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The map label for this gene is cooF [H]

Identifier: 239616973

GI number: 239616973

Start: 621365

End: 621865

Strand: Reverse

Name: cooF [H]

Synonym: Kole_0572

Alternate gene names: 239616973

Gene position: 621865-621365 (Counterclockwise)

Preceding gene: 239616974

Following gene: 239616972

Centisome position: 27.01

GC content: 39.72

Gene sequence:

>501_bases
ATGAAGAAACTACTGATAGATATTGAGAAACTTATTAAGAGCGATATAAGCAGAATCAAATGTGAATACCTGTATCACGG
GAAAAATAATGGCGTTCTTTCGATTGTAGAGGTCGCGGAATTTGCGGTTTATTGCCGACAATGCAAGAACGCTCATTGTG
TGAAAGCATGTCCAAAGGATGCTTTAGAGAGACAACCGGATGGAACAGTAAAACGTTACAACCTGCTTTGTGTCGGTTGT
AAATCCTGTGTCCTTGCGTGTCCTTTTGGAACACTATTTCCAGAAACGATAAGTTACGTGACCACACATTGTGATTTCTG
TCTTTCTCAAATGAATGCTGATTCTAATTATGTTCCCTTATGTGCTGAAACCTGTAGTGGTAACACAATAAGGGTTTTAG
ATGTTGAAAGAGAACAACCTGAGAATGGATTTTATTTTGCCGGAAAGTATCTTGCGATACGCGCGCCGAATTGGCGTTAT
AAGGAGGGCAGAATAATTTGA

Upstream 100 bases:

>100_bases
TTGGACTTTGCAGACATTGCACCATAGAACACTATTTCGTTTGTAAAGATGGGCCAGTATTTACTTATGACCAATTTGAG
AATCCGGGGGAAATCTGGGC

Downstream 100 bases:

>100_bases
TTGTTTTTTATACCATTGTATTTCCGGGAATTCTCTTTACCGGTTTTGCAGGTTTAATAATATGGTGGCTGGAACGCAAG
CTTAGTGCTCTTTTCCAGCA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKKLLIDIEKLIKSDISRIKCEYLYHGKNNGVLSIVEVAEFAVYCRQCKNAHCVKACPKDALERQPDGTVKRYNLLCVGC
KSCVLACPFGTLFPETISYVTTHCDFCLSQMNADSNYVPLCAETCSGNTIRVLDVEREQPENGFYFAGKYLAIRAPNWRY
KEGRII

Sequences:

>Translated_166_residues
MKKLLIDIEKLIKSDISRIKCEYLYHGKNNGVLSIVEVAEFAVYCRQCKNAHCVKACPKDALERQPDGTVKRYNLLCVGC
KSCVLACPFGTLFPETISYVTTHCDFCLSQMNADSNYVPLCAETCSGNTIRVLDVEREQPENGFYFAGKYLAIRAPNWRY
KEGRII
>Mature_166_residues
MKKLLIDIEKLIKSDISRIKCEYLYHGKNNGVLSIVEVAEFAVYCRQCKNAHCVKACPKDALERQPDGTVKRYNLLCVGC
KSCVLACPFGTLFPETISYVTTHCDFCLSQMNADSNYVPLCAETCSGNTIRVLDVEREQPENGFYFAGKYLAIRAPNWRY
KEGRII

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082180, Length=92, Percent_Identity=39.1304347826087, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI2367245, Length=102, Percent_Identity=37.2549019607843, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1788811, Length=99, Percent_Identity=36.3636363636364, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18806; Mature: 18806

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
7.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLIDIEKLIKSDISRIKCEYLYHGKNNGVLSIVEVAEFAVYCRQCKNAHCVKACPKD
CCHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHCCCHH
ALERQPDGTVKRYNLLCVGCKSCVLACPFGTLFPETISYVTTHCDFCLSQMNADSNYVPL
HHHCCCCCCEEEEEEEEEECCHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
CAETCSGNTIRVLDVEREQPENGFYFAGKYLAIRAPNWRYKEGRII
EECCCCCCEEEEEECCCCCCCCCEEEECEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKLLIDIEKLIKSDISRIKCEYLYHGKNNGVLSIVEVAEFAVYCRQCKNAHCVKACPKD
CCHHHHHHHHHHHHHHHHEEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCHHHCCCHH
ALERQPDGTVKRYNLLCVGCKSCVLACPFGTLFPETISYVTTHCDFCLSQMNADSNYVPL
HHHCCCCCCEEEEEEEEEECCHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
CAETCSGNTIRVLDVEREQPENGFYFAGKYLAIRAPNWRYKEGRII
EECCCCCCEEEEEECCCCCCCCCEEEECEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]