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The map label for this gene is cobB [H]

Identifier: 239617254

GI number: 239617254

Start: 908507

End: 909256

Strand: Reverse

Name: cobB [H]

Synonym: Kole_0862

Alternate gene names: 239617254

Gene position: 909256-908507 (Counterclockwise)

Preceding gene: 239617255

Following gene: 239617248

Centisome position: 39.5

GC content: 42.67

Gene sequence:

>750_bases
ATGAATCTGGAAGAATTGGCGGAAGAGTTCCTCGAATTTTTGGATGAATCCGAATCTACGGCGATTCTTACCGGTGCCGG
AGTTTCGACTGCAAGCGGTATTCCGGATTTCAGAGGGCCACAAGGTTTGTATAAGAAACTCCCTCAATACATTTTTGATC
TCGATTTTTTCCTGTCACAACCGGCTGAGTACTACAAGATTGCTGCAGATAGAATTCACAACCTTTTTAACAAAGAACCT
AATGCCACTCACAGGTTACTGGCAATGCTGGAGAAAAAAGGAATGATAGAAGGAGTAATTACCCAGAATATCGATGGTTT
GCATCAGAAAGCTGGATCAAAGAAAGTGATTGAATTGCATGGCAACGCTCAGAAATTCTTCTGTATGAGCTGTGGGAAGC
GCTACACGGCGGAAGACGTTTTAAAAATGCTTGAAGTAAGTGATGTTCCTAAATGTACCTGTGGTGGACTCATAAAACCG
GATGTTGTTTTCTTTGGAGAAGCTCTTCCAGAAAGTGCCATGGCTGAAGCTTACATCCTTTCGGAAAATGCTGAACTCTT
TATTACCATGGGGTCTTCCCTTGTGGTGTATCCGGCGGCCCATTTACCTACCTTTGCAAAACAAAAGGGAGCAAAATTGC
TCATAATCAATCAGGGTGAAACAGGGCTTGATTATATAGCAGACAAGAAGTACGAGTGCGATTTAGCAGAGTTTTCAGAA
AGGATTATTTCCCTTCTGGGCGAAGTATAG

Upstream 100 bases:

>100_bases
TTCTAAAATTCTCGAAAACAATGTTCTTTTGTGGACTGGAGATGGGGTGATAAGATTCCCGGAGGTCATTTTTGAACCTC
TTGATTGAGAGACGAGGGGT

Downstream 100 bases:

>100_bases
CGAACCGGATTTTACCGCTGAGATCTCTTCTGAATTTGACTGTGTAACGTTTCCGGTATTTCTTAAGAAAGTCTTTCAAC
GGATTCCGGGAGTAAGTGGC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog; Sir2Tm [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNLEELAEEFLEFLDESESTAILTGAGVSTASGIPDFRGPQGLYKKLPQYIFDLDFFLSQPAEYYKIAADRIHNLFNKEP
NATHRLLAMLEKKGMIEGVITQNIDGLHQKAGSKKVIELHGNAQKFFCMSCGKRYTAEDVLKMLEVSDVPKCTCGGLIKP
DVVFFGEALPESAMAEAYILSENAELFITMGSSLVVYPAAHLPTFAKQKGAKLLIINQGETGLDYIADKKYECDLAEFSE
RIISLLGEV

Sequences:

>Translated_249_residues
MNLEELAEEFLEFLDESESTAILTGAGVSTASGIPDFRGPQGLYKKLPQYIFDLDFFLSQPAEYYKIAADRIHNLFNKEP
NATHRLLAMLEKKGMIEGVITQNIDGLHQKAGSKKVIELHGNAQKFFCMSCGKRYTAEDVLKMLEVSDVPKCTCGGLIKP
DVVFFGEALPESAMAEAYILSENAELFITMGSSLVVYPAAHLPTFAKQKGAKLLIINQGETGLDYIADKKYECDLAEFSE
RIISLLGEV
>Mature_249_residues
MNLEELAEEFLEFLDESESTAILTGAGVSTASGIPDFRGPQGLYKKLPQYIFDLDFFLSQPAEYYKIAADRIHNLFNKEP
NATHRLLAMLEKKGMIEGVITQNIDGLHQKAGSKKVIELHGNAQKFFCMSCGKRYTAEDVLKMLEVSDVPKCTCGGLIKP
DVVFFGEALPESAMAEAYILSENAELFITMGSSLVVYPAAHLPTFAKQKGAKLLIINQGETGLDYIADKKYECDLAEFSE
RIISLLGEV

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form (Probable). Also able to ADP- ribosylate peptide substrates with Arg or Lys in the +2 position. The role of this function in vivo is not clear [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912660, Length=264, Percent_Identity=34.8484848484849, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI63054862, Length=242, Percent_Identity=36.3636363636364, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI300797577, Length=260, Percent_Identity=31.5384615384615, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI7657575, Length=243, Percent_Identity=32.0987654320988, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI300795542, Length=213, Percent_Identity=37.5586854460094, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI13787215, Length=229, Percent_Identity=35.3711790393013, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI6912664, Length=229, Percent_Identity=35.3711790393013, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI6912662, Length=255, Percent_Identity=32.156862745098, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI300797597, Length=251, Percent_Identity=30.6772908366534, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI13775602, Length=216, Percent_Identity=35.1851851851852, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI13775600, Length=209, Percent_Identity=35.4066985645933, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI300797705, Length=188, Percent_Identity=35.1063829787234, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI215982798, Length=191, Percent_Identity=29.8429319371728, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI7706712, Length=233, Percent_Identity=30.4721030042918, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI308199517, Length=214, Percent_Identity=36.4485981308411, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17541892, Length=204, Percent_Identity=35.2941176470588, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71990482, Length=269, Percent_Identity=28.6245353159851, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17567771, Length=269, Percent_Identity=30.8550185873606, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71990487, Length=271, Percent_Identity=28.4132841328413, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17505510, Length=246, Percent_Identity=26.4227642276423, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325242, Length=258, Percent_Identity=36.8217054263566, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324504, Length=283, Percent_Identity=27.5618374558304, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320163, Length=258, Percent_Identity=26.7441860465116, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324599, Length=303, Percent_Identity=26.4026402640264, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137536, Length=242, Percent_Identity=32.2314049586777, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24648389, Length=204, Percent_Identity=37.2549019607843, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28571445, Length=260, Percent_Identity=30.7692307692308, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24645650, Length=240, Percent_Identity=28.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571443, Length=180, Percent_Identity=32.2222222222222, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571441, Length=180, Percent_Identity=32.2222222222222, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650933, Length=253, Percent_Identity=26.8774703557312, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27517; Mature: 27517

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEELAEEFLEFLDESESTAILTGAGVSTASGIPDFRGPQGLYKKLPQYIFDLDFFLSQ
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
PAEYYKIAADRIHNLFNKEPNATHRLLAMLEKKGMIEGVITQNIDGLHQKAGSKKVIELH
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHCCCCEEEEEC
GNAQKFFCMSCGKRYTAEDVLKMLEVSDVPKCTCGGLIKPDVVFFGEALPESAMAEAYIL
CCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHCCHHHHHHHHEE
SENAELFITMGSSLVVYPAAHLPTFAKQKGAKLLIINQGETGLDYIADKKYECDLAEFSE
ECCCEEEEEECCCEEEEECHHCCHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHH
RIISLLGEV
HHHHHHCCC
>Mature Secondary Structure
MNLEELAEEFLEFLDESESTAILTGAGVSTASGIPDFRGPQGLYKKLPQYIFDLDFFLSQ
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
PAEYYKIAADRIHNLFNKEPNATHRLLAMLEKKGMIEGVITQNIDGLHQKAGSKKVIELH
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHCCCCEEEEEC
GNAQKFFCMSCGKRYTAEDVLKMLEVSDVPKCTCGGLIKPDVVFFGEALPESAMAEAYIL
CCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHCCHHHHHHHHEE
SENAELFITMGSSLVVYPAAHLPTFAKQKGAKLLIINQGETGLDYIADKKYECDLAEFSE
ECCCEEEEEECCCEEEEECHHCCHHHHHCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHH
RIISLLGEV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]