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The map label for this gene is yaaJ [C]

Identifier: 238925517

GI number: 238925517

Start: 3023925

End: 3025352

Strand: Reverse

Name: yaaJ [C]

Synonym: EUBREC_3173

Alternate gene names: 238925517

Gene position: 3025352-3023925 (Counterclockwise)

Preceding gene: 238925518

Following gene: 238925516

Centisome position: 87.7

GC content: 46.29

Gene sequence:

>1428_bases
ATGTGGAACACAATCAACAATTTTCTTAGTGCAGTGGACAATTTCGTCTGGGGAGTGCCGCTCATGGTGCTCATCATGGC
AGGAGGTATAATGCTCACAGTTCGTCTGGGACTTCTTCAGATGAGAAAGCTTCCTCTGGCACTAAAGTGGATGGTCAAAA
ATGAAGAGGGCGGCGATGGCGAGATTACGAGCTTTGGCGCACTCTGTACCGCGCTTAGCGCAACAATCGGAACCGGCAAC
ATAGTCGGTGTAGCAACTGCAGTCGGTGCAGGAGGACCGGGAGCGCTTTTTTGGATGGTGCTTGCAGCCTTCTTTGGCAT
GGCAACAAAGTATTCAGAGGGACTTTTGGCTGTAAAATATCGTGTCATCGGCAAGGACGGACATAGTCTCGGCGGACCAT
TCTACTACATAGAGCAGGGAATGGGTGCAAAATGGAAATGGCTTGCAAAGATATTTGCATTTTTCGGCGTATGCGTTGGT
CTTTTCGGAATAGGAACATTCAGCCAGGTAAACGGAATATCAAGTGCTGTAAACAACTTTTTTGACCCTAAAAACCAGCA
TACAGTAAAGGTTTTACCGTTCCTTGGTGAGTATTCATGGTCGGTTGTCATTGCTTCGCTTGTACTGGCATTCTGCGTGG
CAGCAGTGCTTATCGGCGGAGTAAAGCGTATTGCAAGTGTCAGCCAGATAATAGTTCCTTTTATGGCTGTTATATATATT
GTATTTGTGCTCGTGCTTGTGGTATGCAATATCACAGCGGTTCCGGCAGCGTTTGCTACAATAGTAAAGGCAGCGTTTTC
ACCGAAGGCCATAACAGGCGGTGCCGTGGGAAGCTTTCTTGTTGCAATGCAAAAGGGTGTTGCCAGAGGAATCTTTTCAA
ACGAGGCAGGACTTGGTAGTGCCCCGATTGCAGCGGCAGCAGCCCAGACAAAGGAGCCTGTACGTCAGGGTCTTGTCAGC
ATGACAGGAACCTTCATTGATACAATAGTTATATGTACACTTACAGGACTTTCCATCGTGCTCACAGGCGCATGGCAGGT
TGACGGACTCGAGGGCGTGCAGGTTACTACATACGCATTCCAGAATGGTCTTCCTTTACCGAAAGAGTTATCAGCATTTG
TACTTATGCTGTGTCTTGTATTTTTTGCCTTTACAACTATACTCGGTTGGGATTATTATAGTGAGCGGTGCTTAGAGTAT
TTGAGTGGCGGAAGGATGAAATATGTAAAGGTTTATCGCTGGATATATATACTTGCAGTTTTCATCGGACCTTATATGAC
TGTAAGTGCGGTGTGGACTATCGCTGATATCTTCAACGGTCTCATGGCTCTGCCAAATATGATAGCGCTGTTTGCTCTTA
GTGGTGTTGTTGTTAAGGAGACAAGGCATTTCTTCGAGAGACATAGAAATGGAGAAATCGAGGATTAA

Upstream 100 bases:

>100_bases
TTTTTCAATTGTTTCCAGATGAAATTATTTATATCAATAGTATTTGCTCAAAACACAAAGATCTTGTGTTTAGATAAAAA
AGAGAGGCAGGAGTATTTCA

Downstream 100 bases:

>100_bases
TTATGTTTTATATTTTGCGACATGGAAGAACAGATTGGAATGAGGAGCACAGACTGCAGGGTGAGGTGGATATCCCACTC
AACGAAACCGGCAGGCAGAT

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MWNTINNFLSAVDNFVWGVPLMVLIMAGGIMLTVRLGLLQMRKLPLALKWMVKNEEGGDGEITSFGALCTALSATIGTGN
IVGVATAVGAGGPGALFWMVLAAFFGMATKYSEGLLAVKYRVIGKDGHSLGGPFYYIEQGMGAKWKWLAKIFAFFGVCVG
LFGIGTFSQVNGISSAVNNFFDPKNQHTVKVLPFLGEYSWSVVIASLVLAFCVAAVLIGGVKRIASVSQIIVPFMAVIYI
VFVLVLVVCNITAVPAAFATIVKAAFSPKAITGGAVGSFLVAMQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVS
MTGTFIDTIVICTLTGLSIVLTGAWQVDGLEGVQVTTYAFQNGLPLPKELSAFVLMLCLVFFAFTTILGWDYYSERCLEY
LSGGRMKYVKVYRWIYILAVFIGPYMTVSAVWTIADIFNGLMALPNMIALFALSGVVVKETRHFFERHRNGEIED

Sequences:

>Translated_475_residues
MWNTINNFLSAVDNFVWGVPLMVLIMAGGIMLTVRLGLLQMRKLPLALKWMVKNEEGGDGEITSFGALCTALSATIGTGN
IVGVATAVGAGGPGALFWMVLAAFFGMATKYSEGLLAVKYRVIGKDGHSLGGPFYYIEQGMGAKWKWLAKIFAFFGVCVG
LFGIGTFSQVNGISSAVNNFFDPKNQHTVKVLPFLGEYSWSVVIASLVLAFCVAAVLIGGVKRIASVSQIIVPFMAVIYI
VFVLVLVVCNITAVPAAFATIVKAAFSPKAITGGAVGSFLVAMQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVS
MTGTFIDTIVICTLTGLSIVLTGAWQVDGLEGVQVTTYAFQNGLPLPKELSAFVLMLCLVFFAFTTILGWDYYSERCLEY
LSGGRMKYVKVYRWIYILAVFIGPYMTVSAVWTIADIFNGLMALPNMIALFALSGVVVKETRHFFERHRNGEIED
>Mature_475_residues
MWNTINNFLSAVDNFVWGVPLMVLIMAGGIMLTVRLGLLQMRKLPLALKWMVKNEEGGDGEITSFGALCTALSATIGTGN
IVGVATAVGAGGPGALFWMVLAAFFGMATKYSEGLLAVKYRVIGKDGHSLGGPFYYIEQGMGAKWKWLAKIFAFFGVCVG
LFGIGTFSQVNGISSAVNNFFDPKNQHTVKVLPFLGEYSWSVVIASLVLAFCVAAVLIGGVKRIASVSQIIVPFMAVIYI
VFVLVLVVCNITAVPAAFATIVKAAFSPKAITGGAVGSFLVAMQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVS
MTGTFIDTIVICTLTGLSIVLTGAWQVDGLEGVQVTTYAFQNGLPLPKELSAFVLMLCLVFFAFTTILGWDYYSERCLEY
LSGGRMKYVKVYRWIYILAVFIGPYMTVSAVWTIADIFNGLMALPNMIALFALSGVVVKETRHFFERHRNGEIED

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=471, Percent_Identity=33.3333333333333, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 50984; Mature: 50984

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNTINNFLSAVDNFVWGVPLMVLIMAGGIMLTVRLGLLQMRKLPLALKWMVKNEEGGDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCC
EITSFGALCTALSATIGTGNIVGVATAVGAGGPGALFWMVLAAFFGMATKYSEGLLAVKY
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
RVIGKDGHSLGGPFYYIEQGMGAKWKWLAKIFAFFGVCVGLFGIGTFSQVNGISSAVNNF
EEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
FDPKNQHTVKVLPFLGEYSWSVVIASLVLAFCVAAVLIGGVKRIASVSQIIVPFMAVIYI
CCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVLVLVVCNITAVPAAFATIVKAAFSPKAITGGAVGSFLVAMQKGVARGIFSNEAGLGS
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
APIAAAAAQTKEPVRQGLVSMTGTFIDTIVICTLTGLSIVLTGAWQVDGLEGVQVTTYAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCCCCEEEHHHH
QNGLPLPKELSAFVLMLCLVFFAFTTILGWDYYSERCLEYLSGGRMKYVKVYRWIYILAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FIGPYMTVSAVWTIADIFNGLMALPNMIALFALSGVVVKETRHFFERHRNGEIED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MWNTINNFLSAVDNFVWGVPLMVLIMAGGIMLTVRLGLLQMRKLPLALKWMVKNEEGGDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCC
EITSFGALCTALSATIGTGNIVGVATAVGAGGPGALFWMVLAAFFGMATKYSEGLLAVKY
CHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
RVIGKDGHSLGGPFYYIEQGMGAKWKWLAKIFAFFGVCVGLFGIGTFSQVNGISSAVNNF
EEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
FDPKNQHTVKVLPFLGEYSWSVVIASLVLAFCVAAVLIGGVKRIASVSQIIVPFMAVIYI
CCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVLVLVVCNITAVPAAFATIVKAAFSPKAITGGAVGSFLVAMQKGVARGIFSNEAGLGS
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
APIAAAAAQTKEPVRQGLVSMTGTFIDTIVICTLTGLSIVLTGAWQVDGLEGVQVTTYAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEECCCCCCEEEHHHH
QNGLPLPKELSAFVLMLCLVFFAFTTILGWDYYSERCLEYLSGGRMKYVKVYRWIYILAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FIGPYMTVSAVWTIADIFNGLMALPNMIALFALSGVVVKETRHFFERHRNGEIED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]