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The map label for this gene is ispB [H]

Identifier: 187736086

GI number: 187736086

Start: 1920740

End: 1921750

Strand: Direct

Name: ispB [H]

Synonym: Amuc_1597

Alternate gene names: 187736086

Gene position: 1920740-1921750 (Clockwise)

Preceding gene: 187736069

Following gene: 187736089

Centisome position: 72.1

GC content: 54.9

Gene sequence:

>1011_bases
ATGTCCATTCCGTTCTTTTCGAAATTACGATCCCAGAAGCATTATCTTCAACTGGTTAAACCGGAATTGAAGCAGGTTTC
CGAGTTTGTTGAAGCGCAATCATCATGTTTTGACCCGGAAGTGGCCGATTACATGGAAACGGTATGCCAGTCCAAGGGTA
AAATGCTCCGTCCGGCCCTGGTTCTGCTGGTTGCCGGCGCTACGGGCGGCATCAAGCCTGCCCATATCCGCCTGGGTGCT
TTGCTGGAAATGGTGCATCTGGCTTCCCTTGTGCATGATGACGTGATTGACGAGGCCGACAAACGCCGGGACGAGGCTAC
GGCGAATGCCCTCTGGGGCAACAGCCTCGCCGTACTGCTGGGGGATGTCCTCTTTTCCCACGCCATGGTGCTGGGTACGG
AATTCGGCAGCACGGAATTCTGCCGCAGGCTGGCGAATACCGTCAGGAACGTATGCCAGGGGGAGGTAGCCCAGTCCAGC
CGCCTGTATGATCTGAGCATGACCCGCGAGGAATATTTTGAAATCATCCGGAAAAAAACCGCTTCCCTTTTCTCCGCAGC
TACGGGGGGGGCTGGCTGGATTTCCGGAGTAACCCCGGAAGTGGAGGAATCCCTTTACCAGCTGGGGGATCTGCTGGGTG
TGGCTTACCAGATTTACGACGACTGCCTGGACATGGTGGGAGATGAAGACGATGCCGGAAAGACGCTTCATACGGATGCC
ACCAAGGGCAAGCTGACCCTTCCCATCTTCAATTTGCTGGAATGCGGTGACAGGAATGTGGAAGCTACCCTGCGGGACGC
CATTGAAAACCGTGAAGTGGTGGATTACTCCGCCTTTCAGGACAATCCCGTTTTTGCAGAGGCTCTGGACAAGGCGATTC
AGGTAGCTCTGGAGAAAAACGAGGCGGCCCGCGAAATTCTGTGGCTCCTTCCCCAAACGGAATATCGTGAAGCTTTGGCG
GAAATGACCTTTTACATGGACGAGCTGCTTAACGACTGCCGTATCTCCTGA

Upstream 100 bases:

>100_bases
TCCGGTTATGAGAAAGATGCCCTGTGAAATACATTGCTAGGGAACTTTAAATATGCCACAATCCCTGCGGCGCTGACCAG
CGCCGCCCCCGTTCTATATC

Downstream 100 bases:

>100_bases
CCTGCTTCAAGGAGTTTTTGTCCGGTCATGCCCTCCTTATGTAATTTGTGTCTTCCGGCATTTTCCCAAAAGGAAACCTG
CCGGAGGGAATGGGAGGAGC

Product: Polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSIPFFSKLRSQKHYLQLVKPELKQVSEFVEAQSSCFDPEVADYMETVCQSKGKMLRPALVLLVAGATGGIKPAHIRLGA
LLEMVHLASLVHDDVIDEADKRRDEATANALWGNSLAVLLGDVLFSHAMVLGTEFGSTEFCRRLANTVRNVCQGEVAQSS
RLYDLSMTREEYFEIIRKKTASLFSAATGGAGWISGVTPEVEESLYQLGDLLGVAYQIYDDCLDMVGDEDDAGKTLHTDA
TKGKLTLPIFNLLECGDRNVEATLRDAIENREVVDYSAFQDNPVFAEALDKAIQVALEKNEAAREILWLLPQTEYREALA
EMTFYMDELLNDCRIS

Sequences:

>Translated_336_residues
MSIPFFSKLRSQKHYLQLVKPELKQVSEFVEAQSSCFDPEVADYMETVCQSKGKMLRPALVLLVAGATGGIKPAHIRLGA
LLEMVHLASLVHDDVIDEADKRRDEATANALWGNSLAVLLGDVLFSHAMVLGTEFGSTEFCRRLANTVRNVCQGEVAQSS
RLYDLSMTREEYFEIIRKKTASLFSAATGGAGWISGVTPEVEESLYQLGDLLGVAYQIYDDCLDMVGDEDDAGKTLHTDA
TKGKLTLPIFNLLECGDRNVEATLRDAIENREVVDYSAFQDNPVFAEALDKAIQVALEKNEAAREILWLLPQTEYREALA
EMTFYMDELLNDCRIS
>Mature_335_residues
SIPFFSKLRSQKHYLQLVKPELKQVSEFVEAQSSCFDPEVADYMETVCQSKGKMLRPALVLLVAGATGGIKPAHIRLGAL
LEMVHLASLVHDDVIDEADKRRDEATANALWGNSLAVLLGDVLFSHAMVLGTEFGSTEFCRRLANTVRNVCQGEVAQSSR
LYDLSMTREEYFEIIRKKTASLFSAATGGAGWISGVTPEVEESLYQLGDLLGVAYQIYDDCLDMVGDEDDAGKTLHTDAT
KGKLTLPIFNLLECGDRNVEATLRDAIENREVVDYSAFQDNPVFAEALDKAIQVALEKNEAAREILWLLPQTEYREALAE
MTFYMDELLNDCRIS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=293, Percent_Identity=30.0341296928328, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1789578, Length=280, Percent_Identity=36.7857142857143, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17505681, Length=232, Percent_Identity=33.1896551724138, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319475, Length=225, Percent_Identity=30.6666666666667, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325188, Length=219, Percent_Identity=25.5707762557078, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24651612, Length=231, Percent_Identity=30.3030303030303, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37334; Mature: 37203

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPFFSKLRSQKHYLQLVKPELKQVSEFVEAQSSCFDPEVADYMETVCQSKGKMLRPAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHH
VLLVAGATGGIKPAHIRLGALLEMVHLASLVHDDVIDEADKRRDEATANALWGNSLAVLL
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GDVLFSHAMVLGTEFGSTEFCRRLANTVRNVCQGEVAQSSRLYDLSMTREEYFEIIRKKT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHH
ASLFSAATGGAGWISGVTPEVEESLYQLGDLLGVAYQIYDDCLDMVGDEDDAGKTLHTDA
HHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
TKGKLTLPIFNLLECGDRNVEATLRDAIENREVVDYSAFQDNPVFAEALDKAIQVALEKN
CCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
EAAREILWLLPQTEYREALAEMTFYMDELLNDCRIS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPFFSKLRSQKHYLQLVKPELKQVSEFVEAQSSCFDPEVADYMETVCQSKGKMLRPAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHH
VLLVAGATGGIKPAHIRLGALLEMVHLASLVHDDVIDEADKRRDEATANALWGNSLAVLL
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GDVLFSHAMVLGTEFGSTEFCRRLANTVRNVCQGEVAQSSRLYDLSMTREEYFEIIRKKT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHH
ASLFSAATGGAGWISGVTPEVEESLYQLGDLLGVAYQIYDDCLDMVGDEDDAGKTLHTDA
HHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
TKGKLTLPIFNLLECGDRNVEATLRDAIENREVVDYSAFQDNPVFAEALDKAIQVALEKN
CCCEEEHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
EAAREILWLLPQTEYREALAEMTFYMDELLNDCRIS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]