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The map label for this gene is prfA [H]

Identifier: 239617233

GI number: 239617233

Start: 887693

End: 888778

Strand: Reverse

Name: prfA [H]

Synonym: Kole_0841

Alternate gene names: 239617233

Gene position: 888778-887693 (Counterclockwise)

Preceding gene: 239617234

Following gene: 239617232

Centisome position: 38.61

GC content: 41.8

Gene sequence:

>1086_bases
ATGAATCTGGAAAAACTGATCGAACTCTTTAAGAAACAGCTTGAAGAGGTTGAAGCAAAATTGTCAGACTCTTCTATTGT
AGGAAATCCTTCGAAACTTATGGAACTAAGCAAACGCCATGCGGAACTCAAAGAGATTTCCAGAAGTTATGATCTTCTCG
CTTCTAAAAAACAGGAGTTACAGGAATGGGAGGAGATCAAAGAACTCTTATCACCTGAAGAAAGAGAAGAAGCGGAGAAA
AATATCGCTGAACTTCAAAAAGAAATTGAACAGATTGAACTCAACTTGAAATTTCTCCTGATTCCTGATGAACTTGGTGG
CAAAGATATTATCGTAGAAATACGAGCCGGTACAGGTGGTGAAGAGGCAGCGTTGTTCGCAGGTGATTTGTTCAGAATGT
ACACGCGCTATGCCGAACGAAACAGCTGGAAAGTTGAAACCCTTGAAAGCAATGAAACTGATTTGGGAGGCTTTAAGGAA
GTCACTTTTCAGATAAAGGGCAAAGATGTTTTTAGAAAGCTCAAGTACGAAAGCGGCGTTCATAGGGTTCAAAGAGTTCC
TCAAACAGAGTCTGGTGGAAGAATTCATACTTCTACTGCAACGGTTGCGGTATTGCCTAAGGCTTCAGAAGTAGATGTTG
CGATAGATCCTTCAGAACTCAGGATAGATACTTTCCGTTCTTCAGGAGCTGGAGGGCAACACGTTAACCGAACAGAATCG
GCGGTAAGAATTGTTCATCTTCCAACGGGAATAACTGTAACTGTTCAAAGCGAACGTTCGCAGCACCAGAACAAAGCGAG
AGCGATGGAGATATTGAGGGCAAGGCTTTATAGCTTGTACAGAAGTCAGCAGGAAAGCACACTTTCCCAACAAAGGCGTT
CGCAAATCGGTACCGGAGAAAGAAGCGAGAAAATCAGAACGTATAATTTCCCTCAAAATCGGGTTACAGATCACAGGATT
AATTACACAACCTACAGGCTTCAGGACATACTCGATGGTGATTTAGACGAATTGATTGCTAAATTGGCAGAAGCGGATAT
CAATATAAAATTGGAAAAACTTCTTGAGGAAATGACAACTGCGTGA

Upstream 100 bases:

>100_bases
ATGATGCGCTTCTCAATGCTTACAAAAAAGGAATGATTGATTTAGAACAGGTCAAGAATTATGCCAGAGACCCAATTGCT
ATTTCCAGGAGGCTAATGCG

Downstream 100 bases:

>100_bases
GGATTAACAAACTCGAGATCCGAGAAGGCTGCTTCGCAACGGATACGAATCCTTCGGGATTGACACGAACTCCTGCGGAG
TTATTTCGAACCACTTCGTG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNLEKLIELFKKQLEEVEAKLSDSSIVGNPSKLMELSKRHAELKEISRSYDLLASKKQELQEWEEIKELLSPEEREEAEK
NIAELQKEIEQIELNLKFLLIPDELGGKDIIVEIRAGTGGEEAALFAGDLFRMYTRYAERNSWKVETLESNETDLGGFKE
VTFQIKGKDVFRKLKYESGVHRVQRVPQTESGGRIHTSTATVAVLPKASEVDVAIDPSELRIDTFRSSGAGGQHVNRTES
AVRIVHLPTGITVTVQSERSQHQNKARAMEILRARLYSLYRSQQESTLSQQRRSQIGTGERSEKIRTYNFPQNRVTDHRI
NYTTYRLQDILDGDLDELIAKLAEADINIKLEKLLEEMTTA

Sequences:

>Translated_361_residues
MNLEKLIELFKKQLEEVEAKLSDSSIVGNPSKLMELSKRHAELKEISRSYDLLASKKQELQEWEEIKELLSPEEREEAEK
NIAELQKEIEQIELNLKFLLIPDELGGKDIIVEIRAGTGGEEAALFAGDLFRMYTRYAERNSWKVETLESNETDLGGFKE
VTFQIKGKDVFRKLKYESGVHRVQRVPQTESGGRIHTSTATVAVLPKASEVDVAIDPSELRIDTFRSSGAGGQHVNRTES
AVRIVHLPTGITVTVQSERSQHQNKARAMEILRARLYSLYRSQQESTLSQQRRSQIGTGERSEKIRTYNFPQNRVTDHRI
NYTTYRLQDILDGDLDELIAKLAEADINIKLEKLLEEMTTA
>Mature_361_residues
MNLEKLIELFKKQLEEVEAKLSDSSIVGNPSKLMELSKRHAELKEISRSYDLLASKKQELQEWEEIKELLSPEEREEAEK
NIAELQKEIEQIELNLKFLLIPDELGGKDIIVEIRAGTGGEEAALFAGDLFRMYTRYAERNSWKVETLESNETDLGGFKE
VTFQIKGKDVFRKLKYESGVHRVQRVPQTESGGRIHTSTATVAVLPKASEVDVAIDPSELRIDTFRSSGAGGQHVNRTES
AVRIVHLPTGITVTVQSERSQHQNKARAMEILRARLYSLYRSQQESTLSQQRRSQIGTGERSEKIRTYNFPQNRVTDHRI
NYTTYRLQDILDGDLDELIAKLAEADINIKLEKLLEEMTTA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=296, Percent_Identity=51.0135135135135, Blast_Score=295, Evalue=6e-80,
Organism=Homo sapiens, GI34577120, Length=368, Percent_Identity=39.4021739130435, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=199, Percent_Identity=50.251256281407, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1787462, Length=337, Percent_Identity=49.2581602373887, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=38.6904761904762, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=302, Percent_Identity=37.7483443708609, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=366, Percent_Identity=40.7103825136612, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921226, Length=294, Percent_Identity=45.2380952380952, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41188; Mature: 41188

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKLIELFKKQLEEVEAKLSDSSIVGNPSKLMELSKRHAELKEISRSYDLLASKKQEL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEWEEIKELLSPEEREEAEKNIAELQKEIEQIELNLKFLLIPDELGGKDIIVEIRAGTGG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCC
EEAALFAGDLFRMYTRYAERNSWKVETLESNETDLGGFKEVTFQIKGKDVFRKLKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
HRVQRVPQTESGGRIHTSTATVAVLPKASEVDVAIDPSELRIDTFRSSGAGGQHVNRTES
HHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCHHEEEHHHCCCCCCCCCCCCCC
AVRIVHLPTGITVTVQSERSQHQNKARAMEILRARLYSLYRSQQESTLSQQRRSQIGTGE
CEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSEKIRTYNFPQNRVTDHRINYTTYRLQDILDGDLDELIAKLAEADINIKLEKLLEEMTT
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHHHHHHC
A
C
>Mature Secondary Structure
MNLEKLIELFKKQLEEVEAKLSDSSIVGNPSKLMELSKRHAELKEISRSYDLLASKKQEL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEWEEIKELLSPEEREEAEKNIAELQKEIEQIELNLKFLLIPDELGGKDIIVEIRAGTGG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEEECCCCC
EEAALFAGDLFRMYTRYAERNSWKVETLESNETDLGGFKEVTFQIKGKDVFRKLKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
HRVQRVPQTESGGRIHTSTATVAVLPKASEVDVAIDPSELRIDTFRSSGAGGQHVNRTES
HHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCHHEEEHHHCCCCCCCCCCCCCC
AVRIVHLPTGITVTVQSERSQHQNKARAMEILRARLYSLYRSQQESTLSQQRRSQIGTGE
CEEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSEKIRTYNFPQNRVTDHRINYTTYRLQDILDGDLDELIAKLAEADINIKLEKLLEEMTT
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA