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The map label for this gene is zraS [H]

Identifier: 239617965

GI number: 239617965

Start: 1709359

End: 1710645

Strand: Reverse

Name: zraS [H]

Synonym: Kole_1593

Alternate gene names: 239617965

Gene position: 1710645-1709359 (Counterclockwise)

Preceding gene: 239617966

Following gene: 239617964

Centisome position: 74.31

GC content: 42.42

Gene sequence:

>1287_bases
TTGTTCCTAACAAGATGGAATAAAGAGGATTTCAGAATACCGTTGACCGTTGCTATCTGGACTTTTTTGAGCGGTTTTGT
TGTGTTGTTCTTGTATGGTTCGCTATCCGTAAGTAGTTTTGTAGCGCTTATGGTTGCAGCAGGTGCAGGTTTTCTGGTTT
CCCATTACCTTTTTAACATCCCTCTTTTTAAGCTTTTGAACGCTTTCGCCGGGAGAAGGGAGGTTCCCCCGGGGAAACTG
CATGGCATCTACCGCCAGATCTACGAAGCTTATAAGAATGTTGTATATCAGTTAGGACAGGAATGGGAGCTTTACAGAAA
GCTGTATTCAGATTTTGCCGAGGTTCTGGATTCTCTCAACATCGCTATTGTCATAATCGATGATTCCTTTCAAATCAAGA
TAGCAAATAAGGCCTTCAAGGAATACTTCCTTGAGAGGCACAATACCAAAGCAAAGGATTTTAGAGCTTTAATGAAGCGT
AGTGGTTTCCGCTTTTCGTTTGAAGATGGAAACTATGAGATTTACTCCCGCCGTTTGAAGAAGAGATTCCTTGTCTCCAT
CGTCAGGAAGAACAAAGGGTGGATCGTCAGTTTCTCGGATGTAACTGCCTACTGGAAAACAAAACGGCTTCTGGAAAAAA
CCAGAAAATACGCAGTCAGCGCTGAAAGCGTCGCAGATCTTGCACACGGTCTCAAACAACCTCTTGCAACCATACAATTA
ACCTTTGATATGTATATTCGCACGAAGGATGAGAAATATCTGGAGCAACTCAAGAGAAACCTGTCAGAGTTTCGCGAAAA
AATCGCTGCGGTTCTGCAGTTGTACCATTTCGGAGAAGATTTTGGAGAAGTAAACCTTAAAGATTTGCTCGAAAGAATTA
TGGGATATATGAGTTCCATTGCTGAATCCAGGAAGGTGCGTCTCTCCTGTCGCTATCACGGCGATGGGAAGATTCAGGTT
CAGTCAAACAGGCTTGAGAATGTTTTGAAAAACCTGATCTTGAATGCTATAGAAGCCTGCGAAAATGTTGACGACGCGTC
GGTTACCGTTAAACTTCGTGAAGGAAGCGATATCGTGACAGTCATTATCTGTGACAATGGTCCTGGAATAGAACCAAAAA
ACGCTGAGAAAATATTCAAGATCTTCTTCACCACAAAACCACAGGGAACAGGGTTTGGATTAGCTTTAGCCAAAAGCTTT
TGTGAGGACAATGGTGCTATATTAAGATTTAAGAGTATTCCTGGAAAGGGTACCGTTTTTTCGCTAACGATGAAGAAGGT
GGAGTGA

Upstream 100 bases:

>100_bases
ATTCTCGTCCACATTGATAGCAGTGGCTTTATAGAAATCGCTGTGAATGAGGGAAGTGCAAAATCTCTTATCAAAGCGAG
AGAAGGTGAGTTGATAGAAA

Downstream 100 bases:

>100_bases
AATGAAAAGAAAATTGCTGGTTGTGGACGATGATATGGCATTCAATTTAATACTTTGCGAAGCACTGGAAAGCGAAGGAT
ATCTCGTAACAAAAGCCCAC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MFLTRWNKEDFRIPLTVAIWTFLSGFVVLFLYGSLSVSSFVALMVAAGAGFLVSHYLFNIPLFKLLNAFAGRREVPPGKL
HGIYRQIYEAYKNVVYQLGQEWELYRKLYSDFAEVLDSLNIAIVIIDDSFQIKIANKAFKEYFLERHNTKAKDFRALMKR
SGFRFSFEDGNYEIYSRRLKKRFLVSIVRKNKGWIVSFSDVTAYWKTKRLLEKTRKYAVSAESVADLAHGLKQPLATIQL
TFDMYIRTKDEKYLEQLKRNLSEFREKIAAVLQLYHFGEDFGEVNLKDLLERIMGYMSSIAESRKVRLSCRYHGDGKIQV
QSNRLENVLKNLILNAIEACENVDDASVTVKLREGSDIVTVIICDNGPGIEPKNAEKIFKIFFTTKPQGTGFGLALAKSF
CEDNGAILRFKSIPGKGTVFSLTMKKVE

Sequences:

>Translated_428_residues
MFLTRWNKEDFRIPLTVAIWTFLSGFVVLFLYGSLSVSSFVALMVAAGAGFLVSHYLFNIPLFKLLNAFAGRREVPPGKL
HGIYRQIYEAYKNVVYQLGQEWELYRKLYSDFAEVLDSLNIAIVIIDDSFQIKIANKAFKEYFLERHNTKAKDFRALMKR
SGFRFSFEDGNYEIYSRRLKKRFLVSIVRKNKGWIVSFSDVTAYWKTKRLLEKTRKYAVSAESVADLAHGLKQPLATIQL
TFDMYIRTKDEKYLEQLKRNLSEFREKIAAVLQLYHFGEDFGEVNLKDLLERIMGYMSSIAESRKVRLSCRYHGDGKIQV
QSNRLENVLKNLILNAIEACENVDDASVTVKLREGSDIVTVIICDNGPGIEPKNAEKIFKIFFTTKPQGTGFGLALAKSF
CEDNGAILRFKSIPGKGTVFSLTMKKVE
>Mature_428_residues
MFLTRWNKEDFRIPLTVAIWTFLSGFVVLFLYGSLSVSSFVALMVAAGAGFLVSHYLFNIPLFKLLNAFAGRREVPPGKL
HGIYRQIYEAYKNVVYQLGQEWELYRKLYSDFAEVLDSLNIAIVIIDDSFQIKIANKAFKEYFLERHNTKAKDFRALMKR
SGFRFSFEDGNYEIYSRRLKKRFLVSIVRKNKGWIVSFSDVTAYWKTKRLLEKTRKYAVSAESVADLAHGLKQPLATIQL
TFDMYIRTKDEKYLEQLKRNLSEFREKIAAVLQLYHFGEDFGEVNLKDLLERIMGYMSSIAESRKVRLSCRYHGDGKIQV
QSNRLENVLKNLILNAIEACENVDDASVTVKLREGSDIVTVIICDNGPGIEPKNAEKIFKIFFTTKPQGTGFGLALAKSF
CEDNGAILRFKSIPGKGTVFSLTMKKVE

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=29.1666666666667, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49135; Mature: 49135

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLTRWNKEDFRIPLTVAIWTFLSGFVVLFLYGSLSVSSFVALMVAAGAGFLVSHYLFNI
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
PLFKLLNAFAGRREVPPGKLHGIYRQIYEAYKNVVYQLGQEWELYRKLYSDFAEVLDSLN
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
IAIVIIDDSFQIKIANKAFKEYFLERHNTKAKDFRALMKRSGFRFSFEDGNYEIYSRRLK
EEEEEEECCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
KRFLVSIVRKNKGWIVSFSDVTAYWKTKRLLEKTRKYAVSAESVADLAHGLKQPLATIQL
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEEEE
TFDMYIRTKDEKYLEQLKRNLSEFREKIAAVLQLYHFGEDFGEVNLKDLLERIMGYMSSI
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AESRKVRLSCRYHGDGKIQVQSNRLENVLKNLILNAIEACENVDDASVTVKLREGSDIVT
HHCCEEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
VIICDNGPGIEPKNAEKIFKIFFTTKPQGTGFGLALAKSFCEDNGAILRFKSIPGKGTVF
EEEECCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEE
SLTMKKVE
EEEEECCC
>Mature Secondary Structure
MFLTRWNKEDFRIPLTVAIWTFLSGFVVLFLYGSLSVSSFVALMVAAGAGFLVSHYLFNI
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
PLFKLLNAFAGRREVPPGKLHGIYRQIYEAYKNVVYQLGQEWELYRKLYSDFAEVLDSLN
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
IAIVIIDDSFQIKIANKAFKEYFLERHNTKAKDFRALMKRSGFRFSFEDGNYEIYSRRLK
EEEEEEECCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
KRFLVSIVRKNKGWIVSFSDVTAYWKTKRLLEKTRKYAVSAESVADLAHGLKQPLATIQL
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEEEE
TFDMYIRTKDEKYLEQLKRNLSEFREKIAAVLQLYHFGEDFGEVNLKDLLERIMGYMSSI
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AESRKVRLSCRYHGDGKIQVQSNRLENVLKNLILNAIEACENVDDASVTVKLREGSDIVT
HHCCEEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
VIICDNGPGIEPKNAEKIFKIFFTTKPQGTGFGLALAKSFCEDNGAILRFKSIPGKGTVF
EEEECCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEE
SLTMKKVE
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]