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The map label for this gene is thiO/thiG [H]

Identifier: 145296003

GI number: 145296003

Start: 2114976

End: 2116100

Strand: Direct

Name: thiO/thiG [H]

Synonym: cgR_1927

Alternate gene names: 145296003

Gene position: 2114976-2116100 (Clockwise)

Preceding gene: 145296002

Following gene: 145296004

Centisome position: 63.82

GC content: 55.91

Gene sequence:

>1125_bases
ATGAAAAAGCATGCGATTATTATCGGCGGCGGAATCATTGGGTTGACCACCTGCTTTGAATTCCAGGAGGCTGGTTACCA
AGTCACGTTGATTGATCCTGACCCAATTTCCGGTGCGACTCATCATGCCGGCGGAATGTTGGCCCCCACCGCGGAGGTGC
AGTATCAGCAACATGATCTGATTTTGTTGATGAAGGAATCCGCTCGCCTATACCCAGACCTCCTCCAAAGAGTGTCTAAA
TACACGGACTTGCCCACTGGGTATCGCACTGATGGAACCCTTCTTATCGGTGCGGACCGCGCGGATGGCATGCATGTTGC
GGAGTTGATTAAGTACATGCATTTGAAGGATTTGCCTGTCGATGTGTTGACTACTCGTCAGGCAAGGGCTGCGGAGCCAG
CGTTGTCGCCGAGGATTTCCAAGGTGGTCAGCATTGATGGGGATCATCAGCTGTCGCCGCGTCTGTATGCCCGGGCGTTA
TTGGATGCCATTTTGCAACGCGGTGCGGAGCATATTGCCGACGCTGTGATCTCTATTGATGGCCACGATCCGTGTTTGAC
CGTGACGATGAATTCGGGGGTGAGGGTTGCGTCGAAAAGCGTTGTTGTTTTGGCGGCGGGCCTGGGCGCCGCAAGCATTC
CCGGCTGGTTTGAGGGCGCGAACCCATTGCAGTTGAGGCCGGTGTACGGCGATATTGTGCGCGTGCGCGTGCCGGAGCGA
CTGCAGCCGATGGTCACCAAGGTGGTGCGCGGGTTTGTGGAAGATCGTCAGATTTATATCATTCCGCGTACCGATGGCAC
CCTCGCGATCGGCGCGACAAGCCGTGAGGATCACCCGCAACCTCGAACGGGCGCAGTGCATGATTTGCTACGCGATGCTA
TCCGTTTGATTCCGGGCATTGAAGAAACCGAATTTATCGAAGTCACCTGCGGCGCCCGCCCCGGCACCCCGGATGACCTG
CCGTACCTGGGATGGGTTGGATCCAATGTGATTGCGTCCACAGGATATTTCCGCCACGGAATTTTGCTGTCAGCCCTTGG
TGCGCGCGCTGCCGTTGATATGGTAACCAACCAGCCACTGTTCCCCACTCTTGATGTGTGCGATCCGTTTCGCCACCAAA
TTTAA

Upstream 100 bases:

>100_bases
GTGTGGCTGGCGTTGCCATGGTGCGGGCTTTTTCTGAATCTGATGATCCACAGCAGGTCGCTGAAAATGTGGTGGCCAAC
TTTGAATTAGGAAGGCTCTC

Downstream 100 bases:

>100_bases
GGATTTTTCACAAGTGATTACCTACACCTTCAATGGCACATCTATGCGCTCTTCCGAATTGACCGTGGAGCAATTGGTCC
ACCAAGAAATCGGCCACGAC

Product: hypothetical protein

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKKHAIIIGGGIIGLTTCFEFQEAGYQVTLIDPDPISGATHHAGGMLAPTAEVQYQQHDLILLMKESARLYPDLLQRVSK
YTDLPTGYRTDGTLLIGADRADGMHVAELIKYMHLKDLPVDVLTTRQARAAEPALSPRISKVVSIDGDHQLSPRLYARAL
LDAILQRGAEHIADAVISIDGHDPCLTVTMNSGVRVASKSVVVLAAGLGAASIPGWFEGANPLQLRPVYGDIVRVRVPER
LQPMVTKVVRGFVEDRQIYIIPRTDGTLAIGATSREDHPQPRTGAVHDLLRDAIRLIPGIEETEFIEVTCGARPGTPDDL
PYLGWVGSNVIASTGYFRHGILLSALGARAAVDMVTNQPLFPTLDVCDPFRHQI

Sequences:

>Translated_374_residues
MKKHAIIIGGGIIGLTTCFEFQEAGYQVTLIDPDPISGATHHAGGMLAPTAEVQYQQHDLILLMKESARLYPDLLQRVSK
YTDLPTGYRTDGTLLIGADRADGMHVAELIKYMHLKDLPVDVLTTRQARAAEPALSPRISKVVSIDGDHQLSPRLYARAL
LDAILQRGAEHIADAVISIDGHDPCLTVTMNSGVRVASKSVVVLAAGLGAASIPGWFEGANPLQLRPVYGDIVRVRVPER
LQPMVTKVVRGFVEDRQIYIIPRTDGTLAIGATSREDHPQPRTGAVHDLLRDAIRLIPGIEETEFIEVTCGARPGTPDDL
PYLGWVGSNVIASTGYFRHGILLSALGARAAVDMVTNQPLFPTLDVCDPFRHQI
>Mature_374_residues
MKKHAIIIGGGIIGLTTCFEFQEAGYQVTLIDPDPISGATHHAGGMLAPTAEVQYQQHDLILLMKESARLYPDLLQRVSK
YTDLPTGYRTDGTLLIGADRADGMHVAELIKYMHLKDLPVDVLTTRQARAAEPALSPRISKVVSIDGDHQLSPRLYARAL
LDAILQRGAEHIADAVISIDGHDPCLTVTMNSGVRVASKSVVVLAAGLGAASIPGWFEGANPLQLRPVYGDIVRVRVPER
LQPMVTKVVRGFVEDRQIYIIPRTDGTLAIGATSREDHPQPRTGAVHDLLRDAIRLIPGIEETEFIEVTCGARPGTPDDL
PYLGWVGSNVIASTGYFRHGILLSALGARAAVDMVTNQPLFPTLDVCDPFRHQI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 40603; Mature: 40603

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHAIIIGGGIIGLTTCFEFQEAGYQVTLIDPDPISGATHHAGGMLAPTAEVQYQQHDL
CCCCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEECCCHHCEECCCCE
ILLMKESARLYPDLLQRVSKYTDLPTGYRTDGTLLIGADRADGMHVAELIKYMHLKDLPV
EEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCH
DVLTTRQARAAEPALSPRISKVVSIDGDHQLSPRLYARALLDAILQRGAEHIADAVISID
HHHCCCCHHHCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHEEEEEC
GHDPCLTVTMNSGVRVASKSVVVLAAGLGAASIPGWFEGANPLQLRPVYGDIVRVRVPER
CCCCEEEEEECCCEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEEECCHH
LQPMVTKVVRGFVEDRQIYIIPRTDGTLAIGATSREDHPQPRTGAVHDLLRDAIRLIPGI
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCC
EETEFIEVTCGARPGTPDDLPYLGWVGSNVIASTGYFRHGILLSALGARAAVDMVTNQPL
CCCEEEEEEECCCCCCCCCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FPTLDVCDPFRHQI
CCCHHCCCHHHHCC
>Mature Secondary Structure
MKKHAIIIGGGIIGLTTCFEFQEAGYQVTLIDPDPISGATHHAGGMLAPTAEVQYQQHDL
CCCCEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEECCCHHCEECCCCE
ILLMKESARLYPDLLQRVSKYTDLPTGYRTDGTLLIGADRADGMHVAELIKYMHLKDLPV
EEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCH
DVLTTRQARAAEPALSPRISKVVSIDGDHQLSPRLYARALLDAILQRGAEHIADAVISID
HHHCCCCHHHCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHEEEEEC
GHDPCLTVTMNSGVRVASKSVVVLAAGLGAASIPGWFEGANPLQLRPVYGDIVRVRVPER
CCCCEEEEEECCCEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEEECCHH
LQPMVTKVVRGFVEDRQIYIIPRTDGTLAIGATSREDHPQPRTGAVHDLLRDAIRLIPGI
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCC
EETEFIEVTCGARPGTPDDLPYLGWVGSNVIASTGYFRHGILLSALGARAAVDMVTNQPL
CCCEEEEEEECCCCCCCCCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FPTLDVCDPFRHQI
CCCHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA