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The map label for this gene is yibK [C]

Identifier: 238925347

GI number: 238925347

Start: 2880447

End: 2880950

Strand: Reverse

Name: yibK [C]

Synonym: EUBREC_3002

Alternate gene names: 238925347

Gene position: 2880950-2880447 (Counterclockwise)

Preceding gene: 238925348

Following gene: 238925346

Centisome position: 83.51

GC content: 46.63

Gene sequence:

>504_bases
ATGGCAAAGCTGAACATAGTGTTACATGAGCCGGAAATTCCGGCAAATACGGGAAATATCGGAAGAACCTGCGTCGCGAC
AGGCACGAGGCTTCATCTTATAGAGCCGCTTGGATTCTCACTTTCAGAGAAGGCACTAAAGAGAGCCGGAATGGATTACT
GGAAGGACCTCGACGTCACCACATATGTGGACTGGGAGGATTTCCTTGAGAGAAATCCGGGCGCAAAGATATATTATGCG
ACAACAAAGGGCAGACATGTGTACTCTGATGCTCATTTTGAGCCAGACTGCTATATTATGTTTGGCAAGGAAAGCGCAGG
CATTCCGGAGGAGATACTAAAGGAGCATCCGGATGAGTGCATCAGGATACCGATGATAGGTGAGACAAGGTCACTTAATT
TGTCAAATTCAGTAGCTATCGTGCTCTACGAGGCATTGAGACAGAATAATTTTGATCATATGAGGCTTGAGGGAGAGCTT
CATCACCTGAGCTGGGATGACTAA

Upstream 100 bases:

>100_bases
GCTTGATGATTTAAAGGAGGCGCTCGGCCGCATTGAGGAGTTTGTCACAGAGCTTCGCGCCGGGATGGATAATAAATAGT
TTAAATTGACAGGAGACAAA

Downstream 100 bases:

>100_bases
ATCAAGCGGCAAAGTGCATATTTTTGGCAGCTATTTTGGCTTTAACAGCTATGATAATTGTAAATACGGTAGATTTTTAG
GAAAAAGAAATGGGCATAAT

Product: RNA methyltransferase, TrmH family, group 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MAKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEKALKRAGMDYWKDLDVTTYVDWEDFLERNPGAKIYYA
TTKGRHVYSDAHFEPDCYIMFGKESAGIPEEILKEHPDECIRIPMIGETRSLNLSNSVAIVLYEALRQNNFDHMRLEGEL
HHLSWDD

Sequences:

>Translated_167_residues
MAKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEKALKRAGMDYWKDLDVTTYVDWEDFLERNPGAKIYYA
TTKGRHVYSDAHFEPDCYIMFGKESAGIPEEILKEHPDECIRIPMIGETRSLNLSNSVAIVLYEALRQNNFDHMRLEGEL
HHLSWDD
>Mature_166_residues
AKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEKALKRAGMDYWKDLDVTTYVDWEDFLERNPGAKIYYAT
TKGRHVYSDAHFEPDCYIMFGKESAGIPEEILKEHPDECIRIPMIGETRSLNLSNSVAIVLYEALRQNNFDHMRLEGELH
HLSWDD

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=146, Percent_Identity=50.6849315068493, Blast_Score=147, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19090; Mature: 18959

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEKALKRAGMDYWKDLDVT
CCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHCCCCHHHCCCEE
TYVDWEDFLERNPGAKIYYATTKGRHVYSDAHFEPDCYIMFGKESAGIPEEILKEHPDEC
EEECHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHCCCCE
IRIPMIGETRSLNLSNSVAIVLYEALRQNNFDHMRLEGELHHLSWDD
EEEEECCCCCCCCCCCCCHHEEEHHHHHCCCCEEEEECEEEECCCCC
>Mature Secondary Structure 
AKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEKALKRAGMDYWKDLDVT
CEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHCCCCHHHCCCEE
TYVDWEDFLERNPGAKIYYATTKGRHVYSDAHFEPDCYIMFGKESAGIPEEILKEHPDEC
EEECHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHCCCCE
IRIPMIGETRSLNLSNSVAIVLYEALRQNNFDHMRLEGELHHLSWDD
EEEEECCCCCCCCCCCCCHHEEEHHHHHCCCCEEEEECEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]