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The map label for this gene is hisA

Identifier: 145296058

GI number: 145296058

Start: 2177552

End: 2178292

Strand: Reverse

Name: hisA

Synonym: cgR_1980

Alternate gene names: 145296058

Gene position: 2178292-2177552 (Counterclockwise)

Preceding gene: 145296059

Following gene: 145296057

Centisome position: 65.73

GC content: 52.63

Gene sequence:

>741_bases
ATGACCTTCACTATTCTTCCTGCAGTCGATGTAGTTAACGGACAAGCAGTTCGCCTAGATCAGGGCGAGGCCGGCACTGA
AAAATCTTATGGCACCCCTTTGGAATCCGCACTGAAGTGGCAGGAGCAGGGTGCAAAGTGGTTGCACTTTGTGGACCTGG
ACGCAGCGTTCAACCGTGGTTCCAATCATGAGATGATGGCGGAAATTGTCGGCAAGCTCGATGTTGATGTGGAGCTCACT
GGCGGTATCCGTGATGATGAGTCTCTGGAGCGCGCGCTGGCAACCGGTGCACGTCGTGTAAACATTGGTACCGCTGCTCT
GGAGAAGCCAGAGTGGATTGCTTCTGCGATTCAACGCTATGGCGAGAAGATTGCTGTCGATATCGCTGTGCGTTTGGAAG
ATGGTGAATGGCGCACCCGTGGAAACGGTTGGGTCTCCGATGGTGGCGATCTGTGGGAAGTTCTCGAGCGTTTGGATTCC
CAAGGTTGTGCACGTTTCGTGGTTACCGATGTGTCCAAGGACGGCACCTTGAGTGGTCCAAATGTTGAGCTGCTGCGTGA
GGTTGCTGCAGCTACAGACGCACCTATCGTGGCATCTGGTGGAATTTCTGTTTTGGAAGATGTTTTGGAACTAGCCAAGT
ACCAGGATGAGGGCATTGATTCCGTCATCATTGGCAAGGCACTTTATGAGCACAAGTTCACCCTCGAAGAGGCTTTGGCT
GCAGTAGAAAAGCTCGGTTAA

Upstream 100 bases:

>100_bases
GCTGTGGGCTACTCAATTCCACCCAGAAAAATCAGGTGACGCAGGCGCAAAGCTACTGCGAAACTGGATCAACTACATCT
AACAGATAGGATCAATATTC

Downstream 100 bases:

>100_bases
TACATGGATGCTCGTGGGATGTTGGCCATTGCGGAGGCCGTTGTAGATGATGCCGAAGCCCTCTTCATGCAGGGCTTCGG
AGCTGCTCCTGCCCATATGA

Product: phosphoribosyl isomerase A

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTFTILPAVDVVNGQAVRLDQGEAGTEKSYGTPLESALKWQEQGAKWLHFVDLDAAFNRGSNHEMMAEIVGKLDVDVELT
GGIRDDESLERALATGARRVNIGTAALEKPEWIASAIQRYGEKIAVDIAVRLEDGEWRTRGNGWVSDGGDLWEVLERLDS
QGCARFVVTDVSKDGTLSGPNVELLREVAAATDAPIVASGGISVLEDVLELAKYQDEGIDSVIIGKALYEHKFTLEEALA
AVEKLG

Sequences:

>Translated_246_residues
MTFTILPAVDVVNGQAVRLDQGEAGTEKSYGTPLESALKWQEQGAKWLHFVDLDAAFNRGSNHEMMAEIVGKLDVDVELT
GGIRDDESLERALATGARRVNIGTAALEKPEWIASAIQRYGEKIAVDIAVRLEDGEWRTRGNGWVSDGGDLWEVLERLDS
QGCARFVVTDVSKDGTLSGPNVELLREVAAATDAPIVASGGISVLEDVLELAKYQDEGIDSVIIGKALYEHKFTLEEALA
AVEKLG
>Mature_245_residues
TFTILPAVDVVNGQAVRLDQGEAGTEKSYGTPLESALKWQEQGAKWLHFVDLDAAFNRGSNHEMMAEIVGKLDVDVELTG
GIRDDESLERALATGARRVNIGTAALEKPEWIASAIQRYGEKIAVDIAVRLEDGEWRTRGNGWVSDGGDLWEVLERLDSQ
GCARFVVTDVSKDGTLSGPNVELLREVAAATDAPIVASGGISVLEDVLELAKYQDEGIDSVIIGKALYEHKFTLEEALAA
VEKLG

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=35.1020408163265, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788336, Length=253, Percent_Identity=24.5059288537549, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_CORGL (O68602)

Other databases:

- EMBL:   AF051846
- EMBL:   BA000036
- EMBL:   BX927154
- PIR:   JE0213
- RefSeq:   NP_601295.1
- RefSeq:   YP_226333.1
- ProteinModelPortal:   O68602
- GeneID:   1020047
- GeneID:   3344802
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg2299
- KEGG:   cgl:NCgl2015
- HOGENOM:   HBG541613
- OMA:   CARYVVT
- PhylomeDB:   O68602
- ProtClustDB:   PRK14024
- BioCyc:   CGLU196627:CG2299-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR010188
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR01919

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26609; Mature: 26478

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: ACT_SITE 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTILPAVDVVNGQAVRLDQGEAGTEKSYGTPLESALKWQEQGAKWLHFVDLDAAFNRG
CEEEEEECEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHCCC
SNHEMMAEIVGKLDVDVELTGGIRDDESLERALATGARRVNIGTAALEKPEWIASAIQRY
CCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHHHH
GEKIAVDIAVRLEDGEWRTRGNGWVSDGGDLWEVLERLDSQGCARFVVTDVSKDGTLSGP
CCEEEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
NVELLREVAAATDAPIVASGGISVLEDVLELAKYQDEGIDSVIIGKALYEHKFTLEEALA
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AVEKLG
HHHHCC
>Mature Secondary Structure 
TFTILPAVDVVNGQAVRLDQGEAGTEKSYGTPLESALKWQEQGAKWLHFVDLDAAFNRG
EEEEEECEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHCCC
SNHEMMAEIVGKLDVDVELTGGIRDDESLERALATGARRVNIGTAALEKPEWIASAIQRY
CCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHHHH
GEKIAVDIAVRLEDGEWRTRGNGWVSDGGDLWEVLERLDSQGCARFVVTDVSKDGTLSGP
CCEEEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
NVELLREVAAATDAPIVASGGISVLEDVLELAKYQDEGIDSVIIGKALYEHKFTLEEALA
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AVEKLG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9647764; 12948626