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The map label for this gene is dacB [H]

Identifier: 239617263

GI number: 239617263

Start: 917832

End: 918974

Strand: Reverse

Name: dacB [H]

Synonym: Kole_0872

Alternate gene names: 239617263

Gene position: 918974-917832 (Counterclockwise)

Preceding gene: 239617264

Following gene: 239617261

Centisome position: 39.92

GC content: 39.11

Gene sequence:

>1143_bases
ATGAAAAAAGTAATTTTCTTAATTTTCATCCTTTTAACTACCTCAATATTTGGGGATACGGTGAGAGAACTTGTGGATAA
CTTTGATGGATTCGTTGGGATCTATGTTGAAGATATCGAAACAGGGGAAATTATTACCCAGTATAACGCTACAAAGCTGT
TTACGCCAGCGTCCGTCACAAAGTTATTTACACTGTTAGCTGCCTTAGAAAACCTTGGTTTGGAATACACCTACAAAACG
AAATTTTATTTGATCGGAGAAGATTTCGGGGTAAAAGGTTCCGGAGATCCAACTATTTACGTATCCACACTTCACGACAT
CATAGCCAAAATAGTGGAAGATAAGAAGATCGTTGAGATAAATAACATAATTCTTGACGACAGACTTTTCGATAGAAAAG
AGATCTATGGAAGAGGTTGGATGTGGGATGATCCCAATCCGATGATAGGCGCTCTTACGATAAAAGGGCCTTCGTATCCA
TCGATCTCTTCTGGAATTGATCAGTATACACTAACCAGAAAATTTTTTGAGACATTGAAAAGCACCTTTGAAGAACTGGG
TGTTTCCGTAAAAGGTGAAATGGTTGTCAAATACATATCAAAAAAACACGAACCGGCTTATATCCACGAATCCAAACCAT
TGAGAGAGATTCTGAAAAGCATGATCGAGGTAAGCGACAATCAGGTAGCCGAACAGATTTTCAGGACAGTGGGAGCAAAG
GTATTGAAAAAAGGAACGATCAACAACTCCATAGCAGCCCTGATAAGAACCGTTAAAAGTGTCACAGAGTACGGCGTGAA
CGATTTTACGATCACCGATGGTCCTGGTCTTTCAAGATACAACCTTGTTTCCCCAAAACTGGTTGTGGAAATCCTGAAGC
ACATGTACAAAAAGTATGGAGATACACTTCTCGACATTCTTTCATATTCGAAAAATGATGGAACAATAAAAGGGAGATTC
GATTTTGAAGTCTGGGGAAAGACGGGAACGCTTACGGGAGTTTCCGGATTGGCAGGTTTTATGAAAACAAAATCGGGGAA
AATGGTGGTATTTTCACTGTTCGAAAACAACTTTTCAAAGTCAAAAACCGATGCCAAAGGTTTTGAAAACAGTATTCTGG
AAATATTTTATGAGGGGAACTGA

Upstream 100 bases:

>100_bases
TGATGGCTGGTTCGAGTATTGCGACTATTCCAGTAATTGTGGTTTTTCTGGCCTTTCAGAAACAAATCATATCCGGTCTG
GTCAGAGGGGCTGTTAAAGA

Downstream 100 bases:

>100_bases
AAACTGAGAACCGAGAGTCCGAGATAGCAGCTACGCTGCTGAAGTGCGGAGCTACGCTCCGGACGCTCACGAGGTTGAGG
TTTGCAGACGCTGTGCGCTG

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKKVIFLIFILLTTSIFGDTVRELVDNFDGFVGIYVEDIETGEIITQYNATKLFTPASVTKLFTLLAALENLGLEYTYKT
KFYLIGEDFGVKGSGDPTIYVSTLHDIIAKIVEDKKIVEINNIILDDRLFDRKEIYGRGWMWDDPNPMIGALTIKGPSYP
SISSGIDQYTLTRKFFETLKSTFEELGVSVKGEMVVKYISKKHEPAYIHESKPLREILKSMIEVSDNQVAEQIFRTVGAK
VLKKGTINNSIAALIRTVKSVTEYGVNDFTITDGPGLSRYNLVSPKLVVEILKHMYKKYGDTLLDILSYSKNDGTIKGRF
DFEVWGKTGTLTGVSGLAGFMKTKSGKMVVFSLFENNFSKSKTDAKGFENSILEIFYEGN

Sequences:

>Translated_380_residues
MKKVIFLIFILLTTSIFGDTVRELVDNFDGFVGIYVEDIETGEIITQYNATKLFTPASVTKLFTLLAALENLGLEYTYKT
KFYLIGEDFGVKGSGDPTIYVSTLHDIIAKIVEDKKIVEINNIILDDRLFDRKEIYGRGWMWDDPNPMIGALTIKGPSYP
SISSGIDQYTLTRKFFETLKSTFEELGVSVKGEMVVKYISKKHEPAYIHESKPLREILKSMIEVSDNQVAEQIFRTVGAK
VLKKGTINNSIAALIRTVKSVTEYGVNDFTITDGPGLSRYNLVSPKLVVEILKHMYKKYGDTLLDILSYSKNDGTIKGRF
DFEVWGKTGTLTGVSGLAGFMKTKSGKMVVFSLFENNFSKSKTDAKGFENSILEIFYEGN
>Mature_380_residues
MKKVIFLIFILLTTSIFGDTVRELVDNFDGFVGIYVEDIETGEIITQYNATKLFTPASVTKLFTLLAALENLGLEYTYKT
KFYLIGEDFGVKGSGDPTIYVSTLHDIIAKIVEDKKIVEINNIILDDRLFDRKEIYGRGWMWDDPNPMIGALTIKGPSYP
SISSGIDQYTLTRKFFETLKSTFEELGVSVKGEMVVKYISKKHEPAYIHESKPLREILKSMIEVSDNQVAEQIFRTVGAK
VLKKGTINNSIAALIRTVKSVTEYGVNDFTITDGPGLSRYNLVSPKLVVEILKHMYKKYGDTLLDILSYSKNDGTIKGRF
DFEVWGKTGTLTGVSGLAGFMKTKSGKMVVFSLFENNFSKSKTDAKGFENSILEIFYEGN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=221, Percent_Identity=26.6968325791855, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42682; Mature: 42682

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIFLIFILLTTSIFGDTVRELVDNFDGFVGIYVEDIETGEIITQYNATKLFTPASVT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCEEECCHHHH
KLFTLLAALENLGLEYTYKTKFYLIGEDFGVKGSGDPTIYVSTLHDIIAKIVEDKKIVEI
HHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEE
NNIILDDRLFDRKEIYGRGWMWDDPNPMIGALTIKGPSYPSISSGIDQYTLTRKFFETLK
CCEEECCCHHCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHH
STFEELGVSVKGEMVVKYISKKHEPAYIHESKPLREILKSMIEVSDNQVAEQIFRTVGAK
HHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VLKKGTINNSIAALIRTVKSVTEYGVNDFTITDGPGLSRYNLVSPKLVVEILKHMYKKYG
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
DTLLDILSYSKNDGTIKGRFDFEVWGKTGTLTGVSGLAGFMKTKSGKMVVFSLFENNFSK
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEHHCCCCC
SKTDAKGFENSILEIFYEGN
CCCHHHHHHCCEEEEEECCC
>Mature Secondary Structure
MKKVIFLIFILLTTSIFGDTVRELVDNFDGFVGIYVEDIETGEIITQYNATKLFTPASVT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCEEECCHHHH
KLFTLLAALENLGLEYTYKTKFYLIGEDFGVKGSGDPTIYVSTLHDIIAKIVEDKKIVEI
HHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEEEE
NNIILDDRLFDRKEIYGRGWMWDDPNPMIGALTIKGPSYPSISSGIDQYTLTRKFFETLK
CCEEECCCHHCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHH
STFEELGVSVKGEMVVKYISKKHEPAYIHESKPLREILKSMIEVSDNQVAEQIFRTVGAK
HHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VLKKGTINNSIAALIRTVKSVTEYGVNDFTITDGPGLSRYNLVSPKLVVEILKHMYKKYG
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
DTLLDILSYSKNDGTIKGRFDFEVWGKTGTLTGVSGLAGFMKTKSGKMVVFSLFENNFSK
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEHHCCCCC
SKTDAKGFENSILEIFYEGN
CCCHHHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]