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The map label for this gene is pyrG [H]

Identifier: 239616681

GI number: 239616681

Start: 299917

End: 301521

Strand: Direct

Name: pyrG [H]

Synonym: Kole_0274

Alternate gene names: 239616681

Gene position: 299917-301521 (Clockwise)

Preceding gene: 239616680

Following gene: 239616682

Centisome position: 13.03

GC content: 43.55

Gene sequence:

>1605_bases
GTGATAGCGATGACTAAAAAGTATATTGTTGTGACCGGTGGTGTGTTGAGTGGTATCGGGAAAGGAATTCTTTCAGCGTC
ACTTGCCAGGGTTTTGAAGGAAGGCGGCGTTGACGTAAATGTTTTGAAGATTGATCCATACCTGAATGTTGACGCCGGTA
CGATGAATCCAAATCAACACGGAGAGGTTTTTGTAACGGAAGATGGATACGAAGCTGATCTCGATCTCGGACATTATGAA
AGATTCCTCGGTGTCAATATGCAAAGAATTAACAATGTAACGGCTGGTCAGATATACAAAAGTGTTGTTGATAAGGAACG
CGCCGGAAAATACCTCGGTGCTACCGTCCAAATGGTACCCCACGTTACAGCCGAGATTAAAGAGAGGATAGACAGAATCT
CCGGTGATCTCGTGTTGATTGAAATAGGCGGAACCGTCGGGGACATCGAGGGCGAAATATTCCTGGAAGCTGTCAGAGAG
ATGAGCCTTGAAAAAGGACCTGAGAATTTTCTCTTTATTCATGTCACTTATGTACCCTTCTTGAAAGCGTCCAACGAATT
CAAAACCAAACCCACACAACAGTCGGTACAGCTTTTGAGAAAGATAGGTATCCATCCGGACATGATCGCTGTTAGATCAG
AAATCCCCCTGGGAAACGACGAGTTGAATAAGATCGCCCTTTTTGGAGGAGTTCCGAAGCAAATGGTCTTTAACCTTCCA
GACGCCTCGAACGTTTACGAAATCCCCCACGTACTGTATTCAATGGGAATTCAGAAACTGATAGCACAAAAATTGGGAAT
AGAACTAAAGGATGTTTTCCACTGGGATTATCCGCGGGCTTTCAAACCATATCGCATAGCTCTTGTCGGTAAATACCTTG
GCACTGATGACGCCTACAAGAGCATAACGGAAAGTCTATTACTTTGTGGCGTCAGCAAACCGGAGACTGTTGATTCCCAA
ATACTTGAAGAAATGAGTGAAGATGAAATCGCTGTAACTCTCTCTTCATACGATGGAGTAATCATCCCCGGCGGTTTCGG
GAAACGTGGTATTGAAGGTAAGATAAAAGCGATAAAGTACGCAAGGCTCAACAATGTCCCGATCCTCGGGATTTGTCTGG
GGATGCAATTGATGGTCATCGAATTCGCAAGGAATGTAGCGGGTCTGGAAGGTGCGAATTCCAGAGAATTCGATCCAGAA
ACCCCATATCCCGTAATAGATTTGATGGAAGACCAGAAGAGGGTGCTCCAAATGGGAGGCACTATGAGATTGGGCGCTCA
AAAAACGCCTTTGTTTAAAGGTTCAAAGCTAAGAGAGATCTACGGAAAAGACCTCGTTATGGAACGTCACAGACACAGAT
ACGAGGTCAATTATGAAGAGTTCAAAAATCTCTTTACCTTTCCAGAAGATAAAGAAGCATTTAAAAATACCAGGCTTATT
ATCTCTTCAAAATTAGATTTTGTAGAGGCAATAGAACTACCAAATCACGTTTTCTTTGTTGGGATACAGTATCATCCTGA
ATTCAAATCAAGAGTGGGAGATCCCCACCCTCTCTTTAAAGCTTATGTTGAAGCGGTTGAAGCAAAAGGAGGGCATACAA
CTTGA

Upstream 100 bases:

>100_bases
CCGGAGATACTAAAACGACCTTTAATACTGGAAACCCCGGGAAAAGATCCAGAACATGCTGAAGACATAAAACGAGTAAA
AGAGATCCTTGGAGAGAACG

Downstream 100 bases:

>100_bases
AAAAGCTCCTGGCAAGAGGTCGTTATCTCGACCTTTCAGAAACAGTTGTAATGGGAATAATAAATGTTACCCCGGACTCG
TTCTACACCGGAAGCCGCTA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MIAMTKKYIVVTGGVLSGIGKGILSASLARVLKEGGVDVNVLKIDPYLNVDAGTMNPNQHGEVFVTEDGYEADLDLGHYE
RFLGVNMQRINNVTAGQIYKSVVDKERAGKYLGATVQMVPHVTAEIKERIDRISGDLVLIEIGGTVGDIEGEIFLEAVRE
MSLEKGPENFLFIHVTYVPFLKASNEFKTKPTQQSVQLLRKIGIHPDMIAVRSEIPLGNDELNKIALFGGVPKQMVFNLP
DASNVYEIPHVLYSMGIQKLIAQKLGIELKDVFHWDYPRAFKPYRIALVGKYLGTDDAYKSITESLLLCGVSKPETVDSQ
ILEEMSEDEIAVTLSSYDGVIIPGGFGKRGIEGKIKAIKYARLNNVPILGICLGMQLMVIEFARNVAGLEGANSREFDPE
TPYPVIDLMEDQKRVLQMGGTMRLGAQKTPLFKGSKLREIYGKDLVMERHRHRYEVNYEEFKNLFTFPEDKEAFKNTRLI
ISSKLDFVEAIELPNHVFFVGIQYHPEFKSRVGDPHPLFKAYVEAVEAKGGHTT

Sequences:

>Translated_534_residues
MIAMTKKYIVVTGGVLSGIGKGILSASLARVLKEGGVDVNVLKIDPYLNVDAGTMNPNQHGEVFVTEDGYEADLDLGHYE
RFLGVNMQRINNVTAGQIYKSVVDKERAGKYLGATVQMVPHVTAEIKERIDRISGDLVLIEIGGTVGDIEGEIFLEAVRE
MSLEKGPENFLFIHVTYVPFLKASNEFKTKPTQQSVQLLRKIGIHPDMIAVRSEIPLGNDELNKIALFGGVPKQMVFNLP
DASNVYEIPHVLYSMGIQKLIAQKLGIELKDVFHWDYPRAFKPYRIALVGKYLGTDDAYKSITESLLLCGVSKPETVDSQ
ILEEMSEDEIAVTLSSYDGVIIPGGFGKRGIEGKIKAIKYARLNNVPILGICLGMQLMVIEFARNVAGLEGANSREFDPE
TPYPVIDLMEDQKRVLQMGGTMRLGAQKTPLFKGSKLREIYGKDLVMERHRHRYEVNYEEFKNLFTFPEDKEAFKNTRLI
ISSKLDFVEAIELPNHVFFVGIQYHPEFKSRVGDPHPLFKAYVEAVEAKGGHTT
>Mature_534_residues
MIAMTKKYIVVTGGVLSGIGKGILSASLARVLKEGGVDVNVLKIDPYLNVDAGTMNPNQHGEVFVTEDGYEADLDLGHYE
RFLGVNMQRINNVTAGQIYKSVVDKERAGKYLGATVQMVPHVTAEIKERIDRISGDLVLIEIGGTVGDIEGEIFLEAVRE
MSLEKGPENFLFIHVTYVPFLKASNEFKTKPTQQSVQLLRKIGIHPDMIAVRSEIPLGNDELNKIALFGGVPKQMVFNLP
DASNVYEIPHVLYSMGIQKLIAQKLGIELKDVFHWDYPRAFKPYRIALVGKYLGTDDAYKSITESLLLCGVSKPETVDSQ
ILEEMSEDEIAVTLSSYDGVIIPGGFGKRGIEGKIKAIKYARLNNVPILGICLGMQLMVIEFARNVAGLEGANSREFDPE
TPYPVIDLMEDQKRVLQMGGTMRLGAQKTPLFKGSKLREIYGKDLVMERHRHRYEVNYEEFKNLFTFPEDKEAFKNTRLI
ISSKLDFVEAIELPNHVFFVGIQYHPEFKSRVGDPHPLFKAYVEAVEAKGGHTT

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI28559085, Length=549, Percent_Identity=44.08014571949, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI28559083, Length=549, Percent_Identity=44.08014571949, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI221316689, Length=549, Percent_Identity=44.08014571949, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=43.7956204379562, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1789142, Length=544, Percent_Identity=46.875, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25148299, Length=593, Percent_Identity=39.460370994941, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322563, Length=575, Percent_Identity=41.2173913043478, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=39.7188049209139, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24664469, Length=554, Percent_Identity=42.5992779783394, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI21357815, Length=495, Percent_Identity=42.020202020202, Blast_Score=338, Evalue=7e-93,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59618; Mature: 59618

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAMTKKYIVVTGGVLSGIGKGILSASLARVLKEGGVDVNVLKIDPYLNVDAGTMNPNQH
CCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCC
GEVFVTEDGYEADLDLGHYERFLGVNMQRINNVTAGQIYKSVVDKERAGKYLGATVQMVP
CCEEEECCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCHHHCHHHHHHH
HVTAEIKERIDRISGDLVLIEIGGTVGDIEGEIFLEAVREMSLEKGPENFLFIHVTYVPF
HHHHHHHHHHHHCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEH
LKASNEFKTKPTQQSVQLLRKIGIHPDMIAVRSEIPLGNDELNKIALFGGVPKQMVFNLP
HHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCHHHEEECC
DASNVYEIPHVLYSMGIQKLIAQKLGIELKDVFHWDYPRAFKPYRIALVGKYLGTDDAYK
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEHHHEECCCCCCCCCEEEEEEEHHHCCCHHHH
SITESLLLCGVSKPETVDSQILEEMSEDEIAVTLSSYDGVIIPGGFGKRGIEGKIKAIKY
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEE
ARLNNVPILGICLGMQLMVIEFARNVAGLEGANSREFDPETPYPVIDLMEDQKRVLQMGG
EECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
TMRLGAQKTPLFKGSKLREIYGKDLVMERHRHRYEVNYEEFKNLFTFPEDKEAFKNTRLI
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHCCCEEE
ISSKLDFVEAIELPNHVFFVGIQYHPEFKSRVGDPHPLFKAYVEAVEAKGGHTT
EECCCCHHHHHCCCCEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAMTKKYIVVTGGVLSGIGKGILSASLARVLKEGGVDVNVLKIDPYLNVDAGTMNPNQH
CCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCC
GEVFVTEDGYEADLDLGHYERFLGVNMQRINNVTAGQIYKSVVDKERAGKYLGATVQMVP
CCEEEECCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCHHHCHHHHHHH
HVTAEIKERIDRISGDLVLIEIGGTVGDIEGEIFLEAVREMSLEKGPENFLFIHVTYVPF
HHHHHHHHHHHHCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEH
LKASNEFKTKPTQQSVQLLRKIGIHPDMIAVRSEIPLGNDELNKIALFGGVPKQMVFNLP
HHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCHHHEEECC
DASNVYEIPHVLYSMGIQKLIAQKLGIELKDVFHWDYPRAFKPYRIALVGKYLGTDDAYK
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEHHHEECCCCCCCCCEEEEEEEHHHCCCHHHH
SITESLLLCGVSKPETVDSQILEEMSEDEIAVTLSSYDGVIIPGGFGKRGIEGKIKAIKY
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEE
ARLNNVPILGICLGMQLMVIEFARNVAGLEGANSREFDPETPYPVIDLMEDQKRVLQMGG
EECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
TMRLGAQKTPLFKGSKLREIYGKDLVMERHRHRYEVNYEEFKNLFTFPEDKEAFKNTRLI
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHCCCEEE
ISSKLDFVEAIELPNHVFFVGIQYHPEFKSRVGDPHPLFKAYVEAVEAKGGHTT
EECCCCHHHHHCCCCEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]