VGKFDRVAVAKQLHGMTIKVADEPGVLMDVTFDDKGDIDRESFLIEIKGGKPVVKEVLPPLGKN
>Mature_383_residues
TFKRRAMIATAAASALFLSPQLWAQAQPIKIFNVVELSGTGATAGTMFKSGIELAVKDVNASGGILGRKLEVTNMDTQTN
PGIAKALVVRAIDDGAFAIFGPVFSGSILISQTETRRAEVPNFTAAAGALVTQQGSPYIFRTGLTQVNSMPKAARYIARN
LKIKKIAVLFVNNDFGKPGRDAIVGALKTFGVEVVADISTEAGQLDFSAPIIKVKQAGAEAVFVYLNEEESARAVRELRK
QGWDKPIVGDVTLLSQKVIDLAGDAANGVVGHSEMSADAPIAGVKVVRDKFFKEYNFLPDHNGIKGYQGVYMLKAAIQKV
GKFDRVAVAKQLHGMTIKVADEPGVLMDVTFDDKGDIDRESFLIEIKGGKPVVKEVLPPLGKN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=26.158038147139, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789867, Length=360, Percent_Identity=27.5, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41080; Mature: 40949

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKRRAMIATAAASALFLSPQLWAQAQPIKIFNVVELSGTGATAGTMFKSGIELAVKDV
CCCCHHHHHHHHHHHHHEECHHHHCCCCCEEEEEEEEECCCCCCHHHHHHCCCEEEEEEC
NASGGILGRKLEVTNMDTQTNPGIAKALVVRAIDDGAFAIFGPVFSGSILISQTETRRAE
CCCCCEEEEEEEEECCCCCCCCCHHHEEEEEEECCCCEEEEECCCCCEEEEEECCCHHCC
VPNFTAAAGALVTQQGSPYIFRTGLTQVNSMPKAARYIARNLKIKKIAVLFVNNDFGKPG
CCCCHHHCCEEEECCCCCEEEECCCHHHHCCHHHHHHHHHCCEEEEEEEEEEECCCCCCC
RDAIVGALKTFGVEVVADISTEAGQLDFSAPIIKVKQAGAEAVFVYLNEEESARAVRELR
HHHHHHHHHHCCEEEEEECCCCCCCEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHH
KQGWDKPIVGDVTLLSQKVIDLAGDAANGVVGHSEMSADAPIAGVKVVRDKFFKEYNFLP
HCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
DHNGIKGYQGVYMLKAAIQKVGKFDRVAVAKQLHGMTIKVADEPGVLMDVTFDDKGDIDR
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCC
ESFLIEIKGGKPVVKEVLPPLGKN
CEEEEEECCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TFKRRAMIATAAASALFLSPQLWAQAQPIKIFNVVELSGTGATAGTMFKSGIELAVKDV
CCCHHHHHHHHHHHHHEECHHHHCCCCCEEEEEEEEECCCCCCHHHHHHCCCEEEEEEC
NASGGILGRKLEVTNMDTQTNPGIAKALVVRAIDDGAFAIFGPVFSGSILISQTETRRAE
CCCCCEEEEEEEEECCCCCCCCCHHHEEEEEEECCCCEEEEECCCCCEEEEEECCCHHCC
VPNFTAAAGALVTQQGSPYIFRTGLTQVNSMPKAARYIARNLKIKKIAVLFVNNDFGKPG
CCCCHHHCCEEEECCCCCEEEECCCHHHHCCHHHHHHHHHCCEEEEEEEEEEECCCCCCC
RDAIVGALKTFGVEVVADISTEAGQLDFSAPIIKVKQAGAEAVFVYLNEEESARAVRELR
HHHHHHHHHHCCEEEEEECCCCCCCEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHH
KQGWDKPIVGDVTLLSQKVIDLAGDAANGVVGHSEMSADAPIAGVKVVRDKFFKEYNFLP
HCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
DHNGIKGYQGVYMLKAAIQKVGKFDRVAVAKQLHGMTIKVADEPGVLMDVTFDDKGDIDR
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCC
ESFLIEIKGGKPVVKEVLPPLGKN
CEEEEEECCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]