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The map label for this gene is cobD [H]

Identifier: 187736169

GI number: 187736169

Start: 2039754

End: 2040746

Strand: Reverse

Name: cobD [H]

Synonym: Amuc_1682

Alternate gene names: 187736169

Gene position: 2040746-2039754 (Counterclockwise)

Preceding gene: 187736170

Following gene: 187736168

Centisome position: 76.6

GC content: 62.64

Gene sequence:

>993_bases
ATGTTCCCGTGCCCGTTCATCCTGCCTGCGGCCTTCCTGCTGGATATTCTGGCGGGAGAACCGCCCAACAGGTTTCATCC
CGTCTGTCTGATCGGCCGGTGCGCGCTCCGCATGGAAACACTGGCGAGGCAAAGGTGGGGAGGAACTTTTCATGCGGGAA
TGGCGGCCGCGCTGGCAACCTGCATTGCCGCATGGTTCGGCTGCGCGGCGCTCTTCCTTCCCTTTTCCCTGTTACCCTCC
CCCTGGGCACCCTGGATTCCGTCCGTTCTGGTCATTTACATCTGCATGGCCCCGCGCAGCCTGGCCGAGCATGCCCGGAA
GGTGGAAAACGCCCTGCGCAGCGGGAACGCCGGAGAGGCCCGGCACTCCGTCTCCATGATCGTGGGACGGGATACGGAAC
GGATGGACGTCTACGGCATCGCCAGAGCGGCCATTGAAAGCGTGGCGGAGAACTTGACGGATGGCGTTTTTTCCACCCTG
TTCTGGGCCTCAGCAGGCTGCCTGGCAGGAGGTGCCTCCGGGGCGGCTTCCGCCGCGCTGGCGCATCGTGTTTTCAACAT
CCTGGATGCCATGTGGGGGAAAAAAAATGACCGGTACAGACACTTCGGCACCTTTGCCGCACGCACGGATGACGCGCTCA
ACTTCATCCCCGCACGCCTTATTTTACCGTGTATTTCCTTTTCCGCCCTGATGGTGGAAGGAACCTCCGCCCGGAGGGCG
CTCACCATGGGATGGGCCTTCCGGAACGCCCACGCCAGCCCCAATTCCGCATGGAGCGAAGCGGCCTTCGCTGGAGCGCT
GGGCCTCCGTCTCGGGGGCCCGGTCTCTTACAAGGGAATTCCTGCAAACTACCCCTGGATAGGAACAGGGCGAACCAAAG
CCTCCGCCAGCGACCTGGCCCTGGCCATTCGGCTCATGTGGACGACTGCCGCCACGGGTACGCTGGCGTTCACTCTCCTT
CTTCTCTTCACCCCCCACTTCTGGACATCATGA

Upstream 100 bases:

>100_bases
CTATGACCTGGACGGAGCGCTGGACCGCCTGGCGGACGTGGTCCGGAAACATCTGGACCTGAAAACCATTTACCGAGCTC
TCCAACTAAAACGCTGACCC

Downstream 100 bases:

>100_bases
ACAAACTGCATATCCCCCCGTTGGACGAACAGGCCGCAAAAGCGGCCCTGGAACATCAGAAAATTCTGGCCAAGCCTCCC
CTTGCCCTGGGAAAACTGGA

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MFPCPFILPAAFLLDILAGEPPNRFHPVCLIGRCALRMETLARQRWGGTFHAGMAAALATCIAAWFGCAALFLPFSLLPS
PWAPWIPSVLVIYICMAPRSLAEHARKVENALRSGNAGEARHSVSMIVGRDTERMDVYGIARAAIESVAENLTDGVFSTL
FWASAGCLAGGASGAASAALAHRVFNILDAMWGKKNDRYRHFGTFAARTDDALNFIPARLILPCISFSALMVEGTSARRA
LTMGWAFRNAHASPNSAWSEAAFAGALGLRLGGPVSYKGIPANYPWIGTGRTKASASDLALAIRLMWTTAATGTLAFTLL
LLFTPHFWTS

Sequences:

>Translated_330_residues
MFPCPFILPAAFLLDILAGEPPNRFHPVCLIGRCALRMETLARQRWGGTFHAGMAAALATCIAAWFGCAALFLPFSLLPS
PWAPWIPSVLVIYICMAPRSLAEHARKVENALRSGNAGEARHSVSMIVGRDTERMDVYGIARAAIESVAENLTDGVFSTL
FWASAGCLAGGASGAASAALAHRVFNILDAMWGKKNDRYRHFGTFAARTDDALNFIPARLILPCISFSALMVEGTSARRA
LTMGWAFRNAHASPNSAWSEAAFAGALGLRLGGPVSYKGIPANYPWIGTGRTKASASDLALAIRLMWTTAATGTLAFTLL
LLFTPHFWTS
>Mature_330_residues
MFPCPFILPAAFLLDILAGEPPNRFHPVCLIGRCALRMETLARQRWGGTFHAGMAAALATCIAAWFGCAALFLPFSLLPS
PWAPWIPSVLVIYICMAPRSLAEHARKVENALRSGNAGEARHSVSMIVGRDTERMDVYGIARAAIESVAENLTDGVFSTL
FWASAGCLAGGASGAASAALAHRVFNILDAMWGKKNDRYRHFGTFAARTDDALNFIPARLILPCISFSALMVEGTSARRA
LTMGWAFRNAHASPNSAWSEAAFAGALGLRLGGPVSYKGIPANYPWIGTGRTKASASDLALAIRLMWTTAATGTLAFTLL
LLFTPHFWTS

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35421; Mature: 35421

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPCPFILPAAFLLDILAGEPPNRFHPVCLIGRCALRMETLARQRWGGTFHAGMAAALAT
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CIAAWFGCAALFLPFSLLPSPWAPWIPSVLVIYICMAPRSLAEHARKVENALRSGNAGEA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCH
RHSVSMIVGRDTERMDVYGIARAAIESVAENLTDGVFSTLFWASAGCLAGGASGAASAAL
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHH
AHRVFNILDAMWGKKNDRYRHFGTFAARTDDALNFIPARLILPCISFSALMVEGTSARRA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
LTMGWAFRNAHASPNSAWSEAAFAGALGLRLGGPVSYKGIPANYPWIGTGRTKASASDLA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LAIRLMWTTAATGTLAFTLLLLFTPHFWTS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFPCPFILPAAFLLDILAGEPPNRFHPVCLIGRCALRMETLARQRWGGTFHAGMAAALAT
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CIAAWFGCAALFLPFSLLPSPWAPWIPSVLVIYICMAPRSLAEHARKVENALRSGNAGEA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCH
RHSVSMIVGRDTERMDVYGIARAAIESVAENLTDGVFSTLFWASAGCLAGGASGAASAAL
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHH
AHRVFNILDAMWGKKNDRYRHFGTFAARTDDALNFIPARLILPCISFSALMVEGTSARRA
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
LTMGWAFRNAHASPNSAWSEAAFAGALGLRLGGPVSYKGIPANYPWIGTGRTKASASDLA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LAIRLMWTTAATGTLAFTLLLLFTPHFWTS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA