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The map label for this gene is trpE [H]

Identifier: 238925325

GI number: 238925325

Start: 2862647

End: 2864182

Strand: Reverse

Name: trpE [H]

Synonym: EUBREC_2980

Alternate gene names: 238925325

Gene position: 2864182-2862647 (Counterclockwise)

Preceding gene: 238925326

Following gene: 238925324

Centisome position: 83.03

GC content: 44.47

Gene sequence:

>1536_bases
TTGAAAAGGCGAATTCGAGCTGTATGTTGGGCATTTGTTCACACAAACACAAAATTTGGAGGTAAGCATATGAAAATTTC
ACCTACACTGACCGAAGTTAAAGAAATAGCAAAAACCGGGAAGTATGACATTCTCCCTGTAAGCTGTGAGATATTATCAG
ATTTTATCACACCTATAGAGGCTATGAGGATTCTGCAGAATGTTTCAACACATTGTTATCTGCTTGAATCAGCACAAGCC
AATGAAGCCTGGGGAAGATATACATTTTTGGGATATGATCCAAAGCTTGAGATTAACTGTATAGATGGTCACATGAAGCT
TGGAAAGCTTTCGGTGGAGACGGAAAATCCTTCAGAGTACATCAGACAGATTCTTTCAGAGTACAAGAGTCCGAAATTTG
ATTATCTGCCTTCTTTTACAGGTGGCCTTGTGGGTTATTTTTCGTATGATTATCTGGGCTACAAGGAGAAAAGCATCAGA
GGAAAAGCAAGGGACACGGAGGATTTCAAGGATGTTGATCTGATGCTTTTTGATAAGGTAATAGCATTTGACAATCTGTG
CCAAAAGATAATACTAATCGCAAATATGTCACTTGATGCTCCGGAAACAGGCTACAACAAAGCGATTGTTGAGCTAAAGC
AGCTTAGGGAGCTTCTGATGCACGGAGAGAAAAAACAGAATATGGGTGGCAAGCTGCTGGGTGAGGTCACACCGCTTTTT
GATAAGGAGCAGTATTGTGAGATGGTCGAGAAGGCAAAGCACCATATCAAAGAGGGGGATATTTTCCAGATAGTTTTATC
CAACAGATTGAGCGCACCGTTTGAGGGAAGCCTTTTTGATACCTACCGCGTGCTTCGAACGATAAATCCTTCGCCGTACA
TGTTTTATTTTTCGGGAACCGATGTGGAGGTTGCGGGAGCATCTCCGGAGACACTTGTGAAGCTTGAAAATGGAGTGCTT
CACACATTCCCGCTGGCAGGCACGAGGCCGAGAGGTGCCACAGCAGAGGAGGACAAAAAGCTTGAGGAAGGGCTGCTTAA
GGACGAAAAAGAGCTCGCAGAGCACAATATGCTCGTGGACCTAGGAAGAAATGACCTCGGAAAGGTAAGTAAATTCGGAA
CTGTAAAAGTAGAAAAGCTTCATTCCATAGAGAGATATTCACATGTGATGCATATAGGCTCGACAGTGCGTGGAGAGATA
AGAGAAGAGTACGACGCACTCGATGCAATAGAGGCAGTGCTCCCGGCAGGAACACTTTCAGGAGCACCAAAGATCAGAGC
GTGCCAGCTTATCGGAGAGCTTGAGGACAACAAGCGAGGCATTTACGGAGGGGCGATCGGATATATAGATTTTACAGGAA
ACATGGACACATGTATTGCAATCCGCATTGCCTACAAGAAAAACGGCAAGGTTTTCGTGAGAAGCGGAGCGGGAATAGTT
GCGGATTCAGATCCTGAGAAGGAATTTCAGGAGTGCATAAACAAGGCAAAGGCTTCTCTTAAGGCACTGGAAGTCTCACA
GGAGGTGGAAGAATGA

Upstream 100 bases:

>100_bases
GGAGTTTGCAAGTCTGCATGCTCGCATGCAAGCTTGCGAACGAACATTGGACTGCACAACATAAAGCGAGAATTGTAGCA
GCGCAAGCTGCGGAGAAGCC

Downstream 100 bases:

>100_bases
TTTTACTGATTGATAACTACGACAGCTTTTCCTACAATCTGTATCAGCTAATCGGCGAGATAGAGCCTGATATCAGAGTA
ATCAGAAATGATGAGATGAC

Product: anthranilate/para-aminobenzoate synthase component I, TrpE

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKRRIRAVCWAFVHTNTKFGGKHMKISPTLTEVKEIAKTGKYDILPVSCEILSDFITPIEAMRILQNVSTHCYLLESAQA
NEAWGRYTFLGYDPKLEINCIDGHMKLGKLSVETENPSEYIRQILSEYKSPKFDYLPSFTGGLVGYFSYDYLGYKEKSIR
GKARDTEDFKDVDLMLFDKVIAFDNLCQKIILIANMSLDAPETGYNKAIVELKQLRELLMHGEKKQNMGGKLLGEVTPLF
DKEQYCEMVEKAKHHIKEGDIFQIVLSNRLSAPFEGSLFDTYRVLRTINPSPYMFYFSGTDVEVAGASPETLVKLENGVL
HTFPLAGTRPRGATAEEDKKLEEGLLKDEKELAEHNMLVDLGRNDLGKVSKFGTVKVEKLHSIERYSHVMHIGSTVRGEI
REEYDALDAIEAVLPAGTLSGAPKIRACQLIGELEDNKRGIYGGAIGYIDFTGNMDTCIAIRIAYKKNGKVFVRSGAGIV
ADSDPEKEFQECINKAKASLKALEVSQEVEE

Sequences:

>Translated_511_residues
MKRRIRAVCWAFVHTNTKFGGKHMKISPTLTEVKEIAKTGKYDILPVSCEILSDFITPIEAMRILQNVSTHCYLLESAQA
NEAWGRYTFLGYDPKLEINCIDGHMKLGKLSVETENPSEYIRQILSEYKSPKFDYLPSFTGGLVGYFSYDYLGYKEKSIR
GKARDTEDFKDVDLMLFDKVIAFDNLCQKIILIANMSLDAPETGYNKAIVELKQLRELLMHGEKKQNMGGKLLGEVTPLF
DKEQYCEMVEKAKHHIKEGDIFQIVLSNRLSAPFEGSLFDTYRVLRTINPSPYMFYFSGTDVEVAGASPETLVKLENGVL
HTFPLAGTRPRGATAEEDKKLEEGLLKDEKELAEHNMLVDLGRNDLGKVSKFGTVKVEKLHSIERYSHVMHIGSTVRGEI
REEYDALDAIEAVLPAGTLSGAPKIRACQLIGELEDNKRGIYGGAIGYIDFTGNMDTCIAIRIAYKKNGKVFVRSGAGIV
ADSDPEKEFQECINKAKASLKALEVSQEVEE
>Mature_511_residues
MKRRIRAVCWAFVHTNTKFGGKHMKISPTLTEVKEIAKTGKYDILPVSCEILSDFITPIEAMRILQNVSTHCYLLESAQA
NEAWGRYTFLGYDPKLEINCIDGHMKLGKLSVETENPSEYIRQILSEYKSPKFDYLPSFTGGLVGYFSYDYLGYKEKSIR
GKARDTEDFKDVDLMLFDKVIAFDNLCQKIILIANMSLDAPETGYNKAIVELKQLRELLMHGEKKQNMGGKLLGEVTPLF
DKEQYCEMVEKAKHHIKEGDIFQIVLSNRLSAPFEGSLFDTYRVLRTINPSPYMFYFSGTDVEVAGASPETLVKLENGVL
HTFPLAGTRPRGATAEEDKKLEEGLLKDEKELAEHNMLVDLGRNDLGKVSKFGTVKVEKLHSIERYSHVMHIGSTVRGEI
REEYDALDAIEAVLPAGTLSGAPKIRACQLIGELEDNKRGIYGGAIGYIDFTGNMDTCIAIRIAYKKNGKVFVRSGAGIV
ADSDPEKEFQECINKAKASLKALEVSQEVEE

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=421, Percent_Identity=37.29216152019, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1788114, Length=439, Percent_Identity=30.2961275626424, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87082077, Length=268, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786809, Length=254, Percent_Identity=27.5590551181102, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320935, Length=503, Percent_Identity=36.779324055666, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324361, Length=403, Percent_Identity=26.7990074441687, Blast_Score=113, Evalue=5e-26,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57338; Mature: 57338

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRIRAVCWAFVHTNTKFGGKHMKISPTLTEVKEIAKTGKYDILPVSCEILSDFITPIE
CCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
AMRILQNVSTHCYLLESAQANEAWGRYTFLGYDPKLEINCIDGHMKLGKLSVETENPSEY
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCEEECEEEEECCCHHHH
IRQILSEYKSPKFDYLPSFTGGLVGYFSYDYLGYKEKSIRGKARDTEDFKDVDLMLFDKV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
IAFDNLCQKIILIANMSLDAPETGYNKAIVELKQLRELLMHGEKKQNMGGKLLGEVTPLF
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHCCCC
DKEQYCEMVEKAKHHIKEGDIFQIVLSNRLSAPFEGSLFDTYRVLRTINPSPYMFYFSGT
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECC
DVEVAGASPETLVKLENGVLHTFPLAGTRPRGATAEEDKKLEEGLLKDEKELAEHNMLVD
CEEEECCCCHHEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCEEEE
LGRNDLGKVSKFGTVKVEKLHSIERYSHVMHIGSTVRGEIREEYDALDAIEAVLPAGTLS
CCCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GAPKIRACQLIGELEDNKRGIYGGAIGYIDFTGNMDTCIAIRIAYKKNGKVFVRSGAGIV
CCCCHHHHHHHHHHCCCCCCEECCEEEEEEECCCCCEEEEEEEEEECCCEEEEEECCCEE
ADSDPEKEFQECINKAKASLKALEVSQEVEE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRRIRAVCWAFVHTNTKFGGKHMKISPTLTEVKEIAKTGKYDILPVSCEILSDFITPIE
CCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
AMRILQNVSTHCYLLESAQANEAWGRYTFLGYDPKLEINCIDGHMKLGKLSVETENPSEY
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCEEECEEEEECCCHHHH
IRQILSEYKSPKFDYLPSFTGGLVGYFSYDYLGYKEKSIRGKARDTEDFKDVDLMLFDKV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHH
IAFDNLCQKIILIANMSLDAPETGYNKAIVELKQLRELLMHGEKKQNMGGKLLGEVTPLF
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHCCCC
DKEQYCEMVEKAKHHIKEGDIFQIVLSNRLSAPFEGSLFDTYRVLRTINPSPYMFYFSGT
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECC
DVEVAGASPETLVKLENGVLHTFPLAGTRPRGATAEEDKKLEEGLLKDEKELAEHNMLVD
CEEEECCCCHHEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCEEEE
LGRNDLGKVSKFGTVKVEKLHSIERYSHVMHIGSTVRGEIREEYDALDAIEAVLPAGTLS
CCCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GAPKIRACQLIGELEDNKRGIYGGAIGYIDFTGNMDTCIAIRIAYKKNGKVFVRSGAGIV
CCCCHHHHHHHHHHCCCCCCEECCEEEEEEECCCCCEEEEEEEEEECCCEEEEEECCCEE
ADSDPEKEFQECINKAKASLKALEVSQEVEE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]