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The map label for this gene is smpB [H]

Identifier: 239618097

GI number: 239618097

Start: 1857037

End: 1857480

Strand: Reverse

Name: smpB [H]

Synonym: Kole_1729

Alternate gene names: 239618097

Gene position: 1857480-1857037 (Counterclockwise)

Preceding gene: 239618098

Following gene: 239618096

Centisome position: 80.69

GC content: 41.67

Gene sequence:

>444_bases
ATGAAAATCGTCGCTACAAACAAAAAAGCACGCTTTCAGTACCATATTCTCGAGACCTATGAAGCAGGTATACAGCTACT
GGGAACCGAGGTTAAATCCTTAAGAAATGGTTCAGCTTCCATATCGGAAGCTTTCTGCAAGTTTGAGGACGGAGAGCTCT
TTCTAATAAACGCAAACATTAGCCAGTATAGTCATGGAACACACTGGAACCACGATCCTTTAAGGAAAAGGAAACTTCTT
ATGCATAAAAGGGAGTTGCTTCGAATCGCTCAAAAAGTCAAAGAACGTGGATTGACACTGATACCTACAAAAATATACTT
CAACGACAAAGGTATCGCGAAAGTGGAGATTGCCCTGGCAAAAGGAAAACGGCTTTACGACAAACGTGAAGATATAGCGA
AGCGTGATCTGGAAAGGCGTCTTAAAAGGCGTGTTGACTATTGA

Upstream 100 bases:

>100_bases
CTCAAGTGAACTACGTTATGAGGATGCAGCGATGTTACGTGACGAGATGCTCAGAATCAAGCGAGAAACAAAGAAAGACA
ACATTTAAGGGGCTGATCAT

Downstream 100 bases:

>100_bases
AAACAACACCCATCGAACGATTTTAGGTATCAACCTGTCGAAAATATGAGAACAGTGGTTATGATCAATAGACTGGGGGT
GTTTTTGTGAGGAAAACTAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MKIVATNKKARFQYHILETYEAGIQLLGTEVKSLRNGSASISEAFCKFEDGELFLINANISQYSHGTHWNHDPLRKRKLL
MHKRELLRIAQKVKERGLTLIPTKIYFNDKGIAKVEIALAKGKRLYDKREDIAKRDLERRLKRRVDY

Sequences:

>Translated_147_residues
MKIVATNKKARFQYHILETYEAGIQLLGTEVKSLRNGSASISEAFCKFEDGELFLINANISQYSHGTHWNHDPLRKRKLL
MHKRELLRIAQKVKERGLTLIPTKIYFNDKGIAKVEIALAKGKRLYDKREDIAKRDLERRLKRRVDY
>Mature_147_residues
MKIVATNKKARFQYHILETYEAGIQLLGTEVKSLRNGSASISEAFCKFEDGELFLINANISQYSHGTHWNHDPLRKRKLL
MHKRELLRIAQKVKERGLTLIPTKIYFNDKGIAKVEIALAKGKRLYDKREDIAKRDLERRLKRRVDY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=136, Percent_Identity=44.1176470588235, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17226; Mature: 17226

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVATNKKARFQYHILETYEAGIQLLGTEVKSLRNGSASISEAFCKFEDGELFLINANI
CEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCH
SQYSHGTHWNHDPLRKRKLLMHKRELLRIAQKVKERGLTLIPTKIYFNDKGIAKVEIALA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEC
KGKRLYDKREDIAKRDLERRLKRRVDY
CCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIVATNKKARFQYHILETYEAGIQLLGTEVKSLRNGSASISEAFCKFEDGELFLINANI
CEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCH
SQYSHGTHWNHDPLRKRKLLMHKRELLRIAQKVKERGLTLIPTKIYFNDKGIAKVEIALA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEC
KGKRLYDKREDIAKRDLERRLKRRVDY
CCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]