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The map label for this gene is pbuO [H]

Identifier: 229917686

GI number: 229917686

Start: 1923288

End: 1924577

Strand: Reverse

Name: pbuO [H]

Synonym: EAT1b_1963

Alternate gene names: 229917686

Gene position: 1924577-1923288 (Counterclockwise)

Preceding gene: 229917692

Following gene: 229917684

Centisome position: 64.15

GC content: 51.4

Gene sequence:

>1290_bases
ATGAATATTTTTCAATTGAAACAACATGGTACGAATGTTAAACAGGAAGTGACTGCTGGGATCACAACGTTCGTGACAAT
GGCGTACATCTCTGTCGTCAACCCAGCGATTCTATCTGACGCAGGGATTCCGTTCGCACAAGCATTCACAGCGACAATCA
TTGCCATCCTCATCGGAACAGGCTTGATGGCGTTTTATGCGAACTTACCGATCGCCGTAGCACCCGGGATGGGGCTCAAC
GCATTCTTCGCTTACACGCTCGTCGCTCAAGAACAGATTGCCCCGTCCGCCGCGCTCGGTGTCGTCTTCGTCTCGTCCGT
CTTCTTCTTGATTTTGTCGCTCTCTCCAATTCGGACGAAGTTAATTCAAGCGATTCCGACTTCGTTGAAGCATGCCATCA
CGACCGGAATCGGTCTTTTCATCGCTTCACTCGGATTAAAACTATCAGGTTTGATCGTTGCTGATGAAGCAAACTTGGTG
CGTCTCGGTGACGTGTCTTCTCCAGAAGTACTACTTGTACTCGGTGGTTTGATCGTCTCGGCACTCCTCTTCTCACGAAA
CGTACCCGGTTCTTTACTCATCGGGATGGTACTCACCGGGATTGCGGCTTACTTCATGGACATCTTACAAATCGACGGTG
TCACGGCGATGCCGACGCTTCCCGAGGGACTCATGGTCAACCCTGTGACGGCATTTAGTGATGTCATTCAATACGGTTTG
TTCGGTGCTGTCCTCTCGTTCTTCCTCGTCACGCTTTTTGATACGACAGGTACGCTTGTCGGTGTCGGAAAACAGGCCGG
TCTCTTAAAAGAAGATGGAACAATTGAGAATGGTCACCGTGCACTCTTTGCCGACTCGCTTGCCACAATGGGTGGATCAC
TCGTCGGAACGAGCCCGTCTACGGCATACATCGAGTCTGCTGCCGGTGTCGCGCAAGGTGGACGTACAGGGCTCACAACG
GCCGTCGTCGCTGGATTGTTCGGATTGACACTCTTCTTCAGCCCACTTATCGGCGCCATCTCTGGCGTTGCGGCGATTAC
GGCTCCTGCACTCATCATCGTCGGGGCACTCATGTTGACGAACGTCCGGTTCATCGACTGGAGCGACTTCAGCGAATACT
TCCCAGCGTTCATGACGATTTTGATCATGCCTTTGTCAGGAAGCATCGAACGCGGAATCGCCTTCGGGTTCATCTTGTAC
CCGATTTTCAAAGCAATGAACAAAGAGACGGTTCACCCGCTCATCTACTTGTTCGGCGCACTCTTCATCTTGGAAATGTT
CTTCCTATAA

Upstream 100 bases:

>100_bases
GGAATCGAACATTTTTGTATAGTCATCAAGTTGATAACGTTAATTTAAGCATTTTCCTCAATTAATGTTCGGAAATAGAC
CGAAATAAGGAGTTATATCG

Downstream 100 bases:

>100_bases
ATTAGACAGAAGATTCTCGTCATGTATCGAGGATTCAGTTTGTTGACAAATAACATGTTCGAACTCGTCCTTCCGGCGCC
CGCGCTTTCCTCAGGCAATC

Product: Xanthine/uracil/vitamin C permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNIFQLKQHGTNVKQEVTAGITTFVTMAYISVVNPAILSDAGIPFAQAFTATIIAILIGTGLMAFYANLPIAVAPGMGLN
AFFAYTLVAQEQIAPSAALGVVFVSSVFFLILSLSPIRTKLIQAIPTSLKHAITTGIGLFIASLGLKLSGLIVADEANLV
RLGDVSSPEVLLVLGGLIVSALLFSRNVPGSLLIGMVLTGIAAYFMDILQIDGVTAMPTLPEGLMVNPVTAFSDVIQYGL
FGAVLSFFLVTLFDTTGTLVGVGKQAGLLKEDGTIENGHRALFADSLATMGGSLVGTSPSTAYIESAAGVAQGGRTGLTT
AVVAGLFGLTLFFSPLIGAISGVAAITAPALIIVGALMLTNVRFIDWSDFSEYFPAFMTILIMPLSGSIERGIAFGFILY
PIFKAMNKETVHPLIYLFGALFILEMFFL

Sequences:

>Translated_429_residues
MNIFQLKQHGTNVKQEVTAGITTFVTMAYISVVNPAILSDAGIPFAQAFTATIIAILIGTGLMAFYANLPIAVAPGMGLN
AFFAYTLVAQEQIAPSAALGVVFVSSVFFLILSLSPIRTKLIQAIPTSLKHAITTGIGLFIASLGLKLSGLIVADEANLV
RLGDVSSPEVLLVLGGLIVSALLFSRNVPGSLLIGMVLTGIAAYFMDILQIDGVTAMPTLPEGLMVNPVTAFSDVIQYGL
FGAVLSFFLVTLFDTTGTLVGVGKQAGLLKEDGTIENGHRALFADSLATMGGSLVGTSPSTAYIESAAGVAQGGRTGLTT
AVVAGLFGLTLFFSPLIGAISGVAAITAPALIIVGALMLTNVRFIDWSDFSEYFPAFMTILIMPLSGSIERGIAFGFILY
PIFKAMNKETVHPLIYLFGALFILEMFFL
>Mature_429_residues
MNIFQLKQHGTNVKQEVTAGITTFVTMAYISVVNPAILSDAGIPFAQAFTATIIAILIGTGLMAFYANLPIAVAPGMGLN
AFFAYTLVAQEQIAPSAALGVVFVSSVFFLILSLSPIRTKLIQAIPTSLKHAITTGIGLFIASLGLKLSGLIVADEANLV
RLGDVSSPEVLLVLGGLIVSALLFSRNVPGSLLIGMVLTGIAAYFMDILQIDGVTAMPTLPEGLMVNPVTAFSDVIQYGL
FGAVLSFFLVTLFDTTGTLVGVGKQAGLLKEDGTIENGHRALFADSLATMGGSLVGTSPSTAYIESAAGVAQGGRTGLTT
AVVAGLFGLTLFFSPLIGAISGVAAITAPALIIVGALMLTNVRFIDWSDFSEYFPAFMTILIMPLSGSIERGIAFGFILY
PIFKAMNKETVHPLIYLFGALFILEMFFL

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790150, Length=430, Percent_Identity=41.6279069767442, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI87082309, Length=427, Percent_Identity=42.3887587822014, Blast_Score=304, Evalue=9e-84,
Organism=Escherichia coli, GI48994909, Length=413, Percent_Identity=37.772397094431, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790499, Length=412, Percent_Identity=37.621359223301, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 44928; Mature: 44928

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFQLKQHGTNVKQEVTAGITTFVTMAYISVVNPAILSDAGIPFAQAFTATIIAILIGT
CCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHH
GLMAFYANLPIAVAPGMGLNAFFAYTLVAQEQIAPSAALGVVFVSSVFFLILSLSPIRTK
HHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHH
LIQAIPTSLKHAITTGIGLFIASLGLKLSGLIVADEANLVRLGDVSSPEVLLVLGGLIVS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
ALLFSRNVPGSLLIGMVLTGIAAYFMDILQIDGVTAMPTLPEGLMVNPVTAFSDVIQYGL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEECCHHHHHHHHHHHH
FGAVLSFFLVTLFDTTGTLVGVGKQAGLLKEDGTIENGHRALFADSLATMGGSLVGTSPS
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCC
TAYIESAAGVAQGGRTGLTTAVVAGLFGLTLFFSPLIGAISGVAAITAPALIIVGALMLT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVRFIDWSDFSEYFPAFMTILIMPLSGSIERGIAFGFILYPIFKAMNKETVHPLIYLFGA
CCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LFILEMFFL
HHHHHHHHC
>Mature Secondary Structure
MNIFQLKQHGTNVKQEVTAGITTFVTMAYISVVNPAILSDAGIPFAQAFTATIIAILIGT
CCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHH
GLMAFYANLPIAVAPGMGLNAFFAYTLVAQEQIAPSAALGVVFVSSVFFLILSLSPIRTK
HHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHH
LIQAIPTSLKHAITTGIGLFIASLGLKLSGLIVADEANLVRLGDVSSPEVLLVLGGLIVS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
ALLFSRNVPGSLLIGMVLTGIAAYFMDILQIDGVTAMPTLPEGLMVNPVTAFSDVIQYGL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEECCHHHHHHHHHHHH
FGAVLSFFLVTLFDTTGTLVGVGKQAGLLKEDGTIENGHRALFADSLATMGGSLVGTSPS
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCC
TAYIESAAGVAQGGRTGLTTAVVAGLFGLTLFFSPLIGAISGVAAITAPALIIVGALMLT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVRFIDWSDFSEYFPAFMTILIMPLSGSIERGIAFGFILYPIFKAMNKETVHPLIYLFGA
CCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LFILEMFFL
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]