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The map label for this gene is pilT [H]

Identifier: 239617234

GI number: 239617234

Start: 888775

End: 889830

Strand: Reverse

Name: pilT [H]

Synonym: Kole_0842

Alternate gene names: 239617234

Gene position: 889830-888775 (Counterclockwise)

Preceding gene: 239617239

Following gene: 239617233

Centisome position: 38.65

GC content: 41.48

Gene sequence:

>1056_bases
ATGCTTATCAATGAAATACTCGAAAAGGGTGAATTGTACAATGCTAGCGATATTCATATAACGACTGAAGAAAAATTGAA
ATATAGAGTAAATGGCGACCTCATTACCGATGAAACTCTTTTAATTGATCGTGAAAAAATGCTGGTTATACTTTCCGAAT
TAAAGGAACTAACGGGAATGGAGATGACCGATATTGATAAAAAAGAGATCGATTTCTCTTTTTCCTTCGGTCACTCGAGA
GTCAGAGGCAACCTCTTTTATGCCAACAAACTTCCCGCAATAGCCCTCAGGCTTATTCCAAGAACAATTAAAACAATAGA
TGAATGGGGATACCCAGCACTGTTCAAGGAATTCTGTACACCCGATAAAGGGTTGATACTCGTAGCTGGGCCAACGGGTA
GTGGTAAATCAACAACCCTTGCGGCAATGCTAGAATTTATAAACAGAACAAGAAGCGTCCATATCATAACAATTGAAGAT
CCCGTCGAATATGTCTTTGATCCTAAACACGCACTAATCCATCAACGAGAGCTTGGAGCGGATACAAATTCATTCGCCAA
TGGGTTGAAATACGCTTTGCGACAGGATCCAGACGTTATACTCGTTGGTGAGATGCGAGATCCAGAAACCATGGAACTTG
CGATGACCGCAGCTGAAACGGGACATCTTGTGTTCTCAACCATCCATACAAACTCCGCGGCTACCACGCCTGAAAGGATC
GTTGGAGTTTTTCCCCCGCACCAGCAGAACCAGATAGCTATGCAGTTAGCCAATACGCTTATAGCTGTAATATACCAGAG
GTTACTTAAAAGAAAAGATAAAAAGGGACGTATTCCGATAATCGAGATTATGGTTGTAAACCAGGCTATAAGGAACCTGA
TAAGAGAAAAGAAATTCCATCAGATCGAGTCTATGATGCAAGCGGGTGCGAAACTAGGAATGATAACCTTCGATGATGCG
CTTCTCAATGCTTACAAAAAAGGAATGATTGATTTAGAACAGGTCAAGAATTATGCCAGAGACCCAATTGCTATTTCCAG
GAGGCTAATGCGATGA

Upstream 100 bases:

>100_bases
CTCCTATAAACGCTTCTCATCCGATTTTAGCTTTTCATTAATACTTAACTGTTAAATCAATATGTTAAAATCCATAAGTC
AGAAGGAGGTGGGGGATTCT

Downstream 100 bases:

>100_bases
ATCTGGAAAAACTGATCGAACTCTTTAAGAAACAGCTTGAAGAGGTTGAAGCAAAATTGTCAGACTCTTCTATTGTAGGA
AATCCTTCGAAACTTATGGA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MLINEILEKGELYNASDIHITTEEKLKYRVNGDLITDETLLIDREKMLVILSELKELTGMEMTDIDKKEIDFSFSFGHSR
VRGNLFYANKLPAIALRLIPRTIKTIDEWGYPALFKEFCTPDKGLILVAGPTGSGKSTTLAAMLEFINRTRSVHIITIED
PVEYVFDPKHALIHQRELGADTNSFANGLKYALRQDPDVILVGEMRDPETMELAMTAAETGHLVFSTIHTNSAATTPERI
VGVFPPHQQNQIAMQLANTLIAVIYQRLLKRKDKKGRIPIIEIMVVNQAIRNLIREKKFHQIESMMQAGAKLGMITFDDA
LLNAYKKGMIDLEQVKNYARDPIAISRRLMR

Sequences:

>Translated_351_residues
MLINEILEKGELYNASDIHITTEEKLKYRVNGDLITDETLLIDREKMLVILSELKELTGMEMTDIDKKEIDFSFSFGHSR
VRGNLFYANKLPAIALRLIPRTIKTIDEWGYPALFKEFCTPDKGLILVAGPTGSGKSTTLAAMLEFINRTRSVHIITIED
PVEYVFDPKHALIHQRELGADTNSFANGLKYALRQDPDVILVGEMRDPETMELAMTAAETGHLVFSTIHTNSAATTPERI
VGVFPPHQQNQIAMQLANTLIAVIYQRLLKRKDKKGRIPIIEIMVVNQAIRNLIREKKFHQIESMMQAGAKLGMITFDDA
LLNAYKKGMIDLEQVKNYARDPIAISRRLMR
>Mature_351_residues
MLINEILEKGELYNASDIHITTEEKLKYRVNGDLITDETLLIDREKMLVILSELKELTGMEMTDIDKKEIDFSFSFGHSR
VRGNLFYANKLPAIALRLIPRTIKTIDEWGYPALFKEFCTPDKGLILVAGPTGSGKSTTLAAMLEFINRTRSVHIITIED
PVEYVFDPKHALIHQRELGADTNSFANGLKYALRQDPDVILVGEMRDPETMELAMTAAETGHLVFSTIHTNSAATTPERI
VGVFPPHQQNQIAMQLANTLIAVIYQRLLKRKDKKGRIPIIEIMVVNQAIRNLIREKKFHQIESMMQAGAKLGMITFDDA
LLNAYKKGMIDLEQVKNYARDPIAISRRLMR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=338, Percent_Identity=39.3491124260355, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI1789723, Length=295, Percent_Identity=38.6440677966102, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786296, Length=391, Percent_Identity=29.4117647058824, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39796; Mature: 39796

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLINEILEKGELYNASDIHITTEEKLKYRVNGDLITDETLLIDREKMLVILSELKELTGM
CCHHHHHHCCCCCCCCCEEEEEHHHEEEEECCCEEECCEEEECHHHHHHHHHHHHHHHCC
EMTDIDKKEIDFSFSFGHSRVRGNLFYANKLPAIALRLIPRTIKTIDEWGYPALFKEFCT
CCCCCCHHHCCEEEECCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
PDKGLILVAGPTGSGKSTTLAAMLEFINRTRSVHIITIEDPVEYVFDPKHALIHQRELGA
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCHHHHHHHHHCCC
DTNSFANGLKYALRQDPDVILVGEMRDPETMELAMTAAETGHLVFSTIHTNSAATTPERI
CHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEEHHCCCCEEEEEEECCCCCCCHHHE
VGVFPPHQQNQIAMQLANTLIAVIYQRLLKRKDKKGRIPIIEIMVVNQAIRNLIREKKFH
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
QIESMMQAGAKLGMITFDDALLNAYKKGMIDLEQVKNYARDPIAISRRLMR
HHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLINEILEKGELYNASDIHITTEEKLKYRVNGDLITDETLLIDREKMLVILSELKELTGM
CCHHHHHHCCCCCCCCCEEEEEHHHEEEEECCCEEECCEEEECHHHHHHHHHHHHHHHCC
EMTDIDKKEIDFSFSFGHSRVRGNLFYANKLPAIALRLIPRTIKTIDEWGYPALFKEFCT
CCCCCCHHHCCEEEECCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
PDKGLILVAGPTGSGKSTTLAAMLEFINRTRSVHIITIEDPVEYVFDPKHALIHQRELGA
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCHHHHHHHHHCCC
DTNSFANGLKYALRQDPDVILVGEMRDPETMELAMTAAETGHLVFSTIHTNSAATTPERI
CHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEEEHHCCCCEEEEEEECCCCCCCHHHE
VGVFPPHQQNQIAMQLANTLIAVIYQRLLKRKDKKGRIPIIEIMVVNQAIRNLIREKKFH
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
QIESMMQAGAKLGMITFDDALLNAYKKGMIDLEQVKNYARDPIAISRRLMR
HHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]