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The map label for this gene is pldA [H]

Identifier: 157960489

GI number: 157960489

Start: 819752

End: 820717

Strand: Reverse

Name: pldA [H]

Synonym: Spea_0661

Alternate gene names: 157960489

Gene position: 820717-819752 (Counterclockwise)

Preceding gene: 157960496

Following gene: 157960488

Centisome position: 15.86

GC content: 45.24

Gene sequence:

>966_bases
ATGAACAAATTAGCTGCGCGACTGTTAGCCATAGGCTTGTGCTGCTCTTTTAACCTTTATGCTCATAGTGAGACCCAAAC
TAACAAGGGTGAAGAAAGCGAAACTGAAGCGAGTGATTCCGAGATTGAAGTGAAGCCCAAGCAAGAGGTGTCTTTGGTTG
ATAAACGGATCTTGGAAGAAAGCGCAACTGATGATCTGCCTTATGTCATCACCCCCCATAAGGTGAATTATATTCTCCCC
GTGACTTACAGTGCCTCGCCCAATATGGCACCTTTTGCAGAAGAAGCAGCCGAGCATCCGTTTACCTTAGATAATATGGA
AGCCAAGTTTCAAATCAGCTTTAAGTTTCCACTTTGGCATAACGTCTTTGGCGATAATGGCCACTTGTTTGCGGCCTATA
CCAATCAGTCCTATTGGCAGGTGTACAACAAAGAGATCTCCTCGCCATTTAGAGAGACTAACCATGAGCCCGAGATCTTT
ATGATCTTTACCAACGACTGGCAACTTGGACCACTGAAAAATTCGTTTTGGGGTTTTGGTGCCGTGCATCAATCAAACGG
TCAGTCTGGCTCGCGTTCACGCAGCTGGAACCGTTTATACGGCACCATGGTATTTGATAACGGCCCCTTCGCACTAGAAG
CTAAGGCATGGTGGCGCATACCTGAAGATGAAAAGGAAACCCCTGAGTCACCCAAAGGCGATGATAACCCTGATATCACC
GACTATATGGGCAATCTTGAACTCACTGGCGTATACGGCTTGGGCGAACACAGGTTCACCCTGATGGTTCGTAACTATAT
TCAAAAGCCTAACTATGGCGCACTTGAATTCACCTGGAGCTACCCAATCTTGGGTAACTTAAGGCTTTATACTCAATATT
TTAATGGCTACGGTGAAAGTTTAATCGACTACAATGTACATAATCAGCGCATAGGTATCGGGGTTTCGATTAACGACATC
TTATAA

Upstream 100 bases:

>100_bases
CGTCCAGTTACTAATTTGCTGAATAACTGTTAATACGACAGGCGGCTAGGTAGAATCTGCCACATAAGAAGAAACAGCTT
AAATAAATTCAGGGAATACG

Downstream 100 bases:

>100_bases
TCGACTAGGTTAGCTCGATATCAAAAGCGTCTTTAGCAGGCTAAAAGTATAAGCTTTCAGCCTGCCTTAACAAGGATGGT
TATGACTTTGAGCTATAAAA

Product: phospholipase A(1)

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNKLAARLLAIGLCCSFNLYAHSETQTNKGEESETEASDSEIEVKPKQEVSLVDKRILEESATDDLPYVITPHKVNYILP
VTYSASPNMAPFAEEAAEHPFTLDNMEAKFQISFKFPLWHNVFGDNGHLFAAYTNQSYWQVYNKEISSPFRETNHEPEIF
MIFTNDWQLGPLKNSFWGFGAVHQSNGQSGSRSRSWNRLYGTMVFDNGPFALEAKAWWRIPEDEKETPESPKGDDNPDIT
DYMGNLELTGVYGLGEHRFTLMVRNYIQKPNYGALEFTWSYPILGNLRLYTQYFNGYGESLIDYNVHNQRIGIGVSINDI
L

Sequences:

>Translated_321_residues
MNKLAARLLAIGLCCSFNLYAHSETQTNKGEESETEASDSEIEVKPKQEVSLVDKRILEESATDDLPYVITPHKVNYILP
VTYSASPNMAPFAEEAAEHPFTLDNMEAKFQISFKFPLWHNVFGDNGHLFAAYTNQSYWQVYNKEISSPFRETNHEPEIF
MIFTNDWQLGPLKNSFWGFGAVHQSNGQSGSRSRSWNRLYGTMVFDNGPFALEAKAWWRIPEDEKETPESPKGDDNPDIT
DYMGNLELTGVYGLGEHRFTLMVRNYIQKPNYGALEFTWSYPILGNLRLYTQYFNGYGESLIDYNVHNQRIGIGVSINDI
L
>Mature_321_residues
MNKLAARLLAIGLCCSFNLYAHSETQTNKGEESETEASDSEIEVKPKQEVSLVDKRILEESATDDLPYVITPHKVNYILP
VTYSASPNMAPFAEEAAEHPFTLDNMEAKFQISFKFPLWHNVFGDNGHLFAAYTNQSYWQVYNKEISSPFRETNHEPEIF
MIFTNDWQLGPLKNSFWGFGAVHQSNGQSGSRSRSWNRLYGTMVFDNGPFALEAKAWWRIPEDEKETPESPKGDDNPDIT
DYMGNLELTGVYGLGEHRFTLMVRNYIQKPNYGALEFTWSYPILGNLRLYTQYFNGYGESLIDYNVHNQRIGIGVSINDI
L

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=286, Percent_Identity=37.7622377622378, Blast_Score=187, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 36719; Mature: 36719

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLAARLLAIGLCCSFNLYAHSETQTNKGEESETEASDSEIEVKPKQEVSLVDKRILEE
CCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
SATDDLPYVITPHKVNYILPVTYSASPNMAPFAEEAAEHPFTLDNMEAKFQISFKFPLWH
CCCCCCCEEECCCCEEEEEEEEECCCCCCCCCHHHHHCCCEEECCCCEEEEEEEECCHHH
NVFGDNGHLFAAYTNQSYWQVYNKEISSPFRETNHEPEIFMIFTNDWQLGPLKNSFWGFG
HCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHCCCCCEEEEEEECCEEECCCCCCCCCCC
AVHQSNGQSGSRSRSWNRLYGTMVFDNGPFALEAKAWWRIPEDEKETPESPKGDDNPDIT
EEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEHEEECCCCCCCCCCCCCCCCCCCCHH
DYMGNLELTGVYGLGEHRFTLMVRNYIQKPNYGALEFTWSYPILGNLRLYTQYFNGYGES
HHCCCEEEEEEEECCCCEEEEEEHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCC
LIDYNVHNQRIGIGVSINDIL
EEEECCCCCEEEEEEEHHCCC
>Mature Secondary Structure
MNKLAARLLAIGLCCSFNLYAHSETQTNKGEESETEASDSEIEVKPKQEVSLVDKRILEE
CCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
SATDDLPYVITPHKVNYILPVTYSASPNMAPFAEEAAEHPFTLDNMEAKFQISFKFPLWH
CCCCCCCEEECCCCEEEEEEEEECCCCCCCCCHHHHHCCCEEECCCCEEEEEEEECCHHH
NVFGDNGHLFAAYTNQSYWQVYNKEISSPFRETNHEPEIFMIFTNDWQLGPLKNSFWGFG
HCCCCCCEEEEEECCCHHHHHHHHHHCCHHHHCCCCCEEEEEEECCEEECCCCCCCCCCC
AVHQSNGQSGSRSRSWNRLYGTMVFDNGPFALEAKAWWRIPEDEKETPESPKGDDNPDIT
EEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEHEEECCCCCCCCCCCCCCCCCCCCHH
DYMGNLELTGVYGLGEHRFTLMVRNYIQKPNYGALEFTWSYPILGNLRLYTQYFNGYGES
HHCCCEEEEEEEECCCCEEEEEEHHHHCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCC
LIDYNVHNQRIGIGVSINDIL
EEEECCCCCEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8300539 [H]