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The map label for this gene is hycG [H]

Identifier: 239616971

GI number: 239616971

Start: 620041

End: 620454

Strand: Reverse

Name: hycG [H]

Synonym: Kole_0570

Alternate gene names: 239616971

Gene position: 620454-620041 (Counterclockwise)

Preceding gene: 239616972

Following gene: 239616970

Centisome position: 26.95

GC content: 44.93

Gene sequence:

>414_bases
ATGGGATTTTACACGAAGATTCTGAAAAGATCACTCTGGGTGTTTCATGTCAGCGCGTCACCATGTAATAACTGCGATAT
AGAGATTCTCGATATGTTAACGCCGAGATATGACCTCGAGCGTTTCGGAATAAAACTTGTCGGTTCAGTAAGACATGCTG
ACGTTCTGCTCGTAACTGGGATGGGGGGATGTAAGGTTTCTGAAAGGGTTATAAGGATTTATAACCAAGCTCCTAAGCCT
GTATTTGTGATCGCAGTAGGAACCTGCCCGGTGGCTGGAGCAACCTTTAAGGAGAGTTATACGAGGTGTGGACCTTTAGA
TAGATTTGTTCCTGTTGATTTGTACATACCAGGTTGTCCTCCAAAGCCAGAAACCATGATAGCCGGATTATCAGAACTGC
TTGGGAGGCTGTGA

Upstream 100 bases:

>100_bases
ACCCAAGGGTAACAATTGAAAAAATGCTCGGTTTTTTCTGGAAGAAACTGGCACCTATGGCTTTTATAGCGCTTCTCATA
GCACTTTTGGGGGGATGAAT

Downstream 100 bases:

>100_bases
AATGAATCCAATTGAAAGAAAAAAAGAGCTTTTTGCGGCGATCCAGGAAAATATTGAGCTTCTTTCTGAAAAGCGAAAGC
GTATTTACTTCAAAGTTGAT

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MGFYTKILKRSLWVFHVSASPCNNCDIEILDMLTPRYDLERFGIKLVGSVRHADVLLVTGMGGCKVSERVIRIYNQAPKP
VFVIAVGTCPVAGATFKESYTRCGPLDRFVPVDLYIPGCPPKPETMIAGLSELLGRL

Sequences:

>Translated_137_residues
MGFYTKILKRSLWVFHVSASPCNNCDIEILDMLTPRYDLERFGIKLVGSVRHADVLLVTGMGGCKVSERVIRIYNQAPKP
VFVIAVGTCPVAGATFKESYTRCGPLDRFVPVDLYIPGCPPKPETMIAGLSELLGRL
>Mature_136_residues
GFYTKILKRSLWVFHVSASPCNNCDIEILDMLTPRYDLERFGIKLVGSVRHADVLLVTGMGGCKVSERVIRIYNQAPKPV
FVIAVGTCPVAGATFKESYTRCGPLDRFVPVDLYIPGCPPKPETMIAGLSELLGRL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=109, Percent_Identity=39.4495412844037, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789074, Length=132, Percent_Identity=44.6969696969697, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1788834, Length=130, Percent_Identity=43.0769230769231, Blast_Score=113, Evalue=6e-27,
Organism=Escherichia coli, GI1788624, Length=123, Percent_Identity=36.5853658536585, Blast_Score=85, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=113, Percent_Identity=35.3982300884956, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24651058, Length=109, Percent_Identity=40.3669724770642, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18859983, Length=113, Percent_Identity=36.283185840708, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24642371, Length=113, Percent_Identity=36.283185840708, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 15118; Mature: 14987

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFYTKILKRSLWVFHVSASPCNNCDIEILDMLTPRYDLERFGIKLVGSVRHADVLLVTG
CCHHHHHHHCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHCEEEEECCCCCEEEEEEC
MGGCKVSERVIRIYNQAPKPVFVIAVGTCPVAGATFKESYTRCGPLDRFVPVDLYIPGCP
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
PKPETMIAGLSELLGRL
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFYTKILKRSLWVFHVSASPCNNCDIEILDMLTPRYDLERFGIKLVGSVRHADVLLVTG
CHHHHHHHCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHCEEEEECCCCCEEEEEEC
MGGCKVSERVIRIYNQAPKPVFVIAVGTCPVAGATFKESYTRCGPLDRFVPVDLYIPGCP
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
PKPETMIAGLSELLGRL
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]