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The map label for this gene is hrb [H]

Identifier: 239616509

GI number: 239616509

Start: 115908

End: 116315

Strand: Direct

Name: hrb [H]

Synonym: Kole_0099

Alternate gene names: 239616509

Gene position: 115908-116315 (Clockwise)

Preceding gene: 239616508

Following gene: 239616511

Centisome position: 5.03

GC content: 40.93

Gene sequence:

>408_bases
ATGGATTATGAGGCTATGTTTAAGATCACTTACGGTATGTACGTTGTCACTTCAGAAACGAATGGGAAGAAAAATGGGCA
GATTGCTAATGTCGTGTTCCAGGTTGTAGCAGAACCACCGACAATAGCGATTTGCATAAACAAACAGAACTTAACCCATG
ATTTCATTCAGAAAAGCAAAGTATTTGGGGTGTCAGTTCTATCCCAAGATACCCCACTGAAGCTTATAGGGCACTTTGGA
TTTAAATCCGGAAGAGAGTTAGATAAGTTCAATGATATAAATCACAAAATAGGGGTAACCGGTGTCCCATTGTTAGAAGA
CCACTGCGTAGCGAACTTAGAGGCAAAAGTGGTAAACGCAGTTGATGTTGGTACCCATGTGGTTGGCTATGTCAAGAAAG
TGCTGTAA

Upstream 100 bases:

>100_bases
AAATATCTGACATCTGAAATTAGCAGAGCATTTTTGAATTACTGTTTAAACAAAGGGAACTGAGATTTTTTGTTTTAGTA
CCTGGTGCGGAGGTGAAAAG

Downstream 100 bases:

>100_bases
AAATAACCCTGTATCCTAAAACAGGCTAAGATCCCATGTTTAGATATCTCCTTCCAAGCAGCCATTCTTCGTTAATGTCA
AGTAAGATTGCGACAGCCAA

Product: flavin reductase domain protein FMN-binding

Products: NAD(+); reduced flavin [C]

Alternate protein names: Nitric oxide reductase NADH:FprA oxidoreductase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MDYEAMFKITYGMYVVTSETNGKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQKSKVFGVSVLSQDTPLKLIGHFG
FKSGRELDKFNDINHKIGVTGVPLLEDHCVANLEAKVVNAVDVGTHVVGYVKKVL

Sequences:

>Translated_135_residues
MDYEAMFKITYGMYVVTSETNGKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQKSKVFGVSVLSQDTPLKLIGHFG
FKSGRELDKFNDINHKIGVTGVPLLEDHCVANLEAKVVNAVDVGTHVVGYVKKVL
>Mature_135_residues
MDYEAMFKITYGMYVVTSETNGKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQKSKVFGVSVLSQDTPLKLIGHFG
FKSGRELDKFNDINHKIGVTGVPLLEDHCVANLEAKVVNAVDVGTHVVGYVKKVL

Specific function: Has nitric oxide reductase activity in combination with FprA; probably involved in nitrosative stress protection. Acts as an NADH:FprA oxidoreductase [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rubredoxin-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR004039
- InterPro:   IPR018527
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct; PF00301 Rubredoxin [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 14834; Mature: 14834

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYEAMFKITYGMYVVTSETNGKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQKSK
CCCHHEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
VFGVSVLSQDTPLKLIGHFGFKSGRELDKFNDINHKIGVTGVPLLEDHCVANLEAKVVNA
HEEEEEECCCCCEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHCCCHHEEEEH
VDVGTHVVGYVKKVL
HHHHHHHHHHHHHHC
>Mature Secondary Structure
MDYEAMFKITYGMYVVTSETNGKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQKSK
CCCHHEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
VFGVSVLSQDTPLKLIGHFGFKSGRELDKFNDINHKIGVTGVPLLEDHCVANLEAKVVNA
HEEEEEECCCCCEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHCCCHHEEEEH
VDVGTHVVGYVKKVL
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11160086; 12627946 [H]