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The map label for this gene is pckG [H]

Identifier: 218884690

GI number: 218884690

Start: 1281770

End: 1283680

Strand: Direct

Name: pckG [H]

Synonym: DKAM_1379

Alternate gene names: 218884690

Gene position: 1281770-1283680 (Clockwise)

Preceding gene: 218884689

Following gene: 218884693

Centisome position: 93.89

GC content: 45.16

Gene sequence:

>1911_bases
ATGATACGGGGAACATATATGGATACTTACATGGATGATATGCTTACCCGTCTCTCCAGATATGCTAGCAGAGAATCCAT
TGAGAGAATCTCGAGGATAAATAATAGAAAACTCATTGAATGGATTACAGAGGTCGCGGAGCTTCTCGAGCCCAGCTCCA
TATTCGTTAACACTGGTACAAAGGAGGATCTAGAGTATATTAGGAGACGGGCTATTGAGAAAGGAGAGGAGATACCCAGT
AAATACAATCCGCTCCACACGGTTCACTTCGATGGCGTGTTTGATTTAGCAAGGGATAGGGAGAATACTAGGATTCTCAC
TGTGGGTGGTACATCTATCTCGATGATTAATACCAGGGATAGAGAGGAGGGCTTGAGGGAACTTAGGGAGATATATAAGG
GTATAATGAAGGGTAAGGAGATGTATATAGGGTTCTACTGCTTCGGTCCAAAAGATTCATCGTTCACCCTCTACGGTGTT
CAGGTGACTGATTCGGCGTATGTCATGCATAGTGAGAATATACTCTACAGGGTATGCTACGATGTATTTGTTGAGAAAGG
CGAGTCACTAAGATACATGAGGTTCCTCCATAGTACAGGAGAATTAAACGAGTATGGATGGAGTAAGAATGTGTCGAAGA
GAAGGATCTACGTGGATCTGTTGGAGAATATGACATACAGCGTTAACACCCAGTATGCTGGAAACACAGTGGGATTAAAG
AAGCTCTCATTAAGGCTCTGCGTCTACCAGGGGTACAGGGAGGGATGGCTCTGCGAGCATATGTTCATAGTTGGAGTCAA
GGGGCCGGGTGATAGAGTATCATATTTCACGGGCGCATTCCCAGCTGGATGCGGTAAGACCTCTACAGCATTAATAGCTG
ATACCGTGGTCGGGGATGATTTAGCAATAATAAGAAACGTTAATGGTGAGGCAAGGGGAGTGAACCCTGAAGTAGGCATG
TTTGGAATAATTGATGGAGTCAACCCCGTGGATGACCCCGAGATATATAGGATACTTACATCTAGGGATACAGAAGTCAT
ATTTGCAAATGTCCTTCTAACAGAAGACGGTGAAGTATGGTGGAATGGTAAGACTAGTGAGCCCAGAAAGGGAATAAACT
ATGCCGGCGAATGGTGGCCGGGTAAACTGGATGAAAAGGGTAGGCCTGTACCACCATCTCATCCAAACGCCAGGTTCACA
ACCAGTATATTCTACCTGAGTAAGGTGGACCCCAGGATAAATGACCCTATGGGTGTACCGGTTCACGGTATGATCTTTGG
CGGAAGAGACTCAGATACATGGGTGCCTGTGGAGGAGGCGTTTAACTGGGTTCATGGAATAGTCACAAAGGCTGCATCAC
TGGAATCCGAGAGAACCACGGCTGTGCTCGGCAAGGCCGGCGAAAGAGAGTTCAATCCATTTGCTATACTGGACTTCCTC
TCAATATCACCAGGGGACTTCATAAACCTACACTTTAAATTCGGCGAGGAATTAACCAGAGAGCCGAGGATATATGGTGT
CAACTACTTCCTCAGGGATGATAAAGGAAGGTACCTGACTGAGAAAGTGGATAAAAGAGTATGGTTAAAATGGATGGAGC
TCAGGGCAAACAATGACGTGGATGCATTAGAGACTCCTACAGGCTTTATACCGTTGTATGATGACCTGGCTGAATTATTC
AGGAGGGAGCTCAATAAGGAGTTTCCCGTGGAGCTATATGAGAAACTCTTCACGATAAGGGTGGATAAGCACCTCGAGAA
AGCAGAGAGAATATATAGGATATATTATGAGAAAACACCTGAAACACCCAGGTTATTCTTCGACACATTATTAGAGCATA
AGAGAAGGCTTAAGCAGGCACTGGATAAATATGGCTCGTTGATCCCACCGTCCAAGTTAGATAGGAGATGA

Upstream 100 bases:

>100_bases
TATTCATCTCCTAGTCCAGGGGAACACGGGTTACAACCGAAAGCCTCGCCCTTTAGGACAGGGATGAGGTCAGATGTTTT
ATTAATTTTGTAAATATATT

Downstream 100 bases:

>100_bases
AAGGAAAAAGATGGTTCACCGGTTTAAGGCGGGTAATGCGGGCCCGCCGGGATTTGAACCCGGGACCTACGGGTTAAAAG
CCCGCCGCTCTACCAAGCTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MIRGTYMDTYMDDMLTRLSRYASRESIERISRINNRKLIEWITEVAELLEPSSIFVNTGTKEDLEYIRRRAIEKGEEIPS
KYNPLHTVHFDGVFDLARDRENTRILTVGGTSISMINTRDREEGLRELREIYKGIMKGKEMYIGFYCFGPKDSSFTLYGV
QVTDSAYVMHSENILYRVCYDVFVEKGESLRYMRFLHSTGELNEYGWSKNVSKRRIYVDLLENMTYSVNTQYAGNTVGLK
KLSLRLCVYQGYREGWLCEHMFIVGVKGPGDRVSYFTGAFPAGCGKTSTALIADTVVGDDLAIIRNVNGEARGVNPEVGM
FGIIDGVNPVDDPEIYRILTSRDTEVIFANVLLTEDGEVWWNGKTSEPRKGINYAGEWWPGKLDEKGRPVPPSHPNARFT
TSIFYLSKVDPRINDPMGVPVHGMIFGGRDSDTWVPVEEAFNWVHGIVTKAASLESERTTAVLGKAGEREFNPFAILDFL
SISPGDFINLHFKFGEELTREPRIYGVNYFLRDDKGRYLTEKVDKRVWLKWMELRANNDVDALETPTGFIPLYDDLAELF
RRELNKEFPVELYEKLFTIRVDKHLEKAERIYRIYYEKTPETPRLFFDTLLEHKRRLKQALDKYGSLIPPSKLDRR

Sequences:

>Translated_636_residues
MIRGTYMDTYMDDMLTRLSRYASRESIERISRINNRKLIEWITEVAELLEPSSIFVNTGTKEDLEYIRRRAIEKGEEIPS
KYNPLHTVHFDGVFDLARDRENTRILTVGGTSISMINTRDREEGLRELREIYKGIMKGKEMYIGFYCFGPKDSSFTLYGV
QVTDSAYVMHSENILYRVCYDVFVEKGESLRYMRFLHSTGELNEYGWSKNVSKRRIYVDLLENMTYSVNTQYAGNTVGLK
KLSLRLCVYQGYREGWLCEHMFIVGVKGPGDRVSYFTGAFPAGCGKTSTALIADTVVGDDLAIIRNVNGEARGVNPEVGM
FGIIDGVNPVDDPEIYRILTSRDTEVIFANVLLTEDGEVWWNGKTSEPRKGINYAGEWWPGKLDEKGRPVPPSHPNARFT
TSIFYLSKVDPRINDPMGVPVHGMIFGGRDSDTWVPVEEAFNWVHGIVTKAASLESERTTAVLGKAGEREFNPFAILDFL
SISPGDFINLHFKFGEELTREPRIYGVNYFLRDDKGRYLTEKVDKRVWLKWMELRANNDVDALETPTGFIPLYDDLAELF
RRELNKEFPVELYEKLFTIRVDKHLEKAERIYRIYYEKTPETPRLFFDTLLEHKRRLKQALDKYGSLIPPSKLDRR
>Mature_636_residues
MIRGTYMDTYMDDMLTRLSRYASRESIERISRINNRKLIEWITEVAELLEPSSIFVNTGTKEDLEYIRRRAIEKGEEIPS
KYNPLHTVHFDGVFDLARDRENTRILTVGGTSISMINTRDREEGLRELREIYKGIMKGKEMYIGFYCFGPKDSSFTLYGV
QVTDSAYVMHSENILYRVCYDVFVEKGESLRYMRFLHSTGELNEYGWSKNVSKRRIYVDLLENMTYSVNTQYAGNTVGLK
KLSLRLCVYQGYREGWLCEHMFIVGVKGPGDRVSYFTGAFPAGCGKTSTALIADTVVGDDLAIIRNVNGEARGVNPEVGM
FGIIDGVNPVDDPEIYRILTSRDTEVIFANVLLTEDGEVWWNGKTSEPRKGINYAGEWWPGKLDEKGRPVPPSHPNARFT
TSIFYLSKVDPRINDPMGVPVHGMIFGGRDSDTWVPVEEAFNWVHGIVTKAASLESERTTAVLGKAGEREFNPFAILDFL
SISPGDFINLHFKFGEELTREPRIYGVNYFLRDDKGRYLTEKVDKRVWLKWMELRANNDVDALETPTGFIPLYDDLAELF
RRELNKEFPVELYEKLFTIRVDKHLEKAERIYRIYYEKTPETPRLFFDTLLEHKRRLKQALDKYGSLIPPSKLDRR

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=544, Percent_Identity=31.6176470588235, Blast_Score=230, Evalue=4e-60,
Organism=Homo sapiens, GI187281517, Length=591, Percent_Identity=29.4416243654822, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI66346723, Length=386, Percent_Identity=30.0518134715026, Blast_Score=149, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17555492, Length=553, Percent_Identity=28.3905967450271, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI32564411, Length=556, Percent_Identity=28.0575539568345, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71989658, Length=595, Percent_Identity=27.2268907563025, Blast_Score=205, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI71989645, Length=595, Percent_Identity=27.2268907563025, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI71989653, Length=583, Percent_Identity=26.7581475128645, Blast_Score=196, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI32564407, Length=484, Percent_Identity=29.1322314049587, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71989649, Length=481, Percent_Identity=28.8981288981289, Blast_Score=181, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI32564409, Length=307, Percent_Identity=29.3159609120521, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24655082, Length=603, Percent_Identity=29.5190713101161, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24655085, Length=541, Percent_Identity=29.0203327171904, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI161077226, Length=545, Percent_Identity=30.8256880733945, Blast_Score=224, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655088, Length=444, Percent_Identity=31.7567567567568, Blast_Score=218, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 73418; Mature: 73418

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRGTYMDTYMDDMLTRLSRYASRESIERISRINNRKLIEWITEVAELLEPSSIFVNTGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCC
KEDLEYIRRRAIEKGEEIPSKYNPLHTVHFDGVFDLARDRENTRILTVGGTSISMINTRD
HHHHHHHHHHHHHHHHHCCHHCCCEEEEEECCHHHHHCCCCCCEEEEECCCEEEEECCCC
REEGLRELREIYKGIMKGKEMYIGFYCFGPKDSSFTLYGVQVTDSAYVMHSENILYRVCY
HHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEEEECCEEEEECCCHHHHHHH
DVFVEKGESLRYMRFLHSTGELNEYGWSKNVSKRRIYVDLLENMTYSVNTQYAGNTVGLK
HHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCHH
KLSLRLCVYQGYREGWLCEHMFIVGVKGPGDRVSYFTGAFPAGCGKTSTALIADTVVGDD
HHHHHHEEHHCCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCCCCCCCEEEHHHCCCC
LAIIRNVNGEARGVNPEVGMFGIIDGVNPVDDPEIYRILTSRDTEVIFANVLLTEDGEVW
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEEECCCCCEEEEEEEEEECCCCEE
WNGKTSEPRKGINYAGEWWPGKLDEKGRPVPPSHPNARFTTSIFYLSKVDPRINDPMGVP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCC
VHGMIFGGRDSDTWVPVEEAFNWVHGIVTKAASLESERTTAVLGKAGEREFNPFAILDFL
EEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEH
SISPGDFINLHFKFGEELTREPRIYGVNYFLRDDKGRYLTEKVDKRVWLKWMELRANNDV
CCCCCCEEEEEEECCHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
DALETPTGFIPLYDDLAELFRRELNKEFPVELYEKLFTIRVDKHLEKAERIYRIYYEKTP
CCCCCCCCCEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETPRLFFDTLLEHKRRLKQALDKYGSLIPPSKLDRR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MIRGTYMDTYMDDMLTRLSRYASRESIERISRINNRKLIEWITEVAELLEPSSIFVNTGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCC
KEDLEYIRRRAIEKGEEIPSKYNPLHTVHFDGVFDLARDRENTRILTVGGTSISMINTRD
HHHHHHHHHHHHHHHHHCCHHCCCEEEEEECCHHHHHCCCCCCEEEEECCCEEEEECCCC
REEGLRELREIYKGIMKGKEMYIGFYCFGPKDSSFTLYGVQVTDSAYVMHSENILYRVCY
HHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEEEECCEEEEECCCHHHHHHH
DVFVEKGESLRYMRFLHSTGELNEYGWSKNVSKRRIYVDLLENMTYSVNTQYAGNTVGLK
HHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCHH
KLSLRLCVYQGYREGWLCEHMFIVGVKGPGDRVSYFTGAFPAGCGKTSTALIADTVVGDD
HHHHHHEEHHCCCCCCEEEEEEEEEECCCCCCCEEECCCCCCCCCCCCCCEEEHHHCCCC
LAIIRNVNGEARGVNPEVGMFGIIDGVNPVDDPEIYRILTSRDTEVIFANVLLTEDGEVW
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHEEEECCCCCEEEEEEEEEECCCCEE
WNGKTSEPRKGINYAGEWWPGKLDEKGRPVPPSHPNARFTTSIFYLSKVDPRINDPMGVP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCC
VHGMIFGGRDSDTWVPVEEAFNWVHGIVTKAASLESERTTAVLGKAGEREFNPFAILDFL
EEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEH
SISPGDFINLHFKFGEELTREPRIYGVNYFLRDDKGRYLTEKVDKRVWLKWMELRANNDV
CCCCCCEEEEEEECCHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
DALETPTGFIPLYDDLAELFRRELNKEFPVELYEKLFTIRVDKHLEKAERIYRIYYEKTP
CCCCCCCCCEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETPRLFFDTLLEHKRRLKQALDKYGSLIPPSKLDRR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA