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The map label for this gene is purH [H]

Identifier: 239616698

GI number: 239616698

Start: 316091

End: 317470

Strand: Direct

Name: purH [H]

Synonym: Kole_0291

Alternate gene names: 239616698

Gene position: 316091-317470 (Clockwise)

Preceding gene: 239616696

Following gene: 239616700

Centisome position: 13.73

GC content: 39.06

Gene sequence:

>1380_bases
TTGAATATCCACAAGGTTTTTGTAAGCGCCAGAATTACCGATGGTCTGGAAAACCTCTGTTCAAAACTGATATCAAAAGG
TGCTGAAATCATCGCCGATGAAAGCACCTACGCATTTCTTAAGTTGAAAAAGATCACTGTTAAAAACCTTCGGGAATTCT
TTGAAACCGAAGTAGCAGACGATTTTGCTCTTCAAAGAAGGATTGTTTCATGTTTGTCGGGAGCAAATGAAGAAAAAGCC
ACCTGTGGTAGTAATAATAAACACATTGATCTATTGGTGGTGAACTTCGAACCATTCTCCACATACGTTGAAAAGACCGG
CAACGAAACCGAATTGGTAGAACATATAGATTCTTTCAAGCCTTCACTGGTATTATCAGCAGCAAAAAATTATAGAAATG
TTGTGGTTTTGTGTGATCCTGAAGATTACGAAGACGTGCTCGAGGACCTCGAATATTACGGTGATGTGTCACTGCAGCGG
AGACGAAAACTGGCTCTGAAAGCGCTTTATCTTTTATCTGCATATACTTCGGAAATCTATAGTGTGTTCTCTGAGATCTT
TGCCGAGGAAAAATACAATTATCTTATTCTCGAAAAAATTATGCCACTTATGTTCGGAGAGATGTCGTTAAAAAAATCTA
CCCTTTACAAAGTGAGCGGAATTTGCGGGATTCTCGATGACGTCCACATAGCCAATCCATCAGTGGCCATGAATCACTCC
CATATTCGCAGCGTCCTCCAATTTATGAGGCTCTATTACTTCTTCGGAGACGTCTCAGCCTTTATATCAGACGGAAAATT
GATCGAGGTATCTTTAAGAGGCAACATGGAAACAATTCACCATGGTGAAAGCGTCATGTATGGTTCGTCATTTCCAATTA
GTTACCCAATGATAAAAAGATTATACGCAACAGGTGTAAAAAGCTTCTGTGGAGTATTCGAAAAAGAGGCTGTCAGATAT
GCTCACGATAACGATATCCTCCTGCTCACAATACCGGAAAGGAATTCTCTTTCAGTTGCAAAATTCAATTTTGTGGGTTT
TGAGAACTATATAGCCCTAGAAGAAAACAAATTCCCGAAAATGGATAGATTTTCCTCGACAGAAAAACTCGCAGTTATTA
CCTCATACTTTAATCCAAAAATTTCCCTCGCACTGGTTGAAACTGACAATGAACATTCTATAACGATCAATGTAGACTCT
GAAAACAGGCTCAGAACAGCTATTTTCGGATTTAATTCCTTGAATGGATATGTCCTGGCCTGTAATGTTGATCTGAGTGA
TGAAACGGTAGAACTTTTGAGATCAAAAGGAGTCAACAAAATGTATCTGCCATGCGTTAGCAAAACCTACAACTCAGTAG
ACATTATTCCTATAATCTGA

Upstream 100 bases:

>100_bases
AATACATGAACTATTGCTTCAAATTTACACAAATTCAGAGAAGAAAGTCAAGGAAAACACGTTCACGTTTGATAAAATTT
CTTTGACTGGGGGTGAAACT

Downstream 100 bases:

>100_bases
GTTCAAGCGGTTGAATATAACCCTTCGAGGTATTATAATGATTATGCACCATGCTAGGCGGGGAGCTGGCGGTTCCCTGT
ACCCGAAATCCGCCATATGC

Product: AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)-like protein

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNIHKVFVSARITDGLENLCSKLISKGAEIIADESTYAFLKLKKITVKNLREFFETEVADDFALQRRIVSCLSGANEEKA
TCGSNNKHIDLLVVNFEPFSTYVEKTGNETELVEHIDSFKPSLVLSAAKNYRNVVVLCDPEDYEDVLEDLEYYGDVSLQR
RRKLALKALYLLSAYTSEIYSVFSEIFAEEKYNYLILEKIMPLMFGEMSLKKSTLYKVSGICGILDDVHIANPSVAMNHS
HIRSVLQFMRLYYFFGDVSAFISDGKLIEVSLRGNMETIHHGESVMYGSSFPISYPMIKRLYATGVKSFCGVFEKEAVRY
AHDNDILLLTIPERNSLSVAKFNFVGFENYIALEENKFPKMDRFSSTEKLAVITSYFNPKISLALVETDNEHSITINVDS
ENRLRTAIFGFNSLNGYVLACNVDLSDETVELLRSKGVNKMYLPCVSKTYNSVDIIPII

Sequences:

>Translated_459_residues
MNIHKVFVSARITDGLENLCSKLISKGAEIIADESTYAFLKLKKITVKNLREFFETEVADDFALQRRIVSCLSGANEEKA
TCGSNNKHIDLLVVNFEPFSTYVEKTGNETELVEHIDSFKPSLVLSAAKNYRNVVVLCDPEDYEDVLEDLEYYGDVSLQR
RRKLALKALYLLSAYTSEIYSVFSEIFAEEKYNYLILEKIMPLMFGEMSLKKSTLYKVSGICGILDDVHIANPSVAMNHS
HIRSVLQFMRLYYFFGDVSAFISDGKLIEVSLRGNMETIHHGESVMYGSSFPISYPMIKRLYATGVKSFCGVFEKEAVRY
AHDNDILLLTIPERNSLSVAKFNFVGFENYIALEENKFPKMDRFSSTEKLAVITSYFNPKISLALVETDNEHSITINVDS
ENRLRTAIFGFNSLNGYVLACNVDLSDETVELLRSKGVNKMYLPCVSKTYNSVDIIPII
>Mature_459_residues
MNIHKVFVSARITDGLENLCSKLISKGAEIIADESTYAFLKLKKITVKNLREFFETEVADDFALQRRIVSCLSGANEEKA
TCGSNNKHIDLLVVNFEPFSTYVEKTGNETELVEHIDSFKPSLVLSAAKNYRNVVVLCDPEDYEDVLEDLEYYGDVSLQR
RRKLALKALYLLSAYTSEIYSVFSEIFAEEKYNYLILEKIMPLMFGEMSLKKSTLYKVSGICGILDDVHIANPSVAMNHS
HIRSVLQFMRLYYFFGDVSAFISDGKLIEVSLRGNMETIHHGESVMYGSSFPISYPMIKRLYATGVKSFCGVFEKEAVRY
AHDNDILLLTIPERNSLSVAKFNFVGFENYIALEENKFPKMDRFSSTEKLAVITSYFNPKISLALVETDNEHSITINVDS
ENRLRTAIFGFNSLNGYVLACNVDLSDETVELLRSKGVNKMYLPCVSKTYNSVDIIPII

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Escherichia coli, GI1790439, Length=102, Percent_Identity=35.2941176470588, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323056, Length=119, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=102, Percent_Identity=37.2549019607843, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 52023; Mature: 52023

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHKVFVSARITDGLENLCSKLISKGAEIIADESTYAFLKLKKITVKNLREFFETEVAD
CCHHEEEEEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHH
DFALQRRIVSCLSGANEEKATCGSNNKHIDLLVVNFEPFSTYVEKTGNETELVEHIDSFK
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHCCCCHHHHHHHHHHCC
PSLVLSAAKNYRNVVVLCDPEDYEDVLEDLEYYGDVSLQRRRKLALKALYLLSAYTSEIY
CHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVFSEIFAEEKYNYLILEKIMPLMFGEMSLKKSTLYKVSGICGILDDVHIANPSVAMNHS
HHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEECHH
HIRSVLQFMRLYYFFGDVSAFISDGKLIEVSLRGNMETIHHGESVMYGSSFPISYPMIKR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCEEEECCCCCCCHHHHHH
LYATGVKSFCGVFEKEAVRYAHDNDILLLTIPERNSLSVAKFNFVGFENYIALEENKFPK
HHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCC
MDRFSSTEKLAVITSYFNPKISLALVETDNEHSITINVDSENRLRTAIFGFNSLNGYVLA
CCCCCCCCEEEEEEEECCCEEEEEEEEECCCCEEEEEECCCCCEEEEEEEECCCCCEEEE
CNVDLSDETVELLRSKGVNKMYLPCVSKTYNSVDIIPII
EEECCCHHHHHHHHHCCCCEEEECCHHCCCCCEEEEEEC
>Mature Secondary Structure
MNIHKVFVSARITDGLENLCSKLISKGAEIIADESTYAFLKLKKITVKNLREFFETEVAD
CCHHEEEEEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEHHHHHHHHHHHHHHHHHH
DFALQRRIVSCLSGANEEKATCGSNNKHIDLLVVNFEPFSTYVEKTGNETELVEHIDSFK
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHCCCCHHHHHHHHHHCC
PSLVLSAAKNYRNVVVLCDPEDYEDVLEDLEYYGDVSLQRRRKLALKALYLLSAYTSEIY
CHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVFSEIFAEEKYNYLILEKIMPLMFGEMSLKKSTLYKVSGICGILDDVHIANPSVAMNHS
HHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEECHH
HIRSVLQFMRLYYFFGDVSAFISDGKLIEVSLRGNMETIHHGESVMYGSSFPISYPMIKR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCEEEECCCCCCCHHHHHH
LYATGVKSFCGVFEKEAVRYAHDNDILLLTIPERNSLSVAKFNFVGFENYIALEENKFPK
HHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCEEEEEEEECCCCEEEEECCCCCC
MDRFSSTEKLAVITSYFNPKISLALVETDNEHSITINVDSENRLRTAIFGFNSLNGYVLA
CCCCCCCCEEEEEEEECCCEEEEEEEEECCCCEEEEEECCCCCEEEEEEEECCCCCEEEE
CNVDLSDETVELLRSKGVNKMYLPCVSKTYNSVDIIPII
EEECCCHHHHHHHHHCCCCEEEECCHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA