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The map label for this gene is pgaA [H]

Identifier: 238892604

GI number: 238892604

Start: 391870

End: 394359

Strand: Reverse

Name: pgaA [H]

Synonym: KP1_0383

Alternate gene names: 238892604

Gene position: 394359-391870 (Counterclockwise)

Preceding gene: 238892607

Following gene: 238892603

Centisome position: 7.51

GC content: 62.37

Gene sequence:

>2490_bases
GTGTTGTGCAATGGATTCTTGATGAACATATGGCAAATAGAAATGGAAAGATCACCTTATTCAAATACGTTATTGTTTAA
TCGCAACACGAAACTCTCGCTATCAATCGGCTTGCTTTTGCTCTTCCCGGCGCTGGTTCAGGGAGCGGATTCGCTCTATG
ACCAGCAAATCTTGCAAGCGCGCCAAGGCCAGTACGCCCCCTTTCTCAGTTACCTTCAGCAGTACCAGCTGCGGCATGCC
TTAACCCCTTCTCAGGTGGCGGACTGGCTGCAGGTGGCCCTCTGGGCCGGTCAGGACGATGAAGTGGTGAAAGTCTGGCG
ACGCTACCAGGTCTATATGCCGATTCCCGCACGGGGCACCGCCGCCGCGGCGCAGGCCCTGCGCAACCAGAAGCAGTGGC
AAACCTCGCTCACCCTCTGGCAGCAGGCACTCAGCCAGGCCCCTGGCAGCGACGACTATCGTATCGGCTATATCAAAACG
CTCGCCGATGCCCGCAAGGACGGCGAGGCGCTCAGCGAAGCGCGTCGCCTGGTGGCGGAGCAGGCGAGCGTTGCGCACCT
GCAGACGCTCTCCTATGTCTACCTGCGGCTGGGCAAAAGCTGGGACCAGCTGTTGGTGGACACGCAGATCCTCGATCGTG
AACCGCAAAACAAAACCGCGCTGGCCAGCCTGATGGCGACCCTGACCCGCAACCGGATTGATTCCCCTGCTCTCGGCCTG
GCGAATAGCGTCGAACTGACGCCTGCGGAAAAGCGTAACTTGCAGCTGAACGCCGCCGCCGAACTGGTGCGCCTTGCCGA
TACCCCTTCCCGCGAAGAAAAGGCGCGTTACGCGCTGGCCCGCACGGCGCTCACGCAGTATGACGCGATGATCGCCGCCT
GGCACCCCGACCCACAGGCTGCGCCCGACATCATCCGGGCGCGTATTGATCGTCTGGGCGCGCTGTACGCCAGCGCCGAG
TACGCGCAGGTCATCCGCGAATACCAAAGCCTGATCGCCCAGCAGCAGACCGTTCCCGACTGGGCCATCGGCTGGGTCAT
CTCCTCTTTTATCGCCCTTAAGCAAATTGAGCCTGCGCTGACGCTTATCCACCAGCATCCTTCGTGGCTGACAAGCCAAC
AGAATGAGGAACACGAGCTTTTCTACGCTCTGCTGGACACCGGTCAGTACCCGGCGGCGCAACGGTATGTCGCCCGCCTC
ACCAGGAATGCGCCCTACATCCGTCGTCTCTACGGCTCGCCAACGCCGCAGCCGAACGACGACTGGCTCACCGCACAGTC
GCTTAACGTGCACTATTTAGCGGCTACCAACGACCTGCCGCAGGCCGAAGCCCGAATGCAGCGCCTGGCGGCGACGGCCC
CTGGAAACCAGGGCCTGCAGATAGACTATGCCGCCCTTCTGCAGGAGAGAGGATTGCCACGGGCAGCCGAACGCCAGCTA
AAAGCGGCGGAATCTCTGGAACCTGCCAGCCTGCAGCTGGAGCGCCAGCAGGCCTGGGTCGCCCTCGATCTGCAGGAGTG
GCGGCAAATGGATCTGCTGGCTGACGACGTCGTCGCCCGCTCGCCACGGGATCTGAACACCCAGCGCCTGGCCAGAGCGC
GCGAGATCCATCATCTCTCCGAGCTGCGCCTGAGCGTTGGCAAAGGGCTGCATTCCGACAACCCGGTGAGCGGCACGCAC
GACCTCAGTTTTGAGACGGCGATCTACAGCCCGCCGCTAGCCGACAGCTGGCGGTTGTTCGGCGGGCACCGCTTCGCTGA
AGGCAATTTTGAAGAGGGGAAAGGCAGCCGTCGCCAGCTGTTTGCCGGTATTGAATGGCGTCCGCGCGACTACTGGGGCG
AGCTTGAGCTCTCCAGCGTCAATTTTCACGGCGAGAACAAGCCCGGCGTCCGCCTTTCCGCCGCCCATGACGTTAGCGAT
CGCTGGCAGCTGGGCGGCGAACTGGAGCGGATTTCGCAGCAGACGCCGCTGCGCGCGTTACGCAACGGCGTCAGCGCCAA
CCGCGGCGAAGGCTGGCTGCGCTGGTCTCCAAACGAACGCCGGGAGTACCGCTTCAGCGCGGCCGCCAGCCGGTTTACCG
ATCGCAACCGCCGCCAGGAGTACACCCTCTCCGGTAAAGAACGCCTCTGGCAAACCCCATGGTTAACGCTGGATCTGCAG
CCCGGTCTGTCGGCCAGCGCCAATAGCCGCACCGACACCGCCTACTACAGTCCGGCACGGGACCTCGCCGCCACCGCGGC
GCTGGCCGTCGATCACACGCTTTACCAGCGCTATGACACCGTCTGGAGCCAGCAGTTACTCGCCGGCGGCGGCAGCTACT
GGCAAAAAAATCACGCCGCCGGGGCGATCACGGTGCTGGGTTACGGGCAGCGCCTGCGCTGGAACAACGTCGTCGACACC
GGGCTGATGCTGAACTGGGATAAGCGCCCCTACGACAGCAAACGTGAAAACAACCTCGCCATCACTCTTGATGCAAATAT
ACGGTTTTAA

Upstream 100 bases:

>100_bases
AGGAGATATGTTTATTAGTTTGCAATAGGTCATTCACCTGCAGTGCCAGTCAGCTAAATGGGACCATCCTCATCCTTTAA
CTCACCGTCAAAGGACGGTC

Downstream 100 bases:

>100_bases
GGATGACCATGCTGAACACGACCCTGCGCAACGGCCTGATGCTTCTCATCTGGCTGTGCCTTATCTTCAGCGTGCGTGCT
GAGGAAGTCCCCTTCCTCGC

Product: outer membrane protein PgaA

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MLCNGFLMNIWQIEMERSPYSNTLLFNRNTKLSLSIGLLLLFPALVQGADSLYDQQILQARQGQYAPFLSYLQQYQLRHA
LTPSQVADWLQVALWAGQDDEVVKVWRRYQVYMPIPARGTAAAAQALRNQKQWQTSLTLWQQALSQAPGSDDYRIGYIKT
LADARKDGEALSEARRLVAEQASVAHLQTLSYVYLRLGKSWDQLLVDTQILDREPQNKTALASLMATLTRNRIDSPALGL
ANSVELTPAEKRNLQLNAAAELVRLADTPSREEKARYALARTALTQYDAMIAAWHPDPQAAPDIIRARIDRLGALYASAE
YAQVIREYQSLIAQQQTVPDWAIGWVISSFIALKQIEPALTLIHQHPSWLTSQQNEEHELFYALLDTGQYPAAQRYVARL
TRNAPYIRRLYGSPTPQPNDDWLTAQSLNVHYLAATNDLPQAEARMQRLAATAPGNQGLQIDYAALLQERGLPRAAERQL
KAAESLEPASLQLERQQAWVALDLQEWRQMDLLADDVVARSPRDLNTQRLARAREIHHLSELRLSVGKGLHSDNPVSGTH
DLSFETAIYSPPLADSWRLFGGHRFAEGNFEEGKGSRRQLFAGIEWRPRDYWGELELSSVNFHGENKPGVRLSAAHDVSD
RWQLGGELERISQQTPLRALRNGVSANRGEGWLRWSPNERREYRFSAAASRFTDRNRRQEYTLSGKERLWQTPWLTLDLQ
PGLSASANSRTDTAYYSPARDLAATAALAVDHTLYQRYDTVWSQQLLAGGGSYWQKNHAAGAITVLGYGQRLRWNNVVDT
GLMLNWDKRPYDSKRENNLAITLDANIRF

Sequences:

>Translated_829_residues
MLCNGFLMNIWQIEMERSPYSNTLLFNRNTKLSLSIGLLLLFPALVQGADSLYDQQILQARQGQYAPFLSYLQQYQLRHA
LTPSQVADWLQVALWAGQDDEVVKVWRRYQVYMPIPARGTAAAAQALRNQKQWQTSLTLWQQALSQAPGSDDYRIGYIKT
LADARKDGEALSEARRLVAEQASVAHLQTLSYVYLRLGKSWDQLLVDTQILDREPQNKTALASLMATLTRNRIDSPALGL
ANSVELTPAEKRNLQLNAAAELVRLADTPSREEKARYALARTALTQYDAMIAAWHPDPQAAPDIIRARIDRLGALYASAE
YAQVIREYQSLIAQQQTVPDWAIGWVISSFIALKQIEPALTLIHQHPSWLTSQQNEEHELFYALLDTGQYPAAQRYVARL
TRNAPYIRRLYGSPTPQPNDDWLTAQSLNVHYLAATNDLPQAEARMQRLAATAPGNQGLQIDYAALLQERGLPRAAERQL
KAAESLEPASLQLERQQAWVALDLQEWRQMDLLADDVVARSPRDLNTQRLARAREIHHLSELRLSVGKGLHSDNPVSGTH
DLSFETAIYSPPLADSWRLFGGHRFAEGNFEEGKGSRRQLFAGIEWRPRDYWGELELSSVNFHGENKPGVRLSAAHDVSD
RWQLGGELERISQQTPLRALRNGVSANRGEGWLRWSPNERREYRFSAAASRFTDRNRRQEYTLSGKERLWQTPWLTLDLQ
PGLSASANSRTDTAYYSPARDLAATAALAVDHTLYQRYDTVWSQQLLAGGGSYWQKNHAAGAITVLGYGQRLRWNNVVDT
GLMLNWDKRPYDSKRENNLAITLDANIRF
>Mature_829_residues
MLCNGFLMNIWQIEMERSPYSNTLLFNRNTKLSLSIGLLLLFPALVQGADSLYDQQILQARQGQYAPFLSYLQQYQLRHA
LTPSQVADWLQVALWAGQDDEVVKVWRRYQVYMPIPARGTAAAAQALRNQKQWQTSLTLWQQALSQAPGSDDYRIGYIKT
LADARKDGEALSEARRLVAEQASVAHLQTLSYVYLRLGKSWDQLLVDTQILDREPQNKTALASLMATLTRNRIDSPALGL
ANSVELTPAEKRNLQLNAAAELVRLADTPSREEKARYALARTALTQYDAMIAAWHPDPQAAPDIIRARIDRLGALYASAE
YAQVIREYQSLIAQQQTVPDWAIGWVISSFIALKQIEPALTLIHQHPSWLTSQQNEEHELFYALLDTGQYPAAQRYVARL
TRNAPYIRRLYGSPTPQPNDDWLTAQSLNVHYLAATNDLPQAEARMQRLAATAPGNQGLQIDYAALLQERGLPRAAERQL
KAAESLEPASLQLERQQAWVALDLQEWRQMDLLADDVVARSPRDLNTQRLARAREIHHLSELRLSVGKGLHSDNPVSGTH
DLSFETAIYSPPLADSWRLFGGHRFAEGNFEEGKGSRRQLFAGIEWRPRDYWGELELSSVNFHGENKPGVRLSAAHDVSD
RWQLGGELERISQQTPLRALRNGVSANRGEGWLRWSPNERREYRFSAAASRFTDRNRRQEYTLSGKERLWQTPWLTLDLQ
PGLSASANSRTDTAYYSPARDLAATAALAVDHTLYQRYDTVWSQQLLAGGGSYWQKNHAAGAITVLGYGQRLRWNNVVDT
GLMLNWDKRPYDSKRENNLAITLDANIRF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1787261, Length=787, Percent_Identity=42.3125794155019, Blast_Score=621, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 93872; Mature: 93872

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCNGFLMNIWQIEMERSPYSNTLLFNRNTKLSLSIGLLLLFPALVQGADSLYDQQILQA
CCCCHHHHEEEEEEECCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RQGQYAPFLSYLQQYQLRHALTPSQVADWLQVALWAGQDDEVVKVWRRYQVYMPIPARGT
HCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEECCCCCCH
AAAAQALRNQKQWQTSLTLWQQALSQAPGSDDYRIGYIKTLADARKDGEALSEARRLVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHH
QASVAHLQTLSYVYLRLGKSWDQLLVDTQILDREPQNKTALASLMATLTRNRIDSPALGL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ANSVELTPAEKRNLQLNAAAELVRLADTPSREEKARYALARTALTQYDAMIAAWHPDPQA
CCCEEECCCHHCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCC
APDIIRARIDRLGALYASAEYAQVIREYQSLIAQQQTVPDWAIGWVISSFIALKQIEPAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
TLIHQHPSWLTSQQNEEHELFYALLDTGQYPAAQRYVARLTRNAPYIRRLYGSPTPQPND
HHHHCCCCHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
DWLTAQSLNVHYLAATNDLPQAEARMQRLAATAPGNQGLQIDYAALLQERGLPRAAERQL
CEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCCHHHHHHH
KAAESLEPASLQLERQQAWVALDLQEWRQMDLLADDVVARSPRDLNTQRLARAREIHHLS
HHHHCCCCCHHEEEHHHHEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ELRLSVGKGLHSDNPVSGTHDLSFETAIYSPPLADSWRLFGGHRFAEGNFEEGKGSRRQL
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEECCCCCCCCCCCHHHE
FAGIEWRPRDYWGELELSSVNFHGENKPGVRLSAAHDVSDRWQLGGELERISQQTPLRAL
EECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCHHHHCCCHHHHHHHCCHHHHH
RNGVSANRGEGWLRWSPNERREYRFSAAASRFTDRNRRQEYTLSGKERLWQTPWLTLDLQ
HCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCCCEEEECC
PGLSASANSRTDTAYYSPARDLAATAALAVDHTLYQRYDTVWSQQLLAGGGSYWQKNHAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
GAITVLGYGQRLRWNNVVDTGLMLNWDKRPYDSKRENNLAITLDANIRF
CEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MLCNGFLMNIWQIEMERSPYSNTLLFNRNTKLSLSIGLLLLFPALVQGADSLYDQQILQA
CCCCHHHHEEEEEEECCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RQGQYAPFLSYLQQYQLRHALTPSQVADWLQVALWAGQDDEVVKVWRRYQVYMPIPARGT
HCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEECCCCCCH
AAAAQALRNQKQWQTSLTLWQQALSQAPGSDDYRIGYIKTLADARKDGEALSEARRLVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHH
QASVAHLQTLSYVYLRLGKSWDQLLVDTQILDREPQNKTALASLMATLTRNRIDSPALGL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ANSVELTPAEKRNLQLNAAAELVRLADTPSREEKARYALARTALTQYDAMIAAWHPDPQA
CCCEEECCCHHCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCC
APDIIRARIDRLGALYASAEYAQVIREYQSLIAQQQTVPDWAIGWVISSFIALKQIEPAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
TLIHQHPSWLTSQQNEEHELFYALLDTGQYPAAQRYVARLTRNAPYIRRLYGSPTPQPND
HHHHCCCCHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
DWLTAQSLNVHYLAATNDLPQAEARMQRLAATAPGNQGLQIDYAALLQERGLPRAAERQL
CEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCCCHHHHHHH
KAAESLEPASLQLERQQAWVALDLQEWRQMDLLADDVVARSPRDLNTQRLARAREIHHLS
HHHHCCCCCHHEEEHHHHEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ELRLSVGKGLHSDNPVSGTHDLSFETAIYSPPLADSWRLFGGHRFAEGNFEEGKGSRRQL
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEECCCCCCCCCCCHHHE
FAGIEWRPRDYWGELELSSVNFHGENKPGVRLSAAHDVSDRWQLGGELERISQQTPLRAL
EECCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCHHHHCCCHHHHHHHCCHHHHH
RNGVSANRGEGWLRWSPNERREYRFSAAASRFTDRNRRQEYTLSGKERLWQTPWLTLDLQ
HCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCCCEEEECC
PGLSASANSRTDTAYYSPARDLAATAALAVDHTLYQRYDTVWSQQLLAGGGSYWQKNHAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
GAITVLGYGQRLRWNNVVDTGLMLNWDKRPYDSKRENNLAITLDANIRF
CEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]