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The map label for this gene is ligA

Identifier: 220904693

GI number: 220904693

Start: 1704347

End: 1706485

Strand: Reverse

Name: ligA

Synonym: Ddes_1425

Alternate gene names: 220904693

Gene position: 1706485-1704347 (Counterclockwise)

Preceding gene: 220904694

Following gene: 220904692

Centisome position: 59.39

GC content: 60.26

Gene sequence:

>2139_bases
ATGGTACAGAAGAACGAACATCAGGGCGGGCAGTCGCAGCACAGCCTTTTTGCCGCCGGTCCCACGGGCGAAGAACGCCG
GCGCGTCCAGTGGCTTACAGCCGAGCTTGAGCGTCATAATCACCTCTACCATACGCTGGACAGGCCCGAAATCAGTGATG
ACCAGTTTGACGCCCTGTTTCGCGAATTGCAGGATCTGGAAACCCGCTGGCCTGAACTTCGCTCGCTCCATTCACCAACC
TTGCGCGTGGGGGGCGGGCTGCTGGAAGGTCTTGCCAAAAAGGCCCACAGTCTTCAGATGTATGGTCTGGACAACGTATT
TTCAGCCGGGCAGTGGCAGGAATTCGCGGAGCGTATGGCTCGCGCCTGGGGGGGAGATGTGAACGGTCCCCTGCCGGAAA
GCTTCTGGTGTGACCCCAAGCTGGATGGCCTGGCTCTGGAGATCATCTATGCAGACGGCGTGTTGCAAGAAGCCCTTACC
CGGGGAGACGGCGAGGTGGGTGAAGTTGTCACCGACGCTGTGCGTACCATCCGCACCGTACCCCTGCGACTTGCGGGGCC
GGGGCCTTTTCCCGCCAGGCTTGAGGTGCGCGGCGAGGTTGTCATGTATAAAAATGATTTTGCCGTCCTTAATGAAAAAC
AGGAAGCCCTTGGTCTGAAAACCTTTGCCAATCCCAGAAATGCTGCGGCAGGAACACTGCGCCAGCTGGACACTGCCATT
ATCGGTTCACGCCCGTTGCGGTTTCTGGCTTACAGCCTTGGCCAGGCGCTTTGGACGCCCGCGCCCGTCTGTCTGCTGCA
GTCTGAAGTCATGAGTCGGCTCAGGGAATACGGTTTTCTTACTCCGCCGGACGGCAGGCTGTGCAGCAGCGTGGCCGAGG
TGGAGGAGTATGCCCAGTGGGTCAGGGAGCACCGCGCCGCATTCCCGATGGAAATTGACGGGGCTGTGGCCAAGCTGGAC
AATCTTGAGGCCCAGCAGGCGCTTGGTTTTACCGCCCGGGCACCGCGTTTTGCCGTGGCCTTCAAGTTTCCGGCCGAACT
GGCACAGACCCTGCTGAAAGACATTGAGATACAGGTGGGCCGCACGGGGGTGCTTACCCCTGTGGCCATGCTTGAGCCTG
TCTCTGTGGGCGGCGTGATGGTCTCGCGTGCCACCCTGCATAATGAGGATGAAATACGCAATCGCGATGTGCGGGTGGGT
GATACGGTCATGGTGCGCCGCGCAGGCGATGTCATTCCCGAGGTGGTAGGGCCGGTGCTGGAAAAGCGCCCGGAAAACGC
CCGGGAATATGTTTTTCCCCACACTTGTCCGGCATGCGGCCAGCCTGCGTACCGGGAAGAGGGGGAAGCTGCCTGGCGGT
GCGAAAACATGGCCTGTTCCGCTATCCGCCTGCGGGCCATCACGCATTTTGTGTCCAAGGCCGGTCTGGATATCGCGGGC
GTGGGGCAGAAATGGATAGAACAGCTGGTCACCAGTGGCCGGGTGCAGTCTCCTGCTGATCTTTTTACTCTTACGGTGCA
GGAACTGCTGGGCTTTGAGCGCATGGGAGAAGTGCTGGCCCACAAGTTTGTGGACGCGCTTGCCCGCGCCGTACATTCTG
CCACCCTGCCGCGCCTCATCAGCGCTCTGGGTATAAGGCACGTGGGTGAACAGACCGCACGCACCCTGGCTTTGCATTTT
GAAACGCTGGATGAACTGGAAAACGCCGGCGCGGAAACGCTGCTGAGTCTGCCGGATGTGGGACCGGAGGTGGCGTCATC
CATCCATAACTTTTTCAACAGCCCCGCCAACCGAGAACAGCTTGAGCGCTTCAGGGCGCTTGGCCTGTGGCCGCGCGGCG
GCCGGTCTGGCGGCGGTTCTTCCGGCAGCACAGGCGAGGGCGGTTTGGCGTCCGGGCCGCTGGCGGGAAAAAATATTCTT
TTTACCGGAACCCTGAGCATGCCGCGCAGTGAAGCCGAAAAGCTGGCTGAAACCGCCGGTGCCACGCCACTTGGCGGGGT
GAGCAAAAAACTTGACTACCTTGTGGCGGGGGAAAAGGCGGGCAGCAAGCTTGAAAAAGCACAGGCGCTGGGCGTAACGG
TACTCACCGAAGAAGAATTTATGACCATGTTGCGCGAAGCCAAAGCGGCTTCGGAATAA

Upstream 100 bases:

>100_bases
CAACTTGGCTGCGCGTTTACCACATCCCCAATGCTGTCGGTTTGCCAGCTTTTGCAACGATACATCCCCAATTTGACACG
TTCCAGTATAAAGGTCTTTC

Downstream 100 bases:

>100_bases
TTTGAATACAAAGCGAGCAAAGCATGAGTACAGCCATCATCGTGGTAGGGGCCAACGGACGCATGGGCAAAACCATCAGC
CATCTGGCGGCGACGGAGTC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALFRELQDLETRWPELRSLHSPT
LRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMARAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALT
RGDGEVGEVVTDAVRTIRTVPLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI
IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQWVREHRAAFPMEIDGAVAKLD
NLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVGRTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVG
DTVMVRRAGDVIPEVVGPVLEKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG
VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLISALGIRHVGEQTARTLALHF
ETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQLERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNIL
FTGTLSMPRSEAEKLAETAGATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE

Sequences:

>Translated_712_residues
MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALFRELQDLETRWPELRSLHSPT
LRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMARAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALT
RGDGEVGEVVTDAVRTIRTVPLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI
IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQWVREHRAAFPMEIDGAVAKLD
NLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVGRTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVG
DTVMVRRAGDVIPEVVGPVLEKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG
VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLISALGIRHVGEQTARTLALHF
ETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQLERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNIL
FTGTLSMPRSEAEKLAETAGATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE
>Mature_712_residues
MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALFRELQDLETRWPELRSLHSPT
LRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMARAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALT
RGDGEVGEVVTDAVRTIRTVPLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI
IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQWVREHRAAFPMEIDGAVAKLD
NLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVGRTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVG
DTVMVRRAGDVIPEVVGPVLEKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG
VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLISALGIRHVGEQTARTLALHF
ETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQLERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNIL
FTGTLSMPRSEAEKLAETAGATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=676, Percent_Identity=46.4497041420118, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI87082305, Length=286, Percent_Identity=28.6713286713287, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_DESDA (B8J0Q0)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480005.1
- ProteinModelPortal:   B8J0Q0
- GeneID:   7285121
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1425
- HOGENOM:   HBG620317
- ProtClustDB:   CLSK705331
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77625; Mature: 77625

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 140-140 BINDING 138-138 BINDING 161-161 BINDING 199-199 BINDING 318-318 BINDING 342-342

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALF
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHH
RELQDLETRWPELRSLHSPTLRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMA
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCHHHHHHHHH
RAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALTRGDGEVGEVVTDAVRTIRTV
HHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
PLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI
CEEECCCCCCCCEEEECCCEEEEECCHHHHCCHHHHHCHHHCCCCCCHHHHHHHHHHHHH
IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQW
HCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VREHRAAFPMEIDGAVAKLDNLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVG
HHHHHCCCCEECCHHHHHHHCHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCC
RTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVGDTVMVRRAGDVIPEVVGPVL
CCCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH
EKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG
HCCCCCHHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLI
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALGIRHVGEQTARTLALHFETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHH
LERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNILFTGTLSMPRSEAEKLAETAG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
ATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE
CCCCCCHHHHHHHHEECCHHCHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVQKNEHQGGQSQHSLFAAGPTGEERRRVQWLTAELERHNHLYHTLDRPEISDDQFDALF
CCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHH
RELQDLETRWPELRSLHSPTLRVGGGLLEGLAKKAHSLQMYGLDNVFSAGQWQEFAERMA
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHHCCCCHHHHHHHHH
RAWGGDVNGPLPESFWCDPKLDGLALEIIYADGVLQEALTRGDGEVGEVVTDAVRTIRTV
HHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
PLRLAGPGPFPARLEVRGEVVMYKNDFAVLNEKQEALGLKTFANPRNAAAGTLRQLDTAI
CEEECCCCCCCCEEEECCCEEEEECCHHHHCCHHHHHCHHHCCCCCCHHHHHHHHHHHHH
IGSRPLRFLAYSLGQALWTPAPVCLLQSEVMSRLREYGFLTPPDGRLCSSVAEVEEYAQW
HCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VREHRAAFPMEIDGAVAKLDNLEAQQALGFTARAPRFAVAFKFPAELAQTLLKDIEIQVG
HHHHHCCCCEECCHHHHHHHCHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCC
RTGVLTPVAMLEPVSVGGVMVSRATLHNEDEIRNRDVRVGDTVMVRRAGDVIPEVVGPVL
CCCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHH
EKRPENAREYVFPHTCPACGQPAYREEGEAAWRCENMACSAIRLRAITHFVSKAGLDIAG
HCCCCCHHHCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
VGQKWIEQLVTSGRVQSPADLFTLTVQELLGFERMGEVLAHKFVDALARAVHSATLPRLI
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALGIRHVGEQTARTLALHFETLDELENAGAETLLSLPDVGPEVASSIHNFFNSPANREQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHH
LERFRALGLWPRGGRSGGGSSGSTGEGGLASGPLAGKNILFTGTLSMPRSEAEKLAETAG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
ATPLGGVSKKLDYLVAGEKAGSKLEKAQALGVTVLTEEEFMTMLREAKAASE
CCCCCCHHHHHHHHEECCHHCHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA