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The map label for this gene is mltB [H]

Identifier: 117919442

GI number: 117919442

Start: 1163145

End: 1164209

Strand: Reverse

Name: mltB [H]

Synonym: Shewana3_0993

Alternate gene names: 117919442

Gene position: 1164209-1163145 (Counterclockwise)

Preceding gene: 117919443

Following gene: 117919441

Centisome position: 23.41

GC content: 48.83

Gene sequence:

>1065_bases
ATGCCTATTCTTCGAGCTTGTTTGGCTCCACTGGCAGTACTTTGCCTCAGTTCATATCTCCTTGGTTGCTCGACGGCAAA
CGAGCCTGCGGCCCCCACACCGCAGCCAAGTGCGGCGGTGACCACACAGGCGCCAACGCCAGTATTACCCGAGGCCTTAA
AGGCTGAGTTTATTAAAACCCAAATGGCACAAGGCTTTACCCAAGCCGAAGTTGAAAGTTTTTTAGCAAAGGCCAACTAC
AACCAAGCCGTTATCGATGCTATTTCTCGCCCTTGGGAAGCCAAGCCTTGGCATCAGTATTATCCGATTTTCCTCACCGA
GAAACGCCTCAATGCAGGTCTAGCCTTCTGGAAAAAACACGAGGCAACGATTGCCAAGGCCGCGACAAAGTACCAAGTCG
AGCCACAAATTATTGTGGCCATTATTGGTATCGAAACCTTCTATGGTCAGTACACAGGTAATTATCCGGTAATCGATGCG
CTCTATACCTTAGGGTTTTATTACGAGCCAAGAGCCACTTTCTTTCGCAAGGAATTTGGCGAGCTGATGAAGCTGGTAAA
AGAAGAACACTTAGATATCAATAGCCTCAAGGGTTCCTACGCCGGAGCCATGGGCTTTGGTCAGTTTATTCCTTCAAGCT
ACCGCCACTATGCCGTGGATTTTGATGGCAGCGGTAACCGTGATTTACTCCAGAGTCCAGAGGATGCCATTGGCAGTGTT
GCCAATTATTTCCATGAACACGGCTGGCAGTTGCATGCACCCGTTGCGCTCCCTTTAGTGAATAGCAGTGCCAATGCGCC
TAAAGCCAAAGTGTGGGCTGGTGAGAAGTTGCAGTACAAGGTCTCTGATATTCTCAGCCCAAGCTTATCGCTGGCCACCG
CCCGCGATTTAGATGCCTCGCAAAAGGCCATGCTCATCGAGCTCGAACAAGTGGGTAGCAAGGACTACTGGCTAGGACTC
AATAACTTTTATGTGATCACCCGTTATAACCGTAGCCCTTTGTATTCCATGGCCGTTTATCAATTTAGCCAACAACTGAA
GCAACAACATGACGCTAAAAAATAA

Upstream 100 bases:

>100_bases
GTGGTACTTCCATGCTGACATTGATGACAGGGTTTGGCATTCTTATGAGCATTCATACCCATAGACGTTTTGTAGATAGA
TAATTTTTAGGGACTTTTTA

Downstream 100 bases:

>100_bases
CCTTTTACCGCTCTTGATGCTCGGCTGCTGTTTTGTGCTCGCGGCCTGCTCGAGTTCTAATTCTGGCTCTGCCAGCGAAA
AGAACAAGAACATGGATCCC

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPILRACLAPLAVLCLSSYLLGCSTANEPAAPTPQPSAAVTTQAPTPVLPEALKAEFIKTQMAQGFTQAEVESFLAKANY
NQAVIDAISRPWEAKPWHQYYPIFLTEKRLNAGLAFWKKHEATIAKAATKYQVEPQIIVAIIGIETFYGQYTGNYPVIDA
LYTLGFYYEPRATFFRKEFGELMKLVKEEHLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIGSV
ANYFHEHGWQLHAPVALPLVNSSANAPKAKVWAGEKLQYKVSDILSPSLSLATARDLDASQKAMLIELEQVGSKDYWLGL
NNFYVITRYNRSPLYSMAVYQFSQQLKQQHDAKK

Sequences:

>Translated_354_residues
MPILRACLAPLAVLCLSSYLLGCSTANEPAAPTPQPSAAVTTQAPTPVLPEALKAEFIKTQMAQGFTQAEVESFLAKANY
NQAVIDAISRPWEAKPWHQYYPIFLTEKRLNAGLAFWKKHEATIAKAATKYQVEPQIIVAIIGIETFYGQYTGNYPVIDA
LYTLGFYYEPRATFFRKEFGELMKLVKEEHLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIGSV
ANYFHEHGWQLHAPVALPLVNSSANAPKAKVWAGEKLQYKVSDILSPSLSLATARDLDASQKAMLIELEQVGSKDYWLGL
NNFYVITRYNRSPLYSMAVYQFSQQLKQQHDAKK
>Mature_353_residues
PILRACLAPLAVLCLSSYLLGCSTANEPAAPTPQPSAAVTTQAPTPVLPEALKAEFIKTQMAQGFTQAEVESFLAKANYN
QAVIDAISRPWEAKPWHQYYPIFLTEKRLNAGLAFWKKHEATIAKAATKYQVEPQIIVAIIGIETFYGQYTGNYPVIDAL
YTLGFYYEPRATFFRKEFGELMKLVKEEHLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIGSVA
NYFHEHGWQLHAPVALPLVNSSANAPKAKVWAGEKLQYKVSDILSPSLSLATARDLDASQKAMLIELEQVGSKDYWLGLN
NFYVITRYNRSPLYSMAVYQFSQQLKQQHDAKK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=292, Percent_Identity=39.3835616438356, Blast_Score=195, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 39467; Mature: 39336

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILRACLAPLAVLCLSSYLLGCSTANEPAAPTPQPSAAVTTQAPTPVLPEALKAEFIKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
QMAQGFTQAEVESFLAKANYNQAVIDAISRPWEAKPWHQYYPIFLTEKRLNAGLAFWKKH
HHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCHHHCCHHHHHHH
EATIAKAATKYQVEPQIIVAIIGIETFYGQYTGNYPVIDALYTLGFYYEPRATFFRKEFG
HHHHHHHHHHEECCCEEEEEEEEHHHHHHCCCCCCHHHHHHHHHCEEECCHHHHHHHHHH
ELMKLVKEEHLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIGSV
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHH
ANYFHEHGWQLHAPVALPLVNSSANAPKAKVWAGEKLQYKVSDILSPSLSLATARDLDAS
HHHHHHCCCEEECCEEEEEECCCCCCCCCEEECCCCCEEHHHHHCCCCHHHHHHHCCCCC
QKAMLIELEQVGSKDYWLGLNNFYVITRYNRSPLYSMAVYQFSQQLKQQHDAKK
CHHEEEEHHHCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PILRACLAPLAVLCLSSYLLGCSTANEPAAPTPQPSAAVTTQAPTPVLPEALKAEFIKT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
QMAQGFTQAEVESFLAKANYNQAVIDAISRPWEAKPWHQYYPIFLTEKRLNAGLAFWKKH
HHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCHHHCCHHHHHHH
EATIAKAATKYQVEPQIIVAIIGIETFYGQYTGNYPVIDALYTLGFYYEPRATFFRKEFG
HHHHHHHHHHEECCCEEEEEEEEHHHHHHCCCCCCHHHHHHHHHCEEECCHHHHHHHHHH
ELMKLVKEEHLDINSLKGSYAGAMGFGQFIPSSYRHYAVDFDGSGNRDLLQSPEDAIGSV
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHH
ANYFHEHGWQLHAPVALPLVNSSANAPKAKVWAGEKLQYKVSDILSPSLSLATARDLDAS
HHHHHHCCCEEECCEEEEEECCCCCCCCCEEECCCCCEEHHHHHCCCCHHHHHHHCCCCC
QKAMLIELEQVGSKDYWLGLNNFYVITRYNRSPLYSMAVYQFSQQLKQQHDAKK
CHHEEEEHHHCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]