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The map label for this gene is fusA [H]

Identifier: 209694218

GI number: 209694218

Start: 691624

End: 693708

Strand: Direct

Name: fusA [H]

Synonym: VSAL_I0627

Alternate gene names: 209694218

Gene position: 691624-693708 (Clockwise)

Preceding gene: 209694217

Following gene: 209694224

Centisome position: 20.8

GC content: 43.31

Gene sequence:

>2085_bases
ATGACTGATTTATCTAAATACAGAAATATTGGTATTTTCGCGCACGTTGATGCGGGCAAAACTACCACAACTGAGCGTAT
CCTAAAACTTACTGGTCAGATCCACAAAACTGGTGAAGTACATGATGGCGAATCTACGACTGACTTCATGGAACAGGAAG
CTGAGCGCGGAATTACTATCCAGTCAGCAGCTGTAAGTTGTTTCTGGAAAGATCACCGTTTTAACGTTATCGATACTCCT
GGACACGTTGACTTCACAGTTGAAGTTTACCGTTCACTAAAAGTACTTGACGGTGGTATCGGTGTATTCTGTGGTTCTGG
TGGTGTTGAACCTCAATCAGAAACTAACTGGCGTTACGCGAACGAATCAGAAGTTGCACGTATTATCTTCGTTAACAAAC
TAGACCGTATGGGTGCTGACTTCTTCCGTGTTATTAAGCAGACTGAAACAGTACTAGGTGCTAACCCACTAATCATGGTA
CTACCTATCGGTATCGAAGATGATTTCGTTGGTGTTGTTGACCTTTTAACTCGCAAAGCTTTCGTTTGGGATGACACTGG
TCTTCCTGAAAACTACGAAATCACTGATATCCCAGCGGATATGGTTGATCAAGTAGAAGAATACCGTGAGATGCTAGTTG
AAACTGCTGTAGAGCAAGACGACGACCTAATGGAAGCTTACATGGAAGGCGAAGAGCCAACTATTGAGCAACTAAAAATG
TGTATCCGTAAGGGTACTCGCACAATGGATTTCTTCCCTGCTTACTGTGGTTCTGCGTTTAAGAACAAAGGTATGCAACT
AATCCTTGATGCTGTTGTTGATTACCTACCTTCTCCAACAGAAGTTGATCCTCAACCTCTAATGGATGAGAACGGCGAAG
AAAATGGCGAGCACGCTATTGTTTCTGTTGATGAAACATTCAAAGCACTAGCATTCAAAATCATGGATGACCGTTTTGGT
GCCCTAACTTTCGTTCGTATTTACTCTGGTAAATTGAACAAAGGCGACACAATTCTTAACTCATTCACAGGTAAAACTGA
GCGTGTTGGCCGTATGGTTGAGATGCAAGCGAATGACCGTAATGAATTAACTTACGCACAAGCTGGTGACATCATCGCTA
TCGTGGGTATGAAAAACGTGCAAACTGGTCACACTCTTTGTGATCCTAAGCACGAAGTAACACTTGAGCCAATGGTTTTC
CCTGTACCAGTAATCTCAATCTCAGTAACGCCTAAAGATAAAGGCAGTACTGAGAAAATGGGTATTGCTATCGGTAAAAT
GGTTGCAGAAGATCCATCTTTCCAAGTTGAAACTGACGAAGAAACTGGCGAAACAATCCTTAAAGGTATGGGTGAACTTC
ACCTAGACATTAAAGTTGATATCCTTCGTCGTACATACGGTGTAGATCTTGCTGTTGGTCAACCTCAGGTAGCTTACCGT
GAAACTATCACTCAACTTGTTGAAGATAGCTACACGCATAAGAAACAATCTGGTGGTTCTGGTCAGTTCGGTAAGATCGA
CTACATCATGAAACCAGGCGAAGTTGGTTCAGGCTTCTCATTCAAGTCTTCTGTTGTGGGCGGTAACGTTCCTAAAGAAT
TCTGGCCTGCAATCGAGAAAGGCTTCAAAGGCATGATGGATACTGGTGTTCTTGCTGGTTTCCCTGTTCTTGATGTTGAA
ATCGAACTTCTTGACGGTGGCTTCCACGCAGTCGATTCATCTGCAGTAGCATTTGAAATCGCAGCGAAAGGCGCATTCCG
TCAATCTATGCCTAAAGCTGGTGCACAACTTCTAGAACCAATCATGGCTGTAGACGTGTTCACTCCAGATGATCACGTTG
GTGATGTAATCGGTGACCTTAACCGTCGTCGTGGCATGATCAAAGACCAAATGGCTGGCGTAACTGGTGTTCGTGTTAAA
GCTGACATTCCTCTTTCAGAAATGTTTGGTTACATCGGTTCTCTACGTACAATGACTTCTGGTCGTGGTCAGTTCTCTAT
GGAGTTCTCACATTACGCACCTTGTCCAATGAACGTTGCTGAGCAAGTAATCGTTGCAGTTAAAGAAGCTAACGCTAAGA
AGTAA

Upstream 100 bases:

>100_bases
TGGGGCTAAATTCTATCCAAAAATAGAACAAAGCATATACCAATTGACAGTTTATGTTATACTCTGCGCGCATTTTCTAT
CCTATTAATAGAGTAAAACA

Downstream 100 bases:

>100_bases
TTCTGGCTAACGCTCTTTAATTAAAAAAGCCCCGTAAGTGAAAACTTACGGGGCTTTTTATTTTTAAGCGATTAAATAAC
GTAGATACAGAGACACTCTC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 2 [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGQIHKTGEVHDGESTTDFMEQEAERGITIQSAAVSCFWKDHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARIIFVNKLDRMGADFFRVIKQTETVLGANPLIMV
LPIGIEDDFVGVVDLLTRKAFVWDDTGLPENYEITDIPADMVDQVEEYREMLVETAVEQDDDLMEAYMEGEEPTIEQLKM
CIRKGTRTMDFFPAYCGSAFKNKGMQLILDAVVDYLPSPTEVDPQPLMDENGEENGEHAIVSVDETFKALAFKIMDDRFG
ALTFVRIYSGKLNKGDTILNSFTGKTERVGRMVEMQANDRNELTYAQAGDIIAIVGMKNVQTGHTLCDPKHEVTLEPMVF
PVPVISISVTPKDKGSTEKMGIAIGKMVAEDPSFQVETDEETGETILKGMGELHLDIKVDILRRTYGVDLAVGQPQVAYR
ETITQLVEDSYTHKKQSGGSGQFGKIDYIMKPGEVGSGFSFKSSVVGGNVPKEFWPAIEKGFKGMMDTGVLAGFPVLDVE
IELLDGGFHAVDSSAVAFEIAAKGAFRQSMPKAGAQLLEPIMAVDVFTPDDHVGDVIGDLNRRRGMIKDQMAGVTGVRVK
ADIPLSEMFGYIGSLRTMTSGRGQFSMEFSHYAPCPMNVAEQVIVAVKEANAKK

Sequences:

>Translated_694_residues
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGQIHKTGEVHDGESTTDFMEQEAERGITIQSAAVSCFWKDHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARIIFVNKLDRMGADFFRVIKQTETVLGANPLIMV
LPIGIEDDFVGVVDLLTRKAFVWDDTGLPENYEITDIPADMVDQVEEYREMLVETAVEQDDDLMEAYMEGEEPTIEQLKM
CIRKGTRTMDFFPAYCGSAFKNKGMQLILDAVVDYLPSPTEVDPQPLMDENGEENGEHAIVSVDETFKALAFKIMDDRFG
ALTFVRIYSGKLNKGDTILNSFTGKTERVGRMVEMQANDRNELTYAQAGDIIAIVGMKNVQTGHTLCDPKHEVTLEPMVF
PVPVISISVTPKDKGSTEKMGIAIGKMVAEDPSFQVETDEETGETILKGMGELHLDIKVDILRRTYGVDLAVGQPQVAYR
ETITQLVEDSYTHKKQSGGSGQFGKIDYIMKPGEVGSGFSFKSSVVGGNVPKEFWPAIEKGFKGMMDTGVLAGFPVLDVE
IELLDGGFHAVDSSAVAFEIAAKGAFRQSMPKAGAQLLEPIMAVDVFTPDDHVGDVIGDLNRRRGMIKDQMAGVTGVRVK
ADIPLSEMFGYIGSLRTMTSGRGQFSMEFSHYAPCPMNVAEQVIVAVKEANAKK
>Mature_693_residues
TDLSKYRNIGIFAHVDAGKTTTTERILKLTGQIHKTGEVHDGESTTDFMEQEAERGITIQSAAVSCFWKDHRFNVIDTPG
HVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARIIFVNKLDRMGADFFRVIKQTETVLGANPLIMVL
PIGIEDDFVGVVDLLTRKAFVWDDTGLPENYEITDIPADMVDQVEEYREMLVETAVEQDDDLMEAYMEGEEPTIEQLKMC
IRKGTRTMDFFPAYCGSAFKNKGMQLILDAVVDYLPSPTEVDPQPLMDENGEENGEHAIVSVDETFKALAFKIMDDRFGA
LTFVRIYSGKLNKGDTILNSFTGKTERVGRMVEMQANDRNELTYAQAGDIIAIVGMKNVQTGHTLCDPKHEVTLEPMVFP
VPVISISVTPKDKGSTEKMGIAIGKMVAEDPSFQVETDEETGETILKGMGELHLDIKVDILRRTYGVDLAVGQPQVAYRE
TITQLVEDSYTHKKQSGGSGQFGKIDYIMKPGEVGSGFSFKSSVVGGNVPKEFWPAIEKGFKGMMDTGVLAGFPVLDVEI
ELLDGGFHAVDSSAVAFEIAAKGAFRQSMPKAGAQLLEPIMAVDVFTPDDHVGDVIGDLNRRRGMIKDQMAGVTGVRVKA
DIPLSEMFGYIGSLRTMTSGRGQFSMEFSHYAPCPMNVAEQVIVAVKEANAKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=700, Percent_Identity=42.4285714285714, Blast_Score=545, Evalue=1e-155,
Organism=Homo sapiens, GI19923640, Length=711, Percent_Identity=36.1462728551336, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI25306283, Length=396, Percent_Identity=41.4141414141414, Blast_Score=270, Evalue=4e-72,
Organism=Homo sapiens, GI25306287, Length=290, Percent_Identity=45.5172413793103, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=39.622641509434, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=34.5323741007194, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4503483, Length=153, Percent_Identity=36.6013071895425, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI310132016, Length=122, Percent_Identity=36.0655737704918, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI310110807, Length=122, Percent_Identity=36.0655737704918, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI310123363, Length=122, Percent_Identity=36.0655737704918, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1789738, Length=697, Percent_Identity=53.6585365853659, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=503, Percent_Identity=24.0556660039761, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788922, Length=155, Percent_Identity=36.1290322580645, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=38.2352941176471, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=695, Percent_Identity=39.136690647482, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17556745, Length=707, Percent_Identity=29.1371994342291, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17557151, Length=151, Percent_Identity=38.4105960264901, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=159, Percent_Identity=36.4779874213836, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17552882, Length=135, Percent_Identity=32.5925925925926, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=31.3432835820896, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=31.3432835820896, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=698, Percent_Identity=41.6905444126075, Blast_Score=545, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6322359, Length=773, Percent_Identity=31.8240620957309, Blast_Score=344, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6324707, Length=549, Percent_Identity=22.7686703096539, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=549, Percent_Identity=22.7686703096539, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=175, Percent_Identity=36.5714285714286, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=180, Percent_Identity=30, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=135, Percent_Identity=34.8148148148148, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=40.1721664275466, Blast_Score=532, Evalue=1e-151,
Organism=Drosophila melanogaster, GI221458488, Length=739, Percent_Identity=32.2056833558863, Blast_Score=348, Evalue=8e-96,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=36.7741935483871, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=171, Percent_Identity=32.7485380116959, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=37.7483443708609, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=37.7483443708609, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=37.7483443708609, Blast_Score=86, Evalue=9e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76398; Mature: 76267

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGQIHKTGEVHDGESTTDFMEQEAERGITI
CCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEE
QSAAVSCFWKDHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EHHHEEEEEECCCCEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVARIIFVNKLDRMGADFFRVIKQTETVLGANPLIMVLPIGIEDDFVGVVDLLTRKA
CCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCE
FVWDDTGLPENYEITDIPADMVDQVEEYREMLVETAVEQDDDLMEAYMEGEEPTIEQLKM
EEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
CIRKGTRTMDFFPAYCGSAFKNKGMQLILDAVVDYLPSPTEVDPQPLMDENGEENGEHAI
HHHCCCCCHHHHHHHHCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
VSVDETFKALAFKIMDDRFGALTFVRIYSGKLNKGDTILNSFTGKTERVGRMVEMQANDR
EEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCHHHHCCEEEECCCCC
NELTYAQAGDIIAIVGMKNVQTGHTLCDPKHEVTLEPMVFPVPVISISVTPKDKGSTEKM
CCEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCEEEECEEEEEEECCCCCCCCHHH
GIAIGKMVAEDPSFQVETDEETGETILKGMGELHLDIKVDILRRTYGVDLAVGQPQVAYR
HHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEEEEEEHHHHCCEEECCCCCHHHH
ETITQLVEDSYTHKKQSGGSGQFGKIDYIMKPGEVGSGFSFKSSVVGGNVPKEFWPAIEK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GFKGMMDTGVLAGFPVLDVEIELLDGGFHAVDSSAVAFEIAAKGAFRQSMPKAGAQLLEP
HHCCHHHCCHHCCCCEEEEEEEEECCCCEECCCCCEEEEEHHCCHHHHHCCHHHHHHHHH
IMAVDVFTPDDHVGDVIGDLNRRRGMIKDQMAGVTGVRVKADIPLSEMFGYIGSLRTMTS
HHHEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCC
GRGQFSMEFSHYAPCPMNVAEQVIVAVKEANAKK
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDLSKYRNIGIFAHVDAGKTTTTERILKLTGQIHKTGEVHDGESTTDFMEQEAERGITI
CCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEE
QSAAVSCFWKDHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EHHHEEEEEECCCCEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVARIIFVNKLDRMGADFFRVIKQTETVLGANPLIMVLPIGIEDDFVGVVDLLTRKA
CCCCEEEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCE
FVWDDTGLPENYEITDIPADMVDQVEEYREMLVETAVEQDDDLMEAYMEGEEPTIEQLKM
EEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
CIRKGTRTMDFFPAYCGSAFKNKGMQLILDAVVDYLPSPTEVDPQPLMDENGEENGEHAI
HHHCCCCCHHHHHHHHCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
VSVDETFKALAFKIMDDRFGALTFVRIYSGKLNKGDTILNSFTGKTERVGRMVEMQANDR
EEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCHHHHCCEEEECCCCC
NELTYAQAGDIIAIVGMKNVQTGHTLCDPKHEVTLEPMVFPVPVISISVTPKDKGSTEKM
CCEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCEEEECEEEEEEECCCCCCCCHHH
GIAIGKMVAEDPSFQVETDEETGETILKGMGELHLDIKVDILRRTYGVDLAVGQPQVAYR
HHHHHHHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEEEEEEHHHHCCEEECCCCCHHHH
ETITQLVEDSYTHKKQSGGSGQFGKIDYIMKPGEVGSGFSFKSSVVGGNVPKEFWPAIEK
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
GFKGMMDTGVLAGFPVLDVEIELLDGGFHAVDSSAVAFEIAAKGAFRQSMPKAGAQLLEP
HHCCHHHCCHHCCCCEEEEEEEEECCCCEECCCCCEEEEEHHCCHHHHHCCHHHHHHHHH
IMAVDVFTPDDHVGDVIGDLNRRRGMIKDQMAGVTGVRVKADIPLSEMFGYIGSLRTMTS
HHHEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCC
GRGQFSMEFSHYAPCPMNVAEQVIVAVKEANAKK
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA