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The map label for this gene is ispE [H]

Identifier: 239617961

GI number: 239617961

Start: 1703990

End: 1704862

Strand: Reverse

Name: ispE [H]

Synonym: Kole_1589

Alternate gene names: 239617961

Gene position: 1704862-1703990 (Counterclockwise)

Preceding gene: 239617962

Following gene: 239617960

Centisome position: 74.06

GC content: 41.35

Gene sequence:

>873_bases
TTGGTCGAAACTGGTGTTGAAAGAAATAACGGGTTGTTAAAAGCTTACGCCAAATTAAATCTTTACCTCGCTGTCGGGCC
GAAGCGTGATGACAGTTTTCACGAGCTTCTCACGCTTTTTCAAACGATATCACTTTACGACGAACTGTTCGTTGAAGATA
GGCCCATACCTGGGGTATTGTTTGACAGTGATGCTAATTTGAAATGGAGTCCTGAAAACACCATATACAGAGCCTGTGAA
CTCTTTGAACAACTAACAGGAAACAGGCTTTACTTGCGTATATTTCTAAGAAAACGAATACCATCAGGCGGAGGGCTGGG
TGGCGGAAGTTCTGACGCAGCCACGATTTTAAGCTTTCTAGCCTCGAAATACAGGGTCGATGCCAAAGCGATCTACGAGA
TCTCCAAAAAAATTGGCAGTGACGTTCCTTTCTTTTTGAAAGGAGGGACCGCCATAGGAAAAGGGCGCGGTGAAATTCTA
GAATATCTGAAAGGCTTAAGTGAATACCGGGTCTCGCTGATTTTTCCAAACGTTTCAGTTCAAACAGCGTATGCGTACAA
ACTCTTAGACGAAAGAGGAACGAAAAAAATTCTGGATGATGGCAATGTTTATAAACTTTATGAAGCTTTCTTAGAGAAAA
ATTACAATAGAATCAGAGAATTATCCAAAAATGACTTTGAAAAAGTCGTTTCTGAAGCTTTTCCGGAGATTAAAGGAAAA
TACGTGGAGGCAATCGAATCCCTGAAAGAGGATGCAATAATGGTAAGGATGTCTGGTTCTGGAAGCACGTTATTCATGCT
GTACCCTCCTGAAAGTAAAAATGGGGAATATTCTTTTCTTCCAGCGATTGGGGGAAAAAACGATGACCTTTGA

Upstream 100 bases:

>100_bases
TAGCAGAGGTAGAGTTTTTCGGTCTCAGAGAAGAGGATATGTTTGAAATCCAATGGATTCATCCTGATGAACTCTGTAAA
AAAGATTGGGGACTTGAAGG

Downstream 100 bases:

>100_bases
TGGTGTTATCCTCTACAAAGTTTCAAAGACCATGACAAAAATCCAGAACGAGTCTCTGAGGCAGGTTTATCAAAAGGATA
GATGGAACTTCTTCTTCAAG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MVETGVERNNGLLKAYAKLNLYLAVGPKRDDSFHELLTLFQTISLYDELFVEDRPIPGVLFDSDANLKWSPENTIYRACE
LFEQLTGNRLYLRIFLRKRIPSGGGLGGGSSDAATILSFLASKYRVDAKAIYEISKKIGSDVPFFLKGGTAIGKGRGEIL
EYLKGLSEYRVSLIFPNVSVQTAYAYKLLDERGTKKILDDGNVYKLYEAFLEKNYNRIRELSKNDFEKVVSEAFPEIKGK
YVEAIESLKEDAIMVRMSGSGSTLFMLYPPESKNGEYSFLPAIGGKNDDL

Sequences:

>Translated_290_residues
MVETGVERNNGLLKAYAKLNLYLAVGPKRDDSFHELLTLFQTISLYDELFVEDRPIPGVLFDSDANLKWSPENTIYRACE
LFEQLTGNRLYLRIFLRKRIPSGGGLGGGSSDAATILSFLASKYRVDAKAIYEISKKIGSDVPFFLKGGTAIGKGRGEIL
EYLKGLSEYRVSLIFPNVSVQTAYAYKLLDERGTKKILDDGNVYKLYEAFLEKNYNRIRELSKNDFEKVVSEAFPEIKGK
YVEAIESLKEDAIMVRMSGSGSTLFMLYPPESKNGEYSFLPAIGGKNDDL
>Mature_290_residues
MVETGVERNNGLLKAYAKLNLYLAVGPKRDDSFHELLTLFQTISLYDELFVEDRPIPGVLFDSDANLKWSPENTIYRACE
LFEQLTGNRLYLRIFLRKRIPSGGGLGGGSSDAATILSFLASKYRVDAKAIYEISKKIGSDVPFFLKGGTAIGKGRGEIL
EYLKGLSEYRVSLIFPNVSVQTAYAYKLLDERGTKKILDDGNVYKLYEAFLEKNYNRIRELSKNDFEKVVSEAFPEIKGK
YVEAIESLKEDAIMVRMSGSGSTLFMLYPPESKNGEYSFLPAIGGKNDDL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=275, Percent_Identity=29.4545454545455, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 32534; Mature: 32534

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVETGVERNNGLLKAYAKLNLYLAVGPKRDDSFHELLTLFQTISLYDELFVEDRPIPGVL
CCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FDSDANLKWSPENTIYRACELFEQLTGNRLYLRIFLRKRIPSGGGLGGGSSDAATILSFL
ECCCCCCEECCCHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHH
ASKYRVDAKAIYEISKKIGSDVPFFLKGGTAIGKGRGEILEYLKGLSEYRVSLIFPNVSV
HHHHCCCHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHHCEEEEEECCCCC
QTAYAYKLLDERGTKKILDDGNVYKLYEAFLEKNYNRIRELSKNDFEKVVSEAFPEIKGK
HHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHHH
YVEAIESLKEDAIMVRMSGSGSTLFMLYPPESKNGEYSFLPAIGGKNDDL
HHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MVETGVERNNGLLKAYAKLNLYLAVGPKRDDSFHELLTLFQTISLYDELFVEDRPIPGVL
CCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FDSDANLKWSPENTIYRACELFEQLTGNRLYLRIFLRKRIPSGGGLGGGSSDAATILSFL
ECCCCCCEECCCHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHH
ASKYRVDAKAIYEISKKIGSDVPFFLKGGTAIGKGRGEILEYLKGLSEYRVSLIFPNVSV
HHHHCCCHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHHCEEEEEECCCCC
QTAYAYKLLDERGTKKILDDGNVYKLYEAFLEKNYNRIRELSKNDFEKVVSEAFPEIKGK
HHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHHH
YVEAIESLKEDAIMVRMSGSGSTLFMLYPPESKNGEYSFLPAIGGKNDDL
HHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]