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The map label for this gene is kbl [C]

Identifier: 170026352

GI number: 170026352

Start: 4577441

End: 4578652

Strand: Reverse

Name: kbl [C]

Synonym: YPK_4145

Alternate gene names: 170026352

Gene position: 4578652-4577441 (Counterclockwise)

Preceding gene: 170026359

Following gene: 170026351

Centisome position: 97.64

GC content: 50.08

Gene sequence:

>1212_bases
ATGTCCCTACCTTGTAACAAGCACCCCTTTTATCAGCAATTAGAACAACAACTCGACACGACTCGTACCGAAGGGTTGTA
TAAAAACGAACGCGTTATTACTTCCGCACAGCAAGCGAATATCGCTGTTGCTGATGGCAGCCGGGTGATTAACTTTTGCG
CCAATAACTATCTAGGGCTGGCAAATCATCCAAAACTTATTGCAGCCGCTAAAGCCGGCATGGATTCCCATGGGTTTGGC
ATGGCTTCAGTCCGCTTTATCTGTGGTACCCAAGACATACATAAAGAGCTCGAACATAAGCTCGCCAGTTTCCTCGGGAT
GGAAGACGCTATCCTGTACTCCTCTTGCTTTGATGCTAACGGCGGCCTATTTGAAACCCTGTTAGGGCCTGAAGATGCCA
TTATTTCCGATGCGTTGAATCATGCCTCAATCATTGATGGCGTACGGTTATGTAAAGCCAAGCGTTATCGTTATGCCAAC
AATGATATGACAGAGCTGGAAGCGCAGCTAAAACAAGCGAAAGCGGAGGGGGCTCGCCATATCATGATTGCCACCGATGG
CGTGTTCTCCATGGATGGTGTGATTGCTAACTTAAAAAGGGTGTGTGACCTTGCTGATGAATATCAGGCGCTGGTTATGG
TCGATGATTCACATGCGGTGGGGGTGATTGGTGCGAATGGGCGGGGGACTCATGAATACTGCGAAGTGATGGATCGCGTT
GATATCATCACTGGTACCTTGGGCAAAGCACTCGGCGGTGCATCTGGCGGTTATACTGCCGGGCGCAAAGAGTTGGTCGA
GTGGCTGCGCCAGCGTTCACGGCCTTATCTATTTTCAAACTCACTGGCTCCAGCGATTGTCGCCGCGTCCATTGAAGTCT
TGTCACTGCTGGAAGAGGGAGGCGCATTGCGTGATCGTCTATGGGCGAATGCGCGTTTATTCCGTGAGAAAATGAGTGCT
GCTGGTTTCACTTTGGCGGGTGCTGATCATGCCATTATTCCTGTCATGTTGGGTGAGGCTAAACTGGCGCAGGATTTTGC
CAATGCCTTGCTGAAAGAAGGCATTTATGTCACTGGCTTCTTCTTCCCTGTCGTGCCGAAGGGCCAGGCCCGTATTCGTA
CCCAGATGTCTGCAGGCCATACCACTGAGCAAGTTGAACGTGCCATCGAGGCATTTGTGCGTATTGGTAAGCAACTTAAC
GTTATTGCGTAA

Upstream 100 bases:

>100_bases
ATATGCTACATTTTCGACAATATAAGATAAGGTTTCTCGTCAGTTTGCCCACCAAGCAGTAAAATATTGTGTGCAATTGC
CAATCCTGGAGATAACCGAA

Downstream 100 bases:

>100_bases
GGGATTTTCATGAAAGCACTTTCTAAACTGAAAGCAGAAGAAGGCATTTGGATGACCGATGTGCCACAGCCAGAGCTGGG
TCACAACGATATCATGATCA

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSLPCNKHPFYQQLEQQLDTTRTEGLYKNERVITSAQQANIAVADGSRVINFCANNYLGLANHPKLIAAAKAGMDSHGFG
MASVRFICGTQDIHKELEHKLASFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYAN
NDMTELEAQLKQAKAEGARHIMIATDGVFSMDGVIANLKRVCDLADEYQALVMVDDSHAVGVIGANGRGTHEYCEVMDRV
DIITGTLGKALGGASGGYTAGRKELVEWLRQRSRPYLFSNSLAPAIVAASIEVLSLLEEGGALRDRLWANARLFREKMSA
AGFTLAGADHAIIPVMLGEAKLAQDFANALLKEGIYVTGFFFPVVPKGQARIRTQMSAGHTTEQVERAIEAFVRIGKQLN
VIA

Sequences:

>Translated_403_residues
MSLPCNKHPFYQQLEQQLDTTRTEGLYKNERVITSAQQANIAVADGSRVINFCANNYLGLANHPKLIAAAKAGMDSHGFG
MASVRFICGTQDIHKELEHKLASFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYAN
NDMTELEAQLKQAKAEGARHIMIATDGVFSMDGVIANLKRVCDLADEYQALVMVDDSHAVGVIGANGRGTHEYCEVMDRV
DIITGTLGKALGGASGGYTAGRKELVEWLRQRSRPYLFSNSLAPAIVAASIEVLSLLEEGGALRDRLWANARLFREKMSA
AGFTLAGADHAIIPVMLGEAKLAQDFANALLKEGIYVTGFFFPVVPKGQARIRTQMSAGHTTEQVERAIEAFVRIGKQLN
VIA
>Mature_402_residues
SLPCNKHPFYQQLEQQLDTTRTEGLYKNERVITSAQQANIAVADGSRVINFCANNYLGLANHPKLIAAAKAGMDSHGFGM
ASVRFICGTQDIHKELEHKLASFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANN
DMTELEAQLKQAKAEGARHIMIATDGVFSMDGVIANLKRVCDLADEYQALVMVDDSHAVGVIGANGRGTHEYCEVMDRVD
IITGTLGKALGGASGGYTAGRKELVEWLRQRSRPYLFSNSLAPAIVAASIEVLSLLEEGGALRDRLWANARLFREKMSAA
GFTLAGADHAIIPVMLGEAKLAQDFANALLKEGIYVTGFFFPVVPKGQARIRTQMSAGHTTEQVERAIEAFVRIGKQLNV
IA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=386, Percent_Identity=56.9948186528497, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI284448556, Length=412, Percent_Identity=53.6407766990291, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI83977442, Length=361, Percent_Identity=36.2880886426593, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI83977440, Length=361, Percent_Identity=36.2880886426593, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI83977444, Length=361, Percent_Identity=36.2880886426593, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4502025, Length=398, Percent_Identity=32.4120603015075, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI40316939, Length=398, Percent_Identity=32.4120603015075, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI119220554, Length=369, Percent_Identity=34.1463414634146, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4758668, Length=369, Percent_Identity=34.6883468834688, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI5454084, Length=380, Percent_Identity=27.1052631578947, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1790046, Length=394, Percent_Identity=84.7715736040609, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1786993, Length=352, Percent_Identity=34.375, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI71994529, Length=396, Percent_Identity=55.8080808080808, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32566772, Length=426, Percent_Identity=29.1079812206573, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560912, Length=399, Percent_Identity=32.0802005012531, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17560914, Length=399, Percent_Identity=32.0802005012531, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71982617, Length=372, Percent_Identity=25.8064516129032, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71982625, Length=372, Percent_Identity=25.8064516129032, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6320438, Length=383, Percent_Identity=32.6370757180157, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6320267, Length=371, Percent_Identity=30.188679245283, Blast_Score=175, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6323954, Length=265, Percent_Identity=29.811320754717, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24662918, Length=390, Percent_Identity=57.1794871794872, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17137420, Length=355, Percent_Identity=33.2394366197183, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI17136286, Length=372, Percent_Identity=33.3333333333333, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24653280, Length=332, Percent_Identity=27.4096385542169, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653276, Length=332, Percent_Identity=27.4096385542169, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653278, Length=332, Percent_Identity=27.4096385542169, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43770; Mature: 43638

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPCNKHPFYQQLEQQLDTTRTEGLYKNERVITSAQQANIAVADGSRVINFCANNYLGL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCEEEEECCHHHHHHHHCCCCCC
ANHPKLIAAAKAGMDSHGFGMASVRFICGTQDIHKELEHKLASFLGMEDAILYSSCFDAN
CCCCCCHHHHHCCCCCCCCCHHHHEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
GGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTELEAQLKQAKAEGARH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
IMIATDGVFSMDGVIANLKRVCDLADEYQALVMVDDSHAVGVIGANGRGTHEYCEVMDRV
EEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHH
DIITGTLGKALGGASGGYTAGRKELVEWLRQRSRPYLFSNSLAPAIVAASIEVLSLLEEG
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHC
GALRDRLWANARLFREKMSAAGFTLAGADHAIIPVMLGEAKLAQDFANALLKEGIYVTGF
CHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEE
FFPVVPKGQARIRTQMSAGHTTEQVERAIEAFVRIGKQLNVIA
EEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLPCNKHPFYQQLEQQLDTTRTEGLYKNERVITSAQQANIAVADGSRVINFCANNYLGL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCEEEEECCHHHHHHHHCCCCCC
ANHPKLIAAAKAGMDSHGFGMASVRFICGTQDIHKELEHKLASFLGMEDAILYSSCFDAN
CCCCCCHHHHHCCCCCCCCCHHHHEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
GGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTELEAQLKQAKAEGARH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
IMIATDGVFSMDGVIANLKRVCDLADEYQALVMVDDSHAVGVIGANGRGTHEYCEVMDRV
EEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHH
DIITGTLGKALGGASGGYTAGRKELVEWLRQRSRPYLFSNSLAPAIVAASIEVLSLLEEG
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHC
GALRDRLWANARLFREKMSAAGFTLAGADHAIIPVMLGEAKLAQDFANALLKEGIYVTGF
CHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEE
FFPVVPKGQARIRTQMSAGHTTEQVERAIEAFVRIGKQLNVIA
EEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]