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The map label for this gene is nfo [H]

Identifier: 239616680

GI number: 239616680

Start: 299067

End: 299933

Strand: Direct

Name: nfo [H]

Synonym: Kole_0273

Alternate gene names: 239616680

Gene position: 299067-299933 (Clockwise)

Preceding gene: 239616679

Following gene: 239616681

Centisome position: 12.99

GC content: 43.25

Gene sequence:

>867_bases
ATGATAATCGGTGCTCACATGTCAACATCCAGGGGGTTCGACAAAGTACCTCAAGCCAGTTTGAACATCGGCGGAAATAC
GTTTCAAATATTTCCCCACAGCCCATCAATGTGGCGAGCAAAACTTCCTGATGATACTACAGCGAGAAATTTTAAATACA
GGCTGGCGGAATCGGGTATAAAACCTGAAGATACGTTGGTTCACTCTGGATATTTAATAAACCTGGCCTCACCAAAACCC
GAAGTCTGGGAAAAATCAGTGGAACTGCTTGATTTAGAAATAAAAATCGTCGAAGTCCTTGGATTAAAATATCTGAATTT
TCATCCCGGAAGCCATCTTGGAACGGGTTTGGACGAAGGTATATCCAGAATCGCTAAAGGCATTGATGTGGTACTCTCAC
GCAATGAAGATAGCAAAGTTATCCTTCTCTTAGAAAACGTGGCACCAAAAGGCGGAAACATCGGCAGCACTTTCAATGAA
CTGCGCAGGATCATTGAGACATGTTCCTATCCCGAAAGGGTAATGATAACCTACGATACCTGCCACGGCTTCGATGCTGG
ATATGATATCCGAAGCAAAGCTGCCGTTGAAAAGCTTCTTGAAGAAATAGATTCCGAGGTCGGCCTTAATAGATTTGTTA
TGATCCACCTCAACGATTCTAAATATCCTCTTGGCGCGGCAAAAGATCGACATGAACGCATCGGGGAAGGTTACATCGGT
AATGAAGGTTTTTACCATTTCCTTTCAAACCCGGAGATACTAAAACGACCTTTAATACTGGAAACCCCGGGAAAAGATCC
AGAACATGCTGAAGACATAAAACGAGTAAAAGAGATCCTTGGAGAGAACGGTGATAGCGATGACTAA

Upstream 100 bases:

>100_bases
AAGAAAAGATTAAGGGAAATTGGAAGGGTTACAATACCGCAGACGGCATTCCTTGCTTTGTTAAAGATAGGGGAAGAAGA
TAAGTGAATGGGGTGAAATA

Downstream 100 bases:

>100_bases
AAAGTATATTGTTGTGACCGGTGGTGTGTTGAGTGGTATCGGGAAAGGAATTCTTTCAGCGTCACTTGCCAGGGTTTTGA
AGGAAGGCGGCGTTGACGTA

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MIIGAHMSTSRGFDKVPQASLNIGGNTFQIFPHSPSMWRAKLPDDTTARNFKYRLAESGIKPEDTLVHSGYLINLASPKP
EVWEKSVELLDLEIKIVEVLGLKYLNFHPGSHLGTGLDEGISRIAKGIDVVLSRNEDSKVILLLENVAPKGGNIGSTFNE
LRRIIETCSYPERVMITYDTCHGFDAGYDIRSKAAVEKLLEEIDSEVGLNRFVMIHLNDSKYPLGAAKDRHERIGEGYIG
NEGFYHFLSNPEILKRPLILETPGKDPEHAEDIKRVKEILGENGDSDD

Sequences:

>Translated_288_residues
MIIGAHMSTSRGFDKVPQASLNIGGNTFQIFPHSPSMWRAKLPDDTTARNFKYRLAESGIKPEDTLVHSGYLINLASPKP
EVWEKSVELLDLEIKIVEVLGLKYLNFHPGSHLGTGLDEGISRIAKGIDVVLSRNEDSKVILLLENVAPKGGNIGSTFNE
LRRIIETCSYPERVMITYDTCHGFDAGYDIRSKAAVEKLLEEIDSEVGLNRFVMIHLNDSKYPLGAAKDRHERIGEGYIG
NEGFYHFLSNPEILKRPLILETPGKDPEHAEDIKRVKEILGENGDSDD
>Mature_288_residues
MIIGAHMSTSRGFDKVPQASLNIGGNTFQIFPHSPSMWRAKLPDDTTARNFKYRLAESGIKPEDTLVHSGYLINLASPKP
EVWEKSVELLDLEIKIVEVLGLKYLNFHPGSHLGTGLDEGISRIAKGIDVVLSRNEDSKVILLLENVAPKGGNIGSTFNE
LRRIIETCSYPERVMITYDTCHGFDAGYDIRSKAAVEKLLEEIDSEVGLNRFVMIHLNDSKYPLGAAKDRHERIGEGYIG
NEGFYHFLSNPEILKRPLILETPGKDPEHAEDIKRVKEILGENGDSDD

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=276, Percent_Identity=36.231884057971, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531193, Length=270, Percent_Identity=36.6666666666667, Blast_Score=168, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322735, Length=268, Percent_Identity=34.3283582089552, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 32127; Mature: 32127

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGAHMSTSRGFDKVPQASLNIGGNTFQIFPHSPSMWRAKLPDDTTARNFKYRLAESGI
CEECCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHHHCCC
KPEDTLVHSGYLINLASPKPEVWEKSVELLDLEIKIVEVLGLKYLNFHPGSHLGTGLDEG
CCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHEEEEHHHCCHHEEECCCCCCCCCHHHH
ISRIAKGIDVVLSRNEDSKVILLLENVAPKGGNIGSTFNELRRIIETCSYPERVMITYDT
HHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
CHGFDAGYDIRSKAAVEKLLEEIDSEVGLNRFVMIHLNDSKYPLGAAKDRHERIGEGYIG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCC
NEGFYHFLSNPEILKRPLILETPGKDPEHAEDIKRVKEILGENGDSDD
CCCEEEECCCCHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIIGAHMSTSRGFDKVPQASLNIGGNTFQIFPHSPSMWRAKLPDDTTARNFKYRLAESGI
CEECCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHHHCCC
KPEDTLVHSGYLINLASPKPEVWEKSVELLDLEIKIVEVLGLKYLNFHPGSHLGTGLDEG
CCCCEEEECCEEEEECCCCHHHHHHHHHHHHHHEEEEHHHCCHHEEECCCCCCCCCHHHH
ISRIAKGIDVVLSRNEDSKVILLLENVAPKGGNIGSTFNELRRIIETCSYPERVMITYDT
HHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
CHGFDAGYDIRSKAAVEKLLEEIDSEVGLNRFVMIHLNDSKYPLGAAKDRHERIGEGYIG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCC
NEGFYHFLSNPEILKRPLILETPGKDPEHAEDIKRVKEILGENGDSDD
CCCEEEECCCCHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]