Click here to switch to the map view.

The map label for this gene is nrfA [H]

Identifier: 39995465

GI number: 39995465

Start: 387351

End: 388952

Strand: Reverse

Name: nrfA [H]

Synonym: GSU0357

Alternate gene names: 39995465

Gene position: 388952-387351 (Counterclockwise)

Preceding gene: 39995466

Following gene: 39995446

Centisome position: 10.2

GC content: 59.8

Gene sequence:

>1602_bases
ATGTGGAAGAAACGACTGGCTGTACTGGCGGTGGCCGCGGGAGCGGCAGTTGCCCTGTCGATCCCGGCACTCTCCACGGC
GGCAAAGGGCAAGCCTGCTGCCACGGGTGCCAAGGGTGACGGACGGGAGACATGCTACGGCTGTCACGAGGAGGTCAAGG
CCCTCAAGGAAGGATCAAAGCACGCCCGTCTTGCCTGCGATTCCTGCCACGACAAACTCAAGGAGCACCTGGCCAACTAC
GAGACCAAGCCGGGCACCAATCTGGATCCGGCCAAGTGCGGTTCGTGCCACAAAAATGAATACAGCAGTTTCTTCACGGT
CAACTACGACGCCCAACCGCGCAAGGAGAAAGGGATTCCCACCGGGCGCTCCCCCATGCAGGACAAGCTCCTGGCCGGCC
ACGGTTTCACCTTCGAGCACAACGAACCCCGGGGACACGCCTTCATGGTGGTTGACCAATTCATCGTCGACCGTTTCCAG
GGAGGGCGTTTTCAGTACAAGAAGGGCGCCTGGGGAATGGACGCCACCGGCAAGGCATGGGACATCCTGACCGACACGGG
CAAGAAGCTACCGGAAACGGCCATGGCGGGAAATCCCACCTGTATCCAGTGCAAGACGTCCGATCACATCCTCAAATGGA
AGTTTATGGGGGACAAGGACCCCAAGGCCACGTGGGACCGGAGTTCCGACATCGTTGCAGTGGCCAAGGACACCCAGAAT
CCGGTGGGGTGCATCCACTGTCACGACCCCCATGGCACCCAACCCCGCGTGGTGCGTGACGGCCTGATCCAGGCAATTGA
GAAGGACCCGACCGCCAATATTTTCGCCAAGAACGGCAAGACCGACCTGAAAGTTATCTCTTTCCGCGACGGTTTCCGCA
AGATCGGCGTCATGGAAAAGACCGATTCCCGGCTCATGTGCGCCCAGTGCCACGTTGAGTACAACTGCAACGCGGGCTCC
CAGTGGTCAGACGGCCAGAAAGTGGGCTACGACGACCAGCGCACCAACCACTTCCCCCTCAAAAATGCAAAGGATCTCCT
CGCACACTACAAGAAGCTCGATTTCTACGATTTCAAGCACGCGATCACAGGGGCACGCCTCGTCAAGCTGCAGCACCCGG
AAGCCGAAACCTACGCGGGCAGTGTCCATGACCGGGCCGGCGTCGGCTGCGCCGACTGCCACATGCCGCGCATGAAGGGC
AAAGACGGCAAGATGTTTAAATCCCACGGCGTGATTCGTCCCGCTCACCATGTGAAAGAAGCCTGTCTCGGCTGCCATCC
CAAGAGCACCGTGGAGCAGAAGTCCTACCAGATCGAGGGAACCCGTAACTACATCCGCGGCAAGATGCGCAAGGCGGAAT
ACTGGCTCGGCCAACTGATCGACACCTACGCCGCCGCCAAGCGGATGGGAATCGGTGAAGATGTCCTGGCCAAGGCACGG
GAGAAGCATGAGGAAGCCCATGTACTGTGGGAGTACTGGACCGCTGAAAACAGCGACGGTTTCCACAATCCCGAACTGGC
CCGCGACAGCCTGACCTCCTCCATCGCGGCATCCAAGGCAGGGGTTAAGCTCCTGAACGACGCCATGGAACCCAAGAAGT
AA

Upstream 100 bases:

>100_bases
GCGGCGGCTGCAGACATGTCTGCCCGGCAAAGCCGAACGCGATGAGTATTGAGCCGTTGAACGGCTAGCCGTAACCTATC
ACGAAAGGAGAAATCAACGT

Downstream 100 bases:

>100_bases
CCATTTTCTTCCCGTTGTCCTCCGGCAGAAGGCCCGGTCTGCTCCCGCAGTCCGGGCTTTCTGCCGGAACAGGCCATTTG
TCTCATGCATCGCAACAGCA

Product: cytochrome C

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MWKKRLAVLAVAAGAAVALSIPALSTAAKGKPAATGAKGDGRETCYGCHEEVKALKEGSKHARLACDSCHDKLKEHLANY
ETKPGTNLDPAKCGSCHKNEYSSFFTVNYDAQPRKEKGIPTGRSPMQDKLLAGHGFTFEHNEPRGHAFMVVDQFIVDRFQ
GGRFQYKKGAWGMDATGKAWDILTDTGKKLPETAMAGNPTCIQCKTSDHILKWKFMGDKDPKATWDRSSDIVAVAKDTQN
PVGCIHCHDPHGTQPRVVRDGLIQAIEKDPTANIFAKNGKTDLKVISFRDGFRKIGVMEKTDSRLMCAQCHVEYNCNAGS
QWSDGQKVGYDDQRTNHFPLKNAKDLLAHYKKLDFYDFKHAITGARLVKLQHPEAETYAGSVHDRAGVGCADCHMPRMKG
KDGKMFKSHGVIRPAHHVKEACLGCHPKSTVEQKSYQIEGTRNYIRGKMRKAEYWLGQLIDTYAAAKRMGIGEDVLAKAR
EKHEEAHVLWEYWTAENSDGFHNPELARDSLTSSIAASKAGVKLLNDAMEPKK

Sequences:

>Translated_533_residues
MWKKRLAVLAVAAGAAVALSIPALSTAAKGKPAATGAKGDGRETCYGCHEEVKALKEGSKHARLACDSCHDKLKEHLANY
ETKPGTNLDPAKCGSCHKNEYSSFFTVNYDAQPRKEKGIPTGRSPMQDKLLAGHGFTFEHNEPRGHAFMVVDQFIVDRFQ
GGRFQYKKGAWGMDATGKAWDILTDTGKKLPETAMAGNPTCIQCKTSDHILKWKFMGDKDPKATWDRSSDIVAVAKDTQN
PVGCIHCHDPHGTQPRVVRDGLIQAIEKDPTANIFAKNGKTDLKVISFRDGFRKIGVMEKTDSRLMCAQCHVEYNCNAGS
QWSDGQKVGYDDQRTNHFPLKNAKDLLAHYKKLDFYDFKHAITGARLVKLQHPEAETYAGSVHDRAGVGCADCHMPRMKG
KDGKMFKSHGVIRPAHHVKEACLGCHPKSTVEQKSYQIEGTRNYIRGKMRKAEYWLGQLIDTYAAAKRMGIGEDVLAKAR
EKHEEAHVLWEYWTAENSDGFHNPELARDSLTSSIAASKAGVKLLNDAMEPKK
>Mature_533_residues
MWKKRLAVLAVAAGAAVALSIPALSTAAKGKPAATGAKGDGRETCYGCHEEVKALKEGSKHARLACDSCHDKLKEHLANY
ETKPGTNLDPAKCGSCHKNEYSSFFTVNYDAQPRKEKGIPTGRSPMQDKLLAGHGFTFEHNEPRGHAFMVVDQFIVDRFQ
GGRFQYKKGAWGMDATGKAWDILTDTGKKLPETAMAGNPTCIQCKTSDHILKWKFMGDKDPKATWDRSSDIVAVAKDTQN
PVGCIHCHDPHGTQPRVVRDGLIQAIEKDPTANIFAKNGKTDLKVISFRDGFRKIGVMEKTDSRLMCAQCHVEYNCNAGS
QWSDGQKVGYDDQRTNHFPLKNAKDLLAHYKKLDFYDFKHAITGARLVKLQHPEAETYAGSVHDRAGVGCADCHMPRMKG
KDGKMFKSHGVIRPAHHVKEACLGCHPKSTVEQKSYQIEGTRNYIRGKMRKAEYWLGQLIDTYAAAKRMGIGEDVLAKAR
EKHEEAHVLWEYWTAENSDGFHNPELARDSLTSSIAASKAGVKLLNDAMEPKK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=415, Percent_Identity=23.1325301204819, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 59069; Mature: 59069

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKKRLAVLAVAAGAAVALSIPALSTAAKGKPAATGAKGDGRETCYGCHEEVKALKEGSK
CCHHHEEHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
HARLACDSCHDKLKEHLANYETKPGTNLDPAKCGSCHKNEYSSFFTVNYDAQPRKEKGIP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCHHHCCCC
TGRSPMQDKLLAGHGFTFEHNEPRGHAFMVVDQFIVDRFQGGRFQYKKGAWGMDATGKAW
CCCCCHHHHHHHCCCCEECCCCCCCCEEEEHHHHHHHHHCCCCEEECCCCCCCCCCCCEE
DILTDTGKKLPETAMAGNPTCIQCKTSDHILKWKFMGDKDPKATWDRSSDIVAVAKDTQN
EEEHHCCCCCCCHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCC
PVGCIHCHDPHGTQPRVVRDGLIQAIEKDPTANIFAKNGKTDLKVISFRDGFRKIGVMEK
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEECCCHHHCCCEEC
TDSRLMCAQCHVEYNCNAGSQWSDGQKVGYDDQRTNHFPLKNAKDLLAHYKKLDFYDFKH
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
AITGARLVKLQHPEAETYAGSVHDRAGVGCADCHMPRMKGKDGKMFKSHGVIRPAHHVKE
HHCCEEEEEEECCCCHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCHHHHHH
ACLGCHPKSTVEQKSYQIEGTRNYIRGKMRKAEYWLGQLIDTYAAAKRMGIGEDVLAKAR
HHHCCCCCCHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EKHEEAHVLWEYWTAENSDGFHNPELARDSLTSSIAASKAGVKLLNDAMEPKK
HHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MWKKRLAVLAVAAGAAVALSIPALSTAAKGKPAATGAKGDGRETCYGCHEEVKALKEGSK
CCHHHEEHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
HARLACDSCHDKLKEHLANYETKPGTNLDPAKCGSCHKNEYSSFFTVNYDAQPRKEKGIP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCHHHCCCC
TGRSPMQDKLLAGHGFTFEHNEPRGHAFMVVDQFIVDRFQGGRFQYKKGAWGMDATGKAW
CCCCCHHHHHHHCCCCEECCCCCCCCEEEEHHHHHHHHHCCCCEEECCCCCCCCCCCCEE
DILTDTGKKLPETAMAGNPTCIQCKTSDHILKWKFMGDKDPKATWDRSSDIVAVAKDTQN
EEEHHCCCCCCCHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCC
PVGCIHCHDPHGTQPRVVRDGLIQAIEKDPTANIFAKNGKTDLKVISFRDGFRKIGVMEK
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEECCCHHHCCCEEC
TDSRLMCAQCHVEYNCNAGSQWSDGQKVGYDDQRTNHFPLKNAKDLLAHYKKLDFYDFKH
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
AITGARLVKLQHPEAETYAGSVHDRAGVGCADCHMPRMKGKDGKMFKSHGVIRPAHHVKE
HHCCEEEEEEECCCCHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCCCCHHHHHH
ACLGCHPKSTVEQKSYQIEGTRNYIRGKMRKAEYWLGQLIDTYAAAKRMGIGEDVLAKAR
HHHCCCCCCHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EKHEEAHVLWEYWTAENSDGFHNPELARDSLTSSIAASKAGVKLLNDAMEPKK
HHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA