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The map label for this gene is hemL

Identifier: 39995446

GI number: 39995446

Start: 366273

End: 367556

Strand: Reverse

Name: hemL

Synonym: GSU0337

Alternate gene names: 39995446

Gene position: 367556-366273 (Counterclockwise)

Preceding gene: 39995465

Following gene: 39995445

Centisome position: 9.64

GC content: 57.32

Gene sequence:

>1284_bases
ATGCTCACAGCTCGCTCGAAGGATCTTTTCACGCAAGCCCAGGAATTCATTCCCGGTGGAGTAAACAGTCCCGTACGCGC
CTTCAAGTCAGTGGGCGCCGACCCTCTTTTCATTAAAAAAGCGTTTGGTTGCACAATTACTGACGCCGACAACAACAGTT
ATATCGATTACGTCGGCTCCTGGGGCCCCATGATTCTCGGCCACTGCCACCCCCAGGTTGTCGAAGCCGTAAAGCGGGCC
GTCGAAAGTGGCAGCAGCTTTGGCGCCCCCACGGAGCTGGAAATCACCCTTGCCCGAATGGTCATCGACGCGGTGCCCTC
AATCGAGATGGTTCGTATGGTCAGCTCCGGAACAGAGGCGACCATGAGTGCCATTCGGCTTGCCCGCGGCTACACCGGCC
GGGATAAAATCATCAAATTCTCAGGCTGCTATCATGGCCATGCCGACGCACTCCTGGTGAAGGCTGGCTCAGGGGCTGCC
ACGTTTGGGGTGCCCGATTCGCCGGGAGTTCCCGTCGATGTGGCAAAAAACACGCTCACTGCTCAGTTCAACGATCTTGA
TTCGGTTTCGAAGCTTATTGATGAGAACAAGAATGAGATCGCGTGCATCATTGTCGAGCCTATTGCCGGTAATATGGGCA
CCGTTCCGCCGGGTGAAGGTTTTCTCGAAGGGCTCCGCTCCATCTGCGACAGCGAGGGAATTGTCCTCATTTTCGACGAG
GTAATGACCGGCTTCCGTGTTGCCTATGGTGGAGCTCAGGAACTTTACGGTGTAACTCCCGACATGACCACCCTCGGCAA
GATCATCGGCGGTGGGCTTCCCGTCGGCGCCTTCGGCGGCAAAAAGGACATCATGAAGCTTCTCTCCCCGTCCGGAGGCG
TTTATCAGGCGGGCACGCTGTCGGGCAACCCCCTCGCCATGACAGCTGGTATCGAGACCCTCAAACTGCTTCAGGCAGAC
GGGTTCTATGAGCAACTGGAGCAAACGAGCCGCCGCCTTGCCGAGGGCATCACCGAGGCGGCCAAATCCGCCGGGTACCC
CATCTATCCGACCCGCGTCGGCAGCATGTTCTGCACCTTTTTCACCAGCAACGAGGTCAAGGACTGGCCCACGGCCACAA
CCTGCGACACAAAGGCATTCGCCGCTTTTTTCAGAATGATGCTCGAGAAGGGGATCTATCTGGCTCCTTCGCAGTTTGAA
ACGGCCTTCGTCTCGATCGCCCACACCGAGGTGGAAATCGAGAAGACGATTGTTGCGGCCCGCTCATGCTTTGCCGCTCT
CTAA

Upstream 100 bases:

>100_bases
ACCTCTCAAATCGGCCATTTTACCCCTTGACACCAACACACCAAACATGCTAATAACCCATTAATTTTTCATCACATTTT
CACACCCAAGGAAATCGCCA

Downstream 100 bases:

>100_bases
CGTCAGTCGACCATTTTGATGATTGACAGGCAAGTCCGGGATGTGGTAAGTGATTTTGGTTTGCGGCCCCCCCGGGCAGC
CTCACACTAACCGCCGATGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLTARSKDLFTQAQEFIPGGVNSPVRAFKSVGADPLFIKKAFGCTITDADNNSYIDYVGSWGPMILGHCHPQVVEAVKRA
VESGSSFGAPTELEITLARMVIDAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDKIIKFSGCYHGHADALLVKAGSGAA
TFGVPDSPGVPVDVAKNTLTAQFNDLDSVSKLIDENKNEIACIIVEPIAGNMGTVPPGEGFLEGLRSICDSEGIVLIFDE
VMTGFRVAYGGAQELYGVTPDMTTLGKIIGGGLPVGAFGGKKDIMKLLSPSGGVYQAGTLSGNPLAMTAGIETLKLLQAD
GFYEQLEQTSRRLAEGITEAAKSAGYPIYPTRVGSMFCTFFTSNEVKDWPTATTCDTKAFAAFFRMMLEKGIYLAPSQFE
TAFVSIAHTEVEIEKTIVAARSCFAAL

Sequences:

>Translated_427_residues
MLTARSKDLFTQAQEFIPGGVNSPVRAFKSVGADPLFIKKAFGCTITDADNNSYIDYVGSWGPMILGHCHPQVVEAVKRA
VESGSSFGAPTELEITLARMVIDAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDKIIKFSGCYHGHADALLVKAGSGAA
TFGVPDSPGVPVDVAKNTLTAQFNDLDSVSKLIDENKNEIACIIVEPIAGNMGTVPPGEGFLEGLRSICDSEGIVLIFDE
VMTGFRVAYGGAQELYGVTPDMTTLGKIIGGGLPVGAFGGKKDIMKLLSPSGGVYQAGTLSGNPLAMTAGIETLKLLQAD
GFYEQLEQTSRRLAEGITEAAKSAGYPIYPTRVGSMFCTFFTSNEVKDWPTATTCDTKAFAAFFRMMLEKGIYLAPSQFE
TAFVSIAHTEVEIEKTIVAARSCFAAL
>Mature_427_residues
MLTARSKDLFTQAQEFIPGGVNSPVRAFKSVGADPLFIKKAFGCTITDADNNSYIDYVGSWGPMILGHCHPQVVEAVKRA
VESGSSFGAPTELEITLARMVIDAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDKIIKFSGCYHGHADALLVKAGSGAA
TFGVPDSPGVPVDVAKNTLTAQFNDLDSVSKLIDENKNEIACIIVEPIAGNMGTVPPGEGFLEGLRSICDSEGIVLIFDE
VMTGFRVAYGGAQELYGVTPDMTTLGKIIGGGLPVGAFGGKKDIMKLLSPSGGVYQAGTLSGNPLAMTAGIETLKLLQAD
GFYEQLEQTSRRLAEGITEAAKSAGYPIYPTRVGSMFCTFFTSNEVKDWPTATTCDTKAFAAFFRMMLEKGIYLAPSQFE
TAFVSIAHTEVEIEKTIVAARSCFAAL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=366, Percent_Identity=26.775956284153, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI37574042, Length=333, Percent_Identity=27.9279279279279, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI226442709, Length=353, Percent_Identity=27.7620396600567, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI24119277, Length=311, Percent_Identity=27.0096463022508, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI13994255, Length=334, Percent_Identity=26.6467065868263, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI226442705, Length=332, Percent_Identity=26.5060240963855, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI284507298, Length=274, Percent_Identity=29.1970802919708, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=60.7058823529412, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1789016, Length=350, Percent_Identity=29.4285714285714, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788044, Length=321, Percent_Identity=32.0872274143302, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787560, Length=341, Percent_Identity=30.4985337243402, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789759, Length=309, Percent_Identity=30.0970873786408, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693181, Length=339, Percent_Identity=29.2035398230088, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786991, Length=409, Percent_Identity=25.9168704156479, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=337, Percent_Identity=27.299703264095, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=357, Percent_Identity=26.890756302521, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=335, Percent_Identity=27.1641791044776, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=193, Percent_Identity=31.0880829015544, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17541228, Length=388, Percent_Identity=24.2268041237113, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=332, Percent_Identity=28.0120481927711, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=323, Percent_Identity=26.0061919504644, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357415, Length=309, Percent_Identity=26.537216828479, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_GEOSL (Q74GA9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951397.1
- ProteinModelPortal:   Q74GA9
- SMR:   Q74GA9
- GeneID:   2687321
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0337
- NMPDR:   fig|243231.1.peg.335
- TIGR:   GSU0337
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   GSUL243231:GSU_0337-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45349; Mature: 45349

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTARSKDLFTQAQEFIPGGVNSPVRAFKSVGADPLFIKKAFGCTITDADNNSYIDYVGS
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEHHCCCEEEECCCCCEEEECCC
WGPMILGHCHPQVVEAVKRAVESGSSFGAPTELEITLARMVIDAVPSIEMVRMVSSGTEA
CCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHH
TMSAIRLARGYTGRDKIIKFSGCYHGHADALLVKAGSGAATFGVPDSPGVPVDVAKNTLT
HHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCCCCCCCCHHHCCCCCE
AQFNDLDSVSKLIDENKNEIACIIVEPIAGNMGTVPPGEGFLEGLRSICDSEGIVLIFDE
EHHCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHH
VMTGFRVAYGGAQELYGVTPDMTTLGKIIGGGLPVGAFGGKKDIMKLLSPSGGVYQAGTL
HHHHHEECCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEECCC
SGNPLAMTAGIETLKLLQADGFYEQLEQTSRRLAEGITEAAKSAGYPIYPTRVGSMFCTF
CCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEE
FTSNEVKDWPTATTCDTKAFAAFFRMMLEKGIYLAPSQFETAFVSIAHTEVEIEKTIVAA
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHEEEEEHHHHHHHHHHHHH
RSCFAAL
HHHHHCC
>Mature Secondary Structure
MLTARSKDLFTQAQEFIPGGVNSPVRAFKSVGADPLFIKKAFGCTITDADNNSYIDYVGS
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEHHCCCEEEECCCCCEEEECCC
WGPMILGHCHPQVVEAVKRAVESGSSFGAPTELEITLARMVIDAVPSIEMVRMVSSGTEA
CCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHH
TMSAIRLARGYTGRDKIIKFSGCYHGHADALLVKAGSGAATFGVPDSPGVPVDVAKNTLT
HHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCCCCCCCCHHHCCCCCE
AQFNDLDSVSKLIDENKNEIACIIVEPIAGNMGTVPPGEGFLEGLRSICDSEGIVLIFDE
EHHCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEHHH
VMTGFRVAYGGAQELYGVTPDMTTLGKIIGGGLPVGAFGGKKDIMKLLSPSGGVYQAGTL
HHHHHEECCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEECCC
SGNPLAMTAGIETLKLLQADGFYEQLEQTSRRLAEGITEAAKSAGYPIYPTRVGSMFCTF
CCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEE
FTSNEVKDWPTATTCDTKAFAAFFRMMLEKGIYLAPSQFETAFVSIAHTEVEIEKTIVAA
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHEEEEEHHHHHHHHHHHHH
RSCFAAL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA