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The map label for this gene is folC [H]

Identifier: 239618104

GI number: 239618104

Start: 1863658

End: 1864989

Strand: Reverse

Name: folC [H]

Synonym: Kole_1736

Alternate gene names: 239618104

Gene position: 1864989-1863658 (Counterclockwise)

Preceding gene: 239618105

Following gene: 239618103

Centisome position: 81.01

GC content: 41.82

Gene sequence:

>1332_bases
GTGACTGTTATGGAATACAGAGAAGCTCTGGAGTACCTTTATTCTTCGAGACCGTACGGAAAAATCAAGTACGGTCTCTT
TCGTATTGAAGAATTGATGGAACGCCTGGGGAACCCTCAAGAAAGCTATCCTACCATACACATAACAGGTACAAACGGGA
AAGGAAGTGTAGCAACGATACTCAAAGGGGTTCTCGAAGCCCATGGATTACACGTTGGTATGAATATATCACCGCATATC
GTTTCCTTCAGGGAGCGAATTCAATTAGACAACAGGTACATAACAGAAGAAGAGGTATGTGAAACCCTGAAAGAAATTCT
GCCAGCAATTGAAACGATGGATAAAAAAGGGCCAGAATATGCCCCGAGTTTCTTTGAGGTTGTAACCGCCATGGCCTTCC
ACTTTTTCAGAAAGAAAAAGGTGGATGTTGCCGTCATCGAAGTTGGCTTAGGCGGTAGATACGATGCAACCAATATCATT
AAGAAACCTCTTGTTTCAGTCATAACAACTGTTTCTCTGGATCACAAAAACATACTGGGGAAGAACGAAGAAAAAATTGC
TATTGAAAAAGCCGGGATCATCAAAGAAGGTGTTCCGGTTGTTTCAGGTGTAACACGTCCTTCCATTCGATATACAATCG
AAGAAAAAGCCGGTGAAAAGAACGCTCCTTCATACTTTCTATGGAAAGATTTTCAGGTTGAGACGAAGGAATTAAAAATC
AATGAAAACGTGTACAATTATTCTGGAGAGGAAACGTACCCCAACCTGGTCCTCTCTTTGAACGGTACCCATCAGGCAAG
AAACCTGGCGGTAGCTATGAAAACCTTAGAGATCGCTTTTAATGGATTGAAAAAGAAGATCGATAATGCCAAACTGCGAG
ACAGCCTCAAAAAAACAAGCTGGCCCGGAAGGTTTGAGGTTTTAACCCATAAAAACAGGAAAATAATCCTTGATGGTGCT
CATAATATCGACGGTGCTTATGCTCTACGGAACAGTCTTGAGATTTACTTCCCCGGTCAAAAGCTCGATATAATTTTCGG
AAGTCTCGATGACAAAGACTACGAAAGCGTGATTTCAATTCTTGCACCTATCTCCGGGAAAGTTGTCGTAACAAAGGTTC
CGAGTCACAGAAGCATAAATCCCGAGCGGGTGCGGGAAATATGGAAAGTGTACCACGGTAATGTTGAGTTTATAACGGAA
CCGGATAGAGCCTTTGAAAAATTTTTCAACAGCACACAAAATACTTTACTCATCACGGGTTCTCTGTATCTGGTGAGTTA
TCTTAGAAATCTAATCGTTGAGGGAGTTGGAGATATTGATAAAAGGCGTTGA

Upstream 100 bases:

>100_bases
TCGAAAGAGCTCCGGAAGAGGTTGTTGAGGAAACAAAAGAAAAACTGAAAGCTGCCAAAAGCAATTGTGATAGGCTCAAA
AAAATTATAGATGATTTGAA

Downstream 100 bases:

>100_bases
AGGAGAAATAACAGAGATATCCGGAAACGAGGTCACACTGAAGGCAGGACAGTTCTATCTGAACATTCTGTGTTCGACCA
ATACAATAAAGGAACTTTCA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTVMEYREALEYLYSSRPYGKIKYGLFRIEELMERLGNPQESYPTIHITGTNGKGSVATILKGVLEAHGLHVGMNISPHI
VSFRERIQLDNRYITEEEVCETLKEILPAIETMDKKGPEYAPSFFEVVTAMAFHFFRKKKVDVAVIEVGLGGRYDATNII
KKPLVSVITTVSLDHKNILGKNEEKIAIEKAGIIKEGVPVVSGVTRPSIRYTIEEKAGEKNAPSYFLWKDFQVETKELKI
NENVYNYSGEETYPNLVLSLNGTHQARNLAVAMKTLEIAFNGLKKKIDNAKLRDSLKKTSWPGRFEVLTHKNRKIILDGA
HNIDGAYALRNSLEIYFPGQKLDIIFGSLDDKDYESVISILAPISGKVVVTKVPSHRSINPERVREIWKVYHGNVEFITE
PDRAFEKFFNSTQNTLLITGSLYLVSYLRNLIVEGVGDIDKRR

Sequences:

>Translated_443_residues
MTVMEYREALEYLYSSRPYGKIKYGLFRIEELMERLGNPQESYPTIHITGTNGKGSVATILKGVLEAHGLHVGMNISPHI
VSFRERIQLDNRYITEEEVCETLKEILPAIETMDKKGPEYAPSFFEVVTAMAFHFFRKKKVDVAVIEVGLGGRYDATNII
KKPLVSVITTVSLDHKNILGKNEEKIAIEKAGIIKEGVPVVSGVTRPSIRYTIEEKAGEKNAPSYFLWKDFQVETKELKI
NENVYNYSGEETYPNLVLSLNGTHQARNLAVAMKTLEIAFNGLKKKIDNAKLRDSLKKTSWPGRFEVLTHKNRKIILDGA
HNIDGAYALRNSLEIYFPGQKLDIIFGSLDDKDYESVISILAPISGKVVVTKVPSHRSINPERVREIWKVYHGNVEFITE
PDRAFEKFFNSTQNTLLITGSLYLVSYLRNLIVEGVGDIDKRR
>Mature_442_residues
TVMEYREALEYLYSSRPYGKIKYGLFRIEELMERLGNPQESYPTIHITGTNGKGSVATILKGVLEAHGLHVGMNISPHIV
SFRERIQLDNRYITEEEVCETLKEILPAIETMDKKGPEYAPSFFEVVTAMAFHFFRKKKVDVAVIEVGLGGRYDATNIIK
KPLVSVITTVSLDHKNILGKNEEKIAIEKAGIIKEGVPVVSGVTRPSIRYTIEEKAGEKNAPSYFLWKDFQVETKELKIN
ENVYNYSGEETYPNLVLSLNGTHQARNLAVAMKTLEIAFNGLKKKIDNAKLRDSLKKTSWPGRFEVLTHKNRKIILDGAH
NIDGAYALRNSLEIYFPGQKLDIIFGSLDDKDYESVISILAPISGKVVVTKVPSHRSINPERVREIWKVYHGNVEFITEP
DRAFEKFFNSTQNTLLITGSLYLVSYLRNLIVEGVGDIDKRR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=349, Percent_Identity=30.6590257879656, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI66932984, Length=349, Percent_Identity=30.6590257879656, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1788654, Length=428, Percent_Identity=28.7383177570093, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=439, Percent_Identity=26.879271070615, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17553148, Length=439, Percent_Identity=26.879271070615, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71984923, Length=439, Percent_Identity=26.879271070615, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323760, Length=442, Percent_Identity=30.9954751131222, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6324815, Length=327, Percent_Identity=31.8042813455657, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6322718, Length=288, Percent_Identity=26.0416666666667, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24641571, Length=301, Percent_Identity=30.8970099667774, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24581568, Length=306, Percent_Identity=27.1241830065359, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 50203; Mature: 50072

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMEYREALEYLYSSRPYGKIKYGLFRIEELMERLGNPQESYPTIHITGTNGKGSVATI
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCHHHHH
LKGVLEAHGLHVGMNISPHIVSFRERIQLDNRYITEEEVCETLKEILPAIETMDKKGPEY
HHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
APSFFEVVTAMAFHFFRKKKVDVAVIEVGLGGRYDATNIIKKPLVSVITTVSLDHKNILG
CHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCC
KNEEKIAIEKAGIIKEGVPVVSGVTRPSIRYTIEEKAGEKNAPSYFLWKDFQVETKELKI
CCCCEEEEECCCCHHCCCCHHCCCCCCCEEEEEHHHCCCCCCCCEEEEECCEEEEEEEEE
NENVYNYSGEETYPNLVLSLNGTHQARNLAVAMKTLEIAFNGLKKKIDNAKLRDSLKKTS
ECCEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
WPGRFEVLTHKNRKIILDGAHNIDGAYALRNSLEIYFPGQKLDIIFGSLDDKDYESVISI
CCCEEEEEEECCCEEEEECCCCCCHHHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHH
LAPISGKVVVTKVPSHRSINPERVREIWKVYHGNVEFITEPDRAFEKFFNSTQNTLLITG
HCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEEH
SLYLVSYLRNLIVEGVGDIDKRR
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVMEYREALEYLYSSRPYGKIKYGLFRIEELMERLGNPQESYPTIHITGTNGKGSVATI
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCHHHHH
LKGVLEAHGLHVGMNISPHIVSFRERIQLDNRYITEEEVCETLKEILPAIETMDKKGPEY
HHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
APSFFEVVTAMAFHFFRKKKVDVAVIEVGLGGRYDATNIIKKPLVSVITTVSLDHKNILG
CHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCC
KNEEKIAIEKAGIIKEGVPVVSGVTRPSIRYTIEEKAGEKNAPSYFLWKDFQVETKELKI
CCCCEEEEECCCCHHCCCCHHCCCCCCCEEEEEHHHCCCCCCCCEEEEECCEEEEEEEEE
NENVYNYSGEETYPNLVLSLNGTHQARNLAVAMKTLEIAFNGLKKKIDNAKLRDSLKKTS
ECCEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
WPGRFEVLTHKNRKIILDGAHNIDGAYALRNSLEIYFPGQKLDIIFGSLDDKDYESVISI
CCCEEEEEEECCCEEEEECCCCCCHHHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHH
LAPISGKVVVTKVPSHRSINPERVREIWKVYHGNVEFITEPDRAFEKFFNSTQNTLLITG
HCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEEH
SLYLVSYLRNLIVEGVGDIDKRR
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]