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The map label for this gene is phnJ [H]

Identifier: 66045494

GI number: 66045494

Start: 2619171

End: 2620058

Strand: Direct

Name: phnJ [H]

Synonym: Psyr_2254

Alternate gene names: 66045494

Gene position: 2619171-2620058 (Clockwise)

Preceding gene: 66045493

Following gene: 66045495

Centisome position: 42.98

GC content: 61.15

Gene sequence:

>888_bases
ATGACTCATGCACCGATGAACACCGCACAGCAAAGGCCTGCACGGGACGAGGCCTACAACTTCGCTTATCTGGACGAGCA
GACCAAACGCATGATTCGCCGCGCCTTGCTCAAGGCGGTGGCGATCCCCGGTTATCAGGTGCCGTTCGGCGGCCGCGAAA
TGCCGCTGCCGTATGGCTGGGGCACCGGCGGGATGCAACTGACCGCGGCGATCCTGGGCGCCGATGATGTGCTCAAGGTC
ATCGACCAGGGCGCTGACGACACCACCAACGCGGTCTCGATCAGGCGTTTCTTTGCCCGCACGGCCGGCATCGCCACCAC
CGAGCGCACGCCTGAGGCGACGGTCATCCAGACCCGTCACCGGATCCCGGAAACACCGCTGCACGCCGATCAGATCATGG
TCTATCAGGTGCCGATTCCCGAACCGCTGCGTTTCATCGAGCCCTCGGAAACCGAGACCCGGACCATGCACGCGCTGGAT
GATTACGGCGTCATGCACGTCAAACTCTACGAGGACATCGCCACTTTCGGCCATATCGCCACCAGTTACGCTTATCCAGT
GATGGTCGATGAACGCTATGTGATGGACCCGTCGCCGATTCCCAAATTCGATAACCCCAAGCTGCACATGAGCCCGGCAC
TGATGCTGTTCGGCGCGGGGCGCGAAAAGCGTCTGTACGCCGTGCCGCCGTATACGCAGGTGGTGAGCCTGGACTTTGAA
GATCACCCGTTCGAGGTGCAGCGCTGGGAGCAGTGCTGTGCGATCTGTGGCAGCCATGATTCCTTTCTCGACGAGCTGAT
TCTCGACGATGCCGGGACGCAGAGCTTTGTCTGTTCCGACACCGATTACTGCGCCCAGCGCGTCAAACAGCAGGAGAACG
ACCGATGA

Upstream 100 bases:

>100_bases
TCGTCTCGCACCTCAAGCTGCCGCATTACGTGGACTTCCAGTCCGAACTGGAGCTGATTCGCAAGCTGCGCACGTCTGCA
CCGAAACCGGGGAGCGACCA

Downstream 100 bases:

>100_bases
TCAGTGCACGTTCACTGGACCCGGCGGGCGAGCGCCTTCCCGAGGCTGTGCAGCCGCTGCTCAAGGTACGCGGCCTGACC
CGTCTGTACGGTGTGCAGAA

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTHAPMNTAQQRPARDEAYNFAYLDEQTKRMIRRALLKAVAIPGYQVPFGGREMPLPYGWGTGGMQLTAAILGADDVLKV
IDQGADDTTNAVSIRRFFARTAGIATTERTPEATVIQTRHRIPETPLHADQIMVYQVPIPEPLRFIEPSETETRTMHALD
DYGVMHVKLYEDIATFGHIATSYAYPVMVDERYVMDPSPIPKFDNPKLHMSPALMLFGAGREKRLYAVPPYTQVVSLDFE
DHPFEVQRWEQCCAICGSHDSFLDELILDDAGTQSFVCSDTDYCAQRVKQQENDR

Sequences:

>Translated_295_residues
MTHAPMNTAQQRPARDEAYNFAYLDEQTKRMIRRALLKAVAIPGYQVPFGGREMPLPYGWGTGGMQLTAAILGADDVLKV
IDQGADDTTNAVSIRRFFARTAGIATTERTPEATVIQTRHRIPETPLHADQIMVYQVPIPEPLRFIEPSETETRTMHALD
DYGVMHVKLYEDIATFGHIATSYAYPVMVDERYVMDPSPIPKFDNPKLHMSPALMLFGAGREKRLYAVPPYTQVVSLDFE
DHPFEVQRWEQCCAICGSHDSFLDELILDDAGTQSFVCSDTDYCAQRVKQQENDR
>Mature_294_residues
THAPMNTAQQRPARDEAYNFAYLDEQTKRMIRRALLKAVAIPGYQVPFGGREMPLPYGWGTGGMQLTAAILGADDVLKVI
DQGADDTTNAVSIRRFFARTAGIATTERTPEATVIQTRHRIPETPLHADQIMVYQVPIPEPLRFIEPSETETRTMHALDD
YGVMHVKLYEDIATFGHIATSYAYPVMVDERYVMDPSPIPKFDNPKLHMSPALMLFGAGREKRLYAVPPYTQVVSLDFED
HPFEVQRWEQCCAICGSHDSFLDELILDDAGTQSFVCSDTDYCAQRVKQQENDR

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=274, Percent_Identity=78.8321167883212, Blast_Score=466, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 33322; Mature: 33191

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHAPMNTAQQRPARDEAYNFAYLDEQTKRMIRRALLKAVAIPGYQVPFGGREMPLPYGW
CCCCCCCHHHCCCCCHHHCCEEEECHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCC
GTGGMQLTAAILGADDVLKVIDQGADDTTNAVSIRRFFARTAGIATTERTPEATVIQTRH
CCCCCEEHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RIPETPLHADQIMVYQVPIPEPLRFIEPSETETRTMHALDDYGVMHVKLYEDIATFGHIA
CCCCCCCCCCEEEEEECCCCCCHHCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHH
TSYAYPVMVDERYVMDPSPIPKFDNPKLHMSPALMLFGAGREKRLYAVPPYTQVVSLDFE
HHCCCEEEECCEEEECCCCCCCCCCCCEECCCEEEEEECCCCCEEEECCCCCEEEEECCC
DHPFEVQRWEQCCAICGSHDSFLDELILDDAGTQSFVCSDTDYCAQRVKQQENDR
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THAPMNTAQQRPARDEAYNFAYLDEQTKRMIRRALLKAVAIPGYQVPFGGREMPLPYGW
CCCCCCHHHCCCCCHHHCCEEEECHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCC
GTGGMQLTAAILGADDVLKVIDQGADDTTNAVSIRRFFARTAGIATTERTPEATVIQTRH
CCCCCEEHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RIPETPLHADQIMVYQVPIPEPLRFIEPSETETRTMHALDDYGVMHVKLYEDIATFGHIA
CCCCCCCCCCEEEEEECCCCCCHHCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHH
TSYAYPVMVDERYVMDPSPIPKFDNPKLHMSPALMLFGAGREKRLYAVPPYTQVVSLDFE
HHCCCEEEECCEEEECCCCCCCCCCCCEECCCEEEEEECCCCCEEEECCCCCEEEEECCC
DHPFEVQRWEQCCAICGSHDSFLDELILDDAGTQSFVCSDTDYCAQRVKQQENDR
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]