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The map label for this gene is ribBA [H]

Identifier: 238925475

GI number: 238925475

Start: 2983524

End: 2984720

Strand: Reverse

Name: ribBA [H]

Synonym: EUBREC_3130

Alternate gene names: 238925475

Gene position: 2984720-2983524 (Counterclockwise)

Preceding gene: 238925476

Following gene: 238925474

Centisome position: 86.52

GC content: 48.45

Gene sequence:

>1197_bases
ATGAAATTCAACACAGTAGAAGAAGCACTTGATGAGCTTCGTGCAGGAAAAATAATACTTGTCACAGATGATGAGGACAG
AGAGAATGAGGGCGATTTCATCTGTGCGGCAGAATTTGCCACAACAGAAAACATCAACTTTATGGCGATGCACGGCAAGG
GTCTGATATGCATGCCGATGTCGCAGGAATATGTAAAGAAGCTTCGTTTTCCGCAGATGGTGCAGGAAAATACAGACAAT
CATGAGACAGCATTTACAGTCTCAATAGACCATGTCTCAACAACCACAGGTATTTCCGCAGCAGAGCGCTCAATCACAGC
AATGAAATGTGTCTCTGATGACGCAAAGCCTGAGGATTTCAGACGTCCGGGCCACATGTTTCCGCTTCTTGCAAAGAAAA
ACGGCGTACTGGAGAGAAACGGACACACAGAGGCAACAGTCGATCTGTGCAGGCTTGCAGGACTAAAGGAATGTGGACTG
TGCTGTGAGATAATGCGTGAGGACGGTACCATGATGCGCACAACAGAGCTCATGGAGCTTGCAAAAAAATGGGACATCAA
GTTCATCACAATAGCGGCACTTCAGGAATACAGAAAGATACATGAAAAGCTCGTTGACCGTGTCACAGAGGTAAAGCTGC
CAACCAAATACGGCGAATTCAAGGCATATGGCTTCGTCAACAGATTAAACGGAGAGCATCATGTGGCACTTGTAAAGGGC
GAAATCGCAGATGGAAGAGATGTGCTCTGCCGAGTACACTCTGAGTGTCTCACAGGAGATGTATTCGGCTCACAGCGCTG
TGACTGTGGCCAGCAGCTTGAGGCGGCCATGAGACAGATAGACGAGGAGGGCAGAGGAATACTGCTCTACCTGCGTCAGG
AGGGCAGAGGCATCGGCCTGATAAATAAGCTGAAGGCCTATGAGCTTCAGGAACAGGGTATGGATACACTCGAAGCAAAC
CTCGCACTCGGCTTTGCCGGAGATGAGCGCGAGTACTATATCGGAGCCCAGATATTAAGAGAGCTTGGCGTACACAGCCT
GCGCCTTCTCACAAATAACCCTGACAAGGTATACCAGCTCTCAGAATTTGGTATGGAAATCAGCGAGCGAGTGCCAATCC
AGATGAGCGCCACAGCGCATGACCTGTTTTATCTGAAAACAAAGCAGAACCGCATGGGACATATACTCTCATACTAG

Upstream 100 bases:

>100_bases
AGCACGGGTATTGTGCGGGTTTGCAAATGTGCGTGGGCTGAATGGTTAAAAAATTATGTAATTGAACAGCTTCAAAATCA
TAAGAGAAAGGATATAAAAT

Downstream 100 bases:

>100_bases
CAAAAATAAAAAAACAAAAGGAGAACAAAAATGAACGTAATCGAAGGAAAAATGACACAGGATGGAATCAAGGTAGGAAT
CGTAGTAGCAAGATTCAATG

Product: riboflavin biosynthesis; GTP-cyclohydrolase II

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKFNTVEEALDELRAGKIILVTDDEDRENEGDFICAAEFATTENINFMAMHGKGLICMPMSQEYVKKLRFPQMVQENTDN
HETAFTVSIDHVSTTTGISAAERSITAMKCVSDDAKPEDFRRPGHMFPLLAKKNGVLERNGHTEATVDLCRLAGLKECGL
CCEIMREDGTMMRTTELMELAKKWDIKFITIAALQEYRKIHEKLVDRVTEVKLPTKYGEFKAYGFVNRLNGEHHVALVKG
EIADGRDVLCRVHSECLTGDVFGSQRCDCGQQLEAAMRQIDEEGRGILLYLRQEGRGIGLINKLKAYELQEQGMDTLEAN
LALGFAGDEREYYIGAQILRELGVHSLRLLTNNPDKVYQLSEFGMEISERVPIQMSATAHDLFYLKTKQNRMGHILSY

Sequences:

>Translated_398_residues
MKFNTVEEALDELRAGKIILVTDDEDRENEGDFICAAEFATTENINFMAMHGKGLICMPMSQEYVKKLRFPQMVQENTDN
HETAFTVSIDHVSTTTGISAAERSITAMKCVSDDAKPEDFRRPGHMFPLLAKKNGVLERNGHTEATVDLCRLAGLKECGL
CCEIMREDGTMMRTTELMELAKKWDIKFITIAALQEYRKIHEKLVDRVTEVKLPTKYGEFKAYGFVNRLNGEHHVALVKG
EIADGRDVLCRVHSECLTGDVFGSQRCDCGQQLEAAMRQIDEEGRGILLYLRQEGRGIGLINKLKAYELQEQGMDTLEAN
LALGFAGDEREYYIGAQILRELGVHSLRLLTNNPDKVYQLSEFGMEISERVPIQMSATAHDLFYLKTKQNRMGHILSY
>Mature_398_residues
MKFNTVEEALDELRAGKIILVTDDEDRENEGDFICAAEFATTENINFMAMHGKGLICMPMSQEYVKKLRFPQMVQENTDN
HETAFTVSIDHVSTTTGISAAERSITAMKCVSDDAKPEDFRRPGHMFPLLAKKNGVLERNGHTEATVDLCRLAGLKECGL
CCEIMREDGTMMRTTELMELAKKWDIKFITIAALQEYRKIHEKLVDRVTEVKLPTKYGEFKAYGFVNRLNGEHHVALVKG
EIADGRDVLCRVHSECLTGDVFGSQRCDCGQQLEAAMRQIDEEGRGILLYLRQEGRGIGLINKLKAYELQEQGMDTLEAN
LALGFAGDEREYYIGAQILRELGVHSLRLLTNNPDKVYQLSEFGMEISERVPIQMSATAHDLFYLKTKQNRMGHILSY

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=56.9948186528497, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789420, Length=203, Percent_Identity=47.2906403940887, Blast_Score=196, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=40.2912621359223, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319438, Length=172, Percent_Identity=45.3488372093023, Blast_Score=135, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 45020; Mature: 45020

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNTVEEALDELRAGKIILVTDDEDRENEGDFICAAEFATTENINFMAMHGKGLICMPM
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECC
SQEYVKKLRFPQMVQENTDNHETAFTVSIDHVSTTTGISAAERSITAMKCVSDDAKPEDF
CHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
RRPGHMFPLLAKKNGVLERNGHTEATVDLCRLAGLKECGLCCEIMREDGTMMRTTELMEL
CCCCCCEEEEECCCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHH
AKKWDIKFITIAALQEYRKIHEKLVDRVTEVKLPTKYGEFKAYGFVNRLNGEHHVALVKG
HHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCEEEEEEEC
EIADGRDVLCRVHSECLTGDVFGSQRCDCGQQLEAAMRQIDEEGRGILLYLRQEGRGIGL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
INKLKAYELQEQGMDTLEANLALGFAGDEREYYIGAQILRELGVHSLRLLTNNPDKVYQL
HHHHHHHHHHHCCCCHHCCCEEEEECCCCCCEEHHHHHHHHCCCCEEEEEECCCHHHEEH
SEFGMEISERVPIQMSATAHDLFYLKTKQNRMGHILSY
HHHCCCHHHCCCEEEECCEEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKFNTVEEALDELRAGKIILVTDDEDRENEGDFICAAEFATTENINFMAMHGKGLICMPM
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECC
SQEYVKKLRFPQMVQENTDNHETAFTVSIDHVSTTTGISAAERSITAMKCVSDDAKPEDF
CHHHHHHCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
RRPGHMFPLLAKKNGVLERNGHTEATVDLCRLAGLKECGLCCEIMREDGTMMRTTELMEL
CCCCCCEEEEECCCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHH
AKKWDIKFITIAALQEYRKIHEKLVDRVTEVKLPTKYGEFKAYGFVNRLNGEHHVALVKG
HHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCEEEEEEEC
EIADGRDVLCRVHSECLTGDVFGSQRCDCGQQLEAAMRQIDEEGRGILLYLRQEGRGIGL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCH
INKLKAYELQEQGMDTLEANLALGFAGDEREYYIGAQILRELGVHSLRLLTNNPDKVYQL
HHHHHHHHHHHCCCCHHCCCEEEEECCCCCCEEHHHHHHHHCCCCEEEEEECCCHHHEEH
SEFGMEISERVPIQMSATAHDLFYLKTKQNRMGHILSY
HHHCCCHHHCCCEEEECCEEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA