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The map label for this gene is radA [H]

Identifier: 209694217

GI number: 209694217

Start: 690051

End: 691430

Strand: Direct

Name: radA [H]

Synonym: VSAL_I0626

Alternate gene names: 209694217

Gene position: 690051-691430 (Clockwise)

Preceding gene: 209694215

Following gene: 209694218

Centisome position: 20.75

GC content: 43.12

Gene sequence:

>1380_bases
ATGGCAAAAGCAAAACGTGCCTACGTTTGTAACGATTGTGGGGCAGACTTTCCTCGTTGGCAAGGACAGTGTAGTGCTTG
TGGGGCGTGGAACACGATTACAGAGGTTCGATTAGCGGCTTCTCCTCAAGTGGCTAGAAACGAACGATTGGTCGGATACT
CAGGTTCTGCTACGGATTCTAAAGTTCAAACGCTATCAAGTATTGATCTTCAAGAGGTCCCTCGTTTTAGTAGCAGCTTT
AAAGAGTTTGATCGGGTGCTTGGTGGTGGGATTGTTCCTGGTGCGGCTATCTTAATTGGCGGTAATCCGGGGGCTGGTAA
ATCCACACTGCTGTTGCAAACCATGTGTCGCTTAGCCAGTGAAATGCCAACGTTGTATGTAACGGGTGAAGAATCATTAC
AACAAGTCGCCATGCGAGCATCTCGTCTTGGGCTGCCAAAAGATAATTTGAAAATGTTGTCTGAAACCAGCATTGATAAA
ATTTGTCAGATCGCAGACAAAGAAAAACCTAAAATAATGGTTATTGACTCGATTCAAGTGATGCATGTAGATGATGTTCA
ATCGTCTCCAGGCAGTGTTGCTCAAGTTAGAGAATCAGCAACAACACTTACTCGTTATGCAAAACAAAATAATGTGTCTA
TATTTCTTGTGGGGCATGTAACTAAAGATGGTTCGCTTGCAGGACCAAAAGTACTGGAGCATATTATTGATTGTTCTGTA
TTACTTGATGGAAGTGCAGACAGCCGATTTAGAACATTGCGTTGTAATAAAAACCGTTTTGGTGCCGTAAATGAATTGGG
CGTATTTGCAATGACGGGGCAAGGATTGAAAGAAGTAAATAATCCGTCTGCGATTTTTCTATCCCGTGGCGAAGAGGAAA
CGTCAGGCAGTTCTGTTATGGTTGTTTGGGAAGGGACACGCCCGTTATTGGTTGAAATTCAAGCATTAGTCGATTATTCC
CAACTATCTAATCCACGTCGAGTTTCTGTTGGGCTAGATCAAAACCGTTTGTCGATGTTACTTGCTGTACTTCATAAACA
TGGTGGTCTGCAAATGGCGGATCAGGATGTCTTTGTCAATGTGGTTGGTGGCGTAAAAGTTGCAGAAACCAGTGCCGATT
TAGCATTGATAATGGCGCTATTATCTAGCTTTAAAGATCGTCCATTACCAAAAGATGTGGTTGTTTTTGGTGAAGTAGGG
CTTGCCGGTGAAATCCGTCCAGTTCCTAGTGGTCAAGAGCGTCTTATGGAAGCGTATAAACATGGATTTAAACGCGCGAT
TGTTCCTGCTGCTAATATGCCGAAAGGGGGGATTGAAGGCATGCAAATTCATGGCGTGAAAAAATTATCAGAGGCAATTG
ATGCTTTTGATGAACTATAA

Upstream 100 bases:

>100_bases
TAAGAATCTAATGGGTTTATCGGCACAATTGATAAAATATTTAATGGCATACGTTAAATATTCATTCCCCATACCTTCAA
ATACAGTGATTCGGTGATAT

Downstream 100 bases:

>100_bases
TCAATAATTATTATGAATTTGCACTATTCTTTGCATTTGAAATTGTGTAAATTCGTCTATAACTTGCTAAAGCAGAAAGT
AATCGGTGTCGATTGGGGCT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAKAKRAYVCNDCGADFPRWQGQCSACGAWNTITEVRLAASPQVARNERLVGYSGSATDSKVQTLSSIDLQEVPRFSSSF
KEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLASEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETSIDK
ICQIADKEKPKIMVIDSIQVMHVDDVQSSPGSVAQVRESATTLTRYAKQNNVSIFLVGHVTKDGSLAGPKVLEHIIDCSV
LLDGSADSRFRTLRCNKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYS
QLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVNVVGGVKVAETSADLALIMALLSSFKDRPLPKDVVVFGEVG
LAGEIRPVPSGQERLMEAYKHGFKRAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL

Sequences:

>Translated_459_residues
MAKAKRAYVCNDCGADFPRWQGQCSACGAWNTITEVRLAASPQVARNERLVGYSGSATDSKVQTLSSIDLQEVPRFSSSF
KEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLASEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETSIDK
ICQIADKEKPKIMVIDSIQVMHVDDVQSSPGSVAQVRESATTLTRYAKQNNVSIFLVGHVTKDGSLAGPKVLEHIIDCSV
LLDGSADSRFRTLRCNKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYS
QLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVNVVGGVKVAETSADLALIMALLSSFKDRPLPKDVVVFGEVG
LAGEIRPVPSGQERLMEAYKHGFKRAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL
>Mature_458_residues
AKAKRAYVCNDCGADFPRWQGQCSACGAWNTITEVRLAASPQVARNERLVGYSGSATDSKVQTLSSIDLQEVPRFSSSFK
EFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLASEMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETSIDKI
CQIADKEKPKIMVIDSIQVMHVDDVQSSPGSVAQVRESATTLTRYAKQNNVSIFLVGHVTKDGSLAGPKVLEHIIDCSVL
LDGSADSRFRTLRCNKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVMVVWEGTRPLLVEIQALVDYSQ
LSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVNVVGGVKVAETSADLALIMALLSSFKDRPLPKDVVVFGEVGL
AGEIRPVPSGQERLMEAYKHGFKRAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=80.8695652173913, Blast_Score=776, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49416; Mature: 49285

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKRAYVCNDCGADFPRWQGQCSACGAWNTITEVRLAASPQVARNERLVGYSGSATDS
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCHHCCCEEEECCCCCCCH
KVQTLSSIDLQEVPRFSSSFKEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLAS
HHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETSIDKICQIADKEKPKIMVIDSIQV
HCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEE
MHVDDVQSSPGSVAQVRESATTLTRYAKQNNVSIFLVGHVTKDGSLAGPKVLEHIIDCSV
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEE
LLDGSADSRFRTLRCNKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVM
EEECCCCCCEEEEEECCCCCCCHHHCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEEE
VVWEGTRPLLVEIQALVDYSQLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVN
EEECCCCCEEHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCEEEE
VVGGVKVAETSADLALIMALLSSFKDRPLPKDVVVFGEVGLAGEIRPVPSGQERLMEAYK
EECCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
HGFKRAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL
HCHHEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAKRAYVCNDCGADFPRWQGQCSACGAWNTITEVRLAASPQVARNERLVGYSGSATDS
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCHHCCCEEEECCCCCCCH
KVQTLSSIDLQEVPRFSSSFKEFDRVLGGGIVPGAAILIGGNPGAGKSTLLLQTMCRLAS
HHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
EMPTLYVTGEESLQQVAMRASRLGLPKDNLKMLSETSIDKICQIADKEKPKIMVIDSIQV
HCCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEE
MHVDDVQSSPGSVAQVRESATTLTRYAKQNNVSIFLVGHVTKDGSLAGPKVLEHIIDCSV
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCEE
LLDGSADSRFRTLRCNKNRFGAVNELGVFAMTGQGLKEVNNPSAIFLSRGEEETSGSSVM
EEECCCCCCEEEEEECCCCCCCHHHCCEEEECCCCHHHCCCCCEEEEECCCCCCCCCEEE
VVWEGTRPLLVEIQALVDYSQLSNPRRVSVGLDQNRLSMLLAVLHKHGGLQMADQDVFVN
EEECCCCCEEHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCEEEE
VVGGVKVAETSADLALIMALLSSFKDRPLPKDVVVFGEVGLAGEIRPVPSGQERLMEAYK
EECCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
HGFKRAIVPAANMPKGGIEGMQIHGVKKLSEAIDAFDEL
HCHHEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]