Click here to switch to the map view.

The map label for this gene is nuoF2 [H]

Identifier: 239616592

GI number: 239616592

Start: 205502

End: 207121

Strand: Direct

Name: nuoF2 [H]

Synonym: Kole_0184

Alternate gene names: 239616592

Gene position: 205502-207121 (Clockwise)

Preceding gene: 239616591

Following gene: 239616593

Centisome position: 8.93

GC content: 46.67

Gene sequence:

>1620_bases
ATGAAACCGATTACTGTGCTGGTCTCGGTGGATTCAAACAGCGTTTTGCTCGGTGCGAGGCACTTCAAGAATCATCTTGT
GGGTCTGGTGAAGAAATATAACCTGGACGGTATCGTTGAGGTGCTGGAAACAGGAAGCTTTGGGCGCTACGACGAAGGTG
CTCTATTCCTGCTTCTACCAGACAACATCTTCTACAAAATAAACTCTGAAGCAGACGTGGAAAGATTTGTTGAAGAGCAG
CTTTTGAAAGGCCGAAGGGTTGAAGATCTCATTGTAGAAGAGCTTGAAGGATCTGAAACCATCGAAGCCCGAACGATCAC
GAAGGAAGAGCGTATCGTTCTGAAACGTGCCGGCGAGATAAACCCGAAAAACATAGATGAATACATCGCTTTCGATGGTT
ATCAGGGTCTAGCAAAAGCCCTTCAAATGAGCCCTGAAGACGTTATAAAAGAAGTAAAGGAAAGCGGGCTCAGGGGACGT
GGTGGAGCCGGATTCCCCGCAGGATTGAAATGGGAGTTCACATACAAGGTTGATGCTAAAGAGAAGTACGTCATATGTAA
CGCCGATGAAGGTGAACCTGGTACCTTTAAAGACAGATTGATAATGGAAGGTGACCCCCACTCTGTTATAGAAGGTATGG
CCATCACCGCGTACGCTGTTGGCGCGAACAAGGGGTACATCTATATCAGAGGTGAATACTTTGAATCAGTCAACAATCTG
AGAATTGCCATTTCCCAGGCCTACGATTACGGATTGCTGGGTGAAAACATATTGGGCACCAACTTCTCCTTTGACCTATC
AGTTAGATTAGGTGCGGGTGCATACGTTTGTGGTGAAGAAACTGCCTTGATCGAGTCAATTGAAGGCCATGCTGGAAGGC
CGAGATTGAAACCACCGTATCCACCGGTGAAAGGCCTGTACCAGAAACCAACCGTAGTCAACAACGTTGAAACCTTCGCG
AACGTTCCGTGGATAGTCCTCAACGGCGCGGAATGGTTCCGTTCTATTGGAACGGAAAGTTCACCCGGTACCAAGGTGTA
CTCACTCTGTGGAAACCTCGTTAGAAGAGGAATCACGGAGGTACCTATGGGAACCACGGTCGCAGAACTGATCTACAAAC
ATGGTGGCGGAATAGCCAACGGAAGAAACCTTAAGATGGTTCAAACTGGTGGCGCTGCTGGTACTTTCATAAAACCAGAT
GAAATAAACACGCCACTGGATTTCAATTCTTTTAGAGATTACGGTGTCGCACTTGGTTCAGGTGTTATTCTGGCTGTTGA
TGACACTCACTGTGCCGTTGACGTGGCTCTGAACCTGATGGAATTCTTCGCTCACGAATCCTGTGGCAAATGTTCACCTT
GTCGTGAAGGTACAAAACTCATAGTCAATATTCTCAAAGAAATCAGCAAAGGTCGCGGAACAAAAGAAATGATTGGCCAG
CTTTATGACATAGCTGAAACGATGCAGAATTCGGCATTTTGTGGCCTTGGCCAATCCGTTCCGGTACCTCTCAGATCCCT
GCTTGATCGTTTCAGGGATGAGATTGAAGCCCATATTGGCGCCGAAAGCTGTCCTGTGGGTGTTTGTAAGTTCGAAAAGA
AAAAGAAGAAAAGAAAATAA

Upstream 100 bases:

>100_bases
CGGTAATGATGATCAATGATGAAGCTTACGGAAATCTGACTCCCGATAAAGTAGAAAAAATCATCAATGAAATACGCGAA
AAGGAAAGAAGTGATGCCAG

Downstream 100 bases:

>100_bases
TCTTTCCGTACAACTTTTTAACAAAAGAGGTCGGACTTCCCGGCCTCTTATTTTGATATAATACGACAAGTATGTCTGGA
GAAAAAAAGTACGATTTACC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKPITVLVSVDSNSVLLGARHFKNHLVGLVKKYNLDGIVEVLETGSFGRYDEGALFLLLPDNIFYKINSEADVERFVEEQ
LLKGRRVEDLIVEELEGSETIEARTITKEERIVLKRAGEINPKNIDEYIAFDGYQGLAKALQMSPEDVIKEVKESGLRGR
GGAGFPAGLKWEFTYKVDAKEKYVICNADEGEPGTFKDRLIMEGDPHSVIEGMAITAYAVGANKGYIYIRGEYFESVNNL
RIAISQAYDYGLLGENILGTNFSFDLSVRLGAGAYVCGEETALIESIEGHAGRPRLKPPYPPVKGLYQKPTVVNNVETFA
NVPWIVLNGAEWFRSIGTESSPGTKVYSLCGNLVRRGITEVPMGTTVAELIYKHGGGIANGRNLKMVQTGGAAGTFIKPD
EINTPLDFNSFRDYGVALGSGVILAVDDTHCAVDVALNLMEFFAHESCGKCSPCREGTKLIVNILKEISKGRGTKEMIGQ
LYDIAETMQNSAFCGLGQSVPVPLRSLLDRFRDEIEAHIGAESCPVGVCKFEKKKKKRK

Sequences:

>Translated_539_residues
MKPITVLVSVDSNSVLLGARHFKNHLVGLVKKYNLDGIVEVLETGSFGRYDEGALFLLLPDNIFYKINSEADVERFVEEQ
LLKGRRVEDLIVEELEGSETIEARTITKEERIVLKRAGEINPKNIDEYIAFDGYQGLAKALQMSPEDVIKEVKESGLRGR
GGAGFPAGLKWEFTYKVDAKEKYVICNADEGEPGTFKDRLIMEGDPHSVIEGMAITAYAVGANKGYIYIRGEYFESVNNL
RIAISQAYDYGLLGENILGTNFSFDLSVRLGAGAYVCGEETALIESIEGHAGRPRLKPPYPPVKGLYQKPTVVNNVETFA
NVPWIVLNGAEWFRSIGTESSPGTKVYSLCGNLVRRGITEVPMGTTVAELIYKHGGGIANGRNLKMVQTGGAAGTFIKPD
EINTPLDFNSFRDYGVALGSGVILAVDDTHCAVDVALNLMEFFAHESCGKCSPCREGTKLIVNILKEISKGRGTKEMIGQ
LYDIAETMQNSAFCGLGQSVPVPLRSLLDRFRDEIEAHIGAESCPVGVCKFEKKKKKRK
>Mature_539_residues
MKPITVLVSVDSNSVLLGARHFKNHLVGLVKKYNLDGIVEVLETGSFGRYDEGALFLLLPDNIFYKINSEADVERFVEEQ
LLKGRRVEDLIVEELEGSETIEARTITKEERIVLKRAGEINPKNIDEYIAFDGYQGLAKALQMSPEDVIKEVKESGLRGR
GGAGFPAGLKWEFTYKVDAKEKYVICNADEGEPGTFKDRLIMEGDPHSVIEGMAITAYAVGANKGYIYIRGEYFESVNNL
RIAISQAYDYGLLGENILGTNFSFDLSVRLGAGAYVCGEETALIESIEGHAGRPRLKPPYPPVKGLYQKPTVVNNVETFA
NVPWIVLNGAEWFRSIGTESSPGTKVYSLCGNLVRRGITEVPMGTTVAELIYKHGGGIANGRNLKMVQTGGAAGTFIKPD
EINTPLDFNSFRDYGVALGSGVILAVDDTHCAVDVALNLMEFFAHESCGKCSPCREGTKLIVNILKEISKGRGTKEMIGQ
LYDIAETMQNSAFCGLGQSVPVPLRSLLDRFRDEIEAHIGAESCPVGVCKFEKKKKKRK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=382, Percent_Identity=42.9319371727749, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI20149568, Length=382, Percent_Identity=42.9319371727749, Blast_Score=325, Evalue=7e-89,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=45.273631840796, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17531727, Length=375, Percent_Identity=41.8666666666667, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=42.4, Blast_Score=325, Evalue=7e-89,
Organism=Drosophila melanogaster, GI45550452, Length=375, Percent_Identity=41.3333333333333, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24653819, Length=391, Percent_Identity=37.0843989769821, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=34.9295774647887, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 59176; Mature: 59176

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPITVLVSVDSNSVLLGARHFKNHLVGLVKKYNLDGIVEVLETGSFGRYDEGALFLLLP
CCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEC
DNIFYKINSEADVERFVEEQLLKGRRVEDLIVEELEGSETIEARTITKEERIVLKRAGEI
CCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCC
NPKNIDEYIAFDGYQGLAKALQMSPEDVIKEVKESGLRGRGGAGFPAGLKWEFTYKVDAK
CCCCCHHHHEECCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCC
EKYVICNADEGEPGTFKDRLIMEGDPHSVIEGMAITAYAVGANKGYIYIRGEYFESVNNL
CEEEEEECCCCCCCCCCCCEEECCCCHHHHCCCEEEEEEEECCCCEEEEEEHHHHCCCCE
RIAISQAYDYGLLGENILGTNFSFDLSVRLGAGAYVCGEETALIESIEGHAGRPRLKPPY
EEEEEECCCCCCCCCCEECCCEEEEEEEEECCCEEEECCCHHHHHHHCCCCCCCCCCCCC
PPVKGLYQKPTVVNNVETFANVPWIVLNGAEWFRSIGTESSPGTKVYSLCGNLVRRGITE
CCCCCCCCCCCCCCCHHHHHCCCEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
VPMGTTVAELIYKHGGGIANGRNLKMVQTGGAAGTFIKPDEINTPLDFNSFRDYGVALGS
CCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCEEECC
GVILAVDDTHCAVDVALNLMEFFAHESCGKCSPCREGTKLIVNILKEISKGRGTKEMIGQ
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
LYDIAETMQNSAFCGLGQSVPVPLRSLLDRFRDEIEAHIGAESCPVGVCKFEKKKKKRK
HHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKPITVLVSVDSNSVLLGARHFKNHLVGLVKKYNLDGIVEVLETGSFGRYDEGALFLLLP
CCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEC
DNIFYKINSEADVERFVEEQLLKGRRVEDLIVEELEGSETIEARTITKEERIVLKRAGEI
CCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCC
NPKNIDEYIAFDGYQGLAKALQMSPEDVIKEVKESGLRGRGGAGFPAGLKWEFTYKVDAK
CCCCCHHHHEECCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCC
EKYVICNADEGEPGTFKDRLIMEGDPHSVIEGMAITAYAVGANKGYIYIRGEYFESVNNL
CEEEEEECCCCCCCCCCCCEEECCCCHHHHCCCEEEEEEEECCCCEEEEEEHHHHCCCCE
RIAISQAYDYGLLGENILGTNFSFDLSVRLGAGAYVCGEETALIESIEGHAGRPRLKPPY
EEEEEECCCCCCCCCCEECCCEEEEEEEEECCCEEEECCCHHHHHHHCCCCCCCCCCCCC
PPVKGLYQKPTVVNNVETFANVPWIVLNGAEWFRSIGTESSPGTKVYSLCGNLVRRGITE
CCCCCCCCCCCCCCCHHHHHCCCEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
VPMGTTVAELIYKHGGGIANGRNLKMVQTGGAAGTFIKPDEINTPLDFNSFRDYGVALGS
CCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCEEECC
GVILAVDDTHCAVDVALNLMEFFAHESCGKCSPCREGTKLIVNILKEISKGRGTKEMIGQ
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
LYDIAETMQNSAFCGLGQSVPVPLRSLLDRFRDEIEAHIGAESCPVGVCKFEKKKKKRK
HHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]