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The map label for this gene is metG [H]

Identifier: 186476942

GI number: 186476942

Start: 2459745

End: 2461925

Strand: Direct

Name: metG [H]

Synonym: Bphy_2191

Alternate gene names: 186476942

Gene position: 2459745-2461925 (Clockwise)

Preceding gene: 186476941

Following gene: 186476943

Centisome position: 70.7

GC content: 63.14

Gene sequence:

>2181_bases
ATGTCAGCAACCGACGCCACCGCGGTTCAATCCGCGGACCTCACCGCCACCGGCGCGCCGAAGGCTCGCCGCCAGATCCT
CGTCACGTCTGCCCTGCCGTACGCGAACGGGCAAATTCATATCGGCCACCTGGTCGAATATATCCAGACGGACATCTGGG
TCCGCGCGCTGCGAATGCATGGGCACGAGGTCTACTACGTCGGCGCGGACGACACGCACGGCACGCCCATCATGCTGAAG
GCAGAGCAGGAAGGCATCACGCCCAAGCAGCTGATCGACCGCGTGTGGCAGGAGCACAAACGCGATTTCGACAACTTTGG
CATTTCGTTCGACAACTACTACTCGACCGATTCCGAAGAGAACCGCGTGCTGTCCGAGTCGATCTACACGGCGCTGCAAG
CGGCCGGTCTGATCGAGGCGCGCGACATCGAGCAGGCGTATGACCCCGTCAAGGAAATGTTCCTGCCGGACCGCTTCATC
AAGGGCGAGTGTCCCAAGTGCCACGCGAAGGATCAATACGGCGACAACTGCGAAGTATGCGGCTCGACCTACCAGCCCAC
GGAGTTGATCAACCCGTACTCCGTCGTGTCGGGCGCGACGCCTGTCAAGAAGACGTCGACGCACTACTTCTTCAGGCTGT
CCGACCCGCGCTGCGAGAATTTTCTGCGCGGCTGGGTAGGCGGTCTCGCGCAGCAGGAAGCGACCAACAAGATGCGCGAG
TGGCTCGGCGACGCGGGCGAAGCAAAGCTCGCGGACTGGGACATCTCGCGCGACGCGCCCTATTTCGGCTTCGAGATTCC
GGGCGCGCCCGGCAAATACTTTTACGTGTGGCTGGACGCGCCCGTCGGCTATTACGCGAGCTTACGCAACCTGGCCGAAA
AGCGCGGCCTCGATTTCGACGCGTGGACGAAAAAAGGCGCGACGACCGAGCAATATCATTTCATCGGCAAGGACATCCTG
TACTTCCACACGCTGTTCTGGCCGGCGATGCTCGAGTTCTCGGGTCACCGCACGCCAACCAACGTGTTCGCGCACGGCTT
CCTGACCGTCGATGGCGCGAAGATGTCGAAGTCGCGCGGCACGTTCATCACGGCCAATAGCGTGATCGAAACGGGCATGA
ATCCCGAGTGGCTGCGCTACTACTTCGCGGCCAAACTGAACAGCACGATGGAAGACATCGATCTGAATCTCGAGGACTTC
CAGGCGCGCGTGAACAGCGATCTCGTCGGCAAGTATGTGAACATCGCCAGCCGGGCGGCGGGCTTTCTGCTCAAGCGCTT
CGACGGCCGTGTGCAGGACAGCGCGATGCAGCACCCGCTGCTCGCGTCGCTGCGCGGTGCCATTGCGCAAATCGCCGCGC
ACTACGAGGCACGCGACTACGGCCGCGCGCTGCGCCTGACGATGGAACTCGCGGACTCCGTCAATGCGTATGTCGACACG
GCCAAGCCGTGGGATCAGGCGAAGGATCCCGCCAACGCGGTCGCGCTGCACGAAACGTGCAGCGTGTCGCTGGAGGCGTT
CCGCCTGCTGTCGCTCGCGCTCAAGCCCGTGTTGCCGAAGGTCGCGGAAGCGGCCGAGGCGTTCCTCGGCATCGAGCCGC
TCAAGTGGACGGACGCCGACGTGCCGCTGCGTTCGACGCACGCGATCAACGCGTACAAACATCTGATGACGCGCGTCGAT
CCGAAGCAGATCGAGGCGCTGCTCGCGGCGAACCGCGGCTCGCTGCAACCGGCTGCATCGGCTGAAGCGCAGTCCGCGGC
TGCCAAGGCCAAAGCCAAGGCGCACGCGAAAGCGACGGCGGCCGCCCACGGCGACGCCTCGGCAAAGCCCGACGACAGCG
GCATCATCTCGATCGACGACTTCGCGAAGATCGATCTGCGCATTGCGAAGATCGTCGATTGCAAGGCGGTCGAAGGCTCG
GACAAGCTGCTGCAACTGACGCTCGACGTCGGCGAAGAAAAGACGCGCAACGTGTTTTCCGGAATCAAGTCGGCGTATCA
GCCGGAGCAACTGGTCGGCAAGTTGACTGTGATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGCTTGTCGGAAG
GCATGGTGCTCGCGGCATCGGCAGCAGACGAGAAGGCCGAACCGGGCATCTACGTGCTCGAGCCGGACAGCGGCGCGAAG
CCGGGCATGCGCGTCAAATAA

Upstream 100 bases:

>100_bases
AGCGGACATTCCCGCCCGTGCGGTCCTGGGCACAGCGCCGTCCGCCGACGTCGAACGTCGTGACGGCCGCCCGTACCTTG
CGCCAGTCCATAGAACCCAC

Downstream 100 bases:

>100_bases
CGCACTCATCGATCTACCGGCGCACAGCCGTCAACCAGGAAAGCACGTCTTCGGACGTGCTTTTTTTTCGTCCGGAACCG
AGTTCTGGCGACCGGCTCCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSATDATAVQSADLTATGAPKARRQILVTSALPYANGQIHIGHLVEYIQTDIWVRALRMHGHEVYYVGADDTHGTPIMLK
AEQEGITPKQLIDRVWQEHKRDFDNFGISFDNYYSTDSEENRVLSESIYTALQAAGLIEARDIEQAYDPVKEMFLPDRFI
KGECPKCHAKDQYGDNCEVCGSTYQPTELINPYSVVSGATPVKKTSTHYFFRLSDPRCENFLRGWVGGLAQQEATNKMRE
WLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASLRNLAEKRGLDFDAWTKKGATTEQYHFIGKDIL
YFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITANSVIETGMNPEWLRYYFAAKLNSTMEDIDLNLEDF
QARVNSDLVGKYVNIASRAAGFLLKRFDGRVQDSAMQHPLLASLRGAIAQIAAHYEARDYGRALRLTMELADSVNAYVDT
AKPWDQAKDPANAVALHETCSVSLEAFRLLSLALKPVLPKVAEAAEAFLGIEPLKWTDADVPLRSTHAINAYKHLMTRVD
PKQIEALLAANRGSLQPAASAEAQSAAAKAKAKAHAKATAAAHGDASAKPDDSGIISIDDFAKIDLRIAKIVDCKAVEGS
DKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGIYVLEPDSGAK
PGMRVK

Sequences:

>Translated_726_residues
MSATDATAVQSADLTATGAPKARRQILVTSALPYANGQIHIGHLVEYIQTDIWVRALRMHGHEVYYVGADDTHGTPIMLK
AEQEGITPKQLIDRVWQEHKRDFDNFGISFDNYYSTDSEENRVLSESIYTALQAAGLIEARDIEQAYDPVKEMFLPDRFI
KGECPKCHAKDQYGDNCEVCGSTYQPTELINPYSVVSGATPVKKTSTHYFFRLSDPRCENFLRGWVGGLAQQEATNKMRE
WLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASLRNLAEKRGLDFDAWTKKGATTEQYHFIGKDIL
YFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITANSVIETGMNPEWLRYYFAAKLNSTMEDIDLNLEDF
QARVNSDLVGKYVNIASRAAGFLLKRFDGRVQDSAMQHPLLASLRGAIAQIAAHYEARDYGRALRLTMELADSVNAYVDT
AKPWDQAKDPANAVALHETCSVSLEAFRLLSLALKPVLPKVAEAAEAFLGIEPLKWTDADVPLRSTHAINAYKHLMTRVD
PKQIEALLAANRGSLQPAASAEAQSAAAKAKAKAHAKATAAAHGDASAKPDDSGIISIDDFAKIDLRIAKIVDCKAVEGS
DKLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGIYVLEPDSGAK
PGMRVK
>Mature_725_residues
SATDATAVQSADLTATGAPKARRQILVTSALPYANGQIHIGHLVEYIQTDIWVRALRMHGHEVYYVGADDTHGTPIMLKA
EQEGITPKQLIDRVWQEHKRDFDNFGISFDNYYSTDSEENRVLSESIYTALQAAGLIEARDIEQAYDPVKEMFLPDRFIK
GECPKCHAKDQYGDNCEVCGSTYQPTELINPYSVVSGATPVKKTSTHYFFRLSDPRCENFLRGWVGGLAQQEATNKMREW
LGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASLRNLAEKRGLDFDAWTKKGATTEQYHFIGKDILY
FHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITANSVIETGMNPEWLRYYFAAKLNSTMEDIDLNLEDFQ
ARVNSDLVGKYVNIASRAAGFLLKRFDGRVQDSAMQHPLLASLRGAIAQIAAHYEARDYGRALRLTMELADSVNAYVDTA
KPWDQAKDPANAVALHETCSVSLEAFRLLSLALKPVLPKVAEAAEAFLGIEPLKWTDADVPLRSTHAINAYKHLMTRVDP
KQIEALLAANRGSLQPAASAEAQSAAAKAKAKAHAKATAAAHGDASAKPDDSGIISIDDFAKIDLRIAKIVDCKAVEGSD
KLLQLTLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGIYVLEPDSGAKP
GMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=27.5800711743772, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=26.5420560747664, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1788432, Length=707, Percent_Identity=54.8797736916549, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=35.4545454545455, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=617, Percent_Identity=26.904376012966, Blast_Score=229, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=546, Percent_Identity=23.4432234432234, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71992781, Length=281, Percent_Identity=25.2669039145907, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=28.3687943262411, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=24.0437158469945, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24655311, Length=628, Percent_Identity=28.6624203821656, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI21358483, Length=524, Percent_Identity=26.5267175572519, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79970; Mature: 79839

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATDATAVQSADLTATGAPKARRQILVTSALPYANGQIHIGHLVEYIQTDIWVRALRMH
CCCCCCCHHCCCCCCCCCCCHHHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHC
GHEVYYVGADDTHGTPIMLKAEQEGITPKQLIDRVWQEHKRDFDNFGISFDNYYSTDSEE
CCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHH
NRVLSESIYTALQAAGLIEARDIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDNCEVC
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCC
GSTYQPTELINPYSVVSGATPVKKTSTHYFFRLSDPRCENFLRGWVGGLAQQEATNKMRE
CCCCCCHHHCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
WLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASLRNLAEKRGLDFD
HHCCCCCCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCH
AWTKKGATTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCHHCCCCC
TFITANSVIETGMNPEWLRYYFAAKLNSTMEDIDLNLEDFQARVNSDLVGKYVNIASRAA
CEEEECHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHH
GFLLKRFDGRVQDSAMQHPLLASLRGAIAQIAAHYEARDYGRALRLTMELADSVNAYVDT
HHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AKPWDQAKDPANAVALHETCSVSLEAFRLLSLALKPVLPKVAEAAEAFLGIEPLKWTDAD
CCCCHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VPLRSTHAINAYKHLMTRVDPKQIEALLAANRGSLQPAASAEAQSAAAKAKAKAHAKATA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAHGDASAKPDDSGIISIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTRNVFS
HCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEECEECCCCCCEEEEEECCCCHHHHHHHH
GIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGIYVLEPDSGAK
HHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCC
PGMRVK
CCCCCC
>Mature Secondary Structure 
SATDATAVQSADLTATGAPKARRQILVTSALPYANGQIHIGHLVEYIQTDIWVRALRMH
CCCCCCHHCCCCCCCCCCCHHHHEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHC
GHEVYYVGADDTHGTPIMLKAEQEGITPKQLIDRVWQEHKRDFDNFGISFDNYYSTDSEE
CCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCHH
NRVLSESIYTALQAAGLIEARDIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDNCEVC
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCC
GSTYQPTELINPYSVVSGATPVKKTSTHYFFRLSDPRCENFLRGWVGGLAQQEATNKMRE
CCCCCCHHHCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
WLGDAGEAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASLRNLAEKRGLDFD
HHCCCCCCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCH
AWTKKGATTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCHHCCCCC
TFITANSVIETGMNPEWLRYYFAAKLNSTMEDIDLNLEDFQARVNSDLVGKYVNIASRAA
CEEEECHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHH
GFLLKRFDGRVQDSAMQHPLLASLRGAIAQIAAHYEARDYGRALRLTMELADSVNAYVDT
HHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AKPWDQAKDPANAVALHETCSVSLEAFRLLSLALKPVLPKVAEAAEAFLGIEPLKWTDAD
CCCCHHCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VPLRSTHAINAYKHLMTRVDPKQIEALLAANRGSLQPAASAEAQSAAAKAKAKAHAKATA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAHGDASAKPDDSGIISIDDFAKIDLRIAKIVDCKAVEGSDKLLQLTLDVGEEKTRNVFS
HCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEECEECCCCCCEEEEEECCCCHHHHHHHH
GIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGIYVLEPDSGAK
HHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCC
PGMRVK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA