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The map label for this gene is ribE [H]

Identifier: 238925476

GI number: 238925476

Start: 2984827

End: 2985546

Strand: Reverse

Name: ribE [H]

Synonym: EUBREC_3131

Alternate gene names: 238925476

Gene position: 2985546-2984827 (Counterclockwise)

Preceding gene: 238925477

Following gene: 238925475

Centisome position: 86.55

GC content: 45.42

Gene sequence:

>720_bases
ATGTTCACAGGAATAGTTGAAGAAGTTGGAACAATAGATACAATTTCACGTGGAGCAAACTCAGCAGTACTTACAATCAG
AGCTGAAAAAGTGCTAGCTGGAACAAAGGTCGGAGACAGCATAGCGGTAAACGGTATCTGCCTCACGGTCACAAGGCTCA
TGCCACATGCTTTTACAGCGGATGTCATGCACGAGACGCTAAACCGTTCATCGCTTGCAAATGCGATGAGAGGAGCACAT
GTCAATTTGGAGCGTGCGATGGCAGCAGATGGCAGATTCGGCGGGCACATAGTGTCGGGACATGTTGACGGAACAGGCAG
GATTGTGGAGGTCAAAAAGGATGACAATGCCATCTGGTATACGATACATGCCGCACCACAGCTCATTCGCTATATAGTCG
AGAAGGGCTCCGTCACAATCGATGGAATAAGCCTCACAGTCGCAAAGGTTGCAGAGGATAATTTCTCGATATCAGCGATA
CCGCACACGGTAAGCCAGACTGTGCTAAAGGACAGAAAAGTGGGAGCTGTCGTAAATCTGGAAACAGATATCATAGGAAA
ATATGTTGAGAAGCTGTTGTTTAAAGGGCAGTCGGAAGGAATAACAGAAGAAATGACAGGAGCACAGACAATTAAGGAAA
ATGTACCACATAAATCCGGCGAAAATAAAAAACAACCGGATGTAATAACAAGAGATTTCCTGGCAAAATACGGCTTTTAA

Upstream 100 bases:

>100_bases
ACATGCCGTGATGCTTGGAAACAGTAAGGTAACAAAGCTTGGTGATGATTTCCTCATCGAGAGTGATGTTGTGAATATTT
AAAAAGAAAGGCGATACACA

Downstream 100 bases:

>100_bases
CATAAAAGCACGGGTATTGTGCGGGTTTGCAAATGTGCGTGGGCTGAATGGTTAAAAAATTATGTAATTGAACAGCTTCA
AAATCATAAGAGAAAGGATA

Product: riboflavin synthase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MFTGIVEEVGTIDTISRGANSAVLTIRAEKVLAGTKVGDSIAVNGICLTVTRLMPHAFTADVMHETLNRSSLANAMRGAH
VNLERAMAADGRFGGHIVSGHVDGTGRIVEVKKDDNAIWYTIHAAPQLIRYIVEKGSVTIDGISLTVAKVAEDNFSISAI
PHTVSQTVLKDRKVGAVVNLETDIIGKYVEKLLFKGQSEGITEEMTGAQTIKENVPHKSGENKKQPDVITRDFLAKYGF

Sequences:

>Translated_239_residues
MFTGIVEEVGTIDTISRGANSAVLTIRAEKVLAGTKVGDSIAVNGICLTVTRLMPHAFTADVMHETLNRSSLANAMRGAH
VNLERAMAADGRFGGHIVSGHVDGTGRIVEVKKDDNAIWYTIHAAPQLIRYIVEKGSVTIDGISLTVAKVAEDNFSISAI
PHTVSQTVLKDRKVGAVVNLETDIIGKYVEKLLFKGQSEGITEEMTGAQTIKENVPHKSGENKKQPDVITRDFLAKYGF
>Mature_239_residues
MFTGIVEEVGTIDTISRGANSAVLTIRAEKVLAGTKVGDSIAVNGICLTVTRLMPHAFTADVMHETLNRSSLANAMRGAH
VNLERAMAADGRFGGHIVSGHVDGTGRIVEVKKDDNAIWYTIHAAPQLIRYIVEKGSVTIDGISLTVAKVAEDNFSISAI
PHTVSQTVLKDRKVGAVVNLETDIIGKYVEKLLFKGQSEGITEEMTGAQTIKENVPHKSGENKKQPDVITRDFLAKYGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=37.6344086021505, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319733, Length=227, Percent_Identity=39.647577092511, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 25756; Mature: 25756

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEEVGTIDTISRGANSAVLTIRAEKVLAGTKVGDSIAVNGICLTVTRLMPHAFTA
CCCCHHHHHCCHHHHHCCCCCEEEEEEECHHEECCCCCCCEEECHHHHHHHHHHCHHHHH
DVMHETLNRSSLANAMRGAHVNLERAMAADGRFGGHIVSGHVDGTGRIVEVKKDDNAIWY
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECCCEEEE
TIHAAPQLIRYIVEKGSVTIDGISLTVAKVAEDNFSISAIPHTVSQTVLKDRKVGAVVNL
EEEHHHHHHHHHHHCCCEEEECCEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCEEEEC
ETDIIGKYVEKLLFKGQSEGITEEMTGAQTIKENVPHKSGENKKQPDVITRDFLAKYGF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MFTGIVEEVGTIDTISRGANSAVLTIRAEKVLAGTKVGDSIAVNGICLTVTRLMPHAFTA
CCCCHHHHHCCHHHHHCCCCCEEEEEEECHHEECCCCCCCEEECHHHHHHHHHHCHHHHH
DVMHETLNRSSLANAMRGAHVNLERAMAADGRFGGHIVSGHVDGTGRIVEVKKDDNAIWY
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECCCEEEE
TIHAAPQLIRYIVEKGSVTIDGISLTVAKVAEDNFSISAIPHTVSQTVLKDRKVGAVVNL
EEEHHHHHHHHHHHCCCEEEECCEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCEEEEC
ETDIIGKYVEKLLFKGQSEGITEEMTGAQTIKENVPHKSGENKKQPDVITRDFLAKYGF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]