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The map label for this gene is nplT [H]

Identifier: 238925638

GI number: 238925638

Start: 3129932

End: 3131650

Strand: Reverse

Name: nplT [H]

Synonym: EUBREC_3295

Alternate gene names: 238925638

Gene position: 3131650-3129932 (Counterclockwise)

Preceding gene: 238925639

Following gene: 238925637

Centisome position: 90.78

GC content: 42.35

Gene sequence:

>1719_bases
ATGGAATATTCAGCAATTTTTCATGACATGGATAAAAGATTCTGCTATGCGATAGATAAGGATTTGTTTGTGATACGCGT
GCAGGTAAAAAAGGATGATATGAAGGAGGTAATCCTTCACTATGAGGACAAGTATATACCTATGGAGCGCAAGGATACGC
GAATGACACTTCCTATGAAGAAGGTAGCCACCTCGCAGTTTCACGATTATTATGAAGCACAGCTTCAGATGCATCTGATA
TGTCTGAGATACTTTTTTGAATTTACAGACATGCAGGGTGAAAAGGTATATTACGGCAATTATGAGTTCGACAAGGAGTG
CATCACAAACAGGGACAGAATGTTTGACTGCCCGCAGAACCTCAGGGAGGAAGAGATGTTTGAGGTGCCACAGTGGGCAG
CAAACAAGGTAGTGTATCAGATTTTCCCGTCGCGCTTTGCCGCAACACAGCCGGTAGACAAAAAGCTGTGGTATAAGGCT
CCGATTACGCCTATGGATGATCTGCATGGCAATCTCAGAGGAATCATAGAGCATCTGGATTATATTAAAGATTTGGGAAT
CGATGTGGTGTATCTGACACCAATCTTTAAATCAAATTCGTGCCATAAGTATGATACCATTGATTATTATCAGGTTGATC
CATCATTTGGAACAACAGAGGATTTAAAGGAGCTCGTACAAAAATCTCACGAGCGTGGCATGAAGGTAGTGCTGGATGCG
GTATACAATCACACAGGAAGAGAATTTTTTGCATTTCAGGATATACTTGAAAAGGGCGAAAAGTCAAAATATCTTGACTG
GTATTTTATCGACGAGCTGCCGCCTAGGGGTGAATGGGGAGAAATTCCGAACTTCAAATGCTTCGGCTATTACGGTGGAA
TGCCAAAGCTTAATCTGAAAAATCCTGAGGTTGAAAAATACATCACGGATGTTGCCTGCTACTGGATTAAGGAATGTGAT
ATTGACGGATGGAGACTGGATGTAGGAGACGAAATCAGTCATTTCTTCTGGAAGAATTTCAGAAAGGCAATCAAGGCAGT
AAAGAAGGATATGCTGATAATTGGTGAGATATGGCACTACGCAGGAGACTTCCTCGAGGGAGATGAGTGGGATACTGTCA
TGAATTATCCATTCTACCTGAATCTGATAGACCTGCTTGCAGACGAAAAGATTAATGTCAGCCAGTTTGTACAAAATCTT
GGATATCTGAAGGGACGCTTAAACAAAAAGTGCTATCCGCTGATGTGGAACCTGATTGACAGCCACGATACAGCAAGATT
TTTACATCTGTGCCATGATAATAAGAAAAAGCAGCATCTCGCAGCAGCCTTTCAGCTGCTACTGCCGGGAATGCCTATGG
TTTACTATGGCGATGAGTATGCCATGCCGGGAGCAAACGATCCGGACTGCAGACGAGGCATGTACTGGGATGAAGAATAT
CAGGACAAGGAGATGTATAATTGGTATAAGAAGCTCATGCAGGTTAGAAAAACACATGCATGCATTGTAGAGGGCGAAAT
GATTGAGACAATCACAAATGACGATGATGATACGATTGTTATGATCAGAAAAAACGGCGAAGAGACAATCGCAATGCTTT
TCAATTGCGGCAGCAGTGTAAAGGAATTTAATGAGTATGCCGAGAAATATAATCTGCTGACAGATAGCGCATTTGACGGA
AAAGTAGAGGGGCTTGACGCTGCCGTAATTGTGCTTTAA

Upstream 100 bases:

>100_bases
GTAGAAATAGTGTAAAAATGTCATGGATATAATGATAGAATTGTTTGAAAAAGTGCGTTAAAATACAAAATAACGAAATA
CGTAAGTAAAGGGGCATTTT

Downstream 100 bases:

>100_bases
AAATTATATGGACAGGAAATTGCAGGCAGCTGCTTTTTCCTGTTTTTTTGCATAAAATCAATTCTGTTGAGGTAAAATTG
AGATTGACATTGTATTGTAT

Product: alpha-amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MEYSAIFHDMDKRFCYAIDKDLFVIRVQVKKDDMKEVILHYEDKYIPMERKDTRMTLPMKKVATSQFHDYYEAQLQMHLI
CLRYFFEFTDMQGEKVYYGNYEFDKECITNRDRMFDCPQNLREEEMFEVPQWAANKVVYQIFPSRFAATQPVDKKLWYKA
PITPMDDLHGNLRGIIEHLDYIKDLGIDVVYLTPIFKSNSCHKYDTIDYYQVDPSFGTTEDLKELVQKSHERGMKVVLDA
VYNHTGREFFAFQDILEKGEKSKYLDWYFIDELPPRGEWGEIPNFKCFGYYGGMPKLNLKNPEVEKYITDVACYWIKECD
IDGWRLDVGDEISHFFWKNFRKAIKAVKKDMLIIGEIWHYAGDFLEGDEWDTVMNYPFYLNLIDLLADEKINVSQFVQNL
GYLKGRLNKKCYPLMWNLIDSHDTARFLHLCHDNKKKQHLAAAFQLLLPGMPMVYYGDEYAMPGANDPDCRRGMYWDEEY
QDKEMYNWYKKLMQVRKTHACIVEGEMIETITNDDDDTIVMIRKNGEETIAMLFNCGSSVKEFNEYAEKYNLLTDSAFDG
KVEGLDAAVIVL

Sequences:

>Translated_572_residues
MEYSAIFHDMDKRFCYAIDKDLFVIRVQVKKDDMKEVILHYEDKYIPMERKDTRMTLPMKKVATSQFHDYYEAQLQMHLI
CLRYFFEFTDMQGEKVYYGNYEFDKECITNRDRMFDCPQNLREEEMFEVPQWAANKVVYQIFPSRFAATQPVDKKLWYKA
PITPMDDLHGNLRGIIEHLDYIKDLGIDVVYLTPIFKSNSCHKYDTIDYYQVDPSFGTTEDLKELVQKSHERGMKVVLDA
VYNHTGREFFAFQDILEKGEKSKYLDWYFIDELPPRGEWGEIPNFKCFGYYGGMPKLNLKNPEVEKYITDVACYWIKECD
IDGWRLDVGDEISHFFWKNFRKAIKAVKKDMLIIGEIWHYAGDFLEGDEWDTVMNYPFYLNLIDLLADEKINVSQFVQNL
GYLKGRLNKKCYPLMWNLIDSHDTARFLHLCHDNKKKQHLAAAFQLLLPGMPMVYYGDEYAMPGANDPDCRRGMYWDEEY
QDKEMYNWYKKLMQVRKTHACIVEGEMIETITNDDDDTIVMIRKNGEETIAMLFNCGSSVKEFNEYAEKYNLLTDSAFDG
KVEGLDAAVIVL
>Mature_572_residues
MEYSAIFHDMDKRFCYAIDKDLFVIRVQVKKDDMKEVILHYEDKYIPMERKDTRMTLPMKKVATSQFHDYYEAQLQMHLI
CLRYFFEFTDMQGEKVYYGNYEFDKECITNRDRMFDCPQNLREEEMFEVPQWAANKVVYQIFPSRFAATQPVDKKLWYKA
PITPMDDLHGNLRGIIEHLDYIKDLGIDVVYLTPIFKSNSCHKYDTIDYYQVDPSFGTTEDLKELVQKSHERGMKVVLDA
VYNHTGREFFAFQDILEKGEKSKYLDWYFIDELPPRGEWGEIPNFKCFGYYGGMPKLNLKNPEVEKYITDVACYWIKECD
IDGWRLDVGDEISHFFWKNFRKAIKAVKKDMLIIGEIWHYAGDFLEGDEWDTVMNYPFYLNLIDLLADEKINVSQFVQNL
GYLKGRLNKKCYPLMWNLIDSHDTARFLHLCHDNKKKQHLAAAFQLLLPGMPMVYYGDEYAMPGANDPDCRRGMYWDEEY
QDKEMYNWYKKLMQVRKTHACIVEGEMIETITNDDDDTIVMIRKNGEETIAMLFNCGSSVKEFNEYAEKYNLLTDSAFDG
KVEGLDAAVIVL

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=218, Percent_Identity=29.3577981651376, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1786604, Length=582, Percent_Identity=28.0068728522337, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1790687, Length=224, Percent_Identity=32.5892857142857, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789995, Length=508, Percent_Identity=21.0629921259843, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32565753, Length=409, Percent_Identity=23.2273838630807, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322241, Length=225, Percent_Identity=30.6666666666667, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322021, Length=225, Percent_Identity=30.6666666666667, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324416, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321726, Length=225, Percent_Identity=31.1111111111111, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321731, Length=224, Percent_Identity=29.0178571428571, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=224, Percent_Identity=29.0178571428571, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24586587, Length=219, Percent_Identity=29.6803652968037, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583745, Length=216, Percent_Identity=29.6296296296296, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583747, Length=226, Percent_Identity=29.646017699115, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583749, Length=226, Percent_Identity=29.646017699115, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24586591, Length=218, Percent_Identity=29.8165137614679, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24586599, Length=218, Percent_Identity=30.7339449541284, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI221330053, Length=180, Percent_Identity=31.6666666666667, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45549022, Length=220, Percent_Identity=27.7272727272727, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586589, Length=221, Percent_Identity=29.8642533936652, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24586593, Length=228, Percent_Identity=27.1929824561404, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24586597, Length=218, Percent_Identity=28.8990825688073, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 67753; Mature: 67753

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYSAIFHDMDKRFCYAIDKDLFVIRVQVKKDDMKEVILHYEDKYIPMERKDTRMTLPMK
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEECCHH
KVATSQFHDYYEAQLQMHLICLRYFFEFTDMQGEKVYYGNYEFDKECITNRDRMFDCPQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCCCCCHH
LREEEMFEVPQWAANKVVYQIFPSRFAATQPVDKKLWYKAPITPMDDLHGNLRGIIEHLD
CCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCHHHHCCHHHHHHHHHH
YIKDLGIDVVYLTPIFKSNSCHKYDTIDYYQVDPSFGTTEDLKELVQKSHERGMKVVLDA
HHHHCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHH
VYNHTGREFFAFQDILEKGEKSKYLDWYFIDELPPRGEWGEIPNFKCFGYYGGMPKLNLK
HHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCC
NPEVEKYITDVACYWIKECDIDGWRLDVGDEISHFFWKNFRKAIKAVKKDMLIIGEIWHY
CCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDFLEGDEWDTVMNYPFYLNLIDLLADEKINVSQFVQNLGYLKGRLNKKCYPLMWNLID
HCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHC
SHDTARFLHLCHDNKKKQHLAAAFQLLLPGMPMVYYGDEYAMPGANDPDCRRGMYWDEEY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHH
QDKEMYNWYKKLMQVRKTHACIVEGEMIETITNDDDDTIVMIRKNGEETIAMLFNCGSSV
CCHHHHHHHHHHHHHHHHHHEEECCHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCHH
KEFNEYAEKYNLLTDSAFDGKVEGLDAAVIVL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MEYSAIFHDMDKRFCYAIDKDLFVIRVQVKKDDMKEVILHYEDKYIPMERKDTRMTLPMK
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEECCHH
KVATSQFHDYYEAQLQMHLICLRYFFEFTDMQGEKVYYGNYEFDKECITNRDRMFDCPQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCCCCCHH
LREEEMFEVPQWAANKVVYQIFPSRFAATQPVDKKLWYKAPITPMDDLHGNLRGIIEHLD
CCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCHHHHCCHHHHHHHHHH
YIKDLGIDVVYLTPIFKSNSCHKYDTIDYYQVDPSFGTTEDLKELVQKSHERGMKVVLDA
HHHHCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHH
VYNHTGREFFAFQDILEKGEKSKYLDWYFIDELPPRGEWGEIPNFKCFGYYGGMPKLNLK
HHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCC
NPEVEKYITDVACYWIKECDIDGWRLDVGDEISHFFWKNFRKAIKAVKKDMLIIGEIWHY
CCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDFLEGDEWDTVMNYPFYLNLIDLLADEKINVSQFVQNLGYLKGRLNKKCYPLMWNLID
HCCCCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHC
SHDTARFLHLCHDNKKKQHLAAAFQLLLPGMPMVYYGDEYAMPGANDPDCRRGMYWDEEY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHH
QDKEMYNWYKKLMQVRKTHACIVEGEMIETITNDDDDTIVMIRKNGEETIAMLFNCGSSV
CCHHHHHHHHHHHHHHHHHHEEECCHHHHHHCCCCCCEEEEEEECCCHHHHHHHHCCCHH
KEFNEYAEKYNLLTDSAFDGKVEGLDAAVIVL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]