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The map label for this gene is lptD [H]

Identifier: 170746988

GI number: 170746988

Start: 594472

End: 597051

Strand: Reverse

Name: lptD [H]

Synonym: Mrad2831_0554

Alternate gene names: 170746988

Gene position: 597051-594472 (Counterclockwise)

Preceding gene: 170746989

Following gene: 170746987

Centisome position: 9.82

GC content: 68.26

Gene sequence:

>2580_bases
TTGCGTAAGGTCGCCGACATCCTGCGGAAGGCTGGTTTCCGGGCCGCGCTGGCGCTCGCCGCGCCGCTGGCGGGGGGCCT
GTCCGCCCATGCCCAGGGCCTGCCCGCCGTCGCGGCCGGCAAGCCCGGCGACAAGCTGCTCGTCGAGGCCGACGAGCTGA
TCTACGACAACGACCGGGGCACAGTGACGGCGCGGGGCAACGCCGAGCTGCATTACGGCCCGCGGACCCTGCAGGCCGAC
CGCGTGCGCTACGACCGGAATTCCGGCCGGGTCTTCGCTGAGGGCAATGTCCGCCTCACCGAGGCCGACGGCGGCGTCGT
GACCGGCGCGCGCATGGAACTCACCGACGACTTCAAGACCGGCTTCGTCGACGCTTTCCGCGGCCAGCAGACCGTCCAGC
GCAAGTTCGAGACGGTGCGCACCCGGTTCTCCGCGCCGCGGGCCGAGCGCCTGAACGGCGAGCAGACCAGCTTCGAGTCC
GGCAGCTACACGGCCTGCGAGCCGTGCAAGGACAACCCTGAGACGCCGCCGCTGTGGCAGATCAAGGCGAAGAAGATCAT
CCACGACAACGAGACCCACACGGTCTACTTCGACGATTCGACGCTCGAGATCGCCGGCATCCCGGTGGCCTACCTGCCGT
ATTTCGAGGCGCCGGACCCGACGGTGAAGCGCAAGACCGGCTTCCTGACGCCGCGCTTCGTCACGACGACCGCGCTCGGC
AACGGCGTGTCGCTCCCGTACTTCATCAACCTCGCGCCGAGCTACGACCTGACGCTGACGCCGACCCTGCTCAGCCGCCA
GGGCCTGCTCGCGCAGGGCGAGTTCCGCCAGCGGATCGACAACGGCTTCTACAACATCCGCCTGTCGGGCATCTCGCAGA
CGACGCCCTCGGCCTTCCTCCCGAGCCCGCTCGGCGCCGGCGAGCGGGACTTCCGCGGCTCGGTCGAATCGCAGGGCCGG
TTCTACATCAACCCGCGCTGGCGCACGGGCTGGGACCTTGTCGGCGTGACCGACAAGTGGTTCCTCGACAATTACCGGAT
CCGCAACCAGAACATCACCACGGACTATTTCCGCGAGGCGACCTCGACCGCCTTCCTGATCGGCCAGGGCGACCGCTCGT
GGTTCGAGGCCCGGGGCTACTACTTCAAGGGCCTGTCGAGCTTCGATTGGCAGAAGCAGCAGCCGATCGTCGCGCCGGTG
ATCGACTACGACAAGCGGGTCAACGGCCCCTCGGAGATCGGCGGCGAGGTGCGCTTCCAGGCGAACATCACCAGCCTGAC
CCGCGAGACGACCCAGTACCAGGGCCTGCCGCGGACCTCGAGCTACCTGTTCTCGCCGAGCGTCAACGGTGTCAGCTTCC
CGCTCTACCAGACCTGCACGGTCTTCCAGCGCGGCCTGTGCGCCATCGACGGGCTTGCCGGCACCAACACCCGCGCCTCC
GCGGAGCTGTCCTGGCGCCGCAGCTTCATCGACGACTGGGGCCAGAAGTTCACGCCGTTCGCGTACCTGCGCACCGACGC
GTTCTTCACCAACCCGAGCTTCTCGGGCTACCAGAACGACTTGGCCCCGCAGGTCGCCAAGATCGACGACGGCTTCGCCG
GGCGGGTCATGCCGGCGGTCGGCCTCGACTACCGCTACCCGTTCGTCGCCAATTTCGGCGCGCTCGGCGTCCACACCCTG
GAGCCGATCGGCCAGATCATCGCGCGCCCGTCCGAGACCCGGATCGGCCGCCTGCCGAACGAGGACGCGCAGAGCCTCGT
CTTCGACGACACCTCGCTGTTCGAGTGGGACAAGTTCTCGGGCTACGACCGGGTCGAGGGCGGCGTGCGCACCAACCTCG
GCGGCCAGTACTCCGTCGTGACGCCGTCGGGCTGGTACGCGAACGTCCTGTTCGGCGAGTCCATCCAGCTCGCGGGCGTC
AACTCGTTCCGCCGGGGCGACATCGCCAATGTCGGCCTGGATTCCGGGCTCGAGACGCAGCGGTCGGACTTCGTCGGCCG
CTTCCAGGTCTCGCCCAACCAGAACATCACGTTCATCAGCCGGGCCCGGTTCAACCAGTCCGACTTCCACGTGGCGCGGT
TCGAGACCGGCGCGACGGCGCGGTTCGCGCCGTTCCTGCCGCTCACCGTCTCGGCCTTCTACTCGTACTACGAGGCGCAG
CCGCTGCTGGGCTACAGCCACTACCGCGAGGGCATCACCGCCACCGCGACCTACAACATCACGCCGAACTGGTTCGTGTC
GGGCTCGCTGCTCGTCGACCTGACCCACTACCTCGACGTGCGCAACACCTACACGGACGCGCTGAGCTCGTACCTGGCAA
ACCCGATCGGGACGGTGCCGATCTACCAGAACCCGGGCCGCTTCTACCTGTCGGGCGCAAGCTTCGGCGGCGGCTACCAG
GATGAGTGCACCACCGTCTCGCTGAACTACATCAACTCGCCGATCGCCACCGCGACGGGCGTGCGCGAGCGCAACCAGAC
GGTGCTGCTGCGGGTCGAGCTGAAGACGCTGGGTGAGGCCGACCTGCGCCAGAACGTCGGCACCGCCACCACCGCGGACG
GCATCGCCTCGGTGCGGTGA

Upstream 100 bases:

>100_bases
TTCTGTACCAGGAAGACGGCTGACTTCCGCGATCCTTCGCGGGACGGGAGACGGGTGTTGAGGTTGGTGGCGGCAGACGT
CACAGGGGTTGGGACGCGCG

Downstream 100 bases:

>100_bases
GGAACCGGGACCGGCGGCGCTCCGTTTCGGCGCCGCGTCCGTCGCGGTCCCATCGCGCGCCGGGCGGCGGGATCCGGCTC
CGCGCACGGCCGCGCTCTGC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MRKVADILRKAGFRAALALAAPLAGGLSAHAQGLPAVAAGKPGDKLLVEADELIYDNDRGTVTARGNAELHYGPRTLQAD
RVRYDRNSGRVFAEGNVRLTEADGGVVTGARMELTDDFKTGFVDAFRGQQTVQRKFETVRTRFSAPRAERLNGEQTSFES
GSYTACEPCKDNPETPPLWQIKAKKIIHDNETHTVYFDDSTLEIAGIPVAYLPYFEAPDPTVKRKTGFLTPRFVTTTALG
NGVSLPYFINLAPSYDLTLTPTLLSRQGLLAQGEFRQRIDNGFYNIRLSGISQTTPSAFLPSPLGAGERDFRGSVESQGR
FYINPRWRTGWDLVGVTDKWFLDNYRIRNQNITTDYFREATSTAFLIGQGDRSWFEARGYYFKGLSSFDWQKQQPIVAPV
IDYDKRVNGPSEIGGEVRFQANITSLTRETTQYQGLPRTSSYLFSPSVNGVSFPLYQTCTVFQRGLCAIDGLAGTNTRAS
AELSWRRSFIDDWGQKFTPFAYLRTDAFFTNPSFSGYQNDLAPQVAKIDDGFAGRVMPAVGLDYRYPFVANFGALGVHTL
EPIGQIIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFSGYDRVEGGVRTNLGGQYSVVTPSGWYANVLFGESIQLAGV
NSFRRGDIANVGLDSGLETQRSDFVGRFQVSPNQNITFISRARFNQSDFHVARFETGATARFAPFLPLTVSAFYSYYEAQ
PLLGYSHYREGITATATYNITPNWFVSGSLLVDLTHYLDVRNTYTDALSSYLANPIGTVPIYQNPGRFYLSGASFGGGYQ
DECTTVSLNYINSPIATATGVRERNQTVLLRVELKTLGEADLRQNVGTATTADGIASVR

Sequences:

>Translated_859_residues
MRKVADILRKAGFRAALALAAPLAGGLSAHAQGLPAVAAGKPGDKLLVEADELIYDNDRGTVTARGNAELHYGPRTLQAD
RVRYDRNSGRVFAEGNVRLTEADGGVVTGARMELTDDFKTGFVDAFRGQQTVQRKFETVRTRFSAPRAERLNGEQTSFES
GSYTACEPCKDNPETPPLWQIKAKKIIHDNETHTVYFDDSTLEIAGIPVAYLPYFEAPDPTVKRKTGFLTPRFVTTTALG
NGVSLPYFINLAPSYDLTLTPTLLSRQGLLAQGEFRQRIDNGFYNIRLSGISQTTPSAFLPSPLGAGERDFRGSVESQGR
FYINPRWRTGWDLVGVTDKWFLDNYRIRNQNITTDYFREATSTAFLIGQGDRSWFEARGYYFKGLSSFDWQKQQPIVAPV
IDYDKRVNGPSEIGGEVRFQANITSLTRETTQYQGLPRTSSYLFSPSVNGVSFPLYQTCTVFQRGLCAIDGLAGTNTRAS
AELSWRRSFIDDWGQKFTPFAYLRTDAFFTNPSFSGYQNDLAPQVAKIDDGFAGRVMPAVGLDYRYPFVANFGALGVHTL
EPIGQIIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFSGYDRVEGGVRTNLGGQYSVVTPSGWYANVLFGESIQLAGV
NSFRRGDIANVGLDSGLETQRSDFVGRFQVSPNQNITFISRARFNQSDFHVARFETGATARFAPFLPLTVSAFYSYYEAQ
PLLGYSHYREGITATATYNITPNWFVSGSLLVDLTHYLDVRNTYTDALSSYLANPIGTVPIYQNPGRFYLSGASFGGGYQ
DECTTVSLNYINSPIATATGVRERNQTVLLRVELKTLGEADLRQNVGTATTADGIASVR
>Mature_859_residues
MRKVADILRKAGFRAALALAAPLAGGLSAHAQGLPAVAAGKPGDKLLVEADELIYDNDRGTVTARGNAELHYGPRTLQAD
RVRYDRNSGRVFAEGNVRLTEADGGVVTGARMELTDDFKTGFVDAFRGQQTVQRKFETVRTRFSAPRAERLNGEQTSFES
GSYTACEPCKDNPETPPLWQIKAKKIIHDNETHTVYFDDSTLEIAGIPVAYLPYFEAPDPTVKRKTGFLTPRFVTTTALG
NGVSLPYFINLAPSYDLTLTPTLLSRQGLLAQGEFRQRIDNGFYNIRLSGISQTTPSAFLPSPLGAGERDFRGSVESQGR
FYINPRWRTGWDLVGVTDKWFLDNYRIRNQNITTDYFREATSTAFLIGQGDRSWFEARGYYFKGLSSFDWQKQQPIVAPV
IDYDKRVNGPSEIGGEVRFQANITSLTRETTQYQGLPRTSSYLFSPSVNGVSFPLYQTCTVFQRGLCAIDGLAGTNTRAS
AELSWRRSFIDDWGQKFTPFAYLRTDAFFTNPSFSGYQNDLAPQVAKIDDGFAGRVMPAVGLDYRYPFVANFGALGVHTL
EPIGQIIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFSGYDRVEGGVRTNLGGQYSVVTPSGWYANVLFGESIQLAGV
NSFRRGDIANVGLDSGLETQRSDFVGRFQVSPNQNITFISRARFNQSDFHVARFETGATARFAPFLPLTVSAFYSYYEAQ
PLLGYSHYREGITATATYNITPNWFVSGSLLVDLTHYLDVRNTYTDALSSYLANPIGTVPIYQNPGRFYLSGASFGGGYQ
DECTTVSLNYINSPIATATGVRERNQTVLLRVELKTLGEADLRQNVGTATTADGIASVR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=129, Percent_Identity=32.5581395348837, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR007543 [H]

Pfam domain/function: PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 95120; Mature: 95120

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVADILRKAGFRAALALAAPLAGGLSAHAQGLPAVAAGKPGDKLLVEADELIYDNDRG
CCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCCEEECCCCCCEEEEEECCEEEECCCC
TVTARGNAELHYGPRTLQADRVRYDRNSGRVFAEGNVRLTEADGGVVTGARMELTDDFKT
EEEECCCCEEEECCCCCCHHHEEEECCCCEEEEECCEEEEECCCCEEECCEEEECCCHHH
GFVDAFRGQQTVQRKFETVRTRFSAPRAERLNGEQTSFESGSYTACEPCKDNPETPPLWQ
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCEEE
IKAKKIIHDNETHTVYFDDSTLEIAGIPVAYLPYFEAPDPTVKRKTGFLTPRFVTTTALG
EEEEEEEECCCCEEEEECCCEEEEECCCEEECCCCCCCCCCCHHHCCCCCCCEEEEEECC
NGVSLPYFINLAPSYDLTLTPTLLSRQGLLAQGEFRQRIDNGFYNIRLSGISQTTPSAFL
CCCCCCEEEEECCCCCEEECHHHHCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCC
PSPLGAGERDFRGSVESQGRFYINPRWRTGWDLVGVTDKWFLDNYRIRNQNITTDYFREA
CCCCCCCCHHHCCCCCCCCEEEECCCCCCCCEEEECCHHEECCCEEEECCCCCHHHHHHC
TSTAFLIGQGDRSWFEARGYYFKGLSSFDWQKQQPIVAPVIDYDKRVNGPSEIGGEVRFQ
CCEEEEEECCCCCHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHCCCEEEEE
ANITSLTRETTQYQGLPRTSSYLFSPSVNGVSFPLYQTCTVFQRGLCAIDGLAGTNTRAS
EEHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCHHHHHHHHHCCCEEECCCCCCCCCCC
AELSWRRSFIDDWGQKFTPFAYLRTDAFFTNPSFSGYQNDLAPQVAKIDDGFAGRVMPAV
CHHHHHHHHHHHHCCCCCCEEEEEECEEEECCCCCCCCHHCCCHHHHCCCCCCCCEEHHC
GLDYRYPFVANFGALGVHTLEPIGQIIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFS
CCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCC
GYDRVEGGVRTNLGGQYSVVTPSGWYANVLFGESIQLAGVNSFRRGDIANVGLDSGLETQ
CCCHHCCCEEECCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCH
RSDFVGRFQVSPNQNITFISRARFNQSDFHVARFETGATARFAPFLPLTVSAFYSYYEAQ
HHCCEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHCC
PLLGYSHYREGITATATYNITPNWFVSGSLLVDLTHYLDVRNTYTDALSSYLANPIGTVP
CCCCHHHHHCCEEEEEEEECCCCEEECCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCE
IYQNPGRFYLSGASFGGGYQDECTTVSLNYINSPIATATGVRERNQTVLLRVELKTLGEA
EEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHCCCCCCCCEEEEEEEECCCCCH
DLRQNVGTATTADGIASVR
HHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKVADILRKAGFRAALALAAPLAGGLSAHAQGLPAVAAGKPGDKLLVEADELIYDNDRG
CCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCCEEECCCCCCEEEEEECCEEEECCCC
TVTARGNAELHYGPRTLQADRVRYDRNSGRVFAEGNVRLTEADGGVVTGARMELTDDFKT
EEEECCCCEEEECCCCCCHHHEEEECCCCEEEEECCEEEEECCCCEEECCEEEECCCHHH
GFVDAFRGQQTVQRKFETVRTRFSAPRAERLNGEQTSFESGSYTACEPCKDNPETPPLWQ
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCEEE
IKAKKIIHDNETHTVYFDDSTLEIAGIPVAYLPYFEAPDPTVKRKTGFLTPRFVTTTALG
EEEEEEEECCCCEEEEECCCEEEEECCCEEECCCCCCCCCCCHHHCCCCCCCEEEEEECC
NGVSLPYFINLAPSYDLTLTPTLLSRQGLLAQGEFRQRIDNGFYNIRLSGISQTTPSAFL
CCCCCCEEEEECCCCCEEECHHHHCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCC
PSPLGAGERDFRGSVESQGRFYINPRWRTGWDLVGVTDKWFLDNYRIRNQNITTDYFREA
CCCCCCCCHHHCCCCCCCCEEEECCCCCCCCEEEECCHHEECCCEEEECCCCCHHHHHHC
TSTAFLIGQGDRSWFEARGYYFKGLSSFDWQKQQPIVAPVIDYDKRVNGPSEIGGEVRFQ
CCEEEEEECCCCCHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHCCCEEEEE
ANITSLTRETTQYQGLPRTSSYLFSPSVNGVSFPLYQTCTVFQRGLCAIDGLAGTNTRAS
EEHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCHHHHHHHHHCCCEEECCCCCCCCCCC
AELSWRRSFIDDWGQKFTPFAYLRTDAFFTNPSFSGYQNDLAPQVAKIDDGFAGRVMPAV
CHHHHHHHHHHHHCCCCCCEEEEEECEEEECCCCCCCCHHCCCHHHHCCCCCCCCEEHHC
GLDYRYPFVANFGALGVHTLEPIGQIIARPSETRIGRLPNEDAQSLVFDDTSLFEWDKFS
CCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCC
GYDRVEGGVRTNLGGQYSVVTPSGWYANVLFGESIQLAGVNSFRRGDIANVGLDSGLETQ
CCCHHCCCEEECCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCH
RSDFVGRFQVSPNQNITFISRARFNQSDFHVARFETGATARFAPFLPLTVSAFYSYYEAQ
HHCCEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHCC
PLLGYSHYREGITATATYNITPNWFVSGSLLVDLTHYLDVRNTYTDALSSYLANPIGTVP
CCCCHHHHHCCEEEEEEEECCCCEEECCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCE
IYQNPGRFYLSGASFGGGYQDECTTVSLNYINSPIATATGVRERNQTVLLRVELKTLGEA
EEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHCCCCCCCCEEEEEEEECCCCCH
DLRQNVGTATTADGIASVR
HHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA