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The map label for this gene is pgaB [H]

Identifier: 238892603

GI number: 238892603

Start: 389846

End: 391867

Strand: Reverse

Name: pgaB [H]

Synonym: KP1_0382

Alternate gene names: 238892603

Gene position: 391867-389846 (Counterclockwise)

Preceding gene: 238892604

Following gene: 238892602

Centisome position: 7.47

GC content: 61.62

Gene sequence:

>2022_bases
ATGACCATGCTGAACACGACCCTGCGCAACGGCCTGATGCTTCTCATCTGGCTGTGCCTTATCTTCAGCGTGCGTGCTGA
GGAAGTCCCCTTCCTCGCCCCGCAGCAGCGCCCCCAACTGGAGGCCAATCAACCCTGGCCGGCGGATCGCTTTCTGGTGC
TGGCCTACCATGACGTCGAAGACGATGCCGCCGACCAGCGTTATCTTTCGGTGCGCACCAGCGCACTCAATGAGCAGATC
GCCTGGCTGCTGCATAACGGCTACCATGCGATTAGCGTTCAGGACATTCTCGATGCGCATTATGGCTTAAAGTCGCTGCC
GCCCAAGGCTTTTCTCCTCAGTTTTGACGATGGTTACAGCAGCTTTTACACCCGCGTCTGGCCGCTGCTTAAAGCCTGGA
ACGTCCCGGCGCTGTGGGCGCCGGTGGGCAGCTGGGTGGACACTCCCGCTGGTCAGCCCGTTAATTTTGGCGGCCTGATG
ACGCCGCGCGAGCGCTTCGCTACCTGGGAGATGGTGCGCGAGCTCAGCCGCTCGCCGCTGGTGGAGATCGGCGCTCACAC
CTGGGCCTCGCACTACGGTCTGCCGGCCAATCCGCAGGGCAGCCGCGAGCCTGCCGCCGCCAACCGCGGCTGGGATAAAA
CCACCGGCCGCTATGAAAGCGACGCGCAGTTTACCCGGCGAATGACCGATGACGTGCAGAAAGTGACGGCGAAAATCCAT
GAGGTTGCCGGGAAAACGCCGCGCGCCTGGGTGTGGCCCTACGGCGCCGCCAGCGGCAGCACCCTGACCATCGCCAAACA
GCAAGGGTATCAGCTGGCGTTTACGCTGAACGACGGCCTGGGCAACGTGAAGGATCTGGACAATATTCCACGCCTGCTGA
TCGCCGGTAACCCGTCGCTCAAGGCCTTTGCCAGCGCCGTGACCCAGATTCAGGAAGCCGATCCTGTTCGGGTGATGCAT
GTGGATCTGGACTATGTCTATGACCCCAATCCCGTCCAGCAGGCAAAGAATATCGATAAGCTGGTGCAGCGCGTCTATGA
CATGAAAATCAGCCACGTCTTTCTGCAGGCCTTCTCCGACCCGCAGGGCGACGGCACGGTCAAGTCGCTCTATTTCCCCA
ACCGCTGGCTGCCGATGCGCGCCGACCTGTTTAACTTCGTCTCCTGGCAGTTGCAGACCCGCGGCAATGTGAAGGTTTAT
GCCTGGATGCCGGTGCTGGCCTTCGATCTTGCCTCAGATCTGCCGCGCGTTCAGCGCTGGGATCCGCAAACCGGGAAGGC
GTTACTTGCCCGCCAGCCGTACGTCCGTCTGTCGCCCTGGGATCCCCGGGTTCGCCAGCAGATCACCGACATCTACGAAG
ATCTGGCCCGACACGCCAGCTTCAGCGGGATTCTGTTTCACGACGACGCCGTGCTGACCGATTTTGAAGACGTGAGCCCG
GAGGCGGTCGCCGCCTGGCGGCAGACGGGTCTGGGGCACGATGCCGCCCCGGTGCCCCAACGTCCGCAAGAGCGCCAGGC
ATGGATGCGTTTCAAAAGCCAAACCCTGACCCGCTTCACTCTGGATCTACGCCAGGCAGTGCAGGCCATCCGCGGCCCGC
AGGTGAAAACGGCGCGTAATCTCTTCGCCCTGCCGATCCTGGAGTCGCAAAGCGAAGCCTGGTTTGCGCAGAACCTGGAT
GACTTCCTTGCCGCCTATGACTGGACGGTCCCCATGGCGATGCCGCTGATGGAGTCCGTGCCGATCGACGCCAGCCAGGC
CTGGCTGACGCGGCTGGTGCAGGCCGTCGCCCGCCACCCGGGCGCGCTGAAGAAAACCATCTTTGAACTGCAGGCCAGAG
ACTGGAATCGACGCCAGCAAAACGCCATTCCCGACCAGCAGCTGGCGGACTGGATGCGTTTGCTGCGGCTGAACGGCGTC
AAAAACTACGGCTACTACCCTGACGATTTTATTAACAACCAGCCTGATATCTCTCGTATCAGGCCGCAATTTTCTTCCTG
GTGGTACCCTGACCATGACTGA

Upstream 100 bases:

>100_bases
TCGACACCGGGCTGATGCTGAACTGGGATAAGCGCCCCTACGACAGCAAACGTGAAAACAACCTCGCCATCACTCTTGAT
GCAAATATACGGTTTTAAGG

Downstream 100 bases:

>100_bases
TCGCATTATCGCATTGTGCATTTTATGCCTCGTATTCGGGCTGCCGTTAGGTGTCGCAGCGGTATTTACCGGCGAGCTTA
TCCTCGACTTTGTCTTTTTC

Product: outer membrane N-deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTMLNTTLRNGLMLLIWLCLIFSVRAEEVPFLAPQQRPQLEANQPWPADRFLVLAYHDVEDDAADQRYLSVRTSALNEQI
AWLLHNGYHAISVQDILDAHYGLKSLPPKAFLLSFDDGYSSFYTRVWPLLKAWNVPALWAPVGSWVDTPAGQPVNFGGLM
TPRERFATWEMVRELSRSPLVEIGAHTWASHYGLPANPQGSREPAAANRGWDKTTGRYESDAQFTRRMTDDVQKVTAKIH
EVAGKTPRAWVWPYGAASGSTLTIAKQQGYQLAFTLNDGLGNVKDLDNIPRLLIAGNPSLKAFASAVTQIQEADPVRVMH
VDLDYVYDPNPVQQAKNIDKLVQRVYDMKISHVFLQAFSDPQGDGTVKSLYFPNRWLPMRADLFNFVSWQLQTRGNVKVY
AWMPVLAFDLASDLPRVQRWDPQTGKALLARQPYVRLSPWDPRVRQQITDIYEDLARHASFSGILFHDDAVLTDFEDVSP
EAVAAWRQTGLGHDAAPVPQRPQERQAWMRFKSQTLTRFTLDLRQAVQAIRGPQVKTARNLFALPILESQSEAWFAQNLD
DFLAAYDWTVPMAMPLMESVPIDASQAWLTRLVQAVARHPGALKKTIFELQARDWNRRQQNAIPDQQLADWMRLLRLNGV
KNYGYYPDDFINNQPDISRIRPQFSSWWYPDHD

Sequences:

>Translated_673_residues
MTMLNTTLRNGLMLLIWLCLIFSVRAEEVPFLAPQQRPQLEANQPWPADRFLVLAYHDVEDDAADQRYLSVRTSALNEQI
AWLLHNGYHAISVQDILDAHYGLKSLPPKAFLLSFDDGYSSFYTRVWPLLKAWNVPALWAPVGSWVDTPAGQPVNFGGLM
TPRERFATWEMVRELSRSPLVEIGAHTWASHYGLPANPQGSREPAAANRGWDKTTGRYESDAQFTRRMTDDVQKVTAKIH
EVAGKTPRAWVWPYGAASGSTLTIAKQQGYQLAFTLNDGLGNVKDLDNIPRLLIAGNPSLKAFASAVTQIQEADPVRVMH
VDLDYVYDPNPVQQAKNIDKLVQRVYDMKISHVFLQAFSDPQGDGTVKSLYFPNRWLPMRADLFNFVSWQLQTRGNVKVY
AWMPVLAFDLASDLPRVQRWDPQTGKALLARQPYVRLSPWDPRVRQQITDIYEDLARHASFSGILFHDDAVLTDFEDVSP
EAVAAWRQTGLGHDAAPVPQRPQERQAWMRFKSQTLTRFTLDLRQAVQAIRGPQVKTARNLFALPILESQSEAWFAQNLD
DFLAAYDWTVPMAMPLMESVPIDASQAWLTRLVQAVARHPGALKKTIFELQARDWNRRQQNAIPDQQLADWMRLLRLNGV
KNYGYYPDDFINNQPDISRIRPQFSSWWYPDHD
>Mature_672_residues
TMLNTTLRNGLMLLIWLCLIFSVRAEEVPFLAPQQRPQLEANQPWPADRFLVLAYHDVEDDAADQRYLSVRTSALNEQIA
WLLHNGYHAISVQDILDAHYGLKSLPPKAFLLSFDDGYSSFYTRVWPLLKAWNVPALWAPVGSWVDTPAGQPVNFGGLMT
PRERFATWEMVRELSRSPLVEIGAHTWASHYGLPANPQGSREPAAANRGWDKTTGRYESDAQFTRRMTDDVQKVTAKIHE
VAGKTPRAWVWPYGAASGSTLTIAKQQGYQLAFTLNDGLGNVKDLDNIPRLLIAGNPSLKAFASAVTQIQEADPVRVMHV
DLDYVYDPNPVQQAKNIDKLVQRVYDMKISHVFLQAFSDPQGDGTVKSLYFPNRWLPMRADLFNFVSWQLQTRGNVKVYA
WMPVLAFDLASDLPRVQRWDPQTGKALLARQPYVRLSPWDPRVRQQITDIYEDLARHASFSGILFHDDAVLTDFEDVSPE
AVAAWRQTGLGHDAAPVPQRPQERQAWMRFKSQTLTRFTLDLRQAVQAIRGPQVKTARNLFALPILESQSEAWFAQNLDD
FLAAYDWTVPMAMPLMESVPIDASQAWLTRLVQAVARHPGALKKTIFELQARDWNRRQQNAIPDQQLADWMRLLRLNGVK
NYGYYPDDFINNQPDISRIRPQFSSWWYPDHD

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=671, Percent_Identity=52.9061102831595, Blast_Score=726, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 76822; Mature: 76691

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLNTTLRNGLMLLIWLCLIFSVRAEEVPFLAPQQRPQLEANQPWPADRFLVLAYHDVE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
DDAADQRYLSVRTSALNEQIAWLLHNGYHAISVQDILDAHYGLKSLPPKAFLLSFDDGYS
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHCCCCCEEEEEECCCHH
SFYTRVWPLLKAWNVPALWAPVGSWVDTPAGQPVNFGGLMTPRERFATWEMVRELSRSPL
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VEIGAHTWASHYGLPANPQGSREPAAANRGWDKTTGRYESDAQFTRRMTDDVQKVTAKIH
EEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EVAGKTPRAWVWPYGAASGSTLTIAKQQGYQLAFTLNDGLGNVKDLDNIPRLLIAGNPSL
HHHCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHCCCEEEEECCCCH
KAFASAVTQIQEADPVRVMHVDLDYVYDPNPVQQAKNIDKLVQRVYDMKISHVFLQAFSD
HHHHHHHHHHHCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQGDGTVKSLYFPNRWLPMRADLFNFVSWQLQTRGNVKVYAWMPVLAFDLASDLPRVQRW
CCCCCCEEEEECCCCCCCHHHHHHHHHHHEEECCCCEEEEEEHHHHHHHHHHCCCHHHCC
DPQTGKALLARQPYVRLSPWDPRVRQQITDIYEDLARHASFSGILFHDDAVLTDFEDVSP
CCCCCCCHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHCCH
EAVAAWRQTGLGHDAAPVPQRPQERQAWMRFKSQTLTRFTLDLRQAVQAIRGPQVKTARN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
LFALPILESQSEAWFAQNLDDFLAAYDWTVPMAMPLMESVPIDASQAWLTRLVQAVARHP
EEEEEEECCCCHHHHHHCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
GALKKTIFELQARDWNRRQQNAIPDQQLADWMRLLRLNGVKNYGYYPDDFINNQPDISRI
CHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHH
RPQFSSWWYPDHD
CHHHHCCCCCCCC
>Mature Secondary Structure 
TMLNTTLRNGLMLLIWLCLIFSVRAEEVPFLAPQQRPQLEANQPWPADRFLVLAYHDVE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
DDAADQRYLSVRTSALNEQIAWLLHNGYHAISVQDILDAHYGLKSLPPKAFLLSFDDGYS
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHCCCCCEEEEEECCCHH
SFYTRVWPLLKAWNVPALWAPVGSWVDTPAGQPVNFGGLMTPRERFATWEMVRELSRSPL
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
VEIGAHTWASHYGLPANPQGSREPAAANRGWDKTTGRYESDAQFTRRMTDDVQKVTAKIH
EEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EVAGKTPRAWVWPYGAASGSTLTIAKQQGYQLAFTLNDGLGNVKDLDNIPRLLIAGNPSL
HHHCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHCCCEEEEECCCCH
KAFASAVTQIQEADPVRVMHVDLDYVYDPNPVQQAKNIDKLVQRVYDMKISHVFLQAFSD
HHHHHHHHHHHCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQGDGTVKSLYFPNRWLPMRADLFNFVSWQLQTRGNVKVYAWMPVLAFDLASDLPRVQRW
CCCCCCEEEEECCCCCCCHHHHHHHHHHHEEECCCCEEEEEEHHHHHHHHHHCCCHHHCC
DPQTGKALLARQPYVRLSPWDPRVRQQITDIYEDLARHASFSGILFHDDAVLTDFEDVSP
CCCCCCCHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHCCH
EAVAAWRQTGLGHDAAPVPQRPQERQAWMRFKSQTLTRFTLDLRQAVQAIRGPQVKTARN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
LFALPILESQSEAWFAQNLDDFLAAYDWTVPMAMPLMESVPIDASQAWLTRLVQAVARHP
EEEEEEECCCCHHHHHHCHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
GALKKTIFELQARDWNRRQQNAIPDQQLADWMRLLRLNGVKNYGYYPDDFINNQPDISRI
CHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHHH
RPQFSSWWYPDHD
CHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]