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The map label for this gene is dhbF [H]

Identifier: 78049591

GI number: 78049591

Start: 4633071

End: 4634858

Strand: Reverse

Name: dhbF [H]

Synonym: XCV4035

Alternate gene names: 78049591

Gene position: 4634858-4633071 (Counterclockwise)

Preceding gene: 78049592

Following gene: 78049590

Centisome position: 89.5

GC content: 67.34

Gene sequence:

>1788_bases
TTGTACGGGCCTACCGAAACCACGATCTGGTCGACCATCATGCCGTTGCAGTTGACCGATGCCGCCGCACCGCCGATCGG
CAGGCCGCTGCTCAATACGCGCGTCTATGTGCTGGATGCGCAGCGGCAACCGCTGCCGACCGGTGCGATCGGCGAGCTGT
ATATCGGTGGCGCAGGCGTGGCCAAAGGCTATCGCGGCAAGCGCCAGCTGACCAGCGAGCGCTTCATGCGCGACCCGTTC
GCCGACGACGGCAGCAGCATGTACCGCACCGGCGACCGCGTGCGTCAACGCAGCGATGGCCTGCTGGAATTCATCGGGCG
CGCCGATGCGCAGCTGAAGATCCGCGGACATCGGGTGGAGCCGGCCGAAATCGAAAGCGCCCTGCTGTTGCATTCGCAGG
TGGCGCAGGCGGTGGTGGTGGGCCACGGCGATGGCGAGCATGCCTTGCAACTGCTGGCCTATGTGGTGGGCAAGCACGGC
AATGCGCCGTCGATCGAGTTGTTGCGCGCGCATCTGCAGCAGCGCCTGCCGGCATCGATGATTCCCACCCTGTGGGTGCC
GCTGGAGGCGTTGCCGCTGACCCCCAACGGCAAGCTGGACCGGCGCGCGTTGCCGGCGCCTGGCGCATTGCCGCAACGCG
CGCATCTGGCGCCGCGCGATGCGCTCGAACGCCAGCTGGCAACGATGCTGCAGGAGGTGCTGGGCATCGAAGTGGTGGGC
GTGGACGACAATTTCTTCGAGCTTGGCGGCGATTCGCTGCGTGCGGCGGAAATGGCCGCGCGTTTCCCGCAGATGTTCGG
GGTGGAACTGCCGCTGGGAACATTGTTCCATGCGCCGACGGTCGCCGGCCTGGCCGAGGTGATCAAGCGCAGCGCACACA
CGCCCAACGACCCGCTGAGCGTGCTGTTGCCGCTGCGTGCAGGCACCGCCGGCAGCACGCCGCTGTTCTGCATCCATCCG
GTCATCGGTCTGGGCTGGTCGTATCTCACCTTGCTGGGTCAGCTGGATCCGCAGTGGCCGGTGTATGCGCTGCAATCGCC
GGGCTTGAGCGACCCGCAGCACCGTCCGGAGAGCATCGAGGCGATCGCGCGCGATTACCTGGCGCGTCTGCGCAGCGTGC
AGCCGAGGGGGCCGTACCGTCTGCTGGGCTGGTCGCTGGGCGGTTTGATCGCGCACGCCATCACCGCCGAATTGCATGCG
CTGGGCGAAGAGGTCGAGGTGTTGGCCATGCTCGACAGTTACCCGCATCTGGAACAGGCCGCAGCGTTGTCGGAATCGGA
GAACGTGCGCGCCTCGGTGCACGCGTTGGCCTTGCATCTGGCGCCGGGCCAGCCGATGCCGACCACGGTCGCCGAGCTGG
CGAGCCTGTTGTGCGATCACTACGGGCTGAACGAATTGCCTGCGGTGCGCCAGTTGCTGGAGCAACGTCCCACCTTGCTG
GACGATCTGGGCGCCCTCACCCAACATCACCTGCAGCTGGCGCGGCGTCATCGGCCCAGGCAGCTGGACCAGGATGTGGT
CTTTGTCGAAGCGAGCGTGCGCATGAGCTCCGGCGGCGACGATGCGCTGTGGGTGGATTACCGTCCCCAGGCGTGGCACC
CGTACGTGCGCAACCTGCAGCTGCACGTTCTGGACAGCGACCACCATTCAATGCTCTCGCCGACGCATGCGGCCACGCTC
AGCACCTTGCTGCACGCGGCCACCGCAGAGGCCACCCGGGCTGTGCGGCCGGTGGGACAGGCCGGCCGTGGCGCGGTCGC
CGGCGACGGAGTGACCGAATATGCCTGA

Upstream 100 bases:

>100_bases
ATCTGGTGCTGGATCGCATTCATGCGTTGATCGGCGGCGAGGCGTTGGTGCCGGAACTGGCCACCCAACTGCTCAGCCGG
GTGTGGGGCGCCTGACCCAG

Downstream 100 bases:

>100_bases
GTCCAGCGCCGATCCGTTACGCCAGCGCCCGGTGGTGATCGCCACCCTTGGCACGCATGGGGACGTGCGCCCGATCATTG
CGCTGGCCCTTGGCCTGCAG

Product: putative non-ribosomal peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MYGPTETTIWSTIMPLQLTDAAAPPIGRPLLNTRVYVLDAQRQPLPTGAIGELYIGGAGVAKGYRGKRQLTSERFMRDPF
ADDGSSMYRTGDRVRQRSDGLLEFIGRADAQLKIRGHRVEPAEIESALLLHSQVAQAVVVGHGDGEHALQLLAYVVGKHG
NAPSIELLRAHLQQRLPASMIPTLWVPLEALPLTPNGKLDRRALPAPGALPQRAHLAPRDALERQLATMLQEVLGIEVVG
VDDNFFELGGDSLRAAEMAARFPQMFGVELPLGTLFHAPTVAGLAEVIKRSAHTPNDPLSVLLPLRAGTAGSTPLFCIHP
VIGLGWSYLTLLGQLDPQWPVYALQSPGLSDPQHRPESIEAIARDYLARLRSVQPRGPYRLLGWSLGGLIAHAITAELHA
LGEEVEVLAMLDSYPHLEQAAALSESENVRASVHALALHLAPGQPMPTTVAELASLLCDHYGLNELPAVRQLLEQRPTLL
DDLGALTQHHLQLARRHRPRQLDQDVVFVEASVRMSSGGDDALWVDYRPQAWHPYVRNLQLHVLDSDHHSMLSPTHAATL
STLLHAATAEATRAVRPVGQAGRGAVAGDGVTEYA

Sequences:

>Translated_595_residues
MYGPTETTIWSTIMPLQLTDAAAPPIGRPLLNTRVYVLDAQRQPLPTGAIGELYIGGAGVAKGYRGKRQLTSERFMRDPF
ADDGSSMYRTGDRVRQRSDGLLEFIGRADAQLKIRGHRVEPAEIESALLLHSQVAQAVVVGHGDGEHALQLLAYVVGKHG
NAPSIELLRAHLQQRLPASMIPTLWVPLEALPLTPNGKLDRRALPAPGALPQRAHLAPRDALERQLATMLQEVLGIEVVG
VDDNFFELGGDSLRAAEMAARFPQMFGVELPLGTLFHAPTVAGLAEVIKRSAHTPNDPLSVLLPLRAGTAGSTPLFCIHP
VIGLGWSYLTLLGQLDPQWPVYALQSPGLSDPQHRPESIEAIARDYLARLRSVQPRGPYRLLGWSLGGLIAHAITAELHA
LGEEVEVLAMLDSYPHLEQAAALSESENVRASVHALALHLAPGQPMPTTVAELASLLCDHYGLNELPAVRQLLEQRPTLL
DDLGALTQHHLQLARRHRPRQLDQDVVFVEASVRMSSGGDDALWVDYRPQAWHPYVRNLQLHVLDSDHHSMLSPTHAATL
STLLHAATAEATRAVRPVGQAGRGAVAGDGVTEYA
>Mature_595_residues
MYGPTETTIWSTIMPLQLTDAAAPPIGRPLLNTRVYVLDAQRQPLPTGAIGELYIGGAGVAKGYRGKRQLTSERFMRDPF
ADDGSSMYRTGDRVRQRSDGLLEFIGRADAQLKIRGHRVEPAEIESALLLHSQVAQAVVVGHGDGEHALQLLAYVVGKHG
NAPSIELLRAHLQQRLPASMIPTLWVPLEALPLTPNGKLDRRALPAPGALPQRAHLAPRDALERQLATMLQEVLGIEVVG
VDDNFFELGGDSLRAAEMAARFPQMFGVELPLGTLFHAPTVAGLAEVIKRSAHTPNDPLSVLLPLRAGTAGSTPLFCIHP
VIGLGWSYLTLLGQLDPQWPVYALQSPGLSDPQHRPESIEAIARDYLARLRSVQPRGPYRLLGWSLGGLIAHAITAELHA
LGEEVEVLAMLDSYPHLEQAAALSESENVRASVHALALHLAPGQPMPTTVAELASLLCDHYGLNELPAVRQLLEQRPTLL
DDLGALTQHHLQLARRHRPRQLDQDVVFVEASVRMSSGGDDALWVDYRPQAWHPYVRNLQLHVLDSDHHSMLSPTHAATL
STLLHAATAEATRAVRPVGQAGRGAVAGDGVTEYA

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=307, Percent_Identity=26.0586319218241, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI156151445, Length=205, Percent_Identity=27.3170731707317, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786801, Length=427, Percent_Identity=39.344262295082, Blast_Score=265, Evalue=8e-72,
Organism=Escherichia coli, GI1790505, Length=213, Percent_Identity=32.8638497652582, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786810, Length=192, Percent_Identity=28.6458333333333, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693145, Length=169, Percent_Identity=28.9940828402367, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17550940, Length=420, Percent_Identity=23.8095238095238, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556356, Length=307, Percent_Identity=29.9674267100977, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=204, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=360, Percent_Identity=27.7777777777778, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319699, Length=204, Percent_Identity=28.4313725490196, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648676, Length=281, Percent_Identity=32.3843416370107, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581924, Length=208, Percent_Identity=29.3269230769231, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 64574; Mature: 64574

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGPTETTIWSTIMPLQLTDAAAPPIGRPLLNTRVYVLDAQRQPLPTGAIGELYIGGAGV
CCCCCCCHHHHHCCCEEECCCCCCCCCCCHHCCEEEEEECCCCCCCCCCCCCEEECCCHH
AKGYRGKRQLTSERFMRDPFADDGSSMYRTGDRVRQRSDGLLEFIGRADAQLKIRGHRVE
HCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PAEIESALLLHSQVAQAVVVGHGDGEHALQLLAYVVGKHGNAPSIELLRAHLQQRLPASM
HHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHH
IPTLWVPLEALPLTPNGKLDRRALPAPGALPQRAHLAPRDALERQLATMLQEVLGIEVVG
CCHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEE
VDDNFFELGGDSLRAAEMAARFPQMFGVELPLGTLFHAPTVAGLAEVIKRSAHTPNDPLS
CCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCCCCCCCE
VLLPLRAGTAGSTPLFCIHPVIGLGWSYLTLLGQLDPQWPVYALQSPGLSDPQHRPESIE
EEEEEECCCCCCCCEEEECHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHH
AIARDYLARLRSVQPRGPYRLLGWSLGGLIAHAITAELHALGEEVEVLAMLDSYPHLEQA
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AALSESENVRASVHALALHLAPGQPMPTTVAELASLLCDHYGLNELPAVRQLLEQRPTLL
HHHCCCCCHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHH
DDLGALTQHHLQLARRHRPRQLDQDVVFVEASVRMSSGGDDALWVDYRPQAWHPYVRNLQ
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCHHHCCEE
LHVLDSDHHSMLSPTHAATLSTLLHAATAEATRAVRPVGQAGRGAVAGDGVTEYA
EEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYGPTETTIWSTIMPLQLTDAAAPPIGRPLLNTRVYVLDAQRQPLPTGAIGELYIGGAGV
CCCCCCCHHHHHCCCEEECCCCCCCCCCCHHCCEEEEEECCCCCCCCCCCCCEEECCCHH
AKGYRGKRQLTSERFMRDPFADDGSSMYRTGDRVRQRSDGLLEFIGRADAQLKIRGHRVE
HCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
PAEIESALLLHSQVAQAVVVGHGDGEHALQLLAYVVGKHGNAPSIELLRAHLQQRLPASM
HHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHH
IPTLWVPLEALPLTPNGKLDRRALPAPGALPQRAHLAPRDALERQLATMLQEVLGIEVVG
CCHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEE
VDDNFFELGGDSLRAAEMAARFPQMFGVELPLGTLFHAPTVAGLAEVIKRSAHTPNDPLS
CCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCCCCCCCE
VLLPLRAGTAGSTPLFCIHPVIGLGWSYLTLLGQLDPQWPVYALQSPGLSDPQHRPESIE
EEEEEECCCCCCCCEEEECHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHH
AIARDYLARLRSVQPRGPYRLLGWSLGGLIAHAITAELHALGEEVEVLAMLDSYPHLEQA
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AALSESENVRASVHALALHLAPGQPMPTTVAELASLLCDHYGLNELPAVRQLLEQRPTLL
HHHCCCCCHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHH
DDLGALTQHHLQLARRHRPRQLDQDVVFVEASVRMSSGGDDALWVDYRPQAWHPYVRNLQ
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCHHHCCEE
LHVLDSDHHSMLSPTHAATLSTLLHAATAEATRAVRPVGQAGRGAVAGDGVTEYA
EEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]