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The map label for this gene is clpA-1 [H]

Identifier: 116328481

GI number: 116328481

Start: 2129509

End: 2132088

Strand: Reverse

Name: clpA-1 [H]

Synonym: LBL_1829

Alternate gene names: 116328481

Gene position: 2132088-2129509 (Counterclockwise)

Preceding gene: 116328485

Following gene: 116328480

Centisome position: 58.99

GC content: 43.68

Gene sequence:

>2580_bases
ATGAAACTAGATAAACTCACTGCAAAATTAAATGAAGCGTTGCATAACGCACAAGTTTCTGCCGAGAAGTCGGGAAATCC
GGAAATCAGTGAAGAACACATTCTTAGAGAAGTTTTGGTCCAGACCGACGGACTTGTCCCACTTTTGATTTCAAAACTTA
ACCTAAATCCGAAATCCTTTCTCGAAAAAACCGAAGACGCACTGAGGCGGCTTCCGAAAGTCGGAGGAACTTCTTCCGCT
GATGTGGGATTCTCACGTTCCGCAGTTTCTCTTTTGAAAACTGCGGATGAAATCCGTAGGGAACTAAAAGACGAGTATTT
ATCAACGGACCATGTAATTCTCGGGCTTATGAAAAATGGAGTCGGACCGTTGAAGGAGGAATTTTTAAAACTAGGATTAG
AATATTCTAAATTACTTAATATTACTTTGGAGAATAGAAAGGGAAAGGCAATCATGGACGATTCACCGGAAGGAAAAATG
GACGCTCTTGCAAAGTATGCAAAAAATCTTAACGACCTCGCCAAACAGGGAAAACTTGATCCTGTTATCGGAAGAGACGA
AGAGATAAGAAGGACGATTCAAGTCCTCTCCAGAAGAACGAAAAATAACCCGGTTCTTATCGGAGAACCCGGAGTAGGTA
AGACTGCGATCGTAGAAGGACTTGCGAACAAGATCGTACAAGGGGAGGTTCCGGAAGGAATCAGGAATAAAACTCTTTAT
ACCCTGGACCTAGGTTCCATGATCGCGGGAGCCAAATACAGAGGGGAGTTTGAAGACAGATTGAAAGCCCTTCTCGACGA
GGTTAAGGCTTCGGACGGCGAGGTTATTTTATTTATAGACGAAATTCATACTCTTGTAGGCGCCGGCGCAACGGAAGGTG
CATTAGACGCTTCTAATATGCTGAAGCCGATGCTTGCCAGAGGAGAACTCCGATGTATCGGTGCTACCACTCTGAAAGAA
TATCAGAAATACATTGAAAAGGACGCCGCATTGGAAAGAAGGTTTCAACCCGTTTACGTTAAAGAACCTTCCGTCGAGGA
AACGGTTACGATCTTAAGAGGACTCAAAGGACGTTACGAGCTCCATCATGGAATTCGAATTCTTGATTCCGCGTTGATCG
CGGCGGCTACCCTTTCCAATCGGTATATCGTCGACCGTTTCCTTCCCGATAAAGCGGTGGATTTGATCGACGAGGCGTCT
TCTAAGATGAGGATCGAGATCGACTCCATGCCGGAAGAATTGGATCGCGCGAGTAAACGGATTCAATCCTTAAAAATCGA
AAGAGAGGCGTTGAAAAAAGAACAGGATCCCGCTTCGAAAGAAAGATTGAAATCACTCGAAAAGGATCTTGCGGAACAAG
AGCAGAATTTTCAGACTTTGAAGGCGAGATGGGATTTGGAAAAATCCAAAATCTTACGATTGAAACAAATTAAGGAAGAG
ATCGAAAAGTATAAAAACTTAGAAGCGGACGCGGAAAGAAAAGGAGAAATCAATCGAGTCGCCGAAATCCGCTACGGAAA
ACTAGTCGATTTACAAAAAGAATTCGAAGCCGCAAACGAAGAATTAAAAAAACAAGAAGGGGCCTCTCGGCTTTTGAAAG
AAGAAGTCTCCGAAGAAGACATTGCGAACATCGTTTCTCGTTGGACCGGGATTCCCGTATCCAAAATGCTTCAAGGGGAA
AGAGCAAAACTTCTTTTGATGGAAGACGCTTTGAAGACAAGGGTTATCGGCCAGGATCACGCTCTCAAACTTGTATCTGA
AGCGGTACAAAGATCAAGGGCTGGGATTGCGGATCCGAATCGACCGATTGGAACTTTCTTATTCCTGGGACCTACGGGAG
TTGGAAAAACGGAAACCGCAAAGGCTCTCGCCGAATTCTTGTTCGACGATGCAAATGCGATGCATCGAATCGATATGAGC
GAGTATATGGAAGCGCATTCTGTTGCGCGACTTATCGGAGCGCCGCCCGGTTATGTGGGATACGACGAAGGGGGACAACT
CACCGAAGCGGTGAGAAGAAGGCCATATTCTCTGATTCTATTCGACGAAGTTGAAAAAGCGAACCCGGATGTATTCAACA
TTTTTCTTCAAATTTTGGACGAAGGTAGATTAACCGATGGAAAGGGGCGTAACGTTGATTTCAAGAACACGGTGATCATT
CTCACTTCCAATATCGGATCGGACATTTTGGGTTCTTCCGAATATAGCGCCGAAGAAAAAGAAAGATTAGTCGAAGAACG
TTTGAAAAAACAATTCAAACCCGAATTCTTAAACCGAATCGACGAAGTGATTCTATTTCATTCAATTTCAAATTCCGTGA
TTCACAAAATTGCAGACATTCAGTTGGAGGCGTTGAAACAAAAGGCGAAGGAGAACGGTTTGGATGTTCAGTTTACAGAC
GAATTGAAGGACTATGTTTCTAGAGCAGGTTTTGATGCCGAATATGGAGCTAGACCTCTGAAACGTTTGATCCAAAGAGA
AGTTGGCAACTCTCTCAGTCGCTACATTTTAAATGGTAAATTTTCGGACGGACAAAAAGTGACTGTGGATTATAGACAGG
GAAAAGTGATCGTAATTTAA

Upstream 100 bases:

>100_bases
GGGTTGCCTATTAAAATAAGAGTGTCAATATGGCAGAAAAATTTGAGATTTTTAGTATCAGAGAGTCTAAATTCTAAATG
AAAAGTTGGAGAATTAGGGA

Downstream 100 bases:

>100_bases
ACTGGGGCCGATTGTTTATTGTGGAACGTCGAACCCGTCTCAAAACATCAAAATGTAGAAACTACTACATTTTTTATACG
ATAAACAAGTAGGTTCTGAG

Product: endopeptidase Clp, ATP-dependent proteolytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MKLDKLTAKLNEALHNAQVSAEKSGNPEISEEHILREVLVQTDGLVPLLISKLNLNPKSFLEKTEDALRRLPKVGGTSSA
DVGFSRSAVSLLKTADEIRRELKDEYLSTDHVILGLMKNGVGPLKEEFLKLGLEYSKLLNITLENRKGKAIMDDSPEGKM
DALAKYAKNLNDLAKQGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLANKIVQGEVPEGIRNKTLY
TLDLGSMIAGAKYRGEFEDRLKALLDEVKASDGEVILFIDEIHTLVGAGATEGALDASNMLKPMLARGELRCIGATTLKE
YQKYIEKDAALERRFQPVYVKEPSVEETVTILRGLKGRYELHHGIRILDSALIAAATLSNRYIVDRFLPDKAVDLIDEAS
SKMRIEIDSMPEELDRASKRIQSLKIEREALKKEQDPASKERLKSLEKDLAEQEQNFQTLKARWDLEKSKILRLKQIKEE
IEKYKNLEADAERKGEINRVAEIRYGKLVDLQKEFEAANEELKKQEGASRLLKEEVSEEDIANIVSRWTGIPVSKMLQGE
RAKLLLMEDALKTRVIGQDHALKLVSEAVQRSRAGIADPNRPIGTFLFLGPTGVGKTETAKALAEFLFDDANAMHRIDMS
EYMEAHSVARLIGAPPGYVGYDEGGQLTEAVRRRPYSLILFDEVEKANPDVFNIFLQILDEGRLTDGKGRNVDFKNTVII
LTSNIGSDILGSSEYSAEEKERLVEERLKKQFKPEFLNRIDEVILFHSISNSVIHKIADIQLEALKQKAKENGLDVQFTD
ELKDYVSRAGFDAEYGARPLKRLIQREVGNSLSRYILNGKFSDGQKVTVDYRQGKVIVI

Sequences:

>Translated_859_residues
MKLDKLTAKLNEALHNAQVSAEKSGNPEISEEHILREVLVQTDGLVPLLISKLNLNPKSFLEKTEDALRRLPKVGGTSSA
DVGFSRSAVSLLKTADEIRRELKDEYLSTDHVILGLMKNGVGPLKEEFLKLGLEYSKLLNITLENRKGKAIMDDSPEGKM
DALAKYAKNLNDLAKQGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLANKIVQGEVPEGIRNKTLY
TLDLGSMIAGAKYRGEFEDRLKALLDEVKASDGEVILFIDEIHTLVGAGATEGALDASNMLKPMLARGELRCIGATTLKE
YQKYIEKDAALERRFQPVYVKEPSVEETVTILRGLKGRYELHHGIRILDSALIAAATLSNRYIVDRFLPDKAVDLIDEAS
SKMRIEIDSMPEELDRASKRIQSLKIEREALKKEQDPASKERLKSLEKDLAEQEQNFQTLKARWDLEKSKILRLKQIKEE
IEKYKNLEADAERKGEINRVAEIRYGKLVDLQKEFEAANEELKKQEGASRLLKEEVSEEDIANIVSRWTGIPVSKMLQGE
RAKLLLMEDALKTRVIGQDHALKLVSEAVQRSRAGIADPNRPIGTFLFLGPTGVGKTETAKALAEFLFDDANAMHRIDMS
EYMEAHSVARLIGAPPGYVGYDEGGQLTEAVRRRPYSLILFDEVEKANPDVFNIFLQILDEGRLTDGKGRNVDFKNTVII
LTSNIGSDILGSSEYSAEEKERLVEERLKKQFKPEFLNRIDEVILFHSISNSVIHKIADIQLEALKQKAKENGLDVQFTD
ELKDYVSRAGFDAEYGARPLKRLIQREVGNSLSRYILNGKFSDGQKVTVDYRQGKVIVI
>Mature_859_residues
MKLDKLTAKLNEALHNAQVSAEKSGNPEISEEHILREVLVQTDGLVPLLISKLNLNPKSFLEKTEDALRRLPKVGGTSSA
DVGFSRSAVSLLKTADEIRRELKDEYLSTDHVILGLMKNGVGPLKEEFLKLGLEYSKLLNITLENRKGKAIMDDSPEGKM
DALAKYAKNLNDLAKQGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLANKIVQGEVPEGIRNKTLY
TLDLGSMIAGAKYRGEFEDRLKALLDEVKASDGEVILFIDEIHTLVGAGATEGALDASNMLKPMLARGELRCIGATTLKE
YQKYIEKDAALERRFQPVYVKEPSVEETVTILRGLKGRYELHHGIRILDSALIAAATLSNRYIVDRFLPDKAVDLIDEAS
SKMRIEIDSMPEELDRASKRIQSLKIEREALKKEQDPASKERLKSLEKDLAEQEQNFQTLKARWDLEKSKILRLKQIKEE
IEKYKNLEADAERKGEINRVAEIRYGKLVDLQKEFEAANEELKKQEGASRLLKEEVSEEDIANIVSRWTGIPVSKMLQGE
RAKLLLMEDALKTRVIGQDHALKLVSEAVQRSRAGIADPNRPIGTFLFLGPTGVGKTETAKALAEFLFDDANAMHRIDMS
EYMEAHSVARLIGAPPGYVGYDEGGQLTEAVRRRPYSLILFDEVEKANPDVFNIFLQILDEGRLTDGKGRNVDFKNTVII
LTSNIGSDILGSSEYSAEEKERLVEERLKKQFKPEFLNRIDEVILFHSISNSVIHKIADIQLEALKQKAKENGLDVQFTD
ELKDYVSRAGFDAEYGARPLKRLIQREVGNSLSRYILNGKFSDGQKVTVDYRQGKVIVI

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=37.7926421404682, Blast_Score=203, Evalue=6e-52,
Organism=Escherichia coli, GI1788943, Length=864, Percent_Identity=53.125, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=270, Percent_Identity=52.2222222222222, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=717, Percent_Identity=50.9065550906555, Blast_Score=752, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=856, Percent_Identity=41.2383177570093, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96324; Mature: 96324

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDKLTAKLNEALHNAQVSAEKSGNPEISEEHILREVLVQTDGLVPLLISKLNLNPKSF
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
LEKTEDALRRLPKVGGTSSADVGFSRSAVSLLKTADEIRRELKDEYLSTDHVILGLMKNG
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
VGPLKEEFLKLGLEYSKLLNITLENRKGKAIMDDSPEGKMDALAKYAKNLNDLAKQGKLD
CCHHHHHHHHHCCCHHHHEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLANKIVQGEVPEGIRNKTLY
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
TLDLGSMIAGAKYRGEFEDRLKALLDEVKASDGEVILFIDEIHTLVGAGATEGALDASNM
EEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPMLARGELRCIGATTLKEYQKYIEKDAALERRFQPVYVKEPSVEETVTILRGLKGRYE
HHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCHHH
LHHGIRILDSALIAAATLSNRYIVDRFLPDKAVDLIDEASSKMRIEIDSMPEELDRASKR
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
IQSLKIEREALKKEQDPASKERLKSLEKDLAEQEQNFQTLKARWDLEKSKILRLKQIKEE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IEKYKNLEADAERKGEINRVAEIRYGKLVDLQKEFEAANEELKKQEGASRLLKEEVSEED
HHHHHCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IANIVSRWTGIPVSKMLQGERAKLLLMEDALKTRVIGQDHALKLVSEAVQRSRAGIADPN
HHHHHHHHCCCCHHHHHCCCCHHEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
RPIGTFLFLGPTGVGKTETAKALAEFLFDDANAMHRIDMSEYMEAHSVARLIGAPPGYVG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
YDEGGQLTEAVRRRPYSLILFDEVEKANPDVFNIFLQILDEGRLTDGKGRNVDFKNTVII
CCCCCHHHHHHHHCCCEEEEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LTSNIGSDILGSSEYSAEEKERLVEERLKKQFKPEFLNRIDEVILFHSISNSVIHKIADI
EECCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLEALKQKAKENGLDVQFTDELKDYVSRAGFDAEYGARPLKRLIQREVGNSLSRYILNGK
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCC
FSDGQKVTVDYRQGKVIVI
CCCCCEEEEEECCCCEEEC
>Mature Secondary Structure
MKLDKLTAKLNEALHNAQVSAEKSGNPEISEEHILREVLVQTDGLVPLLISKLNLNPKSF
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
LEKTEDALRRLPKVGGTSSADVGFSRSAVSLLKTADEIRRELKDEYLSTDHVILGLMKNG
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
VGPLKEEFLKLGLEYSKLLNITLENRKGKAIMDDSPEGKMDALAKYAKNLNDLAKQGKLD
CCHHHHHHHHHCCCHHHHEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLANKIVQGEVPEGIRNKTLY
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEE
TLDLGSMIAGAKYRGEFEDRLKALLDEVKASDGEVILFIDEIHTLVGAGATEGALDASNM
EEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPMLARGELRCIGATTLKEYQKYIEKDAALERRFQPVYVKEPSVEETVTILRGLKGRYE
HHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCHHH
LHHGIRILDSALIAAATLSNRYIVDRFLPDKAVDLIDEASSKMRIEIDSMPEELDRASKR
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
IQSLKIEREALKKEQDPASKERLKSLEKDLAEQEQNFQTLKARWDLEKSKILRLKQIKEE
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IEKYKNLEADAERKGEINRVAEIRYGKLVDLQKEFEAANEELKKQEGASRLLKEEVSEED
HHHHHCCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IANIVSRWTGIPVSKMLQGERAKLLLMEDALKTRVIGQDHALKLVSEAVQRSRAGIADPN
HHHHHHHHCCCCHHHHHCCCCHHEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
RPIGTFLFLGPTGVGKTETAKALAEFLFDDANAMHRIDMSEYMEAHSVARLIGAPPGYVG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
YDEGGQLTEAVRRRPYSLILFDEVEKANPDVFNIFLQILDEGRLTDGKGRNVDFKNTVII
CCCCCHHHHHHHHCCCEEEEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LTSNIGSDILGSSEYSAEEKERLVEERLKKQFKPEFLNRIDEVILFHSISNSVIHKIADI
EECCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLEALKQKAKENGLDVQFTDELKDYVSRAGFDAEYGARPLKRLIQREVGNSLSRYILNGK
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCC
FSDGQKVTVDYRQGKVIVI
CCCCCEEEEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA