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The map label for this gene is thiE [H]

Identifier: 145296002

GI number: 145296002

Start: 2114314

End: 2114979

Strand: Direct

Name: thiE [H]

Synonym: cgR_1926

Alternate gene names: 145296002

Gene position: 2114314-2114979 (Clockwise)

Preceding gene: 145296000

Following gene: 145296003

Centisome position: 63.8

GC content: 56.31

Gene sequence:

>666_bases
GTGTTTGAAAATCGTTTTGACCTGCGTTGTTATGTTGTGACTGGCGCGGGCTCGGTGGATGAGGTTGTACACACTGCGTC
TGCCGCGGCTCGTGGTGGCGCGGGTGTGGTGCAGGTGCGTTCAAAGCCTATTTCGCCAGAAGCGATGAGGGAGTTGGCAT
CAAAGGTTGCGCTTGAGGTTGCGCGGTGCAGCCCAACAACGAGGGTGCTTATCGACGACCACCTCCACGTTGCTTCTTCC
TTAATGCGCGAAGGACTCCCGATTCATGGTGTGCATCTTGGGCAGGATGATGTGTCGGTGCTTGAGGCTCGTGAGTTGTT
GGGACCTGAGGCGATCATTGGGTTGACTACTGGAACCCTAGAACTTGTGGCGGCGGCGAATGAGCTGTCCGATGTGTTGG
ATTACATCGGTGCTGGGCCGTTTCGGAAGACTCCCACCAAGGATTCAGGTCGGCCACCGATTGGCCTTGCGGGTTATCCC
CCTTTGGTGGAATTGTCCAAGGTGCCGATCGTTGCGATTGGTGATGTCACCCCTGCCGATGTGCGCGCTCTCAGCGCAAC
CGGTGTGGCTGGCGTTGCCATGGTGCGGGCTTTTTCTGAATCTGATGATCCACAGCAGGTCGCTGAAAATGTGGTGGCCA
ACTTTGAATTAGGAAGGCTCTCATGA

Upstream 100 bases:

>100_bases
GTGCCCAATGCATTGGGCTGAGATTGCGCGCTGTTGCTGCGCGGGACCGTTCGAACCTGTCTGGTTAACACCAGCGAAGG
AAGCGAGGATTGATTGTCCC

Downstream 100 bases:

>100_bases
AAAAGCATGCGATTATTATCGGCGGCGGAATCATTGGGTTGACCACCTGCTTTGAATTCCAGGAGGCTGGTTACCAAGTC
ACGTTGATTGATCCTGACCC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MFENRFDLRCYVVTGAGSVDEVVHTASAAARGGAGVVQVRSKPISPEAMRELASKVALEVARCSPTTRVLIDDHLHVASS
LMREGLPIHGVHLGQDDVSVLEARELLGPEAIIGLTTGTLELVAAANELSDVLDYIGAGPFRKTPTKDSGRPPIGLAGYP
PLVELSKVPIVAIGDVTPADVRALSATGVAGVAMVRAFSESDDPQQVAENVVANFELGRLS

Sequences:

>Translated_221_residues
MFENRFDLRCYVVTGAGSVDEVVHTASAAARGGAGVVQVRSKPISPEAMRELASKVALEVARCSPTTRVLIDDHLHVASS
LMREGLPIHGVHLGQDDVSVLEARELLGPEAIIGLTTGTLELVAAANELSDVLDYIGAGPFRKTPTKDSGRPPIGLAGYP
PLVELSKVPIVAIGDVTPADVRALSATGVAGVAMVRAFSESDDPQQVAENVVANFELGRLS
>Mature_221_residues
MFENRFDLRCYVVTGAGSVDEVVHTASAAARGGAGVVQVRSKPISPEAMRELASKVALEVARCSPTTRVLIDDHLHVASS
LMREGLPIHGVHLGQDDVSVLEARELLGPEAIIGLTTGTLELVAAANELSDVLDYIGAGPFRKTPTKDSGRPPIGLAGYP
PLVELSKVPIVAIGDVTPADVRALSATGVAGVAMVRAFSESDDPQQVAENVVANFELGRLS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23099; Mature: 23099

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENRFDLRCYVVTGAGSVDEVVHTASAAARGGAGVVQVRSKPISPEAMRELASKVALEV
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
ARCSPTTRVLIDDHLHVASSLMREGLPIHGVHLGQDDVSVLEARELLGPEAIIGLTTGTL
HHCCCCEEEEEECHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEECCCHH
ELVAAANELSDVLDYIGAGPFRKTPTKDSGRPPIGLAGYPPLVELSKVPIVAIGDVTPAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHH
VRALSATGVAGVAMVRAFSESDDPQQVAENVVANFELGRLS
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFENRFDLRCYVVTGAGSVDEVVHTASAAARGGAGVVQVRSKPISPEAMRELASKVALEV
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
ARCSPTTRVLIDDHLHVASSLMREGLPIHGVHLGQDDVSVLEARELLGPEAIIGLTTGTL
HHCCCCEEEEEECHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEECCCHH
ELVAAANELSDVLDYIGAGPFRKTPTKDSGRPPIGLAGYPPLVELSKVPIVAIGDVTPAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHH
VRALSATGVAGVAMVRAFSESDDPQQVAENVVANFELGRLS
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]