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The map label for this gene is hisH [H]

Identifier: 145296059

GI number: 145296059

Start: 2178311

End: 2178946

Strand: Reverse

Name: hisH [H]

Synonym: cgR_1981

Alternate gene names: 145296059

Gene position: 2178946-2178311 (Counterclockwise)

Preceding gene: 145296060

Following gene: 145296058

Centisome position: 65.75

GC content: 52.83

Gene sequence:

>636_bases
ATGACCAAAACTGTCGCCCTTCTCGACTACGGATCTGGAAACCTTCGTTCTGCTCAACGCGCACTAGAGCGTGCCGGTGC
AGAAGTTATCGTGAGCTCCGATCCAGAAGTTTGCACCAACGCTGATGGCCTCCTAGTTCCTGGGGTGGGCGCATTTGATG
CCTGCATGAAGGGTTTGAAAAACGTCTTCGGACATCGGATTATCGGACAGCGTCTTGCTGGTGGGCGTCCAGTGATGGGT
ATTTGTGTGGGCATGCAGATCCTGTTTGATGAAGGCGATGAGCATGGCATTAAGTCAGCTGGTTGTGGCGAGTGGCCTGG
CAAGGTGGAACGCCTCCAGGCGGAGATCCTGCCTCACATGGGGTGGAACACACTTGAAATGCCTACCAACTCACCAATGT
TTGAGGGAATTTCACCTGATGAGCGTTTCTACTTCGTGCACTCCTATGGTGTGCGCAAGTGGACGTTGGAAACCGACGAT
CTGACCATACCTCCAGAGGTTGTGTGGGCGAAGCACGAAAATGATCGTTTTGTGGCTGCTGTGGAAAACGGCACGCTGTG
GGCTACTCAATTCCACCCAGAAAAATCAGGTGACGCAGGCGCAAAGCTACTGCGAAACTGGATCAACTACATCTAA

Upstream 100 bases:

>100_bases
ATGGCAGCTTGGCCAGTGTGGTGGATATAAAACCCTTTTGGGGAGAAAGAAACTCGACTGCGGTTCTTGATCCTGAAAGC
ACGTGACATAAACTATCGGC

Downstream 100 bases:

>100_bases
CAGATAGGATCAATATTCATGACCTTCACTATTCTTCCTGCAGTCGATGTAGTTAACGGACAAGCAGTTCGCCTAGATCA
GGGCGAGGCCGGCACTGAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLKNVFGHRIIGQRLAGGRPVMG
ICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHMGWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDD
LTIPPEVVWAKHENDRFVAAVENGTLWATQFHPEKSGDAGAKLLRNWINYI

Sequences:

>Translated_211_residues
MTKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLKNVFGHRIIGQRLAGGRPVMG
ICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHMGWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDD
LTIPPEVVWAKHENDRFVAAVENGTLWATQFHPEKSGDAGAKLLRNWINYI
>Mature_210_residues
TKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLKNVFGHRIIGQRLAGGRPVMGI
CVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHMGWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDDL
TIPPEVVWAKHENDRFVAAVENGTLWATQFHPEKSGDAGAKLLRNWINYI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=37.0192307692308, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=35.5140186915888, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23186; Mature: 23054

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLK
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHCCCCCEEECCCCHHHHHHHHHH
NVFGHRIIGQRLAGGRPVMGICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHM
HHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDDLTIPPEVVWAKHENDRFVAA
CCCEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCEEEEECCCCEEEEE
VENGTLWATQFHPEKSGDAGAKLLRNWINYI
EECCEEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKTVALLDYGSGNLRSAQRALERAGAEVIVSSDPEVCTNADGLLVPGVGAFDACMKGLK
CCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCHHCCCCCEEECCCCHHHHHHHHHH
NVFGHRIIGQRLAGGRPVMGICVGMQILFDEGDEHGIKSAGCGEWPGKVERLQAEILPHM
HHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
GWNTLEMPTNSPMFEGISPDERFYFVHSYGVRKWTLETDDLTIPPEVVWAKHENDRFVAA
CCCEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCEEEEECCCCEEEEE
VENGTLWATQFHPEKSGDAGAKLLRNWINYI
EECCEEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]