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The map label for this gene is potB [H]

Identifier: 238924523

GI number: 238924523

Start: 2013339

End: 2014163

Strand: Direct

Name: potB [H]

Synonym: EUBREC_2165

Alternate gene names: 238924523

Gene position: 2013339-2014163 (Clockwise)

Preceding gene: 238924522

Following gene: 238924524

Centisome position: 58.36

GC content: 45.21

Gene sequence:

>825_bases
ATGTCTAAAATAAAGAAACAGCTGCTCGCAGGGCCGTACCTCATATGGATTATCGGATTTATCATACTTCCGCTTTTCAT
GATTGTGTACTATGCATTTAAAGGCTCTGACGGAGGCTTCACATTTGAATATGTGGCTGCAATAGCACAGCACACCAATA
TCAAGGCACTGCTTTTATCCCTACGGCTTGGAATCATATGCACCATAATATGCCTGGTGCTGTCCTATCCGCTTGCGATG
ATTTTAAACGGCTTAAAGATTAAAAACCAAAGCTTTGTGGTATTTGTTTTCATGCTTCCTATGTGGATGAACTTTATGCT
TCGAATACTCGCATGGAAGCAGCTTTTGTCCAAAAACGGTGTCATCAACTCGATACTCACCACTCTCGGACTGCCGGGCT
TCAACATCATGAATACCTCCGGAGCAGTCGTGCTTGGCATGGTCTATGACTTTCTGCCATTCATGCTGCTGCCTATATAC
AACTCCATGACCAGAATCAAAAACGACTGGATTGAGGCGGCTCTTGACCTCGGTGCAAACAAAGTCACAATACTTTTCAA
GATTATACTGCCACTTACAGTTTCCGGTGTCATAAGCGGAATCGTAATGGTTTTCGTGCCATCACTCACATCCTTTGCCA
TCTCACAGATACTCGGTGGCGGCAAGGTCCTGCTCATCGGAAATATCATAGAACAGGATTTCGTCCATGGCTCGCAGTGG
GGCGCAGGAAGCGGTCTCTCACTCGTGCTCATGGTATTCGTATTAATCAGCATGGCACTCGTCAATCTGTTTGATAAGGA
AGGAGACCAGAGCGCATTATGGTAA

Upstream 100 bases:

>100_bases
TATGTAGATGTCGGCAGTCATGTGGGTATCAAGGTGATTCCATTTAATATCCAGGTAATGCACAAGCCTGAGTCCTCAGA
TGAAAAGGCGGTGGAGATAG

Downstream 100 bases:

>100_bases
AAAAAATAGCATCAAGGGTATATATGGGACTGATTTTTTTGTTTCTGTATCTGCCTATCGTTGTATTAATAGTACTCTCC
TTCAACAACTCCAAATCCAA

Product: ABC-type spermidine/putrescine transport system, permease protein 1

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSKIKKQLLAGPYLIWIIGFIILPLFMIVYYAFKGSDGGFTFEYVAAIAQHTNIKALLLSLRLGIICTIICLVLSYPLAM
ILNGLKIKNQSFVVFVFMLPMWMNFMLRILAWKQLLSKNGVINSILTTLGLPGFNIMNTSGAVVLGMVYDFLPFMLLPIY
NSMTRIKNDWIEAALDLGANKVTILFKIILPLTVSGVISGIVMVFVPSLTSFAISQILGGGKVLLIGNIIEQDFVHGSQW
GAGSGLSLVLMVFVLISMALVNLFDKEGDQSALW

Sequences:

>Translated_274_residues
MSKIKKQLLAGPYLIWIIGFIILPLFMIVYYAFKGSDGGFTFEYVAAIAQHTNIKALLLSLRLGIICTIICLVLSYPLAM
ILNGLKIKNQSFVVFVFMLPMWMNFMLRILAWKQLLSKNGVINSILTTLGLPGFNIMNTSGAVVLGMVYDFLPFMLLPIY
NSMTRIKNDWIEAALDLGANKVTILFKIILPLTVSGVISGIVMVFVPSLTSFAISQILGGGKVLLIGNIIEQDFVHGSQW
GAGSGLSLVLMVFVLISMALVNLFDKEGDQSALW
>Mature_273_residues
SKIKKQLLAGPYLIWIIGFIILPLFMIVYYAFKGSDGGFTFEYVAAIAQHTNIKALLLSLRLGIICTIICLVLSYPLAMI
LNGLKIKNQSFVVFVFMLPMWMNFMLRILAWKQLLSKNGVINSILTTLGLPGFNIMNTSGAVVLGMVYDFLPFMLLPIYN
SMTRIKNDWIEAALDLGANKVTILFKIILPLTVSGVISGIVMVFVPSLTSFAISQILGGGKVLLIGNIIEQDFVHGSQWG
AGSGLSLVLMVFVLISMALVNLFDKEGDQSALW

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=245, Percent_Identity=36.3265306122449, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787080, Length=219, Percent_Identity=35.1598173515982, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787713, Length=238, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30165; Mature: 30034

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKKQLLAGPYLIWIIGFIILPLFMIVYYAFKGSDGGFTFEYVAAIAQHTNIKALLLS
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHH
LRLGIICTIICLVLSYPLAMILNGLKIKNQSFVVFVFMLPMWMNFMLRILAWKQLLSKNG
HHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VINSILTTLGLPGFNIMNTSGAVVLGMVYDFLPFMLLPIYNSMTRIKNDWIEAALDLGAN
HHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KVTILFKIILPLTVSGVISGIVMVFVPSLTSFAISQILGGGKVLLIGNIIEQDFVHGSQW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCC
GAGSGLSLVLMVFVLISMALVNLFDKEGDQSALW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SKIKKQLLAGPYLIWIIGFIILPLFMIVYYAFKGSDGGFTFEYVAAIAQHTNIKALLLS
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHH
LRLGIICTIICLVLSYPLAMILNGLKIKNQSFVVFVFMLPMWMNFMLRILAWKQLLSKNG
HHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VINSILTTLGLPGFNIMNTSGAVVLGMVYDFLPFMLLPIYNSMTRIKNDWIEAALDLGAN
HHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KVTILFKIILPLTVSGVISGIVMVFVPSLTSFAISQILGGGKVLLIGNIIEQDFVHGSQW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCC
GAGSGLSLVLMVFVLISMALVNLFDKEGDQSALW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]