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The map label for this gene is hrsA [H]

Identifier: 226224744

GI number: 226224744

Start: 2222244

End: 2222717

Strand: Reverse

Name: hrsA [H]

Synonym: Lm4b_02161

Alternate gene names: 226224744

Gene position: 2222717-2222244 (Counterclockwise)

Preceding gene: 226224745

Following gene: 226224743

Centisome position: 76.31

GC content: 36.29

Gene sequence:

>474_bases
ATGGATATCCAAGATTTAGATATTAGTAAAGTAATAAGCCCAGCACTTGTAAATTTAGACTTACAAGCTACAAGTAAATT
AGAAGTCATTGAAGAGTTAACAGATTTACTTGTTGAAACTGGGGCAGTAGCAGACAAAGATGCTTTTATAGCAGATGTAT
TATATCGCGAAGAAGAAGGCAAAACGGGACTAGGAGAAGGAGTCGCTATTCCACACGGGAAGTCGGCGAGCGTTACGAGT
ACATCCATTGCCGTTGGAAGAACTAAAAAACCTGTTGAGTGGGAGTCGCTTGATGATAAACCAGTAAATATTATTATTCT
TTTCGCAGTGAAAAATTCAGATGCTACAACAACTCATATTAAATTACTTCAAAAAGTAGCAATCTTACTTGCGGACGATG
AGGTGATTGGCCAATTTCAAACTGTAAAAACAAAAGAAGATTTAATTAAGTTGTTAGCCAAAAATCAGGATTAG

Upstream 100 bases:

>100_bases
GTACGAAAGAAATCCCATCGTTACTTGTAAGTGCGATGTTTACCGAGAAAGATAAAATAATGGTAAAAAAACTGATGGAA
AGTTTATAGGAGGCGAATTC

Downstream 100 bases:

>100_bases
GAGGAGTTTGGAATGAATATTATTGGAATAGCAGCTTGTACATCGGGAATCGCACATACGTATATTGCTAAAGAAAAACT
AACAAAAGCAGGGACAGCGC

Product: PTS system, fructose-specific enzyme IIA component

Products: NA

Alternate protein names: Putative PTS system EIIABC component; Phosphotransferase enzyme IIA component; PTS system EIIA component; Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MDIQDLDISKVISPALVNLDLQATSKLEVIEELTDLLVETGAVADKDAFIADVLYREEEGKTGLGEGVAIPHGKSASVTS
TSIAVGRTKKPVEWESLDDKPVNIIILFAVKNSDATTTHIKLLQKVAILLADDEVIGQFQTVKTKEDLIKLLAKNQD

Sequences:

>Translated_157_residues
MDIQDLDISKVISPALVNLDLQATSKLEVIEELTDLLVETGAVADKDAFIADVLYREEEGKTGLGEGVAIPHGKSASVTS
TSIAVGRTKKPVEWESLDDKPVNIIILFAVKNSDATTTHIKLLQKVAILLADDEVIGQFQTVKTKEDLIKLLAKNQD
>Mature_157_residues
MDIQDLDISKVISPALVNLDLQATSKLEVIEELTDLLVETGAVADKDAFIADVLYREEEGKTGLGEGVAIPHGKSASVTS
TSIAVGRTKKPVEWESLDDKPVNIIILFAVKNSDATTTHIKLLQKVAILLADDEVIGQFQTVKTKEDLIKLLAKNQD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=135, Percent_Identity=37.037037037037, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI2367327, Length=137, Percent_Identity=27.7372262773723, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788494, Length=134, Percent_Identity=32.089552238806, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17009; Mature: 17009

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQDLDISKVISPALVNLDLQATSKLEVIEELTDLLVETGAVADKDAFIADVLYREEEG
CCCCCCCHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
KTGLGEGVAIPHGKSASVTSTSIAVGRTKKPVEWESLDDKPVNIIILFAVKNSDATTTHI
CCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHH
KLLQKVAILLADDEVIGQFQTVKTKEDLIKLLAKNQD
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDIQDLDISKVISPALVNLDLQATSKLEVIEELTDLLVETGAVADKDAFIADVLYREEEG
CCCCCCCHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
KTGLGEGVAIPHGKSASVTSTSIAVGRTKKPVEWESLDDKPVNIIILFAVKNSDATTTHI
CCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHH
KLLQKVAILLADDEVIGQFQTVKTKEDLIKLLAKNQD
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9063979; 9278503; 8905232 [H]