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The map label for this gene is yusP [H]

Identifier: 222526618

GI number: 222526618

Start: 4216288

End: 4217802

Strand: Reverse

Name: yusP [H]

Synonym: Chy400_3386

Alternate gene names: 222526618

Gene position: 4217802-4216288 (Counterclockwise)

Preceding gene: 222526620

Following gene: 222526615

Centisome position: 80.05

GC content: 58.68

Gene sequence:

>1515_bases
GTGAGTCGCCAACACACTATCCTGATCACGATGGGCGTGATGATGAGCCTGTTTATGGCTTCGATGGAGTCGACCGTTAT
CGCTACAGCCATGCCGGGCATTGTGCGCGATTTGGGTGACATTGAGAGTTTTAGCTGGGTTTTTGCCATTTATATGCTGA
CCAGCACGACCGCCGGCCCTATTTTCGGGCGTCTGTCAGACCTCTTCGGACGCAGGCCGGTCTATATGGTAGCAATCGCT
ATCTTTAGCCTGGGTTCACTCTTGTGTGGGCTGGCAACCACAATGCCTATGCTAATTGCAGCACGCGCACTCCAGGGGTT
GGGCGCCGGCGGACTGCTCCCACTGGCCTTTATCATCGTCGGTGATATTTTCACGCTTGACCAGCGAGCAAAGGTGCAGG
GGCTTTTTTCAGGGGTGTGGGGCATCAGCTCGGTGATTGGCCCACTGATCGGCGGGTTTCTGGTTGATCAGATTTCATGG
CACTGGGTGTTTTGGATTAACCTCCCTCCGGCGGCTATCGCTGCGGCACTGGTCTGGATAGCGTGGCGTGATCCGGTACG
TGCAAACGCCAAACGACCACCCATCGATATTGCCGGTGCATTGTTGTTGAGCGTCGGAATTATTCTACTGCTGCTCGGAC
TCGATCAGCCAACAACGCCCAGCGGCATTCTTATGTTAAGCGCTGCTGTAGCAACGCTGGCCTTGCTGGTGATGGTTGAA
CTGCGGGCACCGGCACCAATTCTCCCGTTTCACCTGTTGCGTGACCGCACATTCGCGATTGCCTGTCTCCATGGCATTCT
GGCCGGTGGTGTGCTGTTCGGCAGCATGAACTACGTGCCACTCTTCGCGCAGGGGGTGCTGGGCACAAGCGCAACCGAGG
CCGGCACCACCCTGACGCCACTGATGCTGGGATGGGTCTTTACCAGCATTATCGGTGGCCGGCTGTTGTTGCGCATGAGC
TATCGCACGCTCACGATTGGCGGGATGGTCATGTTTGTAGCGGGTGCCATCCTCATGGCCCGGCTACGCCCCGATATGAG
TCGGCTTGAACTGGCCATTGCGCTGGGGCTGATGGGGATTGGCATGGGTGCCAGCATTCCCTCGTTTTTGATAGCAGTGC
AGAGCACGGTGGCCCGCAACGTGTTGGGAGCGGCAACCGCGAGTTTGCAATTTTCGCGCACGATTGGCGGGACGATTGGG
GTGGCTGTGCTGGGCACCTTACTGACGAACCGGTTGAGTGCAGAGCTGGCAGCACGCGGCTTACAAGCCGATGCCGTTCC
GGTTGAAGCACTGATCGGCGAGGGAGCAACCGCGATGACCCTTGCCGGCGAGCCACGGCTGGCACTGGCGGCTGCGCTGA
CCGCAGTCTTTGCCGCGCCGCTGGTGTTTGCATTGCTGGCACTGGCAGTGACATTTCTGGCCCCTTCGCACAGCCCTGAT
GATCTGCGCCGTAAGCGTGAACAGCGCGAAGAGGTAACTACGGCGAAAGAAGTTGCGCCACAGCAGGCGGGGTAG

Upstream 100 bases:

>100_bases
CCGGCCAACCAACCCTCTTATAAAGACAATTGGCGGCGATGGAGACGCAAATAACCCCCGTGCAAGCAGGTCAACCACAC
ACGTATAACAAGGAGCCTTT

Downstream 100 bases:

>100_bases
GGGGAGGAGGTAGGAGGTAGGAGATAGGAGGTAGGAGATAGGAGATAGAAGATAGGAGATAGAAGATTAGGAGATAGGAG
ATAAGAGATAGGAAGTTCAA

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSRQHTILITMGVMMSLFMASMESTVIATAMPGIVRDLGDIESFSWVFAIYMLTSTTAGPIFGRLSDLFGRRPVYMVAIA
IFSLGSLLCGLATTMPMLIAARALQGLGAGGLLPLAFIIVGDIFTLDQRAKVQGLFSGVWGISSVIGPLIGGFLVDQISW
HWVFWINLPPAAIAAALVWIAWRDPVRANAKRPPIDIAGALLLSVGIILLLLGLDQPTTPSGILMLSAAVATLALLVMVE
LRAPAPILPFHLLRDRTFAIACLHGILAGGVLFGSMNYVPLFAQGVLGTSATEAGTTLTPLMLGWVFTSIIGGRLLLRMS
YRTLTIGGMVMFVAGAILMARLRPDMSRLELAIALGLMGIGMGASIPSFLIAVQSTVARNVLGAATASLQFSRTIGGTIG
VAVLGTLLTNRLSAELAARGLQADAVPVEALIGEGATAMTLAGEPRLALAAALTAVFAAPLVFALLALAVTFLAPSHSPD
DLRRKREQREEVTTAKEVAPQQAG

Sequences:

>Translated_504_residues
MSRQHTILITMGVMMSLFMASMESTVIATAMPGIVRDLGDIESFSWVFAIYMLTSTTAGPIFGRLSDLFGRRPVYMVAIA
IFSLGSLLCGLATTMPMLIAARALQGLGAGGLLPLAFIIVGDIFTLDQRAKVQGLFSGVWGISSVIGPLIGGFLVDQISW
HWVFWINLPPAAIAAALVWIAWRDPVRANAKRPPIDIAGALLLSVGIILLLLGLDQPTTPSGILMLSAAVATLALLVMVE
LRAPAPILPFHLLRDRTFAIACLHGILAGGVLFGSMNYVPLFAQGVLGTSATEAGTTLTPLMLGWVFTSIIGGRLLLRMS
YRTLTIGGMVMFVAGAILMARLRPDMSRLELAIALGLMGIGMGASIPSFLIAVQSTVARNVLGAATASLQFSRTIGGTIG
VAVLGTLLTNRLSAELAARGLQADAVPVEALIGEGATAMTLAGEPRLALAAALTAVFAAPLVFALLALAVTFLAPSHSPD
DLRRKREQREEVTTAKEVAPQQAG
>Mature_503_residues
SRQHTILITMGVMMSLFMASMESTVIATAMPGIVRDLGDIESFSWVFAIYMLTSTTAGPIFGRLSDLFGRRPVYMVAIAI
FSLGSLLCGLATTMPMLIAARALQGLGAGGLLPLAFIIVGDIFTLDQRAKVQGLFSGVWGISSVIGPLIGGFLVDQISWH
WVFWINLPPAAIAAALVWIAWRDPVRANAKRPPIDIAGALLLSVGIILLLLGLDQPTTPSGILMLSAAVATLALLVMVEL
RAPAPILPFHLLRDRTFAIACLHGILAGGVLFGSMNYVPLFAQGVLGTSATEAGTTLTPLMLGWVFTSIIGGRLLLRMSY
RTLTIGGMVMFVAGAILMARLRPDMSRLELAIALGLMGIGMGASIPSFLIAVQSTVARNVLGAATASLQFSRTIGGTIGV
AVLGTLLTNRLSAELAARGLQADAVPVEALIGEGATAMTLAGEPRLALAAALTAVFAAPLVFALLALAVTFLAPSHSPDD
LRRKREQREEVTTAKEVAPQQAG

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=419, Percent_Identity=27.6849642004773, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790195, Length=398, Percent_Identity=27.1356783919598, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI87081983, Length=403, Percent_Identity=25.3101736972705, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788392, Length=317, Percent_Identity=31.8611987381703, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788710, Length=414, Percent_Identity=26.3285024154589, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790146, Length=136, Percent_Identity=34.5588235294118, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=441, Percent_Identity=25.8503401360544, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=470, Percent_Identity=25.531914893617, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319770, Length=380, Percent_Identity=28.1578947368421, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=31.5436241610738, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=139, Percent_Identity=31.6546762589928, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=383, Percent_Identity=25.5874673629243, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323520, Length=344, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52890; Mature: 52759

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQHTILITMGVMMSLFMASMESTVIATAMPGIVRDLGDIESFSWVFAIYMLTSTTAGP
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IFGRLSDLFGRRPVYMVAIAIFSLGSLLCGLATTMPMLIAARALQGLGAGGLLPLAFIIV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GDIFTLDQRAKVQGLFSGVWGISSVIGPLIGGFLVDQISWHWVFWINLPPAAIAAALVWI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHH
AWRDPVRANAKRPPIDIAGALLLSVGIILLLLGLDQPTTPSGILMLSAAVATLALLVMVE
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LRAPAPILPFHLLRDRTFAIACLHGILAGGVLFGSMNYVPLFAQGVLGTSATEAGTTLTP
HCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHH
LMLGWVFTSIIGGRLLLRMSYRTLTIGGMVMFVAGAILMARLRPDMSRLELAIALGLMGI
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GMGASIPSFLIAVQSTVARNVLGAATASLQFSRTIGGTIGVAVLGTLLTNRLSAELAARG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
LQADAVPVEALIGEGATAMTLAGEPRLALAAALTAVFAAPLVFALLALAVTFLAPSHSPD
CCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DLRRKREQREEVTTAKEVAPQQAG
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRQHTILITMGVMMSLFMASMESTVIATAMPGIVRDLGDIESFSWVFAIYMLTSTTAGP
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
IFGRLSDLFGRRPVYMVAIAIFSLGSLLCGLATTMPMLIAARALQGLGAGGLLPLAFIIV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
GDIFTLDQRAKVQGLFSGVWGISSVIGPLIGGFLVDQISWHWVFWINLPPAAIAAALVWI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHH
AWRDPVRANAKRPPIDIAGALLLSVGIILLLLGLDQPTTPSGILMLSAAVATLALLVMVE
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LRAPAPILPFHLLRDRTFAIACLHGILAGGVLFGSMNYVPLFAQGVLGTSATEAGTTLTP
HCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHH
LMLGWVFTSIIGGRLLLRMSYRTLTIGGMVMFVAGAILMARLRPDMSRLELAIALGLMGI
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
GMGASIPSFLIAVQSTVARNVLGAATASLQFSRTIGGTIGVAVLGTLLTNRLSAELAARG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
LQADAVPVEALIGEGATAMTLAGEPRLALAAALTAVFAAPLVFALLALAVTFLAPSHSPD
CCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DLRRKREQREEVTTAKEVAPQQAG
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]