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The map label for this gene is uvrB

Identifier: 239618098

GI number: 239618098

Start: 1857493

End: 1859496

Strand: Reverse

Name: uvrB

Synonym: Kole_1730

Alternate gene names: 239618098

Gene position: 1859496-1857493 (Counterclockwise)

Preceding gene: 239618099

Following gene: 239618097

Centisome position: 80.77

GC content: 43.46

Gene sequence:

>2004_bases
ATGAGGTTCAACCTGAAAGCTCCCTTTGAACCAAGGGGGGATCAACCCGAGGCGATAACCAGGTTGGTGGAAGGTTTAAG
GCGAGGAGACAGGTTTCAAACTCTCCTTGGAGTCACGGGTTCAGGTAAAACCTTTACCATGGCCAGTATTATAGAACGGG
TTCAGCAACCGGCTTTGGTTATCTCCCCCAATAAGGCACTGGTAGCTCAGCTTTACAGGGAGTTCAGGAGCTTTTTCCCT
GAGAACAGGGTTGAACTCTTTATCAGTTACTATGACTACTATCAGCCAGAAGCCTATATTCCTACCAAAGACCTTTATAT
CGAAAAAGATGCAGATATAAATGATTTGTTGGCAAGAATGAGAATCTCCGCACTGAAATCCGTGCTGACACGCAAAGATG
TCGTTGTTGTTGCCAGTGTCTCCGCTATTTACGCCAGTGGAGACCCCAGGGATTTCCAGGAGCTGAACATATCACTGGAA
ATAGGTCAGAGGATTCCCAGAAATGAACTTGCCTTAAAACTAGCAAGTATACAGTACTCACGATCTGAAGACATCTCAAG
TGGAGGTGTTTTTCACCTCCGGGGTGATGTTGTTGAGATCTTCCCTCCTTATGAAGACTATGGTATAAGAATATATTTCT
TCGATGATGAAATCGAGCGAATTATTTCCTTCGATCCAATGAACAGGAAAACTCTCGAGGAGTTTGATAGGATTATCATA
TACCCTGCCAAAGAATTCGTCACCACCGAAGAAAAGATAAAGCATGCGGTCAAGGAGATCGAAAGAGATCTGGAATTACG
TGCGAAAGAACTGGAGAAGAACGGCAAATATCTTGAAGCACAGCGTTTAAAACAAAGAACCCTGTACGATCTTGAAATGC
TAACAACCCTCGGTTACTGCTCTGGCATCGAAAACTATTCGCGGTATTTCGATGGAAGAAAACCCGGTGAACCACCCTAC
ACAATTTTAGACTATTTTGATAAGAGCGAAATGATCGTATTCCTGGATGAATCACATATAACCGTTCCACAAATCAGAGC
TATGTATCATGGTGACCATTCTCGAAAGAAGAACCTTGTGGAATACGGATTCCGCCTTCCGTCAGCTTTTGATAACAGAC
CTCTCACATTTGAGGAGTTCTTAGAAAGTGTTGGACAGATAATTTTTGTGTCAGCCACTCCGGGAGATTACGAACTGAGT
GTTTCCACTCAGATTGTTGAGCAGCTCATAAGACCAACCGGTTTGATCGACCCGGAGGTGGTTGTCAAACCTACTCAAAA
TCAGGTAGATGATTTCATAGAAGAAGTACAAAAGGTAATAGAAAGAGGTGAACGGGCACTTGTTACGGTACTGACAAAAA
AGGCTGCCGAGATGTTCAGTGCTTACCTCAACGAACTCGGTATAAGAGCTGAATACCTGCATTCAGAACTCGATACGGTT
GAAAGGGTTGAGGTACTGAAAAAACTAAGGGAAGGCAGCGTTGATGTGGTTGTCGGCGTAAACCTGCTCAGGGAAGGGCT
CGACCTACCTGAGGTCTCCCTTGTTGCTATAATGGATGCGGATAAAGAAGGTTTTCTGCGCTCAGAAACGACCTTAATTC
AAACCATAGGAAGGGCAGCAAGAAATATCAACGGGAAGGTCCTGCTGTATGCTGACAGGATAACAAATTCCATGAAACGT
GCCATCGAAGAGACTAATCGGAGAAGAATGAAACAACTCATGTATAATATAGAACATGACATAAAACCTGAGAGTATCGT
TAAACCCCTGTACGAAAATATTTTTGAAGAGTTTGCTGACAACGAAGAAAAGATAGAAATCGCCAAAAACACCTACCTTG
ATGGCATTCTTGCGTTGAAAGAGGATCTTGAAGCTGAAGAATACCTTGCACTACTGGAAGAAGAGATGTGGAGGGCCTCA
AGTGAACTACGTTATGAGGATGCAGCGATGTTACGTGACGAGATGCTCAGAATCAAGCGAGAAACAAAGAAAGACAACAT
TTAA

Upstream 100 bases:

>100_bases
AAAGTTCTTGATCTCGCGATATCAGCCGCTCAAGAGATGTTCGCTGCGGATTTTAAGAAAGTCATGTCCCGCTACAACTC
TATAGGGGTGGAGTAGACTT

Downstream 100 bases:

>100_bases
GGGGCTGATCATATGAAAATCGTCGCTACAAACAAAAAAGCACGCTTTCAGTACCATATTCTCGAGACCTATGAAGCAGG
TATACAGCTACTGGGAACCG

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MRFNLKAPFEPRGDQPEAITRLVEGLRRGDRFQTLLGVTGSGKTFTMASIIERVQQPALVISPNKALVAQLYREFRSFFP
ENRVELFISYYDYYQPEAYIPTKDLYIEKDADINDLLARMRISALKSVLTRKDVVVVASVSAIYASGDPRDFQELNISLE
IGQRIPRNELALKLASIQYSRSEDISSGGVFHLRGDVVEIFPPYEDYGIRIYFFDDEIERIISFDPMNRKTLEEFDRIII
YPAKEFVTTEEKIKHAVKEIERDLELRAKELEKNGKYLEAQRLKQRTLYDLEMLTTLGYCSGIENYSRYFDGRKPGEPPY
TILDYFDKSEMIVFLDESHITVPQIRAMYHGDHSRKKNLVEYGFRLPSAFDNRPLTFEEFLESVGQIIFVSATPGDYELS
VSTQIVEQLIRPTGLIDPEVVVKPTQNQVDDFIEEVQKVIERGERALVTVLTKKAAEMFSAYLNELGIRAEYLHSELDTV
ERVEVLKKLREGSVDVVVGVNLLREGLDLPEVSLVAIMDADKEGFLRSETTLIQTIGRAARNINGKVLLYADRITNSMKR
AIEETNRRRMKQLMYNIEHDIKPESIVKPLYENIFEEFADNEEKIEIAKNTYLDGILALKEDLEAEEYLALLEEEMWRAS
SELRYEDAAMLRDEMLRIKRETKKDNI

Sequences:

>Translated_667_residues
MRFNLKAPFEPRGDQPEAITRLVEGLRRGDRFQTLLGVTGSGKTFTMASIIERVQQPALVISPNKALVAQLYREFRSFFP
ENRVELFISYYDYYQPEAYIPTKDLYIEKDADINDLLARMRISALKSVLTRKDVVVVASVSAIYASGDPRDFQELNISLE
IGQRIPRNELALKLASIQYSRSEDISSGGVFHLRGDVVEIFPPYEDYGIRIYFFDDEIERIISFDPMNRKTLEEFDRIII
YPAKEFVTTEEKIKHAVKEIERDLELRAKELEKNGKYLEAQRLKQRTLYDLEMLTTLGYCSGIENYSRYFDGRKPGEPPY
TILDYFDKSEMIVFLDESHITVPQIRAMYHGDHSRKKNLVEYGFRLPSAFDNRPLTFEEFLESVGQIIFVSATPGDYELS
VSTQIVEQLIRPTGLIDPEVVVKPTQNQVDDFIEEVQKVIERGERALVTVLTKKAAEMFSAYLNELGIRAEYLHSELDTV
ERVEVLKKLREGSVDVVVGVNLLREGLDLPEVSLVAIMDADKEGFLRSETTLIQTIGRAARNINGKVLLYADRITNSMKR
AIEETNRRRMKQLMYNIEHDIKPESIVKPLYENIFEEFADNEEKIEIAKNTYLDGILALKEDLEAEEYLALLEEEMWRAS
SELRYEDAAMLRDEMLRIKRETKKDNI
>Mature_667_residues
MRFNLKAPFEPRGDQPEAITRLVEGLRRGDRFQTLLGVTGSGKTFTMASIIERVQQPALVISPNKALVAQLYREFRSFFP
ENRVELFISYYDYYQPEAYIPTKDLYIEKDADINDLLARMRISALKSVLTRKDVVVVASVSAIYASGDPRDFQELNISLE
IGQRIPRNELALKLASIQYSRSEDISSGGVFHLRGDVVEIFPPYEDYGIRIYFFDDEIERIISFDPMNRKTLEEFDRIII
YPAKEFVTTEEKIKHAVKEIERDLELRAKELEKNGKYLEAQRLKQRTLYDLEMLTTLGYCSGIENYSRYFDGRKPGEPPY
TILDYFDKSEMIVFLDESHITVPQIRAMYHGDHSRKKNLVEYGFRLPSAFDNRPLTFEEFLESVGQIIFVSATPGDYELS
VSTQIVEQLIRPTGLIDPEVVVKPTQNQVDDFIEEVQKVIERGERALVTVLTKKAAEMFSAYLNELGIRAEYLHSELDTV
ERVEVLKKLREGSVDVVVGVNLLREGLDLPEVSLVAIMDADKEGFLRSETTLIQTIGRAARNINGKVLLYADRITNSMKR
AIEETNRRRMKQLMYNIEHDIKPESIVKPLYENIFEEFADNEEKIEIAKNTYLDGILALKEDLEAEEYLALLEEEMWRAS
SELRYEDAAMLRDEMLRIKRETKKDNI

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=669, Percent_Identity=52.1674140508221, Blast_Score=658, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=224, Percent_Identity=26.3392857142857, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_KOSOT (C5CFR8)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941420.1
- GeneID:   7968131
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1730
- ProtClustDB:   PRK05298
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 77157; Mature: 77157

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNLKAPFEPRGDQPEAITRLVEGLRRGDRFQTLLGVTGSGKTFTMASIIERVQQPALV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCCEEHHHHHHHHHCCCCEE
ISPNKALVAQLYREFRSFFPENRVELFISYYDYYQPEAYIPTKDLYIEKDADINDLLARM
ECCCHHHHHHHHHHHHHHCCCCHHEEEEEHHHCCCCCCCCCCCCEEEECCCCHHHHHHHH
RISALKSVLTRKDVVVVASVSAIYASGDPRDFQELNISLEIGQRIPRNELALKLASIQYS
HHHHHHHHHHHCCEEEEEEEHHHEECCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCC
RSEDISSGGVFHLRGDVVEIFPPYEDYGIRIYFFDDEIERIISFDPMNRKTLEEFDRIII
CCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHCCEEE
YPAKEFVTTEEKIKHAVKEIERDLELRAKELEKNGKYLEAQRLKQRTLYDLEMLTTLGYC
EECHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SGIENYSRYFDGRKPGEPPYTILDYFDKSEMIVFLDESHITVPQIRAMYHGDHSRKKNLV
HCHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHH
EYGFRLPSAFDNRPLTFEEFLESVGQIIFVSATPGDYELSVSTQIVEQLIRPTGLIDPEV
HHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHCCCCCCCCCE
VVKPTQNQVDDFIEEVQKVIERGERALVTVLTKKAAEMFSAYLNELGIRAEYLHSELDTV
EEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ERVEVLKKLREGSVDVVVGVNLLREGLDLPEVSLVAIMDADKEGFLRSETTLIQTIGRAA
HHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
RNINGKVLLYADRITNSMKRAIEETNRRRMKQLMYNIEHDIKPESIVKPLYENIFEEFAD
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
NEEKIEIAKNTYLDGILALKEDLEAEEYLALLEEEMWRASSELRYEDAAMLRDEMLRIKR
CCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ETKKDNI
HHCCCCC
>Mature Secondary Structure
MRFNLKAPFEPRGDQPEAITRLVEGLRRGDRFQTLLGVTGSGKTFTMASIIERVQQPALV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCCEEHHHHHHHHHCCCCEE
ISPNKALVAQLYREFRSFFPENRVELFISYYDYYQPEAYIPTKDLYIEKDADINDLLARM
ECCCHHHHHHHHHHHHHHCCCCHHEEEEEHHHCCCCCCCCCCCCEEEECCCCHHHHHHHH
RISALKSVLTRKDVVVVASVSAIYASGDPRDFQELNISLEIGQRIPRNELALKLASIQYS
HHHHHHHHHHHCCEEEEEEEHHHEECCCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCC
RSEDISSGGVFHLRGDVVEIFPPYEDYGIRIYFFDDEIERIISFDPMNRKTLEEFDRIII
CCCCCCCCCEEEEECCEEEECCCCCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHCCEEE
YPAKEFVTTEEKIKHAVKEIERDLELRAKELEKNGKYLEAQRLKQRTLYDLEMLTTLGYC
EECHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SGIENYSRYFDGRKPGEPPYTILDYFDKSEMIVFLDESHITVPQIRAMYHGDHSRKKNLV
HCHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHH
EYGFRLPSAFDNRPLTFEEFLESVGQIIFVSATPGDYELSVSTQIVEQLIRPTGLIDPEV
HHHCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHCCCCCCCCCE
VVKPTQNQVDDFIEEVQKVIERGERALVTVLTKKAAEMFSAYLNELGIRAEYLHSELDTV
EEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ERVEVLKKLREGSVDVVVGVNLLREGLDLPEVSLVAIMDADKEGFLRSETTLIQTIGRAA
HHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
RNINGKVLLYADRITNSMKRAIEETNRRRMKQLMYNIEHDIKPESIVKPLYENIFEEFAD
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
NEEKIEIAKNTYLDGILALKEDLEAEEYLALLEEEMWRASSELRYEDAAMLRDEMLRIKR
CCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ETKKDNI
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA