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The map label for this gene is pgaC [H]

Identifier: 238892602

GI number: 238892602

Start: 388525

End: 389853

Strand: Reverse

Name: pgaC [H]

Synonym: KP1_0381

Alternate gene names: 238892602

Gene position: 389853-388525 (Counterclockwise)

Preceding gene: 238892603

Following gene: 238892601

Centisome position: 7.43

GC content: 60.42

Gene sequence:

>1329_bases
ATGACTGATCGCATTATCGCATTGTGCATTTTATGCCTCGTATTCGGGCTGCCGTTAGGTGTCGCAGCGGTATTTACCGG
CGAGCTTATCCTCGACTTTGTCTTTTTCTGGCCGCTGTTTATGTCAGTGTTGTGGATCACCGGGGGTCTCTACTTCTGGT
TTCAGCTGGAACGTCACTGGCCGTGGGGGGAGGATACCCCGCCGCCGACGCTGCCGGGCAATCCACTGATCTCGATCCTG
ATCCCCTGCTTTAACGAGGAGAAAAACGCCCGGGAAACCATCAGCGCAGCGCTGGCCCAGCGTTACGCCAATCTTGAAGT
GATCGCGATTAACGACGGCTCTTCGGACAACACCGCCCAGGTGCTCCAGCAGCTGGCGCAGGAGGAGCCGAGGCTGCGGG
TGATCCATCTGGCCGCCAATCAGGGAAAAGCGGTGGCGCTCAAAGCGGGCGCCGCCGCCGCCCGCGGCGATCTGCTGGTC
TGCATCGATGGCGACGCCCTGCTGGACCGGGATACCGCGGCCTGGCTGGTCGCGCCGTTGATCCATTATCCGCACGTCGG
GGCGGTCACCGGCAATCCGCGGATCCGCACCCGATCCACGCTGATCGGCCGCATTCAGGTTGGGGAGTTCTCGTCGATCA
TTGGGCTTATCAAACGGACGCAGCGGATCTATGGCCGGGTCTTTACCGTCTCCGGCGTAATCGCCGCCTTCCGGCGCCAG
GCGCTGGCGGACGTCGGCTACTGGAGCCCGGATATGATCACTGAAGATATTGATATCAGCTGGAAGCTTCAGCTCCGCCA
CTGGGACATCTTTTTCGAGCCGCGAGCGCTGTGCTGGATCCTGATGCCGGAGACCCTCAAAGGGTTATGGAAGCAGCGTC
TGCGCTGGGCCCAGGGCGGCGCCGAAGTCTTTCTGGTCAATCTGCGCCGCCTGTTTCACTGGCAGCATCACCGGATGTGG
CCGCTGTTTCTGGAGTATGCCTGTTCCACGCTGTGGGCCTTTGCCTATGCGATCACCATTCTGCTGTTTATCCTCAGCCG
CCTGATGCCGCTGCCTGACAATCTGGCGGTGCATAGCCTGTTTCCACCGGAATTCACCGGCATGCTGCTCGGCCTGATGT
GCCTGCTGCAGTTCGTCGCCAGCCTGTATATCGAGCGGCGCTATGAGAAAAAGGTCGCCAAATCGCTGTTCTGGGTGATC
TGGTTCCCGATGGTCTACTGGATGATCGGCCTGCTGACCACGCTGGTCGCTTTTCCGAAAGTGATGGTGAAACGTCAACG
TTCCCGCGCGCGCTGGGTGAGCCCGGACCGGGGAAAAGGAAGCCTGTGA

Upstream 100 bases:

>100_bases
GGCGTCAAAAACTACGGCTACTACCCTGACGATTTTATTAACAACCAGCCTGATATCTCTCGTATCAGGCCGCAATTTTC
TTCCTGGTGGTACCCTGACC

Downstream 100 bases:

>100_bases
TGAATGAAAACACGCACATCTTAACCGAACATCGGGTGCTGCCCCGGCTGGTCGACGCCGGCTTAACGCTGCTGGCCTGG
CTCGGTTTTCTGTTCTTTCT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTDRIIALCILCLVFGLPLGVAAVFTGELILDFVFFWPLFMSVLWITGGLYFWFQLERHWPWGEDTPPPTLPGNPLISIL
IPCFNEEKNARETISAALAQRYANLEVIAINDGSSDNTAQVLQQLAQEEPRLRVIHLAANQGKAVALKAGAAAARGDLLV
CIDGDALLDRDTAAWLVAPLIHYPHVGAVTGNPRIRTRSTLIGRIQVGEFSSIIGLIKRTQRIYGRVFTVSGVIAAFRRQ
ALADVGYWSPDMITEDIDISWKLQLRHWDIFFEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLVNLRRLFHWQHHRMW
PLFLEYACSTLWAFAYAITILLFILSRLMPLPDNLAVHSLFPPEFTGMLLGLMCLLQFVASLYIERRYEKKVAKSLFWVI
WFPMVYWMIGLLTTLVAFPKVMVKRQRSRARWVSPDRGKGSL

Sequences:

>Translated_442_residues
MTDRIIALCILCLVFGLPLGVAAVFTGELILDFVFFWPLFMSVLWITGGLYFWFQLERHWPWGEDTPPPTLPGNPLISIL
IPCFNEEKNARETISAALAQRYANLEVIAINDGSSDNTAQVLQQLAQEEPRLRVIHLAANQGKAVALKAGAAAARGDLLV
CIDGDALLDRDTAAWLVAPLIHYPHVGAVTGNPRIRTRSTLIGRIQVGEFSSIIGLIKRTQRIYGRVFTVSGVIAAFRRQ
ALADVGYWSPDMITEDIDISWKLQLRHWDIFFEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLVNLRRLFHWQHHRMW
PLFLEYACSTLWAFAYAITILLFILSRLMPLPDNLAVHSLFPPEFTGMLLGLMCLLQFVASLYIERRYEKKVAKSLFWVI
WFPMVYWMIGLLTTLVAFPKVMVKRQRSRARWVSPDRGKGSL
>Mature_441_residues
TDRIIALCILCLVFGLPLGVAAVFTGELILDFVFFWPLFMSVLWITGGLYFWFQLERHWPWGEDTPPPTLPGNPLISILI
PCFNEEKNARETISAALAQRYANLEVIAINDGSSDNTAQVLQQLAQEEPRLRVIHLAANQGKAVALKAGAAAARGDLLVC
IDGDALLDRDTAAWLVAPLIHYPHVGAVTGNPRIRTRSTLIGRIQVGEFSSIIGLIKRTQRIYGRVFTVSGVIAAFRRQA
LADVGYWSPDMITEDIDISWKLQLRHWDIFFEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLVNLRRLFHWQHHRMWP
LFLEYACSTLWAFAYAITILLFILSRLMPLPDNLAVHSLFPPEFTGMLLGLMCLLQFVASLYIERRYEKKVAKSLFWVIW
FPMVYWMIGLLTTLVAFPKVMVKRQRSRARWVSPDRGKGSL

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=438, Percent_Identity=65.7534246575342, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1790044, Length=89, Percent_Identity=33.7078651685393, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786560, Length=208, Percent_Identity=27.8846153846154, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 50434; Mature: 50303

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRIIALCILCLVFGLPLGVAAVFTGELILDFVFFWPLFMSVLWITGGLYFWFQLERHW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECC
PWGEDTPPPTLPGNPLISILIPCFNEEKNARETISAALAQRYANLEVIAINDGSSDNTAQ
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH
VLQQLAQEEPRLRVIHLAANQGKAVALKAGAAAARGDLLVCIDGDALLDRDTAAWLVAPL
HHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHCCCCHHHHHHHHH
IHYPHVGAVTGNPRIRTRSTLIGRIQVGEFSSIIGLIKRTQRIYGRVFTVSGVIAAFRRQ
HHCCCCCEECCCCCEEEHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALADVGYWSPDMITEDIDISWKLQLRHWDIFFEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHCCC
AEVFLVNLRRLFHWQHHRMWPLFLEYACSTLWAFAYAITILLFILSRLMPLPDNLAVHSL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
FPPEFTGMLLGLMCLLQFVASLYIERRYEKKVAKSLFWVIWFPMVYWMIGLLTTLVAFPK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVKRQRSRARWVSPDRGKGSL
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TDRIIALCILCLVFGLPLGVAAVFTGELILDFVFFWPLFMSVLWITGGLYFWFQLERHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECC
PWGEDTPPPTLPGNPLISILIPCFNEEKNARETISAALAQRYANLEVIAINDGSSDNTAQ
CCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH
VLQQLAQEEPRLRVIHLAANQGKAVALKAGAAAARGDLLVCIDGDALLDRDTAAWLVAPL
HHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHCCCCHHHHHHHHH
IHYPHVGAVTGNPRIRTRSTLIGRIQVGEFSSIIGLIKRTQRIYGRVFTVSGVIAAFRRQ
HHCCCCCEECCCCCEEEHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALADVGYWSPDMITEDIDISWKLQLRHWDIFFEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHCCC
AEVFLVNLRRLFHWQHHRMWPLFLEYACSTLWAFAYAITILLFILSRLMPLPDNLAVHSL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
FPPEFTGMLLGLMCLLQFVASLYIERRYEKKVAKSLFWVIWFPMVYWMIGLLTTLVAFPK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVKRQRSRARWVSPDRGKGSL
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]